Chitosan tamarind-based nanoparticles as a promising approach for topical application of curcumin intended for burn healing: in vitro and in vivo study

References

• Nacer Khodja A, Mahlous M, Tahtat D, et al. Evaluation of healing activity of PVA/chitosan hydrogels on deep second degree burn: pharmacological and toxicological tests. Burns. 2013;39(1):98–104. doi: 10.1016/j.burns.2012.05.021.
   PubMed Web of Science ®Google Scholar
• Jaiprakash B, Chandramohan, Reddy DN. Burn wound healing activity of Euphorbia hirta. Ancient Sci Life. 2006;25(3–4):16–18.
   PubMedGoogle Scholar
• Shanmugasundaram N, Uma TS, Ramyaa Lakshmi TS, et al. Efficiency of controlled topical delivery of silver sulfadiazine in infected burn wounds. J Biomed Mater Res A. 2009;89(2):472–482. doi: 10.1002/jbm.a.31997.
   PubMed Web of Science ®Google Scholar
• Panahi Y, Fazlolahzadeh O, Atkin SL, et al. Evidence of curcumin and curcumin analogue effects in skin diseases: a narrative review. J Cell Physiol. 2019;234(2):1165–1178. doi: 10.1002/jcp.27096.
   PubMed Web of Science ®Google Scholar
• Mehrabani D, Farjam M, Geramizadeh B, et al. The healing effect of curcumin on burn wounds in rat. World J Plast Surg. 2015;4(1):29–35.
   PubMedGoogle Scholar
• Nawaz A, Safdar M, Arshad MS, et al. Formulation development and in vitro permeability of curcumin films using different penetration enhancers. Drug Deliv Lett. 2018;8(1):78–84. doi: 10.2174/2210303107666171121161647.
   Google Scholar
• Alven S, Nqoro X, Aderibigbe BA. Polymer-based materials loaded with curcumin for wound healing applications. Polymers. 2020;12(10):2286. doi: 10.3390/polym12102286.
   PubMed Web of Science ®Google Scholar
• Zheng D, Huang C, Huang H, et al. Antibacterial mechanism of curcumin: a review. Chem Biodivers. 2020;17(8):e2000171.
   PubMed Web of Science ®Google Scholar
• Jana S, Saha A, Nayak AK, et al. Aceclofenac-loaded chitosan-tamarind seed polysaccharide interpenetrating polymeric network microparticles. Colloids Surf B Biointerfaces. 2013;105:303–309. doi: 10.1016/j.colsurfb.2013.01.013.
   PubMed Web of Science ®Google Scholar
• Dutta PK, Dutta J, Tripathi VS. Chitin and chitosan: chemistry, properties and applications. J Sci Ind Res India. 2004;63:20–31.
   Web of Science ®Google Scholar
• Mehta P, Rasekh M, Patel M, et al. Applications of electrical, centrifugal, and pressurised emerging technologies for fibrous structure engineering in drug delivery, regenerative medicine and theranostics. Antioxidants. 2021;175:113823.
   Google Scholar
• Ardean C, Davidescu CM, Nemeş NS, et al. Factors influencing the antibacterial activity of chitosan and chitosan modified by functionalization. Int J Mol Sci. 2021;22(14):7449. doi: 10.3390/ijms22147449.
   PubMed Web of Science ®Google Scholar
• Alavi M, Hamblin MR, Martinez F, et al. Micro and nanoformulations of insulin: new approaches. Nano Micro Biosyst. 2022;1(1):1–7.
   Google Scholar
• El-Feky GS, Sharaf SS, El Shafei A, et al. Using chitosan nanoparticles as drug carriers for the development of a silver sulfadiazine wound dressing. Carbohydr Polym. 2017;158:11–19. doi: 10.1016/j.carbpol.2016.11.054.
   PubMed Web of Science ®Google Scholar
• Ak B, Rimi D. A new nasal drug delivery system for diazepam using natural mucoadhesive polysaccharide obtained from tamarind seeds. Saudi Pharm J. 2006;14(2):115–119.
   Google Scholar
• Malviya R, Raj S, Fuloria S, et al. Evaluation of antitumor efficacy of chitosan-tamarind gum polysaccharide polyelectrolyte complex stabilized nanoparticles of simvastatin. Int J Nanomedicine. 2021;16:2533–2553. doi: 10.2147/IJN.S300991.
