Biosafety and biosecurity in Synthetic Biology: A review

References

• Adamala, K., Martin-Alarcon, D., Guthrie-Honea, K., & Boyden, E. (2017). Engineering genetic circuit interactions within and between synthetic minimal cells. Nature Chemistry, 9(5), 431–439. doi:10.1038/nchem.2644
   PubMed Web of Science ®Google Scholar
• Ahteensuu, M. (2017). Synthetic biology, genome editing, and the risk of bioterrorism. Science and Engineering Ethics, 23(6), 1541–1561.
   PubMed Web of Science ®Google Scholar
• Altamura, E., Milano, F., Tangorra, R., Trotta, M., Omar, O., Stano, P., & Mavelli, F. (2017). Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells. Proceedings of the National Academy of Sciences, 114(15), 3837–3842. doi:10.1073/pnas.1617593114
   PubMed Web of Science ®Google Scholar
• Anderson, J., Wu, N., Santoro, S., Lakshman, V., King, D., & Schultz, P. (2004). An expanded genetic code with a functional quadruplet codon. Proceedings of the National Academy of Sciences of the United States of America, 101(20), 7566–7571.
   PubMed Web of Science ®Google Scholar
• Annaluru, N., Muller, H., Mitchell, L. A., Ramalingam, S., Stracquadanio, G., Richardson, S. M., … Chandrasegaran, S. (2014). Total synthesis of a functional designer eukaryotic chromosome. Science, 344(6179), 55–58.
   PubMed Web of Science ®Google Scholar
• Australia Group (AG). (2017). Statement by Australia Group Participants on the 20th anniversary of the entry into force of the Chemical Weapons Convention. Retrieved from http://www.australiagroup.net/en/cwc-20th-anniversary.html
   Google Scholar
• Bedau, M., Parke, E., Tangen, U., & Hantsche-Tangen, B. (2009). Social and ethical checkpoints for bottom-up synthetic biology, or protocells. Systems and Synthetic Biology, 3(1–4), 65–75.
   PubMedGoogle Scholar
• Benner, S. (2004). Understanding nucleic acids using synthetic chemistry. Accounts of Chemical Research, 37(10), 784–797. doi:10.1021/ar040004z
   PubMed Web of Science ®Google Scholar
• Benner, S., & Sismour, A. (2005). Synthetic biology. Nature Reviews Genetics, 6(7), 533–543.
   PubMed Web of Science ®Google Scholar
• Bollaert, C., & Whitby, S. (2012). Online applied dual-use biosecurity education: A case study from the University of Bradford. Medicine, Conflict, and Survival, 28(1), 59–71.
   PubMedGoogle Scholar
• Bügl, H., Danner, J. P., Molinari, R. J., Mulligan, J. T., Park, H.-O., Reichert, B., … Endy, D. (2007). DNA synthesis and biological security. Nature Biotechnology, 25(6), 627–629.
   PubMed Web of Science ®Google Scholar
• Cello, J., Paul, A., & Wimmer, E. (2002). Chemical synthesis of poliovirus cDNA: Generation of infectious virus in the absence of natural template. Science (New York, N.Y.), 297(5583), 1016–1018.
   PubMed Web of Science ®Google Scholar
• Chan, C., Lee, J., Cameron, D., Bashor, C., & Collins, J. (2016). ‘Deadman’ and ‘passcode’ microbial kill switches for bacterial containment. Nature Chemical Biology, 12(2), 82–86.
   PubMed Web of Science ®Google Scholar
• Chin, J., Cropp, T., Anderson, J., Mukherji, M., Zhang, Z., & Schultz, P. (2003). An expanded eukaryotic genetic code. Science (New York, N.Y.), 301(5635), 964–967.
   PubMed Web of Science ®Google Scholar
• Collins, F. (2012). Statement by NIH Director Francis Collins, M.D., Ph.D. on the NSABB review of revised H5N1 manuscripts. Retrieved from https://www.nih.gov/about-nih/who-we-are/nih-director/statements/statement-nih-director-francis-collins-md-phd-nsabb-review-revised-h5n1-manuscripts
   Google Scholar
• Colussi, I. (2013). Synthetic biology between challenges and risks: Suggestions for a model of governance and a regulatory framework, based on fundamental rights. Revista de Derecho y Genoma Humano, 38, 185–214.
