Development of an improved lentiviral based vector system for the stable expression of monoclonal antibody in CHO cells

References

• Kunert, R.; Reinhart, D. Advances in Recombinant Antibody Manufacturing. Appl. Microbiol. Biotechnol. 2016, 100, 3451–3461.
   PubMed Web of Science ®Google Scholar
• Deng, X.; Storz, U.; Doranz, B. J. Enhancing Antibody Patent Protection Using Epitope Mapping Information. MAbs 2018, 10, 204–209.
   PubMed Web of Science ®Google Scholar
• Walsh, G. Biopharmaceutical Benchmarks 2018. Nat. Biotechnol. 2018, 36, 1136–1145.
   PubMed Web of Science ®Google Scholar
• Shukla, A.A.; Wolfe, L.S.; Mostafa, S.S.; Norman, C. Evolving Trends in mAb Production Processes. Bioeng. Transl. Med. 2017, 2, 58–69.
   PubMed Web of Science ®Google Scholar
• Shepard, H.M.; Phillips, G.L.; D Thanos C.; Feldmann, M. Developments in Therapy with Monoclonal Antibodies and Related Proteins. Clin. Med. (Lond). 2017, 17, 220–232.
   PubMed Web of Science ®Google Scholar
• Butler, M.; Spearman, M. The Choice of Mammalian Cell Host and Possibilities for Glycosylation Engineering. Curr. Opin. Biotechnol. 2014, 30, 107–112.
   PubMed Web of Science ®Google Scholar
• Lalonde, M.E.; Durocher, Y. Therapeutic Glycoprotein Production in Mammalian Cells. J. Biotechnol. 2017, 251, 128–140.
   PubMed Web of Science ®Google Scholar
• Dumont, J.; Euwart, D.; Mei, B.; Estes, S.; Kshirsagar, R. Human Cell Lines for Biopharmaceutical Manufacturing: History, Status, and Future Perspectives. Crit. Rev. Biotechnol. 2016, 36, 1110–1122.
   PubMed Web of Science ®Google Scholar
• Wells, E.; Robinson, A.S. Cellular Engineering for Therapeutic Protein Production: Product Quality, Host Modification, and Process Improvement. Biotechnol. J. 2017, 12.1600105.
   Web of Science ®Google Scholar
• Rajabibazl, M.; Rasaee, M.J.; Forouzandeh, M.; Rahimpour, A. Retroviral Transduction of Fluonanobody and the Variable Domain of Camelid Heavy-Chain Antibodies to Chicken Embryonic Cells. Iran J. Immunol. 2013, 10, 247–258.
   PubMed Web of Science ®Google Scholar
• Lai, T.; Yang, Y.; Ng, S.K. Advances in Mammalian Cell Line Development Technologies for Recombinant Protein Production. Pharmaceuticals (Basel). 2013, 6, 579–603.
   PubMedGoogle Scholar
• Zhang, L.; Inniss, M.C.; Han, S.; Moffat, M.; Jones, H.; Zhang, B.; Cox, W.L.; Rance, J.R.; Young, R.J. Recombinase-Mediated Cassette Exchange (RMCE) for Monoclonal Antibody Expression in the Commercially Relevant CHOK1SV Cell Line. Biotechnol. Prog. 2015, 31, 1645–1656.
   PubMed Web of Science ®Google Scholar
• Balasubramanian, S.; Rajendra, Y.; Baldi, L.; Hacker, D.L.; Wurm, F.M. Comparison of Three Transposons for the Generation of Highly Productive Recombinant CHO Cell Pools and Cell Lines. Biotechnol. Bioeng. 2016, 113, 1234–1243.
   PubMed Web of Science ®Google Scholar
• Kawabe, Y.; Inao, T.; Komatsu, S.; Huang, G.; Ito, A.; Omasa, T.; Kamihira, M. Improved Recombinant Antibody Production by CHO Cells Using a Production Enhancer DNA Element with Repeated Transgene Integration at a Predetermined Chromosomal site. J. Biosci. Bioeng. 2017, 123, 390–397.
   PubMed Web of Science ®Google Scholar
• Kawabe, Y.; Komatsu, S.; Komatsu, S.; Murakami, M.; Ito, A.; Sakuma, T.; Nakamura, T.; Yamamoto, T.; Kamihira, M. Targeted Knock-in of an scFv-Fc Antibody Gene into the Hprt Locus of Chinese Hamster Ovary Cells Using CRISPR/Cas9 and CRIS-PITCh Systems. J. Biosci. Bioeng. 2018, 125, 599–605.