   PubMed Web of Science ®Google Scholar
• Mohanty C, Sahoo SK. Curcumin and its topical formulations for wound healing applications. Drug Discov Today. 2017;22(10):1582–1592. doi: 10.1016/j.drudis.2017.07.001.
   PubMed Web of Science ®Google Scholar
• Mofazzal Jahromi MA, Sahandi Zangabad P, Moosavi Basri SM, et al. Nanomedicine and advanced technologies for burns: preventing infection and facilitating wound healing. Adv Drug Deliv Rev. 2018;123:33–64. doi: 10.1016/j.addr.2017.08.001.
   PubMed Web of Science ®Google Scholar
• Zaghloul N, El Hoffy NM, Mahmoud AA, et al. Cyclodextrin stabilized freeze-dried silica/chitosan nanoparticles for improved terconazole ocular bioavailability. Pharmaceutics. 2022;14(3):470. doi: 10.3390/pharmaceutics14030470.
   PubMed Web of Science ®Google Scholar
• Musso YS, Salgado PR, Mauri AN. Smart edible films based on gelatin and curcumin. Food Hydrocolloids. 2017;66:8–15. doi: 10.1016/j.foodhyd.2016.11.007.
   Web of Science ®Google Scholar
• Briones AV, Sato T. Encapsulation of glucose oxidase (GOD) in polyelectrolyte complexes of chitosan–carrageenan. React Funct Polym. 2010;70(1):19–27. doi: 10.1016/j.reactfunctpolym.2009.09.009.
   Web of Science ®Google Scholar
• Mende M, Schwarz S, Petzold G, et al. Destabilization of model silica dispersions by polyelectrolyte complex particles with different charge excess, hydrophobicity, and particle size. J Appl Polym Sci. 2007;103(6):3776–3784. doi: 10.1002/app.25573.
   Web of Science ®Google Scholar
• Khalid A, Ahmed N, Qindeel M, et al. Development of novel biopolymer-based nanoparticles loaded cream for potential treatment of topical fungal infections. Drug Dev Ind Pharm. 2021;47(7):1090–1099. doi: 10.1080/03639045.2021.1957914.
   PubMed Web of Science ®Google Scholar
• Abo-Elseoud WS, Hassan ML, Sabaa MW, et al. Chitosan nanoparticles/cellulose nanocrystals nanocomposites as a carrier system for the controlled release of repaglinide. Int J Biol Macromol. 2018;111:604–613. doi: 10.1016/j.ijbiomac.2018.01.044.
   PubMed Web of Science ®Google Scholar
• Khalil RM, El Arini SK, AbouSamra MM, et al. Development of lecithin/chitosan nanoparticles for promoting topical delivery of propranolol hydrochloride: design, optimization and in-vivo evaluation. J Pharm Sci. 2021;110(3):1337–1348. doi: 10.1016/j.xphs.2020.11.025.
   PubMed Web of Science ®Google Scholar
• Ammar HO, Ghorab MM, Mostafa DM, et al. Development of folic acid-loaded nanostructured lipid carriers for topical delivery: preparation, characterisation and ex vivo investigation. J Microencapsul. 2020;37(5):366–383. doi: 10.1080/02652048.2020.1761904.
   PubMed Web of Science ®Google Scholar
• Shalaby ES, Shalaby SI. Optimization of folic acid Span 60-organogel to enhance its in vitro and in vivo photoprotection: a comparative study. Ther Deliv. 2023;13(11):517–530. doi: 10.4155/tde-2022-0048.
   Web of Science ®Google Scholar
• Makoni PA, Wa Kasongo K, Walker RB. Short term stability testing of efavirenz-loaded solid lipid nanoparticle (SLN) and nanostructured lipid carrier (NLC) dispersions. Pharmaceutics. 2019;11(8):397. doi: 10.3390/pharmaceutics11080397.
   PubMed Web of Science ®Google Scholar
• Etman M, Nada H, Nada A, et al. Formulation of sustained-release ketorolac tromethamine pellets. J Pharm Technol. 2008;32:12.
   Google Scholar
• Qindeel M, Khan D, Ahmed N, et al. Surfactant-free, self-assembled nanomicelles-based transdermal hydrogel for safe and targeted delivery of methotrexate against rheumatoid arthritis. ACS Nano. 2020;14:(4):4662–4681. doi: 10.1021/acsnano.0c00364.