   Google Scholar
• Colussi, I. (2015). Synthetic biology as a new threat to biosecurity. Is there a road to suitable governance? In C. Romeo Casabona (Ed.), Bioterrorismo y bioseguridad (pp. 65–110). Bizkaia, Spain: Cátedra Interuniversitaria Fundación BBV-Diputación Foral de Bizkaia de Derecho y Genoma Humano.
   Google Scholar
• D’Aguanno, E., Altamura, E., Mavelli, F., Fahr, A., Stano, P., & Luisi, P. (2015). Physical routes to primitive cells: An experimental model based on the spontaneous entrapment of enzymes inside micrometer-sized liposomes. Life (Basel), 5(1), 969–996. doi:10.3390/life5010969
   PubMedGoogle Scholar
• Darvishi, F., Ariana, M., Marella, E., & Borodina, I. (2018). Advances in synthetic biology of oleaginous yeast Yarrowia lipolytica for producing non-native chemicals. Applied Microbiology and Biotechnology, 102(14), 5925–5938. doi:10.1007/s00253-018-9099-x
   PubMed Web of Science ®Google Scholar
• de Lorenzo, V. (2010). Environmental biosafety in the age of synthetic biology: Do we really need a radical new approach? Environmental fates of microorganisms bearing synthetic genomes could be predicted from previous data on traditionally engineered bacteria for in situ bioremediation Bioessays: News and Reviews in Molecular, Cellular and Developmental Biology, 32(11), 926–931.
   PubMedGoogle Scholar
• Decoene, T., De Paepe, B., Maertens, J., Coussement, P., Peters, G., De Maeseneire, S., & De Mey, M. (2018). Standardization in synthetic biology: An engineering discipline coming of age. Critical Reviews in Biotechnology, 38(5), 647–656.
   PubMed Web of Science ®Google Scholar
• Deplazes, A. (2009). Piecing together a puzzle. An exposition of synthetic biology. EMBO Reports, 10(5), 428–432.
   PubMed Web of Science ®Google Scholar
• DiEuliis, D., & Gronvall, G. (2018). A holistic assessment of the risks and benefits of the synthesis of horsepox virus. mSphere, 3(2), e00074–e00018.
   PubMed Web of Science ®Google Scholar
• DiEuliis, D., Carter, S., & Gronvall, G. (2017). Options for synthetic DNA order screening, revisited. mSphere, 2(4), e00319–e00317.
   PubMed Web of Science ®Google Scholar
• Ding, Y., Contreras-Llano, L. E., Morris, E., Mao, M., & Tan, C. (2018). Minimizing context dependency of gene networks using artificial cells. ACS Applied Materials & Interfaces, 10(36), 30137–30146. doi:10.1021/acsami.8b10029
   PubMed Web of Science ®Google Scholar
• Douglas, T., & Savulescu, J. (2010). Synthetic biology and the ethics of knowledge. Journal of Medical Ethics, 36(11), 687–693.
   PubMed Web of Science ®Google Scholar
• Edwards, B. (2014). Taking stock of security concerns related to synthetic biology in an age of responsible innovation. Front Public Health, 2, 79.
   PubMedGoogle Scholar
• Edwards, B., & Kelle, A. (2012). A life scientist, an engineer and a social scientist walk into a lab: Challenges of dual-use engagement and education in synthetic biology. Medicine, Conflict, and Survival, 28(1), 5–18.
   PubMedGoogle Scholar
• Endy, D. (2005). Foundations for engineering biology. Nature, 438(7067), 449–453.
   PubMed Web of Science ®Google Scholar
• Evans, A. (2017). Glowing plants: Natural lighting with no electricity. Retrieved from https://www.kickstarter.com/projects/antonyevans/glowing-plants-natural-lighting-with-no-electricit/description
   Google Scholar
• Evans, N. (2014). Dual-use decision making: Relational and positional issues. Monash Bioethics Review, 32(3–4), 268–283.
