An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

References

• Cooper GM. The chemistry of cells: the central role of enzymes as biological catalysts. In: Cooper GM, editor. The cell: a molecular approach. 2nd ed. Sunderland (MA): Sinauer Associates; 2000. p. 145–146.
   Google Scholar
• Guisan JM. Immobilization of enzymes as the 21st century begins. In: Guisan JM, editor. Immobilization of enzymes and cells. 2nd ed. New Jersey (NJ): Humana Press Inc.; 2006. p. 1–13.
   Google Scholar
• Hernandez K, Fernandez-Lafuente R. Control of protein immobilization: coupling immobilization and side-directed mutagenesis to improve biocatalyst or biosensor performance. Enzyme Microb Technol. 2011;48:107–122.
   PubMed Web of Science ®Google Scholar
• Krajewska B. Chitin and its derivative as supports for immobilization of enzymes. Enzyme Microb Technol. 2004;35:26–39.
   Web of Science ®Google Scholar
• Sheldon RA. Cross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts. Biochem Soc. 2007;35(6):1583–1587.
   PubMed Web of Science ®Google Scholar
• Demetrius L. Thermodynamics and kinetics of protein folding an evolutionary perspective. J Theor Biol. 2002;217:397–411.
   PubMed Web of Science ®Google Scholar
• Wahab RA, Basri M, Abdul Rahman MB, Raja Abdul Rahman RNZ, Salleh AB, Leow TC. Manipulation of the conformation and enzymatic properties of T1 lipase by site-directed mutagenesis of the protein core. Appl Biochem Biotechnol. 2012;167:612–620.
   PubMed Web of Science ®Google Scholar
• Wahab RA, Basri M, Abdul Rahman MB, Raja Abdul Rahman RNZ, Salleh AB, Leow TC. Engineering catalytic efficiency of thermophilic lipase from Geobacillus zalihae by hydrophobic residue mutation near the catalytic pocket. Adv Biosci Biotechnol. 2012;3:158–167.
   Google Scholar
• Tosa T, Mori T, Fuse N, Chibata I. Studies on continuous enzyme reactions. I. Screening of carriers for preparation of water-insoluble aminoacylase. Enzymologia. 1966;31:214–224.
   PubMedGoogle Scholar
• Asuri P, Karajanagi SS, Sellitto E, Kim DY, Kane RS, Dordick JS. Water-soluble carbon nanotube-enzyme conjugates as functional biocatalytic formulations. Biotechnol Bioeng. 2006;95(5):804–811.
   Google Scholar
• Saifuddin N, Raziah AZ, Junizah AR. Carbon nanotubes: a review on structure and their interaction with proteins. J Chem. 2013; 2013:676815.
   Google Scholar
• Peng YE, Xu ZI, Kang WJ, Deng HT, Seta P. Covalent immobilization of lipase on poly(acrylonitrile-co-maleic acid) ultrafiltration hollow fiber membrane. Chem Rev Chin Univ. 2005;21(6):723–727.
   Web of Science ®Google Scholar
• Tian X, Anming W, Lifeng H, Haifeng L, Zhenming C, Qiuyan W, Xiaopu Y. Recent advance in the support and technology used in enzyme immobilization. Afr J Biotechnol. 2009;8(19):4724–4733.
   Web of Science ®Google Scholar
• Aehle W. Enzymes in industry. 3rd ed. Weinheim: Wiley-VCH; 2007.
   Google Scholar
• Cabral JMS, Kennedy JF. Immobilisation techniques for altering thermal stability of enzymes. In: Gupta MN, editor. Thermostability of enzymes. Berlin: Springer Verlag; 1993. p. 163–179.
   Google Scholar
• Liese A, Hilterhause L. Evaluation of immobilized enzymes for industrial applications. Chem Soc Rev. 2013;42:6236–6249.
