6,422
Views
14
CrossRef citations to date
0
Altmetric
Original Articles

Taqman-Based Duplex Real-Time Polymerase Chain Reaction Approach for the Detection and Quantification of Donkey and Pork Adulterations in Raw and Heat-Processed Meats

, , , &
Pages 629-638 | Received 13 Nov 2011, Accepted 06 Dec 2011, Published online: 08 Nov 2013

REFERENCES

  • Asensio , L. , Gonzalez , I. , Garcia , T. and Martin , R. 2008 . Determination of food authenticity by enzyme-linked immunosorbent assay (ELISA) . Food Control , 19 : 1 – 8 .
  • Cota-Rivas , M. and Vallejo-Cordoba , B.V. 1997 . Capillary electrophoresis for meat species differentiation . Journal of Capillary Electrophoresis , 4 : 195 – 199 .
  • Andrews , C.D. , Berger , R.G. , Mageau , R.P. , Schwab , B. and Jhonston , R.W. 1992 . Detection of the beef, sheep, deer, and horse meat in cooked meat products by enzyme-linked immunosorbent assay . Journal of AOAC International , 75 : 572 – 576 .
  • Chen , F.C. and Hsieh , Y.H. 2000 . Detection of pork in heat-processed meat products by monoclonal antibody-based ELISA . Journal of AOAC International , 83 : 79 – 85 .
  • Skarpeid , H. J. , Kvaal , K. and Hildrum , K. I. 1998 . Identification of animal species in ground meat mixtures by multivariate analysis of isoelectric focusing protein profiles . Electrophoresis , 18 : 3103 – 3109 .
  • Chikuni , K. , Tabata , T. , Kosigiyama , M. and Monma , M. 1994 . Polymerase chain reaction assay for detection of sheep and goat meats . Meat Science , 37 : 337 – 345 .
  • Kesmen , Z. , Şahin , F. and Yetim , H. 2007 . PCR assay for the identification of animal species in cooked sausages . Meat Science , 77 : 649 – 653 .
  • Mafra , I. , Ferreira , I.M. and Oliveira , M.B. 2008 . Food authentication by PCR-based methods . European Food Research and Technology , 227 : 649 – 665 .
  • Hird , H. , Goodier , R. and Hill , M. 2003 . Rapid detection of chicken and turkey in heated meat products using the polymerase chain reaction followed by amplicon visualisation with vistra green . Meat Science , 65 : 1117 – 1123 .
  • Rodriguez , M. A. , Garcia , T. , Gonzalez , I. , Hernandez , P.E. and Martin . 2005 . R. TaqMan real-time PCR for detection and quantitation of pork in meat mixtures . Meat Science , 70 : 113 – 120 .
  • Guoli , Z. , Mingguang , Z. , Zhijiang , Z. , Hongsheng , O. and Qiang , L. 1999 . Establishment and application of a polymerase chain reaction for the identification of beef . Meat Science , 51 : 233 – 236 .
  • Kesmen , Z. , Gulluce , A. , Sahin , F. and Yetim , H. 2009 . Identification of meat species by TaqMan-based real-time PCR assay . Meat Science , 82 : 444 – 449 .
  • Fajardo , V. , Gonzales , I. , Martin , I. , Rojas , M. , Hernandez , P.E. , Garcia , T. and Real-time , Martín, R. 2008 . PCR for detection and quantification of red deer (Cervus elaphus), fallow deer (Dama dama), and roe deer (Capreolus capreolus) in meat mixtures . Meat Science , 79 : 289 – 298 .
  • Mafra , I. , Silva , S.A. , Moreira , E.J.M.O. , Ferreira da Silva , C.S. and Oliveira , M.B.P.P. 2008 . Comparative study of DNA extraction methods for soybean derived food products . Food Control , 19 : 1183 – 1190 .
  • Soares , S. , Amaral , J.S. , Mafra , I. and Oliveira , M.B.P.P. 2010 . Quantitative detection of poultry meat adulteration with pork by a duplex PCR assay . Meat Science , 85 : 531 – 536 .
  • Lopez-Andreo , M. , Garrido-Pertierra , A. and Puyet , A. 2006 . Evaluation of postpolymerase chain reaction melting temperature analysis for meat species identification in mixed DNA samples . Journal of Agricultural and Food Chemistry , 54 : 7973 – 7978 .
