Dietary supplementation of garlic, propolis, and wakame improves recuperation in cadmium exposed Japanese medaka fish (Oryzias latipes)

References

• Ali, H.; Khan, E.; Ilahi, I. Environmental and Ecotoxicology of Hazardous Heavy Metals: environmental Persistence, Toxicity, and Bioaccumulation. J. Chem. 2019, 2019, 1–14. DOI: 10.1155/2019/6730305.
   Web of Science ®Google Scholar
• Moulis, J. M. Cellular Mechanisms of Cadmium Toxicity Related to the Homeostasis of Essential Metals. Biometals. 2010, 23, 877–896. DOI: 10.1007/s10534-010-9336-y.
   PubMed Web of Science ®Google Scholar
• Ammendola, S.; Cerasi, M.; Battistoni, A. Deregulation of Transition Metals Homeostasis is a Key Feature of Cadmium Toxicity in Salmonella. Biometals. 2014, 27, 703–714. DOI: 10.1007/s10534-014-9763-2.
   PubMed Web of Science ®Google Scholar
• Ahad, R. I. A.; Syiem, M. B. Influence of Calcium on Cadmium Uptake and Toxicity to the Cyanobacterium Nostoc Muscorum Meg 1. Biotechnol. Res. Innov. 2019, 3, 231–241. DOI: 10.1016/j.biori.2019.06.002.
   Google Scholar
• Choong, G.; Liu, Y.; Templeton, D. M. Interplay of Calcium and Cadmium in Mediating Cadmium Toxicity. Chem. Biol. Interact. 2014, 211, 54–65. DOI: 10.1016/j.cbi.2014.01.007.
   PubMed Web of Science ®Google Scholar
• Vesey, D. A. Transport Pathways for Cadmium in the Intestine and Kidney Proximal Tubule: Focus on the Interaction with Essential Metals. Toxicol. Lett. 2010, 198, 13–19. DOI: 10.1016/j.toxlet.2010.05.004.
   PubMed Web of Science ®Google Scholar
• Zhang, H.; Pang, Z.; Han, C. Undaria Pinnatifida (Wakame): a Seaweed with Pharmacological Properties. Sci. Int. 2014, 2, 32–36.
   Google Scholar
• Scandalios, J. G. Oxidative Stress: Molecular Perception and Transduction of Signals Triggering Antioxidant Gene Defenses. Braz. J. Med. Biol. Res. 2005, 38, 995–1014. DOI: 10.1590/s0100-879x2005000700003.
   PubMed Web of Science ®Google Scholar
• Kumar, P.; Singh, A. Cadmium Toxicity in Fish: An Overview. GERF Bull. Biosci. 2010, 1, 41–47.
   Google Scholar
• Mosbaha, A.; Guerbe, H.; Boussettaa, H.; Banni, M. Effects of Dietary Garlic against Cadmium Induced Immunotoxicity in Sea Bass Head, Kidney and Liver Tissues: A Transcriptomic Approach. J. Environ. Chem. Toxicol 2017, 1, 9–14.
   Google Scholar
• Tandon, S. K.; Singh, S.; Prasad, S. Influence of Garlic on the Disposition and Toxicity of Lead and Cadmium in Rat. Pharmaceut. Biol 2001, 39, 450–454. DOI: 10.1076/phbi.39.6.450.5887.
   Web of Science ®Google Scholar
• Kamiya, T.; Izumi, M.; Hara, H.; Adachi, T. Propolis Suppresses CdCl2-Induced Cytotoxicity of COS7 Cells through the Prevention of Reactive Oxygen Species Accumulation. Biol. Pharm. Bull 2012, 35, 1126–1131.
   PubMed Web of Science ®Google Scholar
• El-Masry, T. A.; Emara, A. M.; El-Shitany, N. A. Possible Protective Effects of Propolis against Lead-Induced Neurotoxicity in Animal Model. J. Evolut. Biol. Res. 2011, 3, 4–11.
   Web of Science ®Google Scholar
• Talas, Z. S.; Gulhan, M. F.; Erdogan, K.; Orun, I. Antioxidant Effects of Propolis on Carp Cyprinus Carpio Exposed to Arsenic: Biochemical and Histopathologic Findings. Dis. Aquat. Organ. 2014, 108, 241–249. DOI: 10.3354/dao02714.
