Synthesis and Efficient Isolation Procedure for γ-Linked Fluorescein Methotrexate.

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• Abbreviations: MTX, Methotrexate (4‐amino‐4‐deoxy‐N 10_ methylpteroylglutamic acid); F‐MTX, MTX linked via 1,5‐diaminopentane to fluorescein isothiocyanate; DHFR, dihydrofolate reductase; HPLC, high pressure liquid chromatography; EDC, l‐ethyl‐3‐(3′‐dimethylaminopropyl) carbodiimide hydrochloride; DMSO, dimethylsulfoxide; F‐DAP, 1,5‐diaminopentane linked to fluorescein isothiocyanate; DEAE‐cellulose, diethylaminoethyl‐cellulose
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