  • Walker , J.A. , Hughes , D.A. , Anders , B.A. , Sinha , J. , Shewale , S.K. and Batzer , M.A. 2003 . Quantitative intra-short interspersed element PCR for species-specific DNA identification . Analytical Biochemistry , 316 : 259 – 269 .
  • Brodmann , P.D. and Moor , D. 2003 . Sensitive and semi-quantitative TaqManTM realtime polymerase chain reaction systems for the detection of beef (Bos taurus) and the detection of the family Mammalia in food and feed . Meat Science , 65 : 599 – 607 .
  • Chisholm , J. , Conyers , C. , Booth , C. , Lawley , W. and Hird , H. 2005 . The detection of horse and donkey using real-time PCR . Meat Science , 70 : 727 – 732 .
  • Dooley , J.J. , Paine , K.E. , Garrett , S.D. and Brown , H.M. 2004 . Detection of meat species using TaqMan real-time PCR assays . Meat Science , 68 : 431 – 438 .
  • Hird , H. , Goodier , R. , Schneede , K. , Boltz , C. , Chisholm , J. , Lloyd , J. and Popping , B. 2004 . Truncation of oligonucleotide primers confers specificity on real time-PCR assays for food authentication . Food Additives and Contaminants , 21 : 1035 – 1040 .
  • Hird , H. , Chisholm , J. and Brown , J. 2005 . The detection of commercial duck species in food using a single probe-multiple species-specific primer real-time PCR assay . European Food Research and Technology , 221 : 559 – 563 .
  • Krcmar , P. and Rencova , E. 2005 . Quantitative detection of species-specific DNA in feedstuffs and fish meals . Journal of Food Protection , 68 : 1217 – 1221 .
  • Zhang , C.L. , Fowler , M.R. , Scott , N.W. , Lawson , G. and Slater , A. A . 2007 . TaqMan real-time PCR system for the identification and quantification of bovine DNA in meats, milks and cheeses . Food Control , 18 : 1149 – 1158 .
  • Holland , P.M. , Abramson , R.D. , Watson , R. and Gelfand , D.H. 1991 . Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Termus aquaticus DNA polymerase . Proceedings of the National Academy of Sciences , 88 : 7276 – 7280 .
  • Köppel , R. , Ruf , J. , Zimmerli , F. and Breitenmoser , A. 2008 . Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, chicken and turkey . European Food Research and Technology , 227 : 1199 – 1203 .
  • Heptinstall , J. and Rapley , R. 2002 . “ Spectrophotometric analysis of nucleic acids ” . In The Nucleic Acid Protocols Handbook , Edited by: Rapley , R. 57 – 60 . Totowa , NJ : Humana Press .
  • Di Pinto , A. , Forte , V.T. , Conversano , M.C. and Tantillo , G.M. 2005 . Duplex polymerase chain reaction for detection of pork meat in horse meat fresh sausages from Italian retail sources . Food Control , 16 : 391 – 394 .
  • Krcmar , P. and Rencova , E. 2003 . Identification of species-specific DNA in feedstuffs . Journal of Agricultural and Food Chemistry , 51 : 7655 – 7658 .
  • Matsunaga , T. , Chikuni , K. , Tanabe , R. , Muoya , S. , Shibata , K. , Yamada , J. and Shinmura , Y. 1999 . A quick and simple method for the identification of meat species and meat products by PCR assay . Meat Science , 51 : 143 – 148 .
  • Köppel , R. , Zimmerli , F. and Breitenmoser , A. 2009 . Heptaplex real-time PCR for the identification and quantification of DNA from beef, pork, chicken, turkey, horse meat, sheep (mutton) and goat . European Food Research and Technology , 230 : 125 – 133 .
  • Che Man , Y.B. , Mustafa , S. , Mokhtar , N.F.K. , Nordina , N. and Sazilia , A.Q. “ Porcine-specific polymerase chain reaction assay based on mitochondrial d-loop gene for identification of pork in raw meat ” . In International Journal of Food Properties doi: 10.1080/10942911003754692

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.