   PubMedGoogle Scholar
• Evans, N., & Selgelid, M. (2015). Biosecurity and open-source biology: The promise and peril of distributed synthetic biological technologies. Science and Engineering Ethics, 21(4), 1065–1083. doi:10.1007/s11948-014-9591-3
   PubMed Web of Science ®Google Scholar
• Federal Select Agent Program (FSAP). (2017). Select agents and toxins list. Retrieved from https://www.selectagents.gov/SelectAgentsandToxinsList.html
   Google Scholar
• Fischer, M., & Maurer, S. (2010). Harmonizing biosecurity oversight for gene synthesis. Nature Biotechnology, 28(1), 20–22.
   PubMed Web of Science ®Google Scholar
• Flores Bueso, Y., Lehouritis, P., & Tangney, M. (2018). In situ biomolecule production by bacteria; a synthetic biology approach to medicine. Journal of Controlled Release, 275, 217–228. doi:10.1016/j.jconrel.2018.02.023
   PubMed Web of Science ®Google Scholar
• Friends of the Earth, CTA, & ETC Group. (2012). The principles for the oversight of synthetic biology. Retrieved from http://www.etcgroup.org/content/principles-oversight-synthetic-biology
   Google Scholar
• Garfinkel, M., Endy, D., Epstein, G., & Friedman, R. (2007). Synthetic genomics | Options for governance. Retrieved from http://www.synbiosafe.eu/uploads/pdf/Synthetic%20Genomics%20Options%20for%20Governance.pdf
   Google Scholar
• Geva, P., Kahta, R., Nakonechny, F., Aronov, S., & Nisnevitch, M. (2016). Increased copper bioremediation ability of new transgenic and adapted Saccharomyces cerevisiae strains. Environmental Science and Pollution Research, 23(19), 19613–19625. doi:10.1007/s11356-016-7157-4
   PubMed Web of Science ®Google Scholar
• Gibson, D. G., Glass, J. I., Lartigue, C., Noskov, V. N., Chuang, R.-Y., Algire, M. A., … Venter, J. C. (2010). Creation of a bacterial cell controlled by a chemically synthesized genome. Science, 329(5987), 52–56.
   PubMed Web of Science ®Google Scholar
• Herfst, S., Schrauwen, E. J. A., Linster, M., Chutinimitkul, S., de Wit, E., Munster, V. J., … Fouchier, R. A. M. (2012). Airborne transmission of influenza A/H5N1 virus between ferrets. Science, 336(6088), 1534–1541. doi:10.1126/science.1213362
   PubMed Web of Science ®Google Scholar
• Howard, J., Murashov, V., & Schulte, P. (2017). Synthetic biology and occupational risk. Journal of Occupational and Environmental Hygiene, 14(3), 224–236.
   PubMed Web of Science ®Google Scholar
• Hutchison, C. A., Chuang, R.-Y., Noskov, V. N., Assad-Garcia, N., Deerinck, T. J., Ellisman, M. H., … Venter, J. C. (2016). Design and synthesis of a minimal bacterial genome. Science (New York, N.Y.), 351(6280), aad6253.
   PubMed Web of Science ®Google Scholar
• Hwang, I. Y., Koh, E., Wong, A., March, J. C., Bentley, W. E., Lee, Y. S., & Chang, M. W. (2017). Engineered probiotic Escherichia coli can eliminate and prevent Pseudomonas aeruginosa gut infection in animal models. Nature Communications, 8, 15028. doi:10.1038/ncomms15028
   PubMed Web of Science ®Google Scholar
• Imai, M., Watanabe, T., Hatta, M., Das, S. C., Ozawa, M., Shinya, K., … Kawaoka, Y. &. (2012). Experimental adaptation of an influenza H5 HA confers respiratory droplet transmission to a reassortant H5 HA/H1N1 virus in ferrets. Nature, 486(7403), 420–428. doi:10.1038/nature10831
   PubMed Web of Science ®Google Scholar
• Imperiale, M. (2018). Re-creation of Horsepox Virus. mSphere, 3(2), e00079-18.
