2,453
Views
5
CrossRef citations to date
0
Altmetric
Original Articles

Identification and Functional Characterization of Cysteine Protease from Nine Pear Cultivars (Pyrus pyrifolia)

, , &
Pages 1631-1644 | Received 21 May 2015, Accepted 09 Oct 2015, Published online: 05 Apr 2016

REFERENCES

  • Choe, I.S.; Park, Y.J. A Study on Utilization As Meat Tenderizer from Korean Pear Protease. Korean Journal for Food Science of Animal Resources 1996, 16, 89–93.
  • Choe, I.S.; Park, Y.J.; Ishioroshi, M.; Samejima, K. A New Protease in Korean Pears As Meat Tenderizer. Animal Science Technology 1996, 67, 43–46.
  • Han, S.K.; Chin, K.B. Study on Meat Tenderness of a Protease Extracted from Domestic Pear. Korean Journal for Food Science of Animal Resources 2004, 24, 326–328.
  • Kumar, C.G.; Takagi, H. Microbial Alkaline Proteases: From a Bio-Industrial Viewpoint. Biotechnol Advances 1999, 17, 561–594.
  • Chaiwut, P.; Pintathong, P.; Rawdkuen, S. Extraction and Three-Phase Partitioning Behavior of Proteases from Papaya Peels. Process Biochemistry 2010, 45, 1172–1175.
  • Kee, H.J.; Hwang, Y.S.; Kim, J.H.; Hog, Y.H. Application Fig Protease to Foods. Korean Journal for Food Science of Animal Resources 1998, 18, 19–26.
  • Lee, D.H.; Jin, B.H. Encapsulation of Bromelain in Liposome. Journal of food Science and Nutrition 2001, 5, 81–85.
  • Karnchanatat, A.; Tiengburanatam, N.; Boonmee, A.; Puthong, S.; Sangvanich, P. Zingipain, a Cysteine Protease from Zingiber Ottensii Valetin Rhizomes with Antiproliferative Activities Against Fungi and Human Malignant Cell Lines. Preparative Biochemistry and Biotechnology 2011, 41, 138–153.
  • Nam, S.H.; Walsh, M.K.; Yang, K.Y. The Enzymatic Properties of Actinidin from Kiwifruit. Food Science and Biotechnology 2006, 15, 453–457.
  • Kim, E.M.; Choe, I.S.; Hwang, S.G. Effects of Singular Manner or Mixed Type Treatment of Proteases Isolated from Pear, Pineapple and Kiwifruit on Actomyosin Degradation. Korean Journal for Food Science of Animal Resources 2003, 23, 193–199.
  • Li, Q.H.; Mandai, P.K.; Lim, H.K.; Baatartsogt, Q.; Lee, C.H.; Jeon, G.J.; Choe, I.S.; Choi, K.D. Purification and Characterization of a Protease from Korean Pear (Pyrus Serotine L.) As Meat Tenderizer. Korean Journal for Food Science of Animal Resources 2009, 29, 157–163.
  • Bai, Y.H.; Roh, J.H. The Properties of Proteolytic Enzymes in Fruits (Pear, Kiwifruit, Fig, Pineapple, and Papaya). Korean Journal of Food and Cookery Science 2000, 16, 363–366.
  • Soares, P.A.G.; Vaz, A.F.M.; Correia, M.T.S.; Pessoa, A.; Carneiro-da-Cunha, M. Purification of Bromelain from Pineapple Wastes by Ethanol Precipitation. Separation and Purification Technology 2012, 98, 389–395.
  • Bradford, M.M. A Rapid and Sensitive Method for Quantification of Microgram Quantities of Proteins Utilizing the Principle of Protein Dye-Binding. Analytical Biochenistry 1976, 72, 248–254.
  • García-Carreño, F.L.; Dimes, L.E.; Haard, N. Substrate-Gel Electrophoresis for Composition and Molecular Weight of Proteinases of Proteinaceous Proteinase Inhibitor. Analytical. Biochemistry 1993, 214, 65–69.
  • Ha, M.; Bekhit, A.D.; Carne, A.; Hopkins, D.L. Characterization of Commercial Papain, Bromelain, Actinidin, and Zingibain Protease Preparations and Their Activities Toward Meat Proteins. Food Chemistry 2012, 134, 95–105.
