CONSTRUCTION AND EXPRESSION OF A SPLICED VARIANT OF EPSTEIN-BARR VIRUS BZLF1 AND PREPARATION OF ITS POLYCLONAL ANTIBODY

REFERENCES

• Bhende , P.M. ; Seaman , W.T. ; Delecluse , H.J. ; Kenney , S.C. The EBV lytic switch protein, Z, preferentially binds to and activates the methylated viral genome . Nat. Genet. 2004 , 36 ( 10 ), 1099 – 1104 .
   PubMed Web of Science ®Google Scholar
• Chang , Y.N. ; Dong , D.L. ; Hayward , G.S. ; Hayward , S.D. The Epstein-Barr virus Zta transactivator: a member of the bZIP family with unique DNA-binding specificity and a dimerization domain that lacks the characteristic heptad leucine zipper motif . J. Virol. 1990 , 64 ( 7 ), 3358 – 3369 .
   PubMed Web of Science ®Google Scholar
• Kenney , S. ; Holley-Guthrie , E. ; Mar , E.C. ; Smith , M. The Epstein-Barr virus BMLF1 promoter contains an enhancer element that is responsive to the BZLF1 and BRLF1 transactivators. J. Virol. 1989, 63 (9), 3878–3883.
   PubMed Web of Science ®Google Scholar
• Fixman , E.D. ; Hayward , G.S. ; Hayward , S.D. Trans-acting requirements for replication of Epstein-Barr virus ori-Lyt . J. Virol. 1992 , 66 ( 8 ), 5030 – 5039 .
   PubMed Web of Science ®Google Scholar
• Sinclair , A.J. ; Farrell , P.J. Epstein-Barr virus transcription factors . Cell Growth Differ. 1992 , 3 ( 8 ), 557 – 563 .
   PubMedGoogle Scholar
• Wu , F.Y. ; Chen , H. ; Wang , S.E. ; ApRhys , C.M. ; Liao , G. ; Fujimuro , M. ; Farrell , C.J. ; Huang , J. ; Hayward , S.D. ; Hayward , G.S. CCAAT/enhancer binding protein alpha interacts with ZTA and mediates ZTA-induced p21(CIP-1) accumulation and G(1) cell cycle arrest during the Epstein-Barr virus lytic cycle . J. Virol. 2003 , 77 ( 2 ), 1481 – 1500 .
   PubMed Web of Science ®Google Scholar
• Zerby , D. ; Chen , C.J. ; Poon , E. ; Lee , D. ; Shiekhattar , R. ; Lieberman , P.M. The amino-terminal C/H1 domain of CREB binding protein mediates zta transcriptional activation of latent Epstein-Barr virus . Mol. Cell Biol. 1999 , 19 ( 3 ), 1617 – 1626 .
   PubMed Web of Science ®Google Scholar
• Zhang , Q. ; Gutsch , D. ; Kenney , S. Functional and physical interaction between p53 and BZLF1: implications for Epstein-Barr virus, latency . Mol. Cell Biol. 1994 , 14 ( 3 ), 1929 – 1938 .
   PubMed Web of Science ®Google Scholar
• Speck , S.H. ; Chatila , T. ; Flemington , E. Reactivation of Epstein-Barr virus: regulation and function of the BZLF1 gene . Trends Microbiol. 1997 , 5 ( 10 ), 399 – 405 .
   PubMed Web of Science ®Google Scholar
• Packham , G. ; Economou , A. ; Rooney , C.M. ; Rowe , D.T. ; Farrell , P.J. Structure and function of the Epstein-Barr virus BZLF1 protein . J. Virol. 1990 , 64 ( 5 ), 2110 – 2116 .
   PubMed Web of Science ®Google Scholar
• Lieberman , P.M. ; Berk , A.J. In vitro transcriptional activation, dimerization, and DNA-binding specificity of the Epstein-Barr virus Zta protein . J. Virol. 1990 , 64 ( 6 ), 2560 – 2568 .
   PubMed Web of Science ®Google Scholar
• Schepers , A. ; Pich , D. ; Hammerschmidt , W. A transcription factor with homology to the AP-1 family links RNA transcription and DNA replication in the lytic cycle of Epstein-Barr virus . EMBO J. 1993 , 12 ( 10 ), 3921 – 3929 .