   PubMed Web of Science ®Google Scholar
• International Association Synthetic Biology (IASB). (2009). The IASB code of conduct for best practices in gene dynthesis. Retrieved from http://op.bna.com.s3.amazonaws.com/hl.nsf/r%3FOpen%3djaqo-7xqpnr
   Google Scholar
• International Gene Synthesis Consortium (IGSC). (2009). Harmonized screening protocol—Gene sequence & customer screening to promote biosecurity. Retrieved from http://www.genesynthesisconsortium.org/images/pdf/IGSC%20Harmonized%20Screening%20Protocol-11_18_09.pdf
   Google Scholar
• Isabella, V. M., Ha, B. N., Castillo, M. J., Lubkowicz, D. J., Rowe, S. E., Millet, Y. A., … Falb, D. (2018). Development of a synthetic live bacterial therapeutic for the human metabolic disease phenylketonuria. Nature Biotechnology, 36(9), 857–864. doi:10.1038/nbt.4222
   PubMed Web of Science ®Google Scholar
• Ishikawa, K., Sato, K., Shima, Y., Urabe, I., & Yomo, T. (2004). Expression of a cascading genetic network within liposomes. FEBS Letters, 576(3), 387–390.
   PubMed Web of Science ®Google Scholar
• Ito-Harashima, S., Mizutani, Y., Nishimura, M., Kim, H. J., Kim, Y. J., Kim, H. S., … Yagi, T. (2017). A pilot study for construction of a new cadmium-sensing yeast strain carrying a reporter plasmid with the JLP1 promoter. The Journal of Toxicological Sciences, 42(1), 103–109. doi:10.2131/jts.42.103
   PubMed Web of Science ®Google Scholar
• Jackson, R., Ramsay, A., Christensen, C., Beaton, S., Hall, D., & Ramshaw, I. (2001). Expression of mouse interleukin-4 by a recombinant ectromelia virus suppresses cytolytic lymphocyte responses and overcomes genetic resistance to mousepox. Journal of Virology, 75(3), 1205–1210. doi:10.1128/JVI.75.3.1205-1210.2001
   PubMed Web of Science ®Google Scholar
• Jagadevan, S., Banerjee, A., Banerjee, C., Guria, C., Tiwari, R., Baweja, M., & Shukla, P. (2018). Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production. Biotechnology for Biofuels, 11, 185.
   PubMed Web of Science ®Google Scholar
• Jefferson, C., Lentzos, F., & Marris, C. (2014). Synthetic biology and biosecurity: Challenging the “myths.” Frontiers in Public Health, 2(115), 115
   PubMedGoogle Scholar
• Kaebnick, G. (2010). New standards for gene synthesis screening. The Hastings Center Report, 40(1), 49
   Web of Science ®Google Scholar
• Kelle, A. (2007). Synthetic biology and biosecurity awareness in Europe. Retrieved from http://www.synbiosafe.eu/uploads///pdf/Synbiosafe-Biosecurity_awareness_in_Europe_Kelle.pdf
   Google Scholar
• Kelle, A. (2009a). Synthetic biology and biosecurity. From low levels of awareness to a comprehensive strategy. EMBO Reports, 10, S23–S27. doi:10.1038/embor.2009.119
   PubMed Web of Science ®Google Scholar
• Kelle, A. (2009b). Ensuring the security of synthetic biology-towards a 5P governance strategy. Systems and Synthetic Biology, 3(1–4), 85–90.
   PubMedGoogle Scholar
• Khalil, S., & Collins, J. (2010). Synthetic biology: Applications come of age. Nature Reviews Genetics, 11, 367–379.
   PubMed Web of Science ®Google Scholar
• Koblentz, G. (2017). The de novo synthesis of horsepox virus: Implications for biosecurity and recommendations for preventing the reemergence of smallpox. Health Security, 15(6), 620–628.
   PubMed Web of Science ®Google Scholar
• Koblentz, G. (2018). A critical analysis of the scientific and commercial rationales for the de novo synthesis of horsepox virus. mSphere, 3(2), e00040-18.
   PubMed Web of Science ®Google Scholar
• Krinsky, N., Kaduri, M., Zinger, A., Shainsky-Roitman, J., Goldfeder, M., Benhar, I., et al. (2018). Synthetic Cells Synthesize Therapeutic Proteins inside Tumors. Adv Healthc Mater, 7(9), e1701163.
   Google Scholar
• Kuhlau, F., Höglund, A., Eriksson, S., & Evers, K. (2013). The ethics of disseminating dual-use knowledge. Research Ethics, 9(1), 6–19. doi:10.1177/1747016113478517
   Google Scholar
• Kuiken, T. (2016). Governance: Learn from DIY biologists. Nature, 531(7593), 167–168.