   PubMed Web of Science ®Google Scholar
• Gong C, Lu C, Li B, et al. Injectable dopamine-modified poly (α,β-aspartic acid) nanocomposite hydrogel as bioadhesive drug delivery system. J Biomed Mater Res A. 2017;105(4):1000–1008. doi: 10.1002/jbm.a.35931.
   PubMed Web of Science ®Google Scholar
• Monteiro CRAV, do Carmo MS, Melo BO, et al. In vitro antimicrobial activity and probiotic potential of Bifidobacterium and Lactobacillus against species of Clostridium. Nutrients. 2019;11(2):448. doi: 10.3390/nu11020448.
   PubMed Web of Science ®Google Scholar
• Brand-Williams W, Cuvelier M-E, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol. 1995;28(1):25–30. doi: 10.1016/S0023-6438(95)80008-5.
   Web of Science ®Google Scholar
• Cai EZ, Ang CH, Raju A, et al. Creation of consistent burn wounds: a rat model. Arch Plast Surg. 2014;41(4):317–324. doi: 10.5999/aps.2014.41.4.317.
   PubMedGoogle Scholar
• Asfour MH, Elmotasem H, Mostafa DM, et al. Chitosan based pickering emulsion as a promising approach for topical application of rutin in a solubilized form intended for wound healing: in vitro and in vivo study. Int J Pharm. 2017;534(1–2):325–338. doi: 10.1016/j.ijpharm.2017.10.044.
   PubMedGoogle Scholar
• Jollow D, Mitchell J, Zampaglione N, et al. Bromobenzene-induced liver necrosis. Protective role of glutathione and evidence for 3,4-bromobenzene oxide as the hepatotoxic metabolite. Pharmacology. 1974;11(3):151–169. doi: 10.1159/000136485.
   PubMed Web of Science ®Google Scholar
• Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351–358. doi: 10.1016/0003-2697(79)90738-3.
   PubMed Web of Science ®Google Scholar
• Sedik AA, Elgohary R. Neuroprotective effect of tangeretin against chromium-induced acute brain injury in rats: targeting Nrf2 signaling pathway, inflammatory mediators, and apoptosis. Inflammopharmacology. 2023;31(3):1465–1480. doi: 10.1007/s10787-023-01167-3.
   PubMed Web of Science ®Google Scholar
• Sedik AA, Salama M, Fathy K, et al. Cold plasma approach fortifies the topical application of thymoquinone intended for wound healing via up-regulating the levels of TGF-ß, VEGF, and α-SMA in rats. Int Immunopharmacol. 2023;122:110634. doi: 10.1016/j.intimp.2023.110634.
   PubMed Web of Science ®Google Scholar
• Drury RAB, Wallington EA. Carleton’s histological technique. 5th ed. New York: Churchill Livingstone; 1980.
   Google Scholar
• Lai W-F, Shum HC. Hypromellose-graft-chitosan and its polyelectrolyte complex as novel systems for sustained drug delivery. ACS Appl Mater Interfaces. 2015;7(19):10501–10510. doi: 10.1021/acsami.5b01984.
   PubMed Web of Science ®Google Scholar
• Luckanagul JA, Pitakchatwong C, Bhuket PRN, et al. Chitosan-based polymer hybrids for thermo-responsive nanogel delivery of curcumin. Carbohydr Polym. 2018;181:1119–1127. doi: 10.1016/j.carbpol.2017.11.027.
   PubMed Web of Science ®Google Scholar
• Nawaz A, Ullah S, Alnuwaiser MA, et al. Formulation and evaluation of chitosan-gelatin thermosensitive hydrogels containing 5FU-alginate nanoparticles for skin delivery. Gels. 2022;8(9):537. doi: 10.3390/gels8090537.
   PubMed Web of Science ®Google Scholar
• Kufleitner J, Wagner S, Worek F, et al. Adsorption of obidoxime onto human serum albumin nanoparticles: drug loading, particle size and drug release. J Microencapsul. 2010;27(6):506–513. doi: 10.3109/02652041003681406.