   PubMed Web of Science ®Google Scholar
• Oberbek, A.; Matasci, M.; Hacker, D.L.; Wurm, F.M. Generation of Stable, High-Producing CHO Cell Lines by Lentiviral Vector-Mediated Gene Transfer in Serum-Free Suspension Culture. Biotechnol. Bioeng. 2011, 108, 600–610.
   PubMed Web of Science ®Google Scholar
• Spencer, H.T.; Denning, G.; Gautney, R.E.; Dropulic, B.; Roy, A.J.; Baranyi, L.; Gangadharan, B.; Parker, E.T.; Lollar, P.; Doering, C.B. Lentiviral Vector Platform for Production of Bioengineered Recombinant Coagulation Factor VIII. Mol. Ther. 2011, 19, 302–309.
   PubMed Web of Science ®Google Scholar
• Benskey, M.J.; Manfredsson, F.P. Lentivirus Production and Purification. Methods Mol. Biol. 2016, 1382, 107–114.
   PubMedGoogle Scholar
• Etemadzadeh, M.H.; Arashkia, A.; Roohvand, F.; Ahani, R.; Mohajel, N.; Baniasadi, V.; Norouzian, D.; Azadmanesh, K. Expression of a Biotin Acceptor Peptide-Containing Protein with Potential Incorporation on the Lentiviral Envelope as a Viral Surface Engineering Platform. Res. Pharm. Sci. 2015, 10, 268–274.
   PubMed Web of Science ®Google Scholar
• Girod, P.-A.; Nguyen, D.-Q.; Calabrese, D.; Puttini, S.; Grandjean, M.; Martinet, D.; Regamey, A.; Saugy, D.; Beckmann, J.S.; Bucher, P.; Mermod, N. Genome-Wide Prediction of Matrix Attachment Regions That Increase Gene Expression in Mammalian Cells. Nat. Methods 2007, 4, 747–753.
   PubMed Web of Science ®Google Scholar
• Chen, S.J.; Wang, W.; Zhang, F.Y.; Jia, Y.L.; Wang, X..; Guo, X.; Chen, S.N.; Gao, J.H.; Wang, T.Y. A Chimeric HS4 Insulator-Scaffold Attachment Region Enhances Transgene Expression in Transfected Chinese Hamster Ovary Cells. FEBS Open Bio. 2017, 7, 2021–2030.
   PubMed Web of Science ®Google Scholar
• Kostyrko, K.; Neuenschwander, S.; Junier, T.; Regamey, A.; Iseli, C.; Schmid-Siegert, E.; Bosshard, S.; Majocchi, S.; Le Fourn, V.; Girod, P.-A.; et al. MAR-Mediated Transgene Integration into Permissive Chromatin and Increased Expression by Recombination Pathway Engineering. Biotechnol. Bioeng. 2017, 114, 384–396.
   PubMed Web of Science ®Google Scholar
• Barde, I.; Salmon, P.; Trono, D. Production and Titration of Lentiviral Vectors. Curr. Protoc. Neurosci. 2010,
   PubMedGoogle Scholar
• Chu, L.; Blumentals, I.; Maheshwari, G. Production of Recombinant Therapeutic Proteins by Mammalian Cells in Suspension Culture. Methods Mol. Biol. 2005, 308, 107–121.
   PubMedGoogle Scholar
• Lee, C.; Kim, J.; Shin, S.G.; Hwang, S. Absolute and Relative QPCR Quantification of Plasmid Copy Number in Escherichia coli. J. Biotechnol. 2006, 123, 273–280.
   PubMed Web of Science ®Google Scholar
• Mason, M.; Sweeney, B.; Cain, K.; Stephens, P.; Sharfstein, S.T. Identifying Bottlenecks in Transient and Stable Production of Recombinant Monoclonal-Antibody Sequence Variants in Chinese Hamster Ovary Cells. Biotechnol. Prog. 2012, 28, 846–855.
   PubMed Web of Science ®Google Scholar
• Dang, Q.; Auten, J.; Plavec, I. Human Beta Interferon Scaffold Attachment Region Inhibits de Novo Methylation and Confers Long-Term, Copy Number-Dependent Expression to a Retroviral Vector. J. Virol. 2000, 74, 2671–2678.
   PubMed Web of Science ®Google Scholar
• Ramezani, A.; Hawley, T.S.; Hawley, R.G. Performance- and Safety-Enhanced Lentiviral Vectors Containing the Human Interferon-Beta Scaffold Attachment Region and the Chicken Beta-Globin Insulator. Blood 2003, 101, 4717–4724.