   PubMed Web of Science ®Google Scholar
• Brena BM, Batista-Viera F. Immobilization of enzymes. In: Guisan JM, editor. Immobilization of enzymes and cells. 2nd ed. New Jersey (NJ): Humana Press Inc.; 2006. p. 123–124.
   Google Scholar
• Goel MK. 1994. Immobilized enzymes. [Internet]. [cited 2014 Apr]. Available from: http://www.rpi.edu/dept/chem-eng/Biotech-Environ/goel.html.
   Google Scholar
• Tischer W, Wedenkind F. Immobilized enzymes: methods and application. In: Fessner WD, editor. Topics in chemistry. Berlin: Springer Verlag; 1999. p. 100–108.
   Google Scholar
• Norouzian D. Enzyme immobilization: the state of art in biotechnology. Iran J Biotechnol. 2003;1(4):197–206.
   Google Scholar
• Brodelius P, Mosbach K. Immobilization techniques for cells/organelles. In: Mosbach K, editor. Methods in enzymology. London: Academic Press; 1987. p. 173–454.
   Google Scholar
• Buchholz K, Klein J. Characterization of immobilized biocatalysts. In: Mosbach K, editor. Methods in enzymology. London: Academic Press; 1987; p. 3–30.
   Google Scholar
• Li S, Hu J, Liu B. Use of chemically modified PMMA microspheres for enzyme immobilization. Biosystems. 2004;77:25–32.
   PubMed Web of Science ®Google Scholar
• Foresti ML, Ferreira ML. Chitosan-immobilized lipases for the catalysis of fatty acid esterifications. Enzyme Microb Technol. 2007;40:769–777.
   Web of Science ®Google Scholar
• Balcão VM, Paiva AL, Malcata FX. Bioreactors with immobilized lipases: state-of-the-art. Enzyme Microb Technol. 1996;18:392–416.
   PubMed Web of Science ®Google Scholar
• de Lathouder KM, van Benthem DTJ, Wallin SA, Mateo C, Fernandez LF, Guisan JM, Kapteijn F, Moulijn JA. Poliethyleneimine (PEI) functionalized ceramic monolithsas enzyme carriers: preparation and performance. J Mol Catal B. 2008;50:20–27.
   Web of Science ®Google Scholar
• Spahn C, Minteer SD. Enzyme immobilization in biotechnology. Recent Pat Eng. 2008;2:195–200.
   Google Scholar
• Khan AA, Alzohairy MA. Recent advances and applications of immobilized enzymes technologies: a review. Res J Biol Sci. 2010;5(8):565–575.
   Google Scholar
• Boller T, Meier C, Menzler S. Eupergit oxirane acrylic beads: how to make enzymes fit for biocatalysis. Org Process Res Dev. 2002;6(4):509–519.
   Web of Science ®Google Scholar
• Cao L, Langen L, Sheldon RA. Immobilised enzymes: carrier-bound or carrier-free? Curr Opin Biotechnol. 2003;14:387–394.
   PubMed Web of Science ®Google Scholar
• Pairat I, Thidarat C, Jitchon P, Navadon S, Jittima C. Application of agriculture waste as support for lipase immobilization. Biocatal Agric Biotechnol. 2014;3(3):77–82.
   Google Scholar
• Wu C, Zhou G, Jiang X, Ma J, Zhang H, Song H. Active biocatalysts based on Candida rugosa lipase immobilized in vesicular silica. Process Biochem. 2012;47:953–959.
   Web of Science ®Google Scholar
• Kim JB, Grate JW, Wang P. Nanostructures for enzyme stabilization. Chem Eng Sci. 2006;61:1017–1026.
   Web of Science ®Google Scholar
• Khan AA, Akhtar S, Hussain Q. Direct immobilization of polyphenol oxidases on celite 545 from ammonium sulphate fractionated proteins of potato (Solanum tuberosum). J Mol Catal B. 2006;40:58–63.