   PubMed Web of Science ®Google Scholar
• Yang, H.; Xing, R.; Liu, S.; Li, P. Effect of Fucoxanthin Administration on Thyroid Gland Injury Induced by Cadmium in Mice. Biol. Trace Elem. Res. 2020, in press. doi:10.1007/s12011-020-02291-9.
   Web of Science ®Google Scholar
• Nishibori, N.; Sagara, T.; Hiroi, T.; Sawaguchi, M.; Itoh, M.; Her, S.; Morita, K. Protective Effect of Undaria Pinnatifida Sporophyll Extract on Iron Induced Cytotoxicity and Oxidative Stress in PC12 Neuronal Cells. Phytopharmacology. 2012, 2, 271–284.
   Google Scholar
• Padilla, S.; Cowden, J.; Hinton, D. E.; Yuen, B.; Law, S.; Kullman, S. W.; Johnson, R.; Hardman, R. C.; Flynn, K.; Au, D. W. Use of Medaka in Toxicity Testing. Curr. Protoc. Toxicol. 2009, Chapter 1, Unit1.10
   PubMedGoogle Scholar
• Sasado, T.; Tanaka, M.; Kobayashi, K.; Sato, T.; Sakaizumi, M.; Naruse, K. The National BioResource Project Medaka (NBRP Medaka): An Integrated Bioresource for Biological and Biomedical Sciences. Exp. Anim. 2010, 59, 13–23. DOI: 10.1538/expanim.59.13.
   PubMed Web of Science ®Google Scholar
• Ishikawa, Y. Medakafish as a Model System for Vertebrate Developmental Genetics. Bioessays. 2000, 22, 487–495. DOI: 10.1002/(SICI)1521-1878(200005)22:5<487::AID-BIES11>3.0.CO;2-8.
   PubMed Web of Science ®Google Scholar
• Wittbrodt, J.; Shima, A.; Schartl, M. Medaka – A Model Organism from the Far East. Nat. Rev. Genet. 2002, 3, 53–64. [Database] DOI: 10.1038/nrg704.
   PubMed Web of Science ®Google Scholar
• Furusawa, R.; Okinaka, Y.; Nakai, T. Betanodavirus Infection in the Freshwater Model Fish Medaka (Oryzias Latipes). J. General Virol. 2006, 87, 2333–2339. DOI: 10.1099/vir.0.81761-0.
   PubMed Web of Science ®Google Scholar
• Abdallah, M. A. M. Trace Metal Behaviour in Mediterranean-Climate Coastal Bay: El-Mex Bay, Egypt and Its Coastal Environment. Global J. Environ. Res. 2008, 2, 23–29.
   Google Scholar
• Ohimain, E.; Jonathan, G.; Abah, S. O. Variations in Heavy Metal Concentrations following the Dredging of an Oil Well Access Canal in the Niger Delta. Adv. Biol. Res. 2008, 2, 97–103.
   Google Scholar
• Fakayode, S. O.; Onianwa, P. C. Heavy Metal Contamination of Soil, and Bioaccumulation in guinea Grass (Panicum Maximum) around Ikeja Industrial Estate, Lagos, Nigeria. Environ. Geol. 2002, 43, 145–150.
   Web of Science ®Google Scholar
• Bradford, M. M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of protein-dye binding. Anal. Biochem. 1976, 72, 248–254. DOI: 10.1006/abio.1976.9999.
   PubMed Web of Science ®Google Scholar
• Ellman, G. L. Tissue Sulfhydryl Groups. Arch. Biochem. Biophys. 1959, 82, 70–77. DOI: 10.1016/0003-9861(59)90090-6.
   PubMed Web of Science ®Google Scholar
• Ighodaro, O. M.; Akinloye, O. A. First Line Defence Antioxidants-Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPX): Their Fundamental Role in the Entire Antioxidant Defence Grid. Alex. J. Med 2018, 54, 287–293. DOI: 10.1016/j.ajme.2017.09.001.
   Web of Science ®Google Scholar
• Pavarino, E. C.; Russo, A.; Galbiatti, A. L.; Almeida, W. P.; Bertollo, E. M. Bosynthesis and Mechanism of Action. In Biochemistry and Mechanism of Action; Labrou, N.; Flemetakis, E, Eds.; Nova Science Publishers, Inc: New York, 2013; pp. 1–31.
   Google Scholar
• Pizzorno, J. E.; Katzinger, J. J. Glutathione: Physiological and Clinical Relevance. j. Restorat. Med. 2012, 1, 24–37. DOI: 10.14200/jrm.2012.1.1002.