   PubMed Web of Science ®Google Scholar
• Landrain, T., Meyer, M., Perez, A., & Sussan, R. (2013). Do-it-yourself biology: Challenges and promises for an open science and technology movement. Systems and Synthetic Biology, 7(3), 115–126.
   PubMedGoogle Scholar
• Ledford, H. (2012). Call to censor flu studies draws fire. Nature, 481(7379), 9–10.
   PubMed Web of Science ®Google Scholar
• Lee, J., Chan, C., Slomovic, S., & Collins, J. (2018). Next-generation biocontainment systems for engineered organisms. Nature Chemical Biology, 14, 530–537. doi:10.1038/s41589-018-0056-x
   PubMed Web of Science ®Google Scholar
• Lewis, D. D., Vanella, R., Vo, C. K., Rose, L., Nash, M., & Tan, C. (2018). Engineered stochastic adhesion between microbes as a protection mechanism against environmental stress. Cellular and Molecular Bioengineering, 11(5), 367–382. doi:10.1007/s12195-018-0552-9
   Web of Science ®Google Scholar
• Lyu, Y., Wu, C., Heinke, C., Han, D., Cai, R., Teng, I.-T., … Tan, W. (2018). Constructing smart protocells with built-in DNA computational core to eliminate exogenous challenge. Journal of the American Chemical Society, 140(22), 6912–6920. doi:10.1021/jacs.8b01960
   PubMed Web of Science ®Google Scholar
• MacIntyre, C. (2015). Biopreparedness in the age of genetically engineered pathogens and open access science: An urgent need for a paradigm shift. Military Medicine, 180(9), 943–949.
   PubMed Web of Science ®Google Scholar
• Mandell, D. J., Lajoie, M. J., Mee, M. T., Takeuchi, R., Kuznetsov, G., Norville, J. E., … Church, G. M. (2015). Biocontainment of genetically modified organisms by synthetic protein design. Nature, 518(7537), 55–60. doi:10.1038/nature14121
   PubMed Web of Science ®Google Scholar
• Maurer, S., Lucas, K., & Terrell, S. (2006). From understanding to action: Community-based options for improving safety and security in synthetic biology. Retrieved from https://pdfs.semanticscholar.org/b488/e96eebaf688a811112ad9de890ea2c08a1a1.pdf
   Google Scholar
• Miller, S., & Selgelid, M. (2007). Ethical and philosophical consideration of the dual-use dilemma in the Biological Sciences. Science and Engineering Ethics, 13(4), 523–580.
   PubMed Web of Science ®Google Scholar
• Mitchell, L., Wang, A., Stracquadanio, G., Kuang, Z., Wang, X., Yang, K., … Boeke, J. (2017). Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond. Science, 355(6329), eaaf4831. doi:10.1126/science.aaf4831
   PubMed Web of Science ®Google Scholar
• Moreno, E. (2012). Design and construction of “synthetic species.” PLoS One, 7(7), e39054.
   PubMed Web of Science ®Google Scholar
• National Academies of Sciences. (2018). Biodefense in the age of synthetic biology. Washington, DC: The National Academies Press. doi:10.17226/24890
   Google Scholar
• National Institutes of Health (NIH). (2011). Press statement on the NSABB review of H5N1 research. Retrieved from https://www.nih.gov/news-events/news-releases/press-statement-nsabb-review-h5n1-research
   Google Scholar
• National Institutes of Health (NIH). (2016). NIH guidelines for research involving recombinant or synthetic nucleic acid molecules. Retrieved from https://osp.od.nih.gov/wp-content/uploads/2013/06/NIH_Guidelines.pdf
   Google Scholar
• National Science Advisory Board for Biosecurity (NSABB). (2006). Addressing biosecurity concerns related to the synthesis of select agents. Retrieved from https://osp.od.nih.gov/wp-content/uploads/2013/06/Final_NSABB_Report_on_Synthetic_Genomics.pdf
   Google Scholar
• National Science Advisory Board for Biosecurity (NSABB). (2010). Addressing biosecurity concerns related to synthetic biology. Retrieved from https://osp.od.nih.gov/wp-content/uploads/NSABB_SynBio_DRAFT_Report-FINAL-2_6-7-10.pdf
   Google Scholar
• National Science Advisory Board for Biosecurity (NSABB). (2012). National Science Advisory Board for Biosecurity findings and recommendations. Retrieved from https://www.nih.gov/sites/default/files/about-nih/nih-director/statements/collins/03302012_NSABB_Recommendations.pdf
   Google Scholar
• Noireaux, V., & Libchaber, A. (2004). A vesicle bioreactor as a step toward an artificial cell assembly. Proceedings of the National Academy of Sciences of the United States of America, 101(51), 17669–17674.