   PubMed Web of Science ®Google Scholar
• Valencia MS, Silva Júnior M, Xavier-Júnior FH, et al. Characterization of curcumin-loaded lecithin-chitosan bioactive nanoparticles. Carbohydr Polym Technol Appl. 2021;2:100119. doi: 10.1016/j.carpta.2021.100119.
   Google Scholar
• Ren G, He Y, Liu C, et al. Encapsulation of curcumin in ZEIN–HTCC complexes: physicochemical characterization, in vitro sustained release behavior and encapsulation mechanism. LWT. 2022;155:112909. doi: 10.1016/j.lwt.2021.112909.
   Web of Science ®Google Scholar
• Sankalia MG, Mashru RC, Sankalia JM, et al. Reversed ­chitosan–alginate polyelectrolyte complex for stability ­improvement of alpha-amylase: optimization and physicochemical characterization. Eur J Pharm Biopharm. 2007;65(2):215–232. doi: 10.1016/j.ejpb.2006.07.014.
   PubMed Web of Science ®Google Scholar
• Jonassen H, Kjøniksen A-L, Hiorth M. Stability of chitosan nanoparticles cross-linked with tripolyphosphate. Biomacromolecules. 2012;13(11):3747–3756. doi: 10.1021/bm301207a.
   PubMed Web of Science ®Google Scholar
• Birch NP, Schiffman JDJL. Characterization of self-assembled polyelectrolyte complex nanoparticles formed from chitosan and pectin. Langmuir. 2014;30(12):3441–3447. doi: 10.1021/la500491c.
   PubMed Web of Science ®Google Scholar
• Sankhla A, Sharma R, Yadav RS, et al. Biosynthesis and characterization of cadmium sulfide nanoparticles – an emphasis of zeta potential behavior due to capping. Mater Chem. 2016;170:44–51.
   Web of Science ®Google Scholar
• Santo VE, Duarte ARC, Gomes ME, et al. Hybrid 3D structure of poly (d, l-lactic acid) loaded with chitosan/chondroitin sulfate nanoparticles to be used as carriers for biomacromolecules in tissue engineering. J Supercrit Fluids. 2010;54(3):320–327. doi: 10.1016/j.supflu.2010.05.021.
   Web of Science ®Google Scholar
• Omer AM, Ziora ZM, Tamer TM, et al. Formulation of quaternized aminated chitosan nanoparticles for efficient encapsulation and slow release of curcumin. Molecules. 2021;26(2):449. doi: 10.3390/molecules26020449.
   PubMed Web of Science ®Google Scholar
• Shah MKA, Azad AK, Nawaz A, et al. Formulation development, characterization and antifungal evaluation of chitosan NPs for topical delivery of voriconazole in vitro and ex vivo. Polymers. 2021;14(1):135. doi: 10.3390/polym14010135.
   PubMed Web of Science ®Google Scholar
• Sandri G, Bonferoni MC, Ferrari F, et al. The role of particle size in drug release and absorption. In: Merkus H, Meesters G, editors. Particulate products. Particle technology series. Springer, Cham; 2014. p. 323–341. doi: 10.1007/978-3-319-00714-4_11
   Google Scholar
• Rajitha P, Gopinath D, Biswas R, et al. Chitosan nanoparticles in drug therapy of infectious and inflammatory diseases. Expert Opin Drug Deliv. 2016;13(8):1177–1194. doi: 10.1080/17425247.2016.1178232.
   PubMed Web of Science ®Google Scholar
• Malviya R, Tyagi A, Fuloria S, et al. Fabrication and characterization of chitosan–tamarind seed polysaccharide composite film for transdermal delivery of protein/peptide. Int J Nanomed. 2021;13(9):1531. doi: 10.3390/polym13091531.
   Google Scholar
• Mardhiah Adib Z, Ghanbarzadeh S, Kouhsoltani M, et al. The effect of particle size on the deposition of solid lipid nanoparticles in different skin layers: a histological study. Adv Pharm Bull. 2016;6(1):31–36. doi: 10.15171/apb.2016.06.
   PubMedGoogle Scholar
• Sharkawy A, Silva AM, Rodrigues F, et al. Pickering emulsions stabilized with chitosan/collagen peptides nanoparticles as green topical delivery vehicles for cannabidiol (CBD). Colloids Surf A. 2021;631:127677. doi: 10.1016/j.colsurfa.2021.127677.
