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Original Articles

Fast Separation of Capsaicinoids from Peppers by Reversed Phase Ultra-Performance Liquid Chromatography: Comparation with Traditional High-Performance Liquid Chromatography Methods

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Pages 984-992 | Received 07 Mar 2015, Accepted 10 May 2015, Published online: 18 Feb 2016

REFERENCES

  • Constant, H.L.; Cordell, G.A.; West, D.P. Nonivamide, a Constituent of Capsicum Oleoresin. Journal of Natural Products 1996, 59, 425–426.
  • Constant, H.L.; Cordell, G.A.; West, D.P.; Johnson, J.H. Separation and Quantification of Capsaicinoids Using Complexation Chromatography. Journal of Natural Products 1995, 58, 1925–1928.
  • Giuffrida, D.; Dugo, P.; Torre, G.; Bignardi, C.; Cavazza, A.; Corradini, C.; Dugo, G. Characterization of 12 Capsicum Varieties by Evaluation of Their Carotenoid Profile and Pungency Determination. Food Chemistry 2013, 140, 794–802.
  • Huang, X.F.; Xue, J.Y.; Jiang, A.Q.; Zhu, H.L. Capsaicin and Its Analogues: Structure-Activity Relationship Study. Current Medicinal Chemistry 2013, 20, 2661–2672.
  • Kaale, E.; Van Schepdael, A.; Roets, E.; Hoogmartens, J. Determination of Capsaicinoids in Topical Cream by Liquid-Liquid Extraction and Liquid Chromatography. Journal of Pharmaceutical and Biomedical Analysis 2002, 30, 1331–1337.
  • Barbero, G.F.; Liazid, L.; Azaroual, L.; Palma, M.; Barroso, C.G. Capsaicinoid Contents in Peppers and Pepper-Related Spicy Foods. International Journal of Food Properties 2016, 19, 485–493.
  • Surh, Y.J.; Lee, S.S. Capsaicin, a Double-Edged Sword: Toxicity, Metabolism, and Chemopreventive Potential. Life Sciences 1995, 56, 1845–1855.
  • Alvarez-Parrilla, E.; de la Rosa, L.A.; Amarowicz, R.; Shahidi, F. Antioxidant Activity of Fresh and Processed Jalapeno and Serrano Peppers. Journal of Agricultural and Food Chemistry 2011, 59, 163–173.
  • Bloomer, R.J.; Canale, R.E.; Shastri, S.; Suvarnapathki, S. Effect of Oral Intake of Capsaicinoid Beadlets on Catecholamine Secretion and Blood Markers of Lipolysis in Healthy Adults: A Randomized, Placebo Controlled, Double-Blind, Cross-Over Study. Lipids in Health and Disease 2010, 9, 72.
  • Spiller, F.; Alves, M.K.; Vieira, S.M.; Carvalho, T.A.; Leite, C.E.; Lunardelli, A.; Poloni, J.A.; Cunha, F.Q.; de Oliveira, J.R. Anti-Inflammatory Effects of Red Pepper (Capsicum Baccatum) on Carrageenan- and Antigen-Induced Inflammation. Journal of Pharmacy and Pharmacology 2008, 60, 473–478.
  • Suzuki, T.; Kawada, T.; Iwai, K. Formation and Metabolism of Pungent Principle of Capsicum Fruits VI. Effective Separation of Capsaicin and Its Analogs by Reversed-Phase High-Performance Thin-Layer Chromatography. Journal of Chromatography 1980, 198, 217–223.
  • Mueller-Seitz, E.; Hiepler, C.; Petz, M. Chili Pepper Fruits: Content and Pattern of Capsaicinoids in Single Fruits of Different Ages. Journal of Agricultural and Food Chemistry 2008, 56, 12114–12121.
