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Original Articles

Stabilization of α-Amylase, the Key Enzyme in Carbohydrates Properties Alterations, at Low pH

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Pages 1182-1196 | Received 28 Oct 2009, Accepted 02 Jan 2010, Published online: 13 Oct 2011

REFERENCES

  • Dill , K.A. 1990 . Dominant forces in protein folding . Biochemistry , 29 ( 30 ) : 7133 – 7155 .
  • Goto , A. and Fink , A.L. 1989 . Conformational states of beta-lactamase: Molten globule state at acidic and alkaline pH with high salt . Biochemistry , 28 ( 3 ) : 945 – 952 .
  • Volkin , D.B. and Klibanov , A.M. 1989 . “ Minimizing protein interactions ” . In Protein Function: A practical approach , Edited by: Creighton , T.E. 1 – 24 . Oxford, , UK : Information Press .
  • Christina , R. , Smith , R.A.G. and Dobson , C.M. 1994 . Structural characterization of highly- ordered ‘molten globule’ at low pH . Nature Structural Biology , 1 : 23 – 29 .
  • Kamiyama , T. , Sadahide , Y. , Nogusa , Y. and Gekko , K. 1999 . Polyols-induced molten globule of cytochrome c: An evidence for stabilization by hydrophobic interaction . Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology , 1434 : 44 – 57 .
  • Kataoka , M. , Hagihara , Y. , Mihara , K. and Goto , Y. 1993 . Molten globule of cytochrome c studied by small angle X-ray scattering . Journal of Molecular Biology , 229 : 591 – 596 .
  • Nishii , I. , Kataoka , M. , Tokunaga , F. and Goto , Y. 1994 . Cold denaturation of molten globule states of apomyoglobin and a profile for protein folding . Biochemistry , 33 ( 16 ) : 4903 – 4909 .
  • Christensen , H. and Pain , R.H. 1991 . Molten globule intermediates and protein folding . European Biophysics Journal , 19 : 221 – 229 .
  • Potekhin , S. and Pfeil , W. 1989 . Microcalorimetric studies of conformational transitions of ferrycytochrome c in acidic solution . Biophysical Chemistry , 34 ( 1 ) : 55 – 62 .
  • McClements , D.J. 2002 . Modulation of globular protein functionality by weakly interacting cosolvents . Critical Reviewes in Food Sciences and Nutrition , 42 : 417 – 471 .
  • Gekko , K. and Timasheff , S.N. 1981 . Thermodynamic and kinetic examination of protein stabilization by glycerol . Biochemistry , 20 ( 16 ) : 4677 – 4686 .
  • Gekko , K. and Ito , H. 1990 . Competing solvent effects of polyols and guanidine hydrochloride on protein stability . The Journal of Biochemistry , 107 : 572 – 577 .
  • Gekko , K. 1981 . Mechanism of polyol-induced protein stabilization: Solubility of amino acids and diglycine in aqueous polyol solutions . The Journal of Biochemistry , 90 ( 6 ) : 1633 – 1641 .
  • Xie , G. and Timasheff , S.N. 1997 . Mechanism of stabilization of ribonuclease A by sorbitol: Preferential hydration is greater for the denatured than for the native protein . Protein Science , 6 ( 1 ) : 211 – 222 .
  • Goto , Y. , Calciano , L.J. and Fink , A.L. 1990 . Acid induced unfolding of proteins . Proceedings of National Academy of Sciences, USA , 87 : 573 – 577 .
  • Rajendran , S. , Radha , C. and Prakash , V. 1995 . Mechanism of solvents induced thermal stabilization of α-amylase from Bacillus amyloliquefaciens . International Journal of Peptide and Protein Research , 45 : 122 – 128 .
  • Fink , A.L. , Calciano , L.J. , Kurotsu , T. and Palleros , D.R. 1994 . Classification of acid denaturation of proteins: Intermediates and unfolded states . Biochemistry , 33 : 12,504 – 12,511 .
  • Marco , J.L. , Bataus , L.A. , Valencia , F.F. , Ulhoa , C.J. , Astolfi-Filho , S. and Felix , C.R. 1996 . Purification and characterization of a truncated Bacillus subtillis α-amylase produced by Escherichia coli . Applied Microbiology and Biotechnology , 44 : 746 – 752 .
  • Svensson , B. 1994 . Protein engineering in α-amylase family: Catalytic mechanism, substrate specificity, and stability . Plant Molecular Biology , 25 : 141 – 157 .
  • MacGregor , E.A. , Janecek , S. and Svensson , B. 2001 . Relation of sequence and structure to specificity in the alpha amylase family of enzymes . Biochimica et Biophysica Acta , 1546 : 1 – 20 .
  • Dauter , Z. , Dauter , M. , Brzozowski , A.M. , Christensen , S. , Borchert , T.V. , Beier , L. , Wilson , K.S. and Devies , G.J. 1999 . X-ray structure of Novamyl, the five domain “maltogenic” alpha-amylase from Bacillus stearothermophilus: Maltose and ascarbose complexes at 1.7 A° resolution . Biochemistry , 38 : 8385 – 8392 .
