49
Views
29
CrossRef citations to date
0
Altmetric
Original Articles

New Diagnostic Tools for Neonatal Sepsis: The Role of a Real-Time Polymerase Chain Reaction for the Early Detection and Identification of Bacterial and Fungal Species in Blood Samples

, , , , , & show all
Pages 31-34 | Published online: 27 Oct 2016

References

  • Pazzi D, Klein J, Baker C. Bacterial sepsis and meningitis. In: Remington JS ed. Infectious diseases of the fetus and newborn infant, 6th edn. Philadelphia, PA: WB Saunders, 2006:248–95.
  • Fanaroff AA, Stoll BJ, Wright LL, Carlo WA, Ehrenkranz RA, Stark AR, et al. Trends in neonatal morbidity and mortality for very low birthweight infants. Am J Obstet Gynecol 2007;196(2):147.e1–8.
  • Benjamin DK Jr, Stoll BJ. Infection in late preterm infants. Clin Perinatol 2006;33(4):871–82.
  • Escobar GJ. The neonatal “sepsis work-up”: personal reflections on the development of an evidence-based approach toward newborn infections in a managed care organization. Pediatrics. 1999;103(Suppl. E):360–73.
  • Ng PC, Li K, Leung TF, Wong RP, Li G, Chui KM, et al. Early prediction of sepsis-induced disseminated intravascular coagulation with interleukin-10, interleukin-6, and RANTES in preterm infants. Clin Chem 2006;52(6):1181–9.
  • Malik A, Hui CPS, Pennie RA, Kirpalani H. Beyond the complete blood cell count & C-reactive protein: a systematic review of modern diagnostic tests for neonatal sepsis. Arch Pediatr Adolesc Med 2003;157:511–16.
  • Mishra UK, Jacobs SE, Doyle LW, Garland SM. Newer approaches to the diagnosis of early onset neonatal sepsis. Arch Dis Child Fetal Neonatal Ed 2006;91(3):F208–12.
  • Kurlat I, Stoll BJ, McGowan JE Jr. Time to positivity for detection of bacteremia in neonates. J Clin Microbiol 1989;27(5):1068–71.
  • Jardine L, Davies MW, Faoagali J. Incubation time required for neonatal blood cultures to become positive. J Paediatr Child Health 2006;42(12):797–802.
  • Connell TG, Rele M, Cowley D, Buttery JP, Curtis N. How reliable is a negative blood culture result? Volume of blood submitted for culture in routine practice in a children’s hospital. Pediatrics 2007;119(5):891–6.
  • Maiwald M. Broad-range PCR for the detection and identification of bacteria. In: Persing DH Ed. Molecular Microbiology: Diagnostic Principles and Practice. 2nd ed. Washington DC: ASM Press. 2003:379–90.
  • Golden SM, Stamilio DM, Faux BM, dela Cruz WP, Shoemaker CT, Blackmon CL, et al. Evaluation of a real-time fluorescent PCR assay for rapid detection of Group B Streptococci in neonatal blood. Diagn Microbiol Infect Dis 2004;50(1):7–13.
  • Yadav AK, Wilson CG, Prasad PL, Menon PK. Polymerase chain reaction in rapid diagnosis of neonatal sepsis. Indian Pediatr 2005;42(7):681–5.
  • Jordan JA, Durso MB. Real-time polymerase chain reaction for detecting bacterial DNA directly from blood of neonates being evaluated for sepsis. J Mol Diagn 2005;7(5):575–81.
  • Varani S., Stanzani M., Nardi L., Paolucci M., Vianelli N., Baccarani M., et al. Comparison of real-time PCR and blood culture for the diagnosis of bloodstream infections in onco-haematologic patients: microbiological and clinical assessment [Abstract]. Clin Microbiol Infect 2007;13:s393.
  • Raglio A, Rizzi M, Amer M, Mangia M, Lucà MG, Goglio A. Sepsis diagnosis by real-time PCR (SeptiFast kit, Roche Diagnostics): preliminary results and possible application [Abstract]. Clin Microbiol Infect 2006;12 (suppl.4):O215.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.