  • Ha, M.; El-Din Bekhit, A.; Carnea, A.; Hopkins, D. Characterisation of Kiwifruit and Asparagus Enzyme Extracts, and Their Activities Toward Meat Proteins. Food Chemistry 2013, 136, 989–998.
  • Kang, S.U.; Lubec, G. Complete Sequencing of GABAA Receptor Subunit B3 by a Rapid Technique Following in-Gel Digestion of the Proteins. Electrophoresis 2009, 30, 2159–2167.
  • Chaiwut, P.; Nitsawang, S.; Shank, L.; Kanasawud, P. A Comparative Study on Properties and Proteolytic Components of Papaya Peel and Latex Proteases. Chiang Mai Journal of Science 2007, 34, 109–118.
  • Pastorello, E.A.; Conti, A.; Pravettoni, V.; Farioli, L.; Rivolta, F.; Ansaloni, R.; Ispano, M.; Incorvaia, C.; Giuffrida, G.G.; Ortolani, C. Identification of Actinidin As the Major Allergen of Kiwi Fruit. Journal of Allergy and Clinical Immunology 1998, 101, 531–537.
  • Napper, A.D.; Bennett, S.P.; Borowski, M.; Holdridge, M.B.; Leonard, M.J.; Rogers, E.E.; Duan, Y.; Laursen, R.A.; Reinhold, B.; Shames, S.L. Purification and Characterization of Multiple Forms of the Pineapple Stem-Derived Cysteine Proteinases Ananain and Comosain. Biochemical Journal 1994, 301, 727–735.
  • Rowan, A.D.; Buttlet, D.J.; Barrett, A.J. The Cysteine Proteinases of the Pineapple Plant. Biochemical Journal 1990, 266, 869–875.
  • Zare, H.; Moosavi-Movahedi, A.A.; Salami, M.; Mirzaei, M.; Saboury, A.A; Sheibanid, N. Purification and Autolysis of the Ficin Isoforms from Fig (Ficus Carica cv. Sabz) Latex. Phytochemistry 2013, 87, 16–22.
  • Devaraj, K.B.; Kumar, P.R.; Prakash, V. Purification, Characterization, and Solvent-Induced Thermal Stabilization of Ficin from Ficus Carica. Journal of Agricultural and Food Chemistry 2008, 56(23), 11417–11423.
  • Chalabi, M.; Khademi, F.; Yarani, R.; Mostafaie, A. Proteolytic Activities of Kiwifruit Actinidin (Actinidia Deliciosa cv. Hayward) on Different Fibrous and Globular Proteins: A Comparative Study of Actinidin with Papain. Applied Biochemistry and Biotechnology 2014, 172, 4025–4037.
  • Mehrnoush, A.; Norashikin, A.; Chuan, L.T.; Ping, T.C; Hamed, M. Optimisation of Serine Protease Extraction from Mango Peel (Mangifera Indica cv. Chokanan). Food Chemistry 2011, 124, 666–671.
  • Pickersgill, R.W.; Sumner, I.G.; Collins, M.E.; Goodenough, P.W. Structural and Electrostatic Differences Between Actinidin and Papain Account for Differences in Activity. Biochem J 1989, 257(1), 310–312.
  • Lima, C.A.; Freitas Jr., A.C.V.; Lima Filho, J.L.; Converti, A.; Vuana Marques, D.A.; Carneiro-da-Cunha, M.G.; Porto, A.L. Two-Phase Partitioning and Partial Characterization of a Collagenase from Penicillium Aurantiogriseum URM4622: Aplication to Collagen Hydrolysis. Biochemical Engineering Journal 2013, 75, 64–71.
  • Rowe, H.A.; Brown, M. Practical Enzyme Kinetics: A Biochemical Laboratory Experiment. Journal of Chemical Education 1988, 65, 548–549.
  • Chung, W.G.; Cristobal, L.; Claudia, M.; Maier, S. Detection of Carbonyl-Modified Proteins in Interfibrillar Rat Mitochondria Using N0-Aminooxymethylcarbonylhydrazino-Dbiotin As An Aldehyde/Keto-Reactive Probe in Combination with Western Blot Analysis and Tandem Mass Spectrometry. Electrophoresis 2008, 29, 1317–1324.
  • Arshad, Z.; Amid, A.; Yuso, F.; Jaswir, I.; Ahmad, K.; Loke, P. Bromelain: An Overview of Industrial Application and Purification Strategies. Applied Microbiology and Biotechnology 2014, 98, 7283–7297.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.