  • Lowry , O.H. , Rosenborough , N.J. , Farr , A.L. and Randall , R.J. 1951 . Protein measurement with the Folin phenol reagent . Journal of Biological Chemistry , 193 : 265 – 275 .
  • Bernfeld , P. 1955 . α- and β- Amylases . Methods in Enzymology , 1 : 149 – 158 .
  • Foo , A.Y. and Bais , R. 1998 . Amylase measurement with 2-chloro-4-nitrophenyl maltotrioside as substrate . Clinica Chimica Acta , 272 : 137 – 147 .
  • Dupuy , G. , Hilaire , G. and Aubry , C. 1987 . Rapid determination of α-amylase activity by use of a new chromogenic substrate . Clinical Chemistry , 33/4 : 524 – 528 .
  • Dragani , B. , Cocco , R. , Principe , D.R. , Cicconetti , M. and Aceto , A. 2000 . Structural characterization of acid induced intermediates of human glutathione transferase P1 . 1. The International Journal of Biochemistry and Cell Biology , 32 : 725 – 736 .
  • Eftink , M.R. and Ghiron , C.A. 1982 . Fluorescence quenching studies with proteins . Analytical Biochemistry , 114 : 199 – 227 .
  • Yang , J.T. , Wu , C.S.C. and Martinez , H.M. 1986 . Calculation of protein conformation from circular dichroism . Methods in Enzymology , 130 : 208 – 269 .
  • Edwin , F. , Sharma , Y.V. and Jagannadham , M.V. 2002 . Stabilization of molten globule state of papain by urea . Biochemical and Biophysical Research Communication , 290 : 1441 – 1446 .
  • Wang , W. 1999 . Instability, stabilization, and formulation of liquid protein pharmaceuticals . International Journal of Pharmacology , 185 : 129 – 188 .
  • Pawar , S.A. and Deshpande , V.V. 2000 . Characterization of acid induced unfolding intermediates of glucose/xylose isomerase . European Journal of Biochemistry , 267 : 6331 – 6338 .
  • Engelhard , M. and Evans , P.A. 1995 . Kinetics of interaction of partially folded proteins with a hydrophobic dye: Evidence that molten globule character is maximal in early folding intermediates . Protein Science , 4 : 1553 – 1562 .
  • Rashid , F. , Sharma , S. , Baig , M.A. and Bano , A. 2006 . Effect of polyols and salts on the acid- induced state of human placental cystatin . Biochemistry (Moscow) , 71 : 619 – 626 .
  • Sathish , H.A. , Kumar , P.R. and Prakash , V. 2002 . Effect of urea at lower concentration on the structure of papain: Formation of a stable molten globule and its characterization . Indian Journal of Biochemistry and Biophysics , 39 : 155 – 162 .
  • Muralidhara , B.K. and Prakash , V. 2002 . Molten globule intermediates of human serum albumin in low concentration of urea . Indian Journal of Biochemistry and Biophysics , 39 : 318 – 324 .
  • Asghari , S.M. , Khajeh , K. , Moradian , F. , Ranjbar , B. and Naderi-Manesh , H. 2004 . Acid-induced conformational changes in Bacillus amyloliquefaciens α-amylase: Appearance of molten globule likes state . Enzyme Microbial Technology , 35 : 51 – 57 .
  • Machius , M. , Wiegand , G. and Huber , R. 1995 . Crystal structure of calcium depleted Bacillus licheniformis alpha-amylase at 2 . 2A resolution. Journal of Molecular Biology , 246 : 545 – 559 .
  • Jiang , J.X. and Landon , E. 1990 . Involvement of denaturation-like changes in Pseudomonas exotoxin A, hydrophobicity, and membrane penetration determined by characterization of pH and thermal transitions: Roles of two distinct conformationally altered states . Journal of Biological Chemistry , 265 : 8636 – 8641 .
  • Yadav , J.K. and Prakash , V. 2009 . Thermal stability of amylase in aqueous cosolvent system . Journal of Biosciences , 43 ( 3 )
  • Kendrick , B.S. , Chang , B.S. , Arakawa , T. , Peterson , B. , Randolph , T.W. , Manning , M.C. and Carpenter , J.F. 1997 . Preferential exclusion of sucrose from recombinant interleukin-1 receptor antagonist: Role in restricted conformational mobility and compaction of native state . Proceedings of National Academy of Sciences, USA , 94 : 1917 – 1922 .
  • Liu , Y. and Bolen , D.W. 1995 . The peptide backbone plays a dominant role in protein stabilization by naturally occurring osmolytes . Biochemistry , 34 : 12884 – 12891 .
  • Devaraj , K.B. , Kumar , P.R. and Prakash , V. 2009 . Characterization of acid-induced molten globule like state of ficin . International Journal of Biological Macromolecules , 45 ( 3 ) : 248 – 254 .
  • Kulkarni , A. , Gaikwad , S. and Rao , M. 2008 . pH induced structural alterations in an aspartic protease from Vigna radiate indicating an alkali induced molten globule state . International Journal of Biological Macromolecules , 43 ( 4 ) : 375 – 376 .

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