   PubMed Web of Science ®Google Scholar
• Ayyar, B.V.; Arora, S.; Ravi, S.S. Optimizing Antibody Expression: The Nuts and Bolts. Methods. 2017, 116, 51–62.
   PubMed Web of Science ®Google Scholar
• Fischer, S.; Handrick, R.; Otte, K. The Art of CHO Cell Engineering: A Comprehensive Retrospect and Future Perspectives. Biotechnol. Adv. 2015, 33, 1878–1896.
   PubMed Web of Science ®Google Scholar
• Kumar, P.; Woon-Khiong, C. Optimization of Lentiviral Vectors Generation for Biomedical and Clinical Research Purposes: contemporary Trends in Technology Development and Applications. Curr. Gene. Ther. 2011, 11, 144–153.
   PubMed Web of Science ®Google Scholar
• Ho, S.C.; Bardor, M.; Feng, H.; Tong, Y.W.; Song, Z.; Yap, M.G.; Yang, Y. IRES-Mediated Tricistronic Vectors for Enhancing Generation of High Monoclonal Antibody Expressing CHO Cell Lines. J. Biotechnol. 2012, 157, 130–139.
   PubMed Web of Science ®Google Scholar
• Borman, A.M.; Le Mercier, P.; Girard, M.; Kean, K.M. Comparison of Picornaviral IRES-Driven Internal Initiation of Translation in Cultured Cells of Different Origins. Nucleic Acids Res. 1997, 25, 925–932.
   PubMed Web of Science ®Google Scholar
• Wang, T.Y.; Han, Z.M.; Chai, Y.R.; Zhang, J.H. A Mini Review of MAR-Binding Proteins. Mol. Biol. Rep. 2010, 37, 3553–3560.
   PubMed Web of Science ®Google Scholar
• Liebich, I.; Bode, J.; Reuter, I.; Wingender, E. Evaluation of Sequence Motifs Found in Scaffold/Matrix-Attached Regions (S/MARs). Nucleic Acids Res. 2002, 30, 3433–3442.
   PubMed Web of Science ®Google Scholar
• Harraghy, N.; Buceta, M.; Regamey, A.; Girod, P. A.; Mermod, N. Using Matrix Attachment Regions to Improve Recombinant Protein Production. Methods Mol. Biol. 2012, 801, 93–110.
   PubMedGoogle Scholar
• Murray, L.; Travis, M.; Luens-Abitorabi, K.; Olsson, K.; Plavec, I.; Forestell, S.; Hanania, E.G.; Hill, B. Addition of the Human Interferon Beta Scaffold Attachment Region to Retroviral Vector Backbones Increases the Level of in Vivo Transgene Expression among Progeny of Engrafted Human Hematopoietic Stem Cells. Hum. Gene Ther. 2000, 11, 2039–2050.
   PubMed Web of Science ®Google Scholar
• Baranyi, L.; Doering, C.B.; Denning, G.; Gautney, R.E.; Harris, K.T.; Spencer, H.T.; Roy, A.; Zayed, H.; Dropulic, B. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Hum. Gene Ther. Methods 2013, 24, 214–227.
   PubMed Web of Science ®Google Scholar
• Mufarrege, E.F.; Antuna, S.; Etcheverrigaray, M.; Kratje, R.; Prieto, C. Development of Lentiviral Vectors for Transient and Stable Protein Overexpression in Mammalian Cells. A New Strategy for Recombinant Human FVIII (rhFVIII) Production. Protein Expr. Purif. 2014, 95, 50–56.
   PubMed Web of Science ®Google Scholar
• Zhang, J.H.; Wang, X.Y.; Wang, T.Y.; Wang, F.; Dong, W.H.; Wang, L.; Zhao, C.P.; Chai, S.J.; Yang, R.; Li, Q. Distance Effect of Matrix Attachment Regions on Transgene Expression in Stably Transfected Chinese Hamster Ovary Cells. Biotechnol. Lett. 2014, 36, 1937–1943.
   PubMed Web of Science ®Google Scholar
• Jia, Y.L.; Guo, X.; Wang, X.C.; Wang, T.Y. Human Genome-Derived TOP1 Matrix Attachment Region Enhances Transgene Expression in the Transfected CHO Cells. Biotechnol. Lett. 2019.
   PubMed Web of Science ®Google Scholar
• Bayat, H.; Hossienzadeh, S.; Pourmaleki, E.; Ahani, R.; Rahimpour, A. Evaluation of Different Vector Design Strategies for the Expression of Recombinant Monoclonal Antibody in CHO Cells. Prep. Biochem. Biotechnol. 2018, 48, 160–164.
   PubMed Web of Science ®Google Scholar