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Original Article

Ascorbic acid decreases oxidant stress in endothelial cells caused by the nitroxide tempol

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Pages 195-202 | Received 21 Oct 2004, Published online: 07 Jul 2009

References

  • Mitchell JB, DeGraff W, Kaufman D, Krishna MC, Samuni A, Finkelstein E, Ahn MS, Hahn SM, Gamson J, Russo A. Inhibition of oxygen-dependent radiation-induced damage by the nitroxide superoxide dismutase mimic, tempol. Arch Biochem Biophys 1991;289:62–70.
  • Samuni A, Winkelsberg D, Pinson A, Hahn SM, Mitchell JB, Russo A. Nitroxide stable radicals protect beating cardiomyocytes against oxidative damage. J Clin Investig 1991;87:1526–1530.
  • Mohsen M, Pinson A, Zhang R, Samuni A. Do nitroxides protect cardiomyocytes from hydrogen peroxide or super-oxide? Mol Cell Biochem 1995;145:103–110.
  • Beit-Yannai E, Zhang R, Trembovler V, Samuni A, Shohami E. Cerebroprotective effect of stable nitroxide radicals in closed head injury in the rat. Brain Res 1996;717:22–28.
  • Kroll C, Langner A, Borchert HH. Nitroxide metabolism in the human keratinocyte cell line HaCaT. Free Radic Biol Med 1999;26:850–857.
  • Krishna MC, Samuni A. Nitroxides as antioxidants. Methods Enzymol 1994;234:580–589.
  • Krishna MC, Russo A, Mitchell JB, Goldstein S, Dafni H, Samuni A. Do nitroxide antioxidants act as scavengers of 02. or as SOD mimics? J Biol Chem 1996;271:26026–26031.
  • Zollner S, Haseloff RF, Kirilyuk IA, Blasig IE, Rubanyi GM. Nitroxides increase the detectable amount of nitric oxide released from endothelial cells. J Biol Chem 1997;272:23076–23080.
  • Mitchell JB, Samuni A, Krishna MC, DeGraff WG, Ahn MS, Samuni U, Russo A. Biologically active metal-independent superoxide dismutase mimics. Biochemistry 1990; 29:2802–2807.
  • Gariboldi MB, Lucchi S, Caserini C, Supino R, Oliva C, Monti E. Antiproliferative effect of the piperidine nitroxide Tempol on neoplastic and nonneoplastic mammalian cell lines. Free Radic Biol Med 1998;24:913–923.
  • Gariboldi MB, Rimoldi V, Supino R, Favini E, Monti E. The nitroxide Tempol induces oxidative stress, p21wAFI/c/P1, and cell death in HL60 cells. Free Radic Biol Med 2000; 29:633–641.
  • Monti E, Supino R, Colleoni M, Costa B, Ravizza R, Gariboldi MB. Nitroxide Tempol impairs mitochondrial function and induces apoptosis in HL60 cells. J Cell Biochem 2001;82:271–276.
  • Samuni Y, Gamson J, Samuni A, Yamada K, Russo A, Krishna MC, Mitchell JB. Factors influencing nitroxide reduction and cytotoxicity in vitro. Antioxid. Redox Signal. 2004;6:587–595.
  • Ross AH, McConnell HM. Permeation of a spin-label phosphate into the human erythrocyte. Biochemistry 1975;14:2793–2798.
  • Branca M, Denurra T, Turrini F. Reduction of nitroxide free radical by normal and G6PD deficient red blood cells. Free Radic Biol Med 1988;5: 7–11.
  • Morrisett JD, Drott HR. Oxidation of the sulfhydryl and disulfide groups by the nitroxyl radical. J Biol Chem 1969;244:5083–5084.
  • Giotta GJ, Wang HH. Reduction of nitroxide free radicals by biological materials. Biochem Biophys Res Commun 1972;46:1576–1580.
  • Winkler BS, Orselli SM, Rex TS. The redox couple between glutathione and ascorbic acid: A chemical and physiological perspective. Free Radic Biol Med 1994;17:333–349.
  • May JM, Qu Z-C, Mendiratta S. Protection and recycling of ct-tocopherol in human erythrocytes by intracellular ascorbic acid. Arch Biochem Biophys 1998;349:281–289.
  • VanDuijn MM, Tijssen K, VanSteveninck J, van den Broek PJA, Van der Zee J. Erythrocytes reduce extracellular ascorbate free radicals using intracellular ascorbate as an electron donor. J Biol Chem 2000;275:27720–27725.
  • Mendiratta S, Qu Z-C, May JM. Erythrocyte ascorbate recycling: antioxidant effects in blood. Free Radic Biol Med 1998;24:789–797.
  • May JM, Qu Z-C, Li X. Ascorbic acid blunts oxidant stress due to menadione in endothelial cells. Arch Biochem Biophys 2003;411:136–144.
  • May JM, Qu Z-C, Li X. Nitrite generates an oxidant stress and increases nitric oxide in EA.hy926 endothelial cells. Free Radic Res 2004;38: 581–589.