   PubMed Web of Science ®Google Scholar
• Novossiolova, T., & Sture, J. (2012). Towards the responsible conduct of scientific research: Is ethics education enough? Medicine, Conflict and Survival, 28(1), 73–84. doi:10.1080/13623699.2012.658627
   PubMedGoogle Scholar
• Noyce, R., Lederman, S., & Evans, D. (2018). Construction of an infectious horsepox virus vaccine from chemically synthesized DNA fragments. PLoS One, 13(1), e0188453
   PubMed Web of Science ®Google Scholar
• Oye, K., Lawson, J., & Bubela, T. (2015). Drugs: Regulate ‘home-brew’ opiates. Nature, 521(7552), 281–283.
   PubMed Web of Science ®Google Scholar
• Palmer, M., Fukuyama, F., & Relman, D. (2015). Science governance. A more systematic approach to biological risk. Science (New York, N.Y.), 350(6267), 1471–1473.
   PubMed Web of Science ®Google Scholar
• Patterson, M. (2010). A Biopunk Manifesto. Retrieved from http://maradydd.livejournal.com/496085.html
   Google Scholar
• Presidential Commission for the Study of Bioethical Issues (PCSBI). (2010). New directions. The ethics of synthetic biology and emerging technologies. Washington, DC: PCSBI.
   Google Scholar
• Race, M., & Hammond, E. (2008). An evaluation of the role and effectiveness of institutional biosafety committees in providing oversight and security at biocontainment laboratories. Biosecurity and Bioterrorism: Biodefense Strategy, Practice, and Science, 6(1), 19–35. doi:10.1089/bsp.2007.0048
   PubMedGoogle Scholar
• Rasmussen, S., Constantinescu, A., & Svaneborg, C. (2016). Generating minimal living systems from non-living materials and increasing their evolutionary abilities. Philosophical Transactions of the Royal Society B: Biological Sciences, 371(1701), 20150440. doi:10.1098/rstb.2015.0440
   PubMed Web of Science ®Google Scholar
• Resnik, D. (2013). H5N1 avian flu research and the ethics of knowledge. The Hastings Center Report, 43(2), 22–33.
   PubMed Web of Science ®Google Scholar
• Revill, J., Carnevali, M. D. C., Forsberg, Å., Holmström, A., Rath, J., Shinwari, Z. K., & Mancini, G. M. (2012). Lessons learned from implementing education on dual-use in Austria, Italy, Pakistan and Sweden. Medicine, Conflict and Survival, 28(1), 31–44. doi:10.1080/13623699.2012.658624
   PubMedGoogle Scholar
• Rovner, A. J., Haimovich, A. D., Katz, S. R., Li, Z., Grome, M. W., Gassaway, B. M., … Isaacs, F. J. (2015). Recoded organisms engineered to depend on synthetic amino acids. Nature, 518(7537), 89–93. doi:10.1038/nature14095
   PubMed Web of Science ®Google Scholar
• Roy, L. (2017). FDA grants fast track designation to Synlogic’s SYNB1020 synthetic biotic treatment. Retrieved from https://www.pharmaceutical-technology.com/news/newsfda-grants-fast-track-designation-to-synlogics-synb1020-synthetic-biotic-treatment-5853672/
   Google Scholar
• Samuel, G., Selgelid, M., & Kerridge, I. (2009). Managing the unimaginable. Regulatory responses to the challenges posed by synthetic biology and synthetic genomics. EMBO Reports, 10(1), 7–12. doi:10.1038/embor.2008.232
   PubMed Web of Science ®Google Scholar
• Schmidt, M. (2008). Diffusion of synthetic biology: A challenge to biosafety. Systems and Synthetic Biology, 2(1-2), 1–6.