   Google Scholar
• Mozsár, A.; Boros, G.; Saly, P.; Antal, L.; Nagy, S. A. Relationship between Fulton’s Condition Factor and Proximate Body Composition in Three Freshwater Fish Species. J. Appl. Ichthyol. 2015, 31, 315–320. DOI: 10.1111/jai.12658.
   Web of Science ®Google Scholar
• Hopkins, K. D. Reporting Fish Growth: A Review of the Basics. J. World Aquaculture Soc. 1992, 23, 173–179. DOI: 10.1111/j.1749-7345.1992.tb00766.x.
   Google Scholar
• Li, Z. H.; Zlabek, V.; Grabic, R.; Li, P.; Machova, J.; Velisek, J.; Randak, T. Effects of Exposure to Sublethal Propiconazole on the Antioxidant Defense System and Na+-K+-ATPase Activity in Brain of Rainbow Trout, Oncorhynchus mykiss. Aquat. Toxicol. 2010, 98, 297–303. DOI: 10.1016/j.aquatox.2010.02.017.
   PubMed Web of Science ®Google Scholar
• Le Cren, E. D. The Length-Weight Relationships and Seasonal Cycle in Gonad Weight and Condition in the Perch (Perca Fluviatilis). J. Anim. Ecol. 1951, 20, 201–219. DOI: 10.2307/1540.
   Web of Science ®Google Scholar
• Sutton, S. G.; Bult, T. P.; Haedrich, R. L. Relationships among Fat Weight, Body Weight, Water Weight and Condition Factors in Wild Atlantic Salmon Parr. Transactions Am. Fish Soc. 2000, 129, 527–538. DOI: 10.1577/1548-8659(2000)129<0527:RAFWBW>2.0.CO;2.
   Web of Science ®Google Scholar
• National Research Council (US) Institute for Laboratory Animal Research. Guidance for the Description of Animal Research in Scientific Publications. National Academies Press (US): Washington (DC), 2011. Available from: https://www.ncbi.nlm.nih.gov/books/NBK84210/.
   Google Scholar
• Li, H.; Mai, K.; Ai, Q.; Zhang, C.; Zhang, L. Effects of Dietary Squid Viscera Meal on Growth and Cadmium Accumulation in Tissues of Large Yellow Croaker. Front. Agric. China. 2009, 3, 78–83. DOI: 10.1007/s11703-009-0012-3.
   Google Scholar
• Miranda, T.; Vieira, L. R.; Guilhermino, L. Neurotoxicity, Behavior, and Lethal Effects of Cadmium, Microplastics, and Their Mixtures on Pomatoschistus Microps Juveniles from Two Wild Populations Exposed under Laboratory Conditions―Implications to Environmental and Human Risk Assessment. Ijerph. 2019, 16, 2857. DOI: 10.3390/ijerph16162857.
   Google Scholar
• Abdeltawwab, M.; Wafeek, M.; Elghobashy, H.; Fitzsimmons, K.; Diab, A. S. Response of Nile Tilapia, Oreochromis Niloticus (L.) to Environmental Cadmium Toxicity during Organic Selenium Supplementation. J. World Aquac. Soc. 2010, 41, 106–114. DOI: 10.1111/j.1749-7345.2009.00317.x.
   Web of Science ®Google Scholar
• Giari, L.; Manera, M.; Simoni, E.; Dezfuli, S. Cellular Alterations in Different Organs of European Sea Bass Dicentrarchus Labrax (L.) Exposed to Cadmium. Chemosphere. 2007, 67, 1171–1181. DOI: 10.1016/j.chemosphere.2006.10.061.
   PubMed Web of Science ®Google Scholar
• Xiong, X.; Li, H.; Qiu, N.; Su, L.; Huang, Z.; Song, L.; Wang, J. Bioconcentration and Depuration of Cadmium in the Selected Tissues of Rare Minnow (Gobiocypris Rarus) and the Effect of Dietary Mulberry Leaf Supplementation on Depuration. Environ. Toxicol. Pharmacol. 2020, 73, 103278. DOI: 10.1016/j.etap.2019.103278.