   Google Scholar
• Jantarat C, Sirathanarun P, Ratanapongsai S, et al. Curcumin-hydroxypropyl-β-cyclodextrin inclusion complex preparation methods: effect of common solvent evaporation, freeze drying, and pH shift on solubility and stability of curcumin. Trop J Pharm Res. 2014;13(8):1215–1223. doi: 10.4314/tjpr.v13i8.4.
   Web of Science ®Google Scholar
• Ganan M, Carrascosa AV, Martínez-Rodríguez AJ. Antimicrobial activity of chitosan against Campylobacter spp. and other microorganisms and its mechanism of action. J Food Prot. 2009;72(8):1735–1738. doi: 10.4315/0362-028x-72.8.1735.
   PubMed Web of Science ®Google Scholar
• Xing Y, Wang X, Guo X, et al. Comparison of antimicrobial activity of chitosan nanoparticles against bacteria and fungi. Coatings. 2021;11(7):769. doi: 10.3390/coatings11070769.
   Web of Science ®Google Scholar
• Quan Z, Luo C, Zhu B, et al. Synthesis and antimicrobial activities of chitosan/polypropylene carbonate-based nanoparticles. RSC Adv. 2021;11(17):10121–10129. doi: 10.1039/d0ra09257f.
   PubMed Web of Science ®Google Scholar
• Padmavathi AR, Abinaya B, Pandian SKJB. Phenol, 2,4-bis(1,1-dimethylethyl) of marine bacterial origin inhibits quorum sensing mediated biofilm formation in the uropathogen Serratia marcescens. Biofouling. 2014;30(9):1111–1122. doi: 10.1080/08927014.2014.972386.
   PubMed Web of Science ®Google Scholar
• Mohamad OA, Li L, Ma J-B, et al. Evaluation of the antimicrobial activity of endophytic bacterial populations from Chinese traditional medicinal plant licorice and characterization of the bioactive secondary metabolites produced by Bacillus atrophaeus against Verticillium dahliae. Front Microbiol. 2018;9:924. doi: 10.3389/fmicb.2018.00924.
   PubMed Web of Science ®Google Scholar
• Sökmen M, Akram Khan M. The antioxidant activity of some curcuminoids and chalcones. Inflammopharmacology. 2016;24(2–3):81–86. doi: 10.1007/s10787-016-0264-5.
   PubMed Web of Science ®Google Scholar
• Rajalakshmi A, Krithiga N, Jayachitra A. Antioxidant activity of the chitosan extracted from shrimp exoskeleton. Middle East J Sci Res. 2013;16(10):1446–1451.
   Google Scholar
• Xie W, Xu P, Liu QJB. Antioxidant activity of water-soluble chitosan derivatives. Bioorg Med Chem Lett. 2001;11(13):1699–1701. doi: 10.1016/s0960-894x(01)00285-2.
   PubMed Web of Science ®Google Scholar
• Tsuda T, Watanabe M, Ohshima K, et al. Antioxidative components isolated from the seed of tamarind (Tamarindus indica L.). J Agric Food Chem. 1994;42(12):2671–2674. doi: 10.1021/jf00048a004.
   Web of Science ®Google Scholar
• Sookying S, Duangjai A, Saokaew S, et al. Botanical aspects, phytochemicals, and toxicity of Tamarindus indica leaf and a systematic review of antioxidant capacities of T. indica leaf extracts. Front Nutr. 2022;9:977015. doi: 10.3389/fnut.2022.977015.
   PubMed Web of Science ®Google Scholar
• Chen L, Deng H, Cui H, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2018;9(6):7204–7218. doi: 10.18632/oncotarget.23208.
   PubMedGoogle Scholar
• Bao P, Kodra A, Tomic-Canic M, et al. The role of vascular endothelial growth factor in wound healing. J Surg Res. 2009;153(2):347–358. doi: 10.1016/j.jss.2008.04.023.
   PubMed Web of Science ®Google Scholar
• Abraham NG, Kappas A. Pharmacological and clinical aspects of heme oxygenase. Pharmacol Rev. 2008;60(1):79–127. doi: 10.1124/pr.107.07104.