  • Saha, S.; Walia, S.; Kundu, A.; Kaur, C.; Singh, J.; Sisodia, R. Capsaicinoids, Tocopherol, and Sterols Content in Chili (Capsicum sp.) by Gas Chromatographic-Mass Spectrometric Determination. International Journal of Food Properties 2015, 18, 1535–1545.
  • Barbero, G.F.; Liazid, A.; Palma, M.; Barroso, C.G. Fast Determination of Capsaicinoids from Peppers by High-Performance Liquid Chromatography Using a Reversed Phase Monolithic Column. Food Chemistry 2008, 107, 1276–1282.
  • Korkutata, N.F.; Kavaz, A. A Comparative Study of Ascorbic Acid and Capsaicinoid Contents in Red Hot Peppers (Capsicum Annum L.) Grown in Southeastern Anatolia Region. International Journal of Food Properties 2015, 18, 725–734.
  • Kozukue, N.; Han, J.S.; Kozukue, E.; Lee, S.J.; Kim, J.A.; Lee, K.R.; Levin, C.E.; Friedman, M. Analysis of Eight Capsaicinoids in Peppers and Pepper-Containing Foods by High-Performance Liquid Chromatography and Liquid Chromatography–Mass Spectrometry. Journal of Agricultural and Food Chemistry 2005, 53, 9172–9181.
  • Al Othman, Z.A.; Ahmed, Y.B.H.; Habila, M.A.; Ghafar, A.A. Determination of Capsaicin and Dihydrocapsaicin in Capsicum Fruit Samples Using High Performance Liquid Chromatography. Molecules 2011, 16, 8919–8929.
  • Chinn, M.S.; Sharma-Shivappa, R.R.; Cotter, J.L. Solvent Extraction and Quantification of Capsaicinoids from Capsicum Chinese. Food and Bioproducts Processing 2011, 89, 340–345.
  • Pauwels, J.; D’Autry, W.; Van den Bossche, L.; Dewever, C.; Forier, M.; Vandenwaeyenberg, S.; Wolfs, K.; Hoogmartens, J.; Van Schepdael, A.; Adams, E. Optimization and Validation of Liquid Chromatography and Headspace-Gas Chromatography Based Methods for the Quantitative Determination of Capsaicinoids, Salicylic Acid, Glycol Monosalicylate, Methyl Salicylate, Ethyl Salicylate, Camphor, and L-Menthol in a Topical Formulation. Journal of Pharmaceutical and Biomedical Analysis 2012, 60, 51–58.
  • Swartz, M.E. UPLC (TM): An Introduction and Review. Journal of Liquid Chromatography and Related Technologies 2005, 28, 1253–1263.
  • Ha, J.; Seo, H.-Y.; Shim, Y.-S.; Nam, H.-J.; Seog, H.; Ito, M.; Nakagawa, H. Rapid Method for the Determination of Capsaicin and Dihydrocapsaicin in Gochujang Using Ultra-High-Performance Liquid Chromatography. Journal of AOAC International 2010, 93, 1905–1911.
  • Alothman, Z.A.; Wabaidur, S.M.; Khan, M.R.; Ghafar, A.A.; Habila, M.A.; Ahmed, Y.B.H. Determination of Capsaicinoids in Capsicum Species Using Ultra Performance Liquid Chromatography–Mass Spectrometry. Journal of Separation Science 2012, 35, 2892–2896.
  • Barbero, G.F.; Liazid, A.; Palma, M.; Barroso, C.G. Ultrasound-Assisted Extraction of Capsaicinoids from Peppers. Talanta 2008, 75, 1332–1337.
  • Campana, A.M.G.; Rodriguez, L.C.; Barrero, F.A.; Ceba, M.R. ALAMIN: A Chemometric Program to Check Analytical Method Performance and to Assess the Trueness by Standard Addition Methodology. Trac-Trends in Analytical Chemistry 1997, 16, 381–385.
  • Attuquayefio, V.K.; Buckle, K.A. Rapid Sample Preparation Method for HPLC Analysis of Capsaicinoids in Capsicum Fruits and Oleoresins. Journal of Agricultural and Food Chemistry 1987, 35, 777–779.

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