   PubMed Web of Science ®Google Scholar
• - Fernandes, A.; Rontainhas-Fernandes, A.; Rocha, E.; Reis-Henriques, M. A. The Effect of Paraquat on Hepatic EROD Activity, Liver and Gonadal Histology in Males and Females of Nile Tilapia, Oreochromis Niloticus, Exposed at Different Temperatures. Arch. Environ. Contam. Toxicol. 2006, 51, 626–632.
   PubMed Web of Science ®Google Scholar
• Dang, Z. C.; Berntssen, M. H. G.; Lundebye, A. K.; Flik, G.; Bonga, S. E. W.; Lock, R. A. C. Metallothionein and Cortisol Receptor Expression in Gills of Atlantic Salmon, Salmo Salar, Exposed to Dietary Cadmium. Aquat. Toxicol. 2001, 53, 91–101. DOI: 10.1016/s0166-445x(00)00168-5.
   PubMed Web of Science ®Google Scholar
• Playle, R. C. Modelling Metal Interactions at Fish Gills. Sci. Total Environ. 1998, 219, 147–163. DOI: 10.1016/S0048-9697(98)00232-0.
   Web of Science ®Google Scholar
• Kim, S. G.; Jee, J. H.; Kang, J. C. Cadmium Accumulation and Elimination in Tissues of Juvenile Olive Flounder, Paralichthys Olivaceus after Sub-Chronic Cadmium Exposure. Environ. Pollut. 2004, 127, 117–123. DOI: 10.1016/s0269-7491(03)00254-9.
   PubMed Web of Science ®Google Scholar
• Boonpeng, S.; Siripongvutikorn, S.; Sae-Wong, C.; Sutthirak, P. The Antioxidant and anti-Cadmium Toxicity Properties of Garlic Extracts. Food Sci Nutr. 2014, 2, 792–801. DOI: 10.1002/fsn3.164.
   PubMedGoogle Scholar
• Kay, Y. H.; Yang, W. J.; Kim, H. T.; Lee, Y. D.; Kang, B.; Ryu, J. H.; Jeon, R.; Kim, S. G. Ajoene, a Stable Garlic by-Product, Has an Antioxidant Effect through Nrf2-Mediated Glutamate-Cysteine Ligase Induction in HepG2 Cells and Primary Hepatocytes. J. Nutr. 2010, 140, 1211–1219. DOI: 10.3945/jn.110.121277.
   PubMed Web of Science ®Google Scholar
• Amagase, H. Clarifying the Real Bioactive Constituents of Garlic. J. Nutr. 2006, 136, 716S–725S. DOI: 10.1093/jn/136.3.716S.
   PubMed Web of Science ®Google Scholar
• Robert, V.; Mouillé, B.; Mayeur, C.; Michaud, M.; Blachier, F. Effects of the Garlic Compound Diallyl Disulfide on the Metabolism, Adherence and Cell Cycle of HT-29 Colon Carcinoma Cells: evidence of Sensitive and Resistant Sub-Populations. Carcinogenesis. 2001, 22, 1155–1161. DOI: 10.1093/carcin/22.8.1155.
   PubMed Web of Science ®Google Scholar
• José, P.; Ana, E. G.; Perla, D. M.; Diana, B.; Omar, N. M.; Rogelio, H. P. Diallyl Disulfide Ameliorates Gentamicin-Induced Oxidative Stress and Nephropathy in Rat. Eur. J. Pharmacol. 2003, 473, 71–78.
   PubMed Web of Science ®Google Scholar
• Lawal, O. A.; Elizabeth, M. E. The Chemopreventive Effects of Aged Garlic Extract against Cadmium-Induced Toxicity. Environ. Toxicol. Pharmacol. 2011, 32, 266–274. DOI: 10.1016/j.etap.2011.05.012.
   PubMed Web of Science ®Google Scholar
• Bankova, V.; Christov, R.; Kujumgiev, A.; Marcucci, M. C.; Popov, S. Chemical Composition and Antibacterial Activity of Brazilian Propolis. Z. Naturforsch. C. J. Biosci. 1995, 50, 167–172. DOI: 10.1515/znc-1995-3-402.
   PubMed Web of Science ®Google Scholar
• Ahn, M. R.; Kunimasa, K.; Kumazawa, S.; Nakayama, T.; Kaji, K.; Uto, Y.; Hori, H.; Nagasawa, H.; Ohta, T. Correlation between Antiangiogenic Activity and Antioxidant Activity of Various Components from Propolis. Mol. Nutr. Food Res. 2009, 53, 643–651. DOI: 10.1002/mnfr.200800021.