   PubMed Web of Science ®Google Scholar
• Hussein RA, Salama AA, El Naggar ME, et al. Medicinal impact of microalgae collected from high rate algal ponds; phytochemical and pharmacological studies of microalgae and its application in medicated bandages. Biocatal Agric Biotechnol. 2019;20:101237. doi: 10.1016/j.bcab.2019.101237.
   Web of Science ®Google Scholar
• Abdel-Hakeem MA, Mongy S, Hassan B, et al. Curcumin loaded chitosan–protamine nanoparticles revealed antitumor activity via suppression of NF-κB, proinflammatory cytokines and bcl-2 gene expression in the breast cancer cells. J Pharm Sci. 2021;110(9):3298–3305. doi: 10.1016/j.xphs.2021.06.004.
   PubMed Web of Science ®Google Scholar
• Dunphy JE, Udupa K. Chemical and histochemical sequences in the normal healing of wounds. N Engl J Med. 1955;253(20):847–851. doi: 10.1056/NEJM195511172532002.
   PubMed Web of Science ®Google Scholar
• Haukipuro K. Synthesis of collagen types I and III in reincised wounds in humans. Br J Surg. 1991;78(6):708–712. doi: 10.1002/bjs.1800780624.
   PubMed Web of Science ®Google Scholar
• Al Bayaty F, Abdulla M, Abu Hassan M, et al. Wound healing potential by hyaluronate gel in streptozotocin-induced diabetic rats. Sci Res Essays. 2010;5(18):2756–2760.
   Google Scholar
• Hussain Z, Thu HE, Katas H, et al. Hyaluronic acid-based biomaterials: a versatile and smart approach to tissue regeneration and treating traumatic, surgical, and chronic wounds. Polym Rev. 2017;57(4):594–630. doi: 10.1080/15583724.2017.1315433.
   Web of Science ®Google Scholar
• Lopes GC, Sanches ACC, Nakamura CV, et al. Influence of extracts of Stryphnodendron polyphyllum Mart. and Stryphnodendron obovatum Benth. on the cicatrisation of cutaneous wounds in rats. J Ethnopharmacol. 2005;99(2):265–272. doi: 10.1016/j.jep.2005.02.019.
   PubMed Web of Science ®Google Scholar
• Mohanty C, Das M, Sahoo SK. Sustained wound healing activity of curcumin loaded oleic acid based polymeric bandage in a rat model. Mol Pharm. 2012;9(10):2801–2811. doi: 10.1021/mp300075u.
   PubMedGoogle Scholar
• Sumitra M, Manikandan P, Suguna L. Efficacy of Butea monosperma on dermal wound healing in rats. Int J Biochem Cell Biol. 2005;37(3):566–573. doi: 10.1016/j.biocel.2004.08.003.
   PubMed Web of Science ®Google Scholar
• Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv. 2015;5(35):27986–28006. doi: 10.1039/C4RA13315C.
   Web of Science ®Google Scholar
• de Zwart LL, Meerman JHN, Commandeur JNM, et al. Biomarkers of free radical damage: applications in ­experimental animals and in humans. Free Radic Biol Med. 1999;26(1–2):202–226. doi: 10.1016/s0891-5849(98)00196-8.
   PubMed Web of Science ®Google Scholar
• Sarawi WS, Alhusaini AM, Fadda LM, et al. Nano-curcumin prevents cardiac injury, oxidative stress and inflammation, and modulates TLR4/NF-κB and MAPK signaling in copper sulfate-intoxicated rats. Antioxidants. 2021;10(9):1414. doi: 10.3390/antiox10091414.
   Web of Science ®Google Scholar
• Shahbandeh M, Salehi MA, Soltanyzadeh M, et al. Evaluation of the antibacterial and wound healing properties of a burn ointment containing curcumin, honey, and potassium aluminium. arXiv preprint arXiv:11939; 2022.
   Google Scholar
• Panchatcharam M, Miriyala S, Gayathri VS, et al. Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species. Mol Cell Biochem. 2006;290(1–2):87–96. doi: 10.1007/s11010-006-9170-2.
   PubMed Web of Science ®Google Scholar
• Yen Y-H, Pu C-M, Liu C-W, et al. Curcumin accelerates cutaneous wound healing via multiple biological actions: the involvement of TNF-α, MMP-9, α-SMA, and collagen. Int Wound J. 2018;15(4):605–617. doi: 10.1111/iwj.12904.
   PubMed Web of Science ®Google Scholar