   PubMed Web of Science ®Google Scholar
• Farrell , P.J. ; Rowe , D.T. ; Rooney , C.M. ; Kouzarides , T. Epstein-Barr virus BZLF1 trans-activator specifically binds to a consensus AP-1 site and is related to c-fos . EMBO J. 1989 , 8 ( 1 ), 127 – 132 .
   PubMed Web of Science ®Google Scholar
• Flemington , E. ; Speck , S.H. Identification of phorbol ester response elements in the promoter of Epstein-Barr virus putative lytic switch gene BZLF1 . J. Virol. 1990 , 64 ( 3 ), 1217 – 1226 .
   PubMed Web of Science ®Google Scholar
• Francis , A.L. ; Gradoville , L. ; Miller , G. Alteration of a single serine in the basic domain of the Epstein-Barr virus ZEBRA protein separates its functions of transcriptional activation and disruption of latency . J. Virol. 1997 , 71 ( 4 ), 3054 – 3061 .
   PubMed Web of Science ®Google Scholar
• Bannister , Z.J. ; Cook , A. ; Kouzarides , T. In vivo DNA binding activity of Fos/Jun and BZLF1 but not C/EBP is affected by redox changes . Oncogene 1991 , 6 , 1243 – 1250 .
   PubMed Web of Science ®Google Scholar
• Kolman , J.L. ; Taylor , N. ; Marshak , D.R. ; Miller , G. Serine-173 of the Epstein-Barr virus ZEBRA protein is required for DNA binding and is a target for casein kinase II phosphorylation. Proc. Natl. Acad. Sci. U. S. A. 1993, 90 (21), 10115–10119.
   PubMed Web of Science ®Google Scholar
• Lau , R. ; Packham , G. ; Farrell , P.J. Differential splicing of Epstein-Barr virus immediate-early RNA . J. Virol. 1992 , 66 ( 10 ), 6233 – 6236 .
   PubMed Web of Science ®Google Scholar
• Huang , J. ; Chen , H. ; Hutt-Fletcher , L. ; Ambinder , R.F. ; Hayward , S.D. Lytic viral replication as a contributor to the detection of Epstein-Barr virus in breast cancer . J. Virol. 2003 , 77 ( 24 ), 13267 – 13274 .
   PubMed Web of Science ®Google Scholar
• Rooney , C.M. ; Rowe , D.T. ; Ragot , T. ; Farrell , P.J. The spliced BZLF1 gene of Epstein-Barr virus (EBV) transactivates an early EBV promoter and induces the virus productive cycle . J. Virol. 1989 , 63 ( 7 ), 3109 – 3116 .
   PubMed Web of Science ®Google Scholar
• Flemington , E.K. ; Lytle , J.P. ; Cayrol , C. ; Borras , A.M. ; Speck , S.H. DNA-binding- defective mutants of the Epstein-Barr virus lytic switch activator Zta transactivate with altered specificities . Mol. Cell Biol. 1994 , 14 ( 5 ), 3041 – 3052 .
   PubMed Web of Science ®Google Scholar
• Hicks , M.R. ; Al-Mehairi , S.S. ; Sinclair , A.J. The zipper region of Epstein-Bar virus bZIP transcription factor Zta is necessary but not sufficient to direct DNA binding . J. Virol. 2003 , 77 ( 14 ), 8173 – 8177 .
   PubMed Web of Science ®Google Scholar
• Carey , M. ; Kolman , J. ; Katz , D.A. ; Gradoville , L. ; Barberis , L. ; Miller , G. Transcriptional synergy by the Epstein-Barr virus transactivator ZEBRA . J. Virol. 1992 , 66 ( 8 ), 4803 – 4813 .
   PubMed Web of Science ®Google Scholar
• Morrison , T.E. ; Mauser , A. ; Klingelhutz , A. ; Kenney , S.C. Epstein-Barr virus immediate-early protein BZLF1 inhibits tumor necrosis factor alpha-induced signaling and apoptosis by downregulating tumor necrosis factor receptor 1 . J. Virol. 2004 , 78 ( 1 ), 544 – 549l .
   PubMed Web of Science ®Google Scholar