   PubMed Web of Science ®Google Scholar
• Jung, W. K.; Choi, I.; Lee, D. Y.; Yea, S. S.; Choi, Y. H.; Kim, M. M.; Park, S. G.; Seo, S. K.; Lee, S. W.; Lee, C. M.; et al. Caffeic Acid Phenethyl Ester Protects Mice from Lethal Endotoxin Shock and Inhibits lipopolysaccharide-induced cyclooxygenase-2 and inducible nitric oxide synthase expression in RAW 264.7 macrophages via the p38/ERK and NF-kappaB pathways. Int. J. Biochem. Cell Biol.. 2008, 40, 2572–2582. DOI: 10.1016/j.biocel.2008.05.005.
   PubMed Web of Science ®Google Scholar
• Kujumgiev, A.; Tsvetkova, I.; Serkedjieva, Y.; Bankova, V.; Christov, R.; Popov, S. Antibacterial, Antifungal and Antiviral Activity of Propolis of Different Geographic Origin. J. Ethnopharmacol. 1999, 64, 235–240. DOI: 10.1016/s0378-8741(98)00131-7.
   PubMed Web of Science ®Google Scholar
• Watabe, M.; Hishikawa, K.; Takayanagi, A.; Shimizu, N.; Nakaki, T. Caffeic Acid Phenethyl Ester Induces Apoptosis by Inhibition of NFkappaB and Activation of Fas in Human Breast Cancer MCF-7 Cells. J. Biol. Chem. 2004, 279, 6017–6026. DOI: 10.1074/jbc.M306040200.
   PubMed Web of Science ®Google Scholar
• Sato, M.; Hosokawa, T.; Yamaguchi, T.; Nakano, T.; Muramoto, K.; Kahara, T.; Funayama, K.; Kobayashi, A.; Nakano, T. Angiotensin I-Converting Enzyme Inhibitory Peptides Derived from Wakame (Undaria Pinnatifida) and Their Antihypertensive Effect in Spontaneously Hypertensive Rats. J. Agric. Food Chem.. 2002, 50, 6245–6252. DOI: 10.1021/jf020482t.
   PubMed Web of Science ®Google Scholar
• Hu, T.; Liu, D.; Chen, Y.; Wu, J.; Wang, S. Antioxidant Activity of Sulfated Polysaccharide Fractions Extracted from Undaria Pinnitafida in Vitro. Int. J. Biol. Macromol. 2010, 46, 193–198. DOI: 10.1016/j.ijbiomac.2009.12.004.
   PubMed Web of Science ®Google Scholar
• Cakmak, I.; Strbac, D.; Marschner, H. Activities of Hydrogen Peroxide Scavenging Enzymes in Germinated Wheat Seeds. J. Exp. Bot. 1993, 44, 127–132. DOI: 10.1093/jxb/44.1.127.
   Web of Science ®Google Scholar
• EL-Gazzar, A. M.; Astry, K. E.; El-Sayed, Y. S. Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis Niloticus L., Exposed to Sublethal Doses of Cadmium. Alex. J. Vet. Sci. 2014, 40, 29–43.
   Google Scholar
• Mehrpak, M.; Banaee, M.; Haghi, B. N.; Noori, A. Protective Effects of Vitamin C and Chitosan against Cadmium-Induced Oxidative Stress in the Liver of Common Carp (Cyprinus Carpio). Iranian J. Toxicol. 2015, 9, 1360–1367.
   Google Scholar
• Banaee, M.; Mehrpak, M.; Haghi, B. N.; Noori, A. Amelioration of Cadmium-Induced Changes in Biochemical Parameters of the Muscle of Common Carp (Cyprinus Carpio) by Vitamin C and Chitosan. Int. J. Aquat. Biol. 2015, 2, 362–371.
   Google Scholar
• Silvagno, F.; Vernone, A.; Pescarmona, P. The Role of Glutathione in Protecting against the Severe Inflammatory Response Triggered by COVID-19. Antioxidants. 2020, 9, 624. DOI: 10.3390/antiox9070624.
   Web of Science ®Google Scholar
• Forman, H. J.; Zhang, H.; Rinna, A. Glutathione: Overview of Its Protective Roles, Measurement, and Biosynthesis. Mol. Aspects Med. 2009, 30, 1–12.