   Web of Science ®Google Scholar
• Ansari SA, Husain Q. Lactose hydrolysis from milk/whey in batch and continuous processes by concanavalin A-celite 545 immobilized Aspergillus oryzae β-galactosidase. Food Bioprod Process. 2012;90(2):351–359.
   Web of Science ®Google Scholar
• Datta S, Rene CL, Rajaram YRS. Enzyme immobilization: an overview on techniques and support materials. 3 Biotech. 2013;3(1):1–9.
   PubMedGoogle Scholar
• Gemenier P. Materials for enzyme engineering. In: Gemeiner P, editor. Enzyme engineering. 1st ed. New York (NY): Ellis Horwood; 1992. p. 13–119.
   Google Scholar
• Gorecka E, Jastrzebska M. Immobilization techniques and biopolymer carriers. Biotechnol Food Sci. 2011;75:65–86.
   Google Scholar
• Lee CH, Lin TS, Mou CY. Mesoporous materials for encapsulating enzymes. Nano. 2009;4:165–179.
   Web of Science ®Google Scholar
• Wang A, Wang H, Zhu S, Zhou C, Du Z, Shen S. An efficient immobilizing technique of penicillin acylase with combining mesocellular silicafoams support and p-benzoquinone cross linker. Bioprocess Biosyst Eng. 2008;31(5):509–517.
   PubMed Web of Science ®Google Scholar
• Guowei Z, Cuicui W, Xiaojie J, Jingyun M, Huayong Z, Hongbin S. Active biocatalysts based on Candida rugosa lipase immobilized in vesicular silica. Process Biochem. 2012;47:953–959.
   Web of Science ®Google Scholar
• Kim J, Jia H, Wang P. Challenges in biocatalysis for enzyme-based biofuel cells. Biotechnol Adv. 2006;24:296–308.
   PubMed Web of Science ®Google Scholar
• Wang L, Wei L, Chen Y, Jiang R. Specific and reversible immobilization of NADH oxidase on functionalized carbon nanotubes. J Biotechnol. 2010;150(1):57–63.
   PubMed Web of Science ®Google Scholar
• Verma ML, Naebe M, Barrow CJ, Puri M. Enzyme immobilisation on amino-functionalised multi-walled carbon nanotubes: structural and biocatalytic characterisation. PLOS One. 2013;8:1–9.
   Google Scholar
• Peijun J, Huishan T, Xin X, Wei F. Lipase covalently attached to multiwalled carbon nanotubes as an efficient catalyst in organic solvent. AIChE J. 2010;56:3005–3011.
   Web of Science ®Google Scholar
• Ansari SA, Husain Q. Potential applications of enzymes immobilized on/in nano materials: a review. Biotechnol Adv. 2012;30(3):512–523.
   PubMed Web of Science ®Google Scholar
• Chiou SH, Wu WT. Immobilization of Candida rugosa lipase on chitosan with activation of the hydroxyl groups. Biomaterials. 2004;25:197–204.
   PubMed Web of Science ®Google Scholar
• Costa SA, Azevedo HS, Reis RL. Enzyme immobilization in biodegradable polymers for biomedical applications. In: Reis RL, Román JS, editors. Biodegradable systems in tissue engineering and regenerative medicine. London: CRC Press LLC; 2005. p. 109–112.
   Google Scholar
• Flickinger MC, Drew SW. Fermentation, biocatalysis and bioseparation. In: Flickinger MC, editor. Encyclopedia of bioprocess technology. Vol. 1. 1st ed. New York (NY): Wiley; 1999.
   Google Scholar
• Jegannathan KR, Abang S, Poncelet D, Chan ES, Ravindra P. Production of biodiesel using immobilized lipase – a critical review. Crit Rev Biotechnol. 2008;28:253–264.
   PubMed Web of Science ®Google Scholar
• Kumar N. Studies of glucose oxidase immobilized carbon nanotube-polyaniline composites [MSc thesis]. Patiala (India): Thapar University; July 2009.