  • 1Wartensson J, Meister A. Glutathione deficiency decreases tissue ascorbate levels in newborn rats: Ascorbate spares glutathione and protects. Proc Natl Acad Sci USA 1991;88: 4656–4660.
  • Smith AR, Visioli F, Hagen TM. Vitamin C matters: Increased oxidative stress in cultured human aortic endothelial cells without supplemental ascorbic acid. FASEB J 2002;16:1102–1104.
  • Fujiwara M, Nagao N, Monden K, Misumi M, Kageyama K, Yamamoto K, Miwa N. Enhanced protection against peroxidation-induced mortality of aortic endothelial cells by ascorbic acid-2-0-phosphate abundantly accumulated in the cell as the dephosphorylated form. Free Radic Res 1997;27: 97–104.
  • Ek A, Strom K, Cotgreave IA. The uptake of ascorbic acid into human umbilical vein endothelial cells and its effect on oxidant insult. Biochem Pharmacol 1995;50:1339–1346.
  • Ross R. Atherosclerosis-an inflammatory disease. N Engl J Med 1999;340:115–126.
  • Edgell CJ, McDonald CC, Graham JB. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc Natl Acad Sci USA 1983;80:3734–3737.
  • Bauer J, Margolis M, Schreiner C, Edgell CJ, Azizkhan J, Lazarowski E, Juliano RL. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: Contri-butions of induced gene expression, G-proteins, and integrins. J Cell Physiol 1992;153:437–449.
  • Pech-Amsellem MA, Myara I, Pico I, Maziere C, Maziere JC, Moatti N. Oxidative modifications of low-density lipoproteins (LDL) by the human endothelial cell line EA.hy 926. Experientia 1996;52: 234–238.
  • Avron M, Shavit N. A sensitive and simple method for determination of ferrocyanide. Anal Biochem 1963; 6:549–554.
  • Ross D, Mendiratta S, Qu ZC, Cobb CE, May JM. Ascorbate 6-palmitate protects human erythrocytes from oxidative damage. Free Radic Biol Med 1999;26: 81–89.
  • Hissin PJ, Hilf R. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 1976;74:214–226.
  • Jones W, Li X, Perriott LM, Whitesell RR, May JM. Uptake, recycling, and antioxidant functions of ek-lipoic acid in endothelial cells. Free Radic Biol Med 2002;33:83–93.
  • May JM, Qu Z-C. Redox regulation of ascorbic acid transport: Role of transporter and intracellular sulfhydryls. Biofactors 2004;8:1–13.
  • Deslauriers R, Butler K, Smith IC. Oxidant stress in malaria as probed by stable nitroxide radicals in erythrocytes infected with Plasmodium berghei. The effects of primaquine and chloroquine. Biochim Biophys Acta 1987; 931:267–275.
  • Orringer EP, Roer ME. An ascorbate-mediated transmem-brane-reducing system of the human erythrocyte. J Clin Investig 1979;63:53–58.
  • May JM, Qu Z-C, Whitesell RR. Ascorbate is the major electron donor for a transmembrane oxidoreductase of human erythrocytes. Biochim Biophys Acta 1995;1238:127–136.
  • Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM. Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-47'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123. Free Radic Biol Med 1999;27: 146–159.
  • Kalinich JF, Ramakrishnan N, McClain DE. The antioxidant Trolox enhances the oxidation of 2',7'-dichlorofluorescin to 2',7'-dichlorofluorescein. Free Radic Res 1997;26: 37–47.
  • May JM, Qu ZC, Li X. Requirement for GSH in recycling of ascorbic acid in endothelial cells. Biochem Pharmacol 2001;62:873–881.
  • Han D, Handelman G, Marcocci L, Sen CK, Roy S, Kobuchi H, Tritschler HJ, Flohe L, Packer L. Lipoic acid increases de now synthesis of cellular glutathione by improving cystine utilization. Biofactors 1997;6:321–338.
  • Rose RC. Transport of ascorbic acid and other water-soluble vitamins. Biochim Biophys Acta 1988;947:335–366.
  • Rice ME, Russo-Menna I. Differential compartmentalization of brain ascorbate and glutathione between neurons and glia. Neuroscience 1998;82:1213–1223.
  • May JM, Asard H. Ascorbate Recycling. In: Asard H, May JM, Smirnoff N, editors. Vitamin C. Functions and Biochemistry in Animals and Plants. London: Bios Scientific Publishers; 2004. p 139–158.

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