   PubMed Web of Science ®Google Scholar
• Feoli, A. M.; Siqueira, I.; Almeida, L. M.; Tramontina, A. C.; Battu, C.; Wofchuk, S. T.; Gottfried, C.; Perry, M. L.; Gonçalves, C. A. Brain Glutathione Content and Glutamate Uptake Are Reduced in Rats Exposed to Pre- and Postnatal Protein Malnutrition. J. Nutr. 2006, 136, 2357–2361. DOI: 10.1093/jn/136.9.2357.
   PubMed Web of Science ®Google Scholar
• López-López, A. L.; Jaime, H. B.; Escobar-Villanueva, M. D. C.; Padilla, M. B.; Palacios, G. V.; Aguilar, F. J. A. Chronic Unpredictable Mild Stress Generates Oxidative Stress and Systemic Inflammation in Rats. Physiol. Behav. 2016, 161, 15–23. DOI: 10.1016/j.physbeh.2016.03.017.
   PubMed Web of Science ®Google Scholar
• Babula, P.; Masarik, M.; Adam, V.; Eckschlager, T.; Stiborova, M.; Trnkova, L.; Skutkova, H.; Provaznik, I.; Hubalek, J.; Kizek, R. Mammalian Metallothioneins: properties and Functions. Metallomics. 2012, 4, 739–750. DOI: 10.1039/c2mt20081c.
   PubMed Web of Science ®Google Scholar
• Carginale, V.; Scudiero, R.; Capasso, C.; Capasso, A.; Kille, P.; di Prisco, G.; Parisi, E. Cadmium-Induced Differential Accumulation of Metallothionein Isoforms in the Antarctic Icefish, Which Exhibits No Basal Metallothionein Protein but High Endogenous mRNA Levels. Biochem. J. 1998, 332, 475–481. DOI: 10.1042/bj3320475.
   PubMed Web of Science ®Google Scholar
• Gaschler, M. M.; Stockwell, B. R. Lipid Peroxidation in Cell Death. Biochem. Biophys. Res. Commun. 2017, 482, 419–425. DOI: 10.1016/j.bbrc.2016.10.086.
   PubMed Web of Science ®Google Scholar
• Bhadauria, M.; Shukla, S.; Mathur, R.; Agrawal, O. P.; Shrivastava, S.; Johri, S.; Joshi, D.; Singh, V.; Mittal, D.; Nirala, K. S. Hepatic Endogenous Defense Potential of Propolis after Mercury Intoxication. Integr. Zool. 2008, 3, 311–321. DOI: 10.1111/j.1749-4877.2008.00103.x.
   PubMed Web of Science ®Google Scholar
• Bechard, K. M.; Gillis, P. L.; Wood, C. M. Trophic Transfer of Cd from Larval Chironomids (Chironomus Riparius) Exposed via sediment or waterborne routes, to zebrafish (Danio rerio): tissue-specific and subcellular comparisons. Aquat. Toxicol. 2008, 90, 310–321. DOI: 10.1016/j.aquatox.2008.07.014.
   PubMed Web of Science ®Google Scholar
• Yagi, K. Lipid Peroxides, Free Radicals, and Diseases. In Active Oxygen, Lipid Peroxides, and Antioxidants; Yagi, K, Ed.; Japan Scientific Press: Tokyo, 1993; pp. 39–67.
   Google Scholar
• Halliwell, B.; Gutterridge, J. M. C. Free Radicals in Biology and Medicine; Clarendon (Oxford) Press: Oxford, 1989; pp.188–276.
   Google Scholar
• Cross, C. E.; Halliwell, B.; Borish, E. T.; Pryor, W. A.; Ames, B. N.; Saul, R. L.; McCord, J. M.; Harman, D. Oxygen Radicals and Human Diseases. Ann. Intern. Med. 1987, 107, 526–545. DOI: 10.7326/0003-4819-107-4-526.
   PubMed Web of Science ®Google Scholar
• Pérez-Torres, I.; Torres-Narváez, J.; Pedraza-Chaverri, J.; Rubio-Ruiz, M.; Díaz-Díaz, E.; del Valle-Mondragón, L.; Martínez-Memije, R.; Varela López, E.; Guarner-Lans, V. Guarner-Lans, V. Effect of the Aged Garlic Extract on Cardiovascular Function in Metabolic Syndrome Rats. Molecules. 2016, 21, 1425. DOI: 10.3390/molecules21111425.
   PubMed Web of Science ®Google Scholar