   PubMedGoogle Scholar
• Schmidt, M. (2009). Do I understand what I can create? Biosafety issues in synthetic biology. In M. Schmidt, A. Kelle, A. Ganguli-Mitra, & H. d. Vriend (Eds.), Synthetic biology. The technoscience and its societal consequences. Berlin, Germany: Springer.
   Google Scholar
• Schmidt, M., & de Lorenzo, V. (2016). Synthetic bugs on the loose: Containment options for deeply engineered (micro)organisms. Current Opinion in Biotechnology, 38, 90–96. doi:10.1016/j.copbio.2016.01.006
   PubMed Web of Science ®Google Scholar
• Schmidt, M., Ganguli-Mitra, A., Torgersen, H., Kelle, A., Deplazes, A., & Biller-Andorno, N. (2009). A priority paper for the societal and ethical aspects of synthetic biology. Systems and Synthetic Biology, 3(1-4), 3–7. doi:10.1007/s11693-009-9034-7
   PubMedGoogle Scholar
• Schmidt, M., Torgersen, H., Ganguli-Mitra, A., Kelle, A., Deplazes, A., & Biller-Andorno, N. (2008). SYNBIOSAFE e-conference: Online community discussion on the societal aspects of synthetic biology. Systems and Synthetic Biology, 2(1-2), 7–17. doi:10.1007/s11693-008-9019-y
   PubMedGoogle Scholar
• Schwille, P., Spatz, J., Landfester, K., Bodenschatz, E., Herminghaus, S., & Sourjik, V. (2018). MaxSynBio: Avenues towards creating cells from the bottom up. Angewandte Chemie International Edition in English, 57, 13382–13392. doi:10.1002/anie.201802288
   PubMed Web of Science ®Google Scholar
• Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR), Scientific Committee on Consumer Safety (SCCS), & Scientific Committee on Health and Environmental Risks (SCHER). (2014). Opinion on synthetic biology I: Definition. Retrieved from http://ec.europa.eu/health/scientific_committees/emerging/docs/scenihr_o_044.pdf
   Google Scholar
• Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR), Scientific Committee on Health and Environmental Risks (SCHER), & Scientific Committee on Consumer Safety (SCCS). (2015a). Opinion on synthetic biology II: Risk assessment methodologies and safety aspects. Retrieved from https://ec.europa.eu/health/sites/health/files/scientific_committees/emerging/docs/scenihr_o_048.pdf
   Google Scholar
• Scientific Committee on Emerging and Newly Identified Health Risks (SCENIHR), Scientific Committee on Health and Environmental Risks (SCHER), & Scientific Committee on Consumer Safety (SCCS). (2015b). Final opinion on synthetic biology III: Risks to the environment and biodiversity related to synthetic biology and research priorities in the field of synthetic biology. Retrieved from http://ec.europa.eu/health/scientific_committees/emerging/docs/scenihr_o_050.pdf
   Google Scholar
• Selgelid, M. (2007). A tale of two studies; ethics, bioterrorism, and the censorship of science. The Hastings Center Report, 37(3), 35–43.
   PubMed Web of Science ®Google Scholar
• Seyfried, G., Pei, L., & Schmidt, M. (2014). European do-it-yourself (DIY) biology: Beyond the hope, hype and horror. Bioessays: News and Reviews in Molecular, Cellular and Developmental Biology, 36(6), 548–551.
   PubMed Web of Science ®Google Scholar
• Sleator, R. (2016). Synthetic biology: From mainstream to counterculture. Archives of Microbiology, 198(7), 711–713.
   PubMed Web of Science ®Google Scholar
• Sture, J., & Whitby, S. (2012). Preventing the hostile use of the life sciences and biotechnologies; fostering a culture of biosecurity and dual use awareness. Conclusions. Medicine, Conflict and Survival, 28(1), 99–105.
   PubMedGoogle Scholar
• Sture, J., Minehata, M., & Shinomiya, N. (2012). Looking at the formulation of national biosecurity education action plans. Medicine, Conflict and Survival, 28(1), 85–97. doi:10.1080/13623699.2012.658628
   PubMedGoogle Scholar
• Sture, J., Whitby, S., & Perkins, D. (2013). Biosafety, biosecurity and internationally mandated regulatory regimes: Compliance mechanisms for education and global health security. Medicine, Conflict and Survival, 29(4), 289–321. doi:10.1080/13623699.2013.841355
   PubMedGoogle Scholar
• Török, T. J., Tauxe, R. V., Wise, R. P., Livengood, J. R., Sokolow, R., Mauvais, S., … Foster, L. R. (1997). A large community outbreak of salmonellosis caused by intentional contamination of restaurant salad bars. JAMA, 278(5), 389–395.
   PubMed Web of Science ®Google Scholar
• Torres, L., Krüger, A., Csibra, E., Gianni, E., & Pinheiro, V. (2016). Synthetic biology approaches to biological containment: Pre-emptively tackling potential risks. Essays in Biochemistry, 60(4), 393–410. doi:10.1042/EBC20160013
   PubMed Web of Science ®Google Scholar
• Tumpey, T. M., Basler, C. F., Aguilar, P. V., Zeng, H., Solórzano, A., Swayne, D. E., … García-Sastre, A. (2005). Characterization of the reconstructed 1918 Spanish influenza pandemic virus. Science (New York, N.Y.), 310(5745), 77–80.
   PubMed Web of Science ®Google Scholar
• United Nations Office for Disarmament Affairs (UNODA). (1972). Convention on the prohibition of the development, production and stockpiling of bacteriological (biological) and toxin weapons and on their destruction. Retrieved from http://disarmament.un.org/treaties/t/bwc/text
   Google Scholar
• US Department of Health and Human Services. (2010). Screening Framework Guidance for Providers of Synthetic Double-Stranded DNA. Retrieved from http://www.phe.gov/Preparedness/legal/guidance/syndna/Documents/syndna-guidance.pdf
   Google Scholar
• US Government. (2012). United States government policy for oversight of life sciences dual use research of concern. Retrieved from https://www.phe.gov/s3/dualuse/Documents/us-policy-durc-032812.pdf
   Google Scholar
• US Government. (2014). United States government policy for institutional oversight of life sciences dual use research of concern. Retrieved from https://www.phe.gov/s3/dualuse/Documents/durc-policy.pdf
   Google Scholar
• VERTIC. (2002). BWC legislation database. Retrieved from http://www.vertic.org/pages/homepage/programmes/national-implementation-measures/biological-weapons-and-materials/bwc-legislation-database/introduction.php
   Google Scholar
• Wang, L., Brock, A., Herberich, B., & Schultz, P. (2001). Expanding the genetic code of Escherichia coli. Science, 292(5516), 498–500.
   PubMed Web of Science ®Google Scholar
• Wolinsky, H. (2016). The FBI and biohackers: An unusual relationship. EMBO Reports, 17(6), 793–796.
   PubMed Web of Science ®Google Scholar
• World Health Organization (WHO). (2006). Biorisk management: Laboratory biosecurity guidance. Retrieved from http://www.who.int/ihr/publications/WHO_CDS_EPR_2006_6.pdf?ua=1
   Google Scholar
• Wright, O., Delmans, M., Stan, G., & Ellis, T. (2015). GeneGuard: A modular plasmid system designed for biosafety. ACS Synthetic Biology, 4(3), 307–316.
   PubMed Web of Science ®Google Scholar
• Wright, O., Stan, G., & Ellis, T. (2013). Building-in biosafety for synthetic biology. Microbiology (Reading, England)), 159(Pt 7), 1221–1235.
   PubMedGoogle Scholar
• Xie, J., & Schultz, P. (2006). A chemical toolkit for proteins—An expanded genetic code. Nature Reviews Molecular Cell Biology, 7(10), 775–782.
   PubMed Web of Science ®Google Scholar
• Xu, C., Hu, S., & Chen, X. (2016). Artificial cells: From basic science to applications. Mater Today (Kidlington), 19(9), 516–532. doi:10.1016/j.mattod.2016.02.020
   PubMedGoogle Scholar
• Zhang, Y., Ptacin, J. L., Fischer, E. C., Aerni, H. R., Caffaro, C. E., San Jose, K., … Romesberg, F. E. (2017). A semi-synthetic organism that stores and retrieves increased genetic information. Nature, 551(7682), 644–647. doi:10.1038/nature24659
   PubMed Web of Science ®Google Scholar