   Google Scholar
• Gupta M, Mattiason B. Determination of coupling yields and handling of labile proteins in immobilization technology. In: Taylor RF, editors. Protein immobilization. Fundamentals and applications. New York (NY): Marcel Dekker; 1992. p. 161–179.
   Google Scholar
• End N, Schöning KE. Immobilisation of biocatalyst in industrial research and production. Top Curr Chem. 2004;242:273–317.
   PubMed Web of Science ®Google Scholar
• Joshi KA, Tang J, Haddon R, Wang J, Chen W, Mulchandani A. A disposable biosensor for organophosphorus nerve agents based on carbon nanotubes modified thick film strip electrode. Electroanalysis. 2005;17(1):54–58.
   Web of Science ®Google Scholar
• Joshi KA, Prouza M, Kum M. V-type nerve agent detection using a carbon nanotube amperometric enzyme electrode. Anal Chem. 2006;78:331–336.
   PubMed Web of Science ®Google Scholar
• Cao L. Carrier-bound immobilized enzymes: principles, application and design. Weinheim: Wiley-VCH; 2005.
   Google Scholar
• Hanefield U, Gardossi L, Magner E. Understanding enzyme immobilisation. Chem Soc Rev. 2008;38:453–468.
   PubMed Web of Science ®Google Scholar
• Bahulekar R, Ayyangar NR, Ponrathnam S. Polyethyleneimine in immobilization of biocatalysts. Enzyme Microb Technol. 1991;13:858–868.
   PubMed Web of Science ®Google Scholar
• Nelson DL, Cox MM. Principles of biochemistry. 4th ed. New York (NY): W. H. Freeman and Co.; 2005.
   Google Scholar
• Roy I, Gupta MN. Bioaffinity immobilization. In: Guisan JM, editor. Immobilization of enzymes and cells. 2nd ed. New Jersey (NY): Humana Press Inc.; 2006. p. 189–192.
   Google Scholar
• Sardar M, Roy I, Gupta MN. Simultaneous purification and immobilization of Aspergillus niger xylanase on the reversibly soluble polymer Eudragit(TM) L-100. Enzyme Microb Technol. 2000;27:672–679.
   PubMed Web of Science ®Google Scholar
• Haider T, Husain Q. Concanavalin A layered calcium alginate-starch beads immobilized β-galactosidase as therapeutic agent for lactose intolerant patients. Int J Pharm. 2008;359:1–6.
   PubMed Web of Science ®Google Scholar
• Porath J. Salting out adsorption techniques for protein purifications. Biopolymers. 1987;26:S193–S204.
   PubMed Web of Science ®Google Scholar
• Huang L, Cheng ZM. Immobilization of lipase on chemically modified bimodal ceramic foams for olive oil hydrolysis. Chem Eng J. 2008;144:103–109.
   Web of Science ®Google Scholar
• Chronopoulou L, Kamel G, Sparago C, Bordi F, Lupi S. Structure-activity relationships of Candida rugosa lipase immobilised on polylactic acid nanoparticles. Soft Matter. 2011;7:2653–2662.
   Web of Science ®Google Scholar
• Bucur B, Danet AF, Marty JL. Versatile method of cholinesterase immobilisation via affinity bonds using concanavalin A applied to the construction of a screen-printed biosensor. Biosens Bioelectron. 2004;20:217–225.
   PubMed Web of Science ®Google Scholar
• Pryakhin AN, Chukhrai ES, Poltorak OM. Glucose 6-phosphate dehydrogenase immobilized by adsorption on silica gel solid supports. Vest Moskov Univ Ser 2 Khim. 1977;18(1):125.
   Google Scholar
• Kumakura M, Kaetsu I. Immobilization of cellulase using porous polymer matrix. J Appl Polym Sci. 2003;29(9):2713–2718.
   Web of Science ®Google Scholar
• Subramanian A, Kennel SJ, Oden PI, Jacobson KB, Woodward J, Doktycz MJ. Comparison of techniques for enzyme immobilisation on silicon supports – effect of cross-linker chain length on enzyme activity. Enzyme Microb Technol. 1999;24(1):26–34.
   Web of Science ®Google Scholar
• Chiang C-J, Hsiau L-T, Lee W-C. Immobilization of cell-associated enzymes by entrapment in polymethacrylamide beads. Biotechnol Technol. 2004;11(2):121–125.
   Web of Science ®Google Scholar
• Klotzbach TL, Watt MM, Ansari Y, Minteer SD. Improving the microenvironment for enzyme immobilization at electrodes by hydrophobically modifying chitosan and Nafion polymers. J Memb Sci. 2008;311:81–88.
   Web of Science ®Google Scholar
• Won K, Kim S, Kim KJ, Park HW, Moon SJ. Optimization of lipase entrapment in Ca-alginate gel beads. Process Biochem. 2005;40:2149–2154.
   Web of Science ®Google Scholar
• Shen Q, Yang R, Hua X, Ye F, Zhang W, Zhao W. Gelatin-templated biomimetic calcification for β-galactosidase immobilization. Process Biochem. 2011;46:1565–1571.
   Web of Science ®Google Scholar
• Keeling-Tucker T, Brennan JD. Fluorescent probes as reporters on the local structure and dynamics in sol-gel derived nanocomposite materials. Chem Mater. 2001;13:3331–3350.
   Web of Science ®Google Scholar
• Gill I. Bio-doped nanocomposite polymers: sol-gel bioencapsulates. Chem Mater. 2001;13:3404–3421.
   Web of Science ®Google Scholar
• Jin W, Brennan JD. Properties and applications of proteins encapsulated within sol-gel derived materials. Anal Chim Acta. 2002;461:1–36.
   Web of Science ®Google Scholar
• Tsai HC, Doong R. Preparation and characterization of urease-encapsulated biosensors in poly(vinyl alcohol)-modified silica sol-gel materials. Biosens Bioelectron. 2007;23:66–73.
   PubMed Web of Science ®Google Scholar
• Gao S, Wang Y, Diao X, Luo G, Dai Y. Effect of pore diameter and cross-linking method on the immobilization efficiency of Candida rugosa lipase in SBA-15. Bioresour Technol. 2010;101:3830–3837.
   PubMed Web of Science ®Google Scholar
• Lopez A, Lazaro N, Marques AM. The interphase technique: a simple method of cell immobilization in gel-beads. J Microbiol Methods. 1997;30:231–234.
   Web of Science ®Google Scholar
• Shi Y, Jin F, Wu Y, Yan F, Yu X, Quan Y. Improvement of immobilized cells through permealilizing and crosslinking. Chin J Biotechnol. 1997;13(1):111–113.
   Google Scholar
• Honda T, Miyazaki M, Nakamura H, Maeda H. Immobilization of enzymes on microchannel surface through cross-linking polymerization. AIChE Spring National Meeting; 2006 Apr 23–27; Orlando, FL.
   Google Scholar
• Sheldon RA. Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs). Appl Microbiol Biotechnol. 2011;92:467–477.
   PubMed Web of Science ®Google Scholar
• Migneault I, Dartiquenave C, Bertrand MJ, Waldron KC. Glutaraldehyde: behaviour in aqueous solution, reaction with proteins, and application to enzyme crosslinking. Biotechniques. 2004;37(5):790–802.
   PubMed Web of Science ®Google Scholar
• Obzturk B. Immobilization of lipase from Candida rugosa on hydrophobic and hydrophilic supports [MSc dissertation]. İzmir (Turkey): Izmir Institute of Technology; 2001. p. 40.
   Google Scholar
• Wu L, Yuan X, Sheng J. Immobilization of cellulase in nanofibrous PVA membranes by electrospinning. J Memb Sci. 2005;250:167–173.
   Web of Science ®Google Scholar
• Sakai S, Liu Y, Yamaguchi T, Watanabe R, Kawabe M, Kawakami K. Immobilization of Pseudomonas cepacia lipase onto electrospun polyacrylonitrile fibers through physical adsorption and application to transesterification in nonaqueous solvent. Biotechnol Lett. 2010;32:1059–1062.
   PubMed Web of Science ®Google Scholar
• Ren G, Xu X, Liu Q. Electrospun poly(vinyl alcohol)/ glucose oxidasebiocomposite membranes for biosensor applications. React Funct Polym. 2006;66:1559–1564.
   Web of Science ®Google Scholar
• Park JM, Kim M, Park HS, Min J, Kim YH. Immobilization of lysozyme-CLEA onto eletrospun chitosan nanofiber for effective antimicrobial applications. Int J Biol Macromol. 2013;54:37–43.
   PubMed Web of Science ®Google Scholar
• Singh BD. Biotechnology expanding horizonsa-amylase immobilization on the silica nanoparticles for cleaning performance towards starch soils in laundry detergents. J Mol Catal B. 2009;74:1–5.
   Google Scholar
• Dandavate V, Keharia H, Madamwar D. Ethyl isovelarate synthesis using Candida rugosa lipase immobilized on silica nanoparticles prepared in nonionic reverses micelles. Process Biochem. 2009;44:349–352.
   Web of Science ®Google Scholar
• Yilmaz E, Can K, Sezgin M, Yilmaz M. Immobilization of Candida rugosa lipase on glass bead for enantioselective hydrolysis of racemic naproxen methyl ester. Bioresour Technol. 2011;102:499–506.
   PubMed Web of Science ®Google Scholar
• Damnjanovíc J. Covalently immobilized lipase catalyzing high-yielding optimized geranyl butyrate synthesis in a batch and fluidized bed reactor. J Mol Catal B. 2012;75:50–59.
   Web of Science ®Google Scholar
• Ovsejevi K, Manta C, Battista-Viera F. Reversible covalent immobilization of enzymes via disulphide bonds. Methods Mol Biol. 2013;1051:89–116.
   PubMedGoogle Scholar
• Ispas C, Sokolov I, Andreescu S. Enzyme-functionalized mesoporous silica for bioanalytical applications. Anal Bioanal Chem. 2009;393:543–554.
   PubMed Web of Science ®Google Scholar
• Bing Z, Rui Z, Yazhen W, Congcong L, Jingtao W, Jindun L. Chitosan–halloysite hybrid nanotubes: horseradish peroxidase immobilization and applications in phenol removal. Chem Eng J. 2013;214:304–309.
   Web of Science ®Google Scholar
• Losic D, Short K, Gooding JJ, Shapter JG. Scanning probe microscopy characterisation of immobilised enzyme molecules on a biosensor surface: visualisation of individual molecules. J Serbian Chem Soc. 2004;69:93–106.
   Web of Science ®Google Scholar
• MacKenzie RC. Nomenclature of thermal analysis: part IV. Thermochim Acta. 1979;28:1–6.
   Web of Science ®Google Scholar
• Crompton TR. Polymer reference book. Shrewsbury: Rapra Publishing; 2006.
   Google Scholar
• Rajan A, Sudha JD, Abraham TE. Enzymatic modification of cassava starch by fungal lipase. Ind Crops Prod. 2008;27:50–59.
   Web of Science ®Google Scholar
• Kumar V, Yadav S, Jahan F, Saxena RK. Organic synthesis of maize starch-based polymer using Rhizopus oryzae lipase, scale up, and its characterization. Prep Biochem Biotechnol. 2014;44:321–331.
   PubMed Web of Science ®Google Scholar
• Blanco RM, Calvete JJ, Guisan JM. Immobilization-stabilization of enzymes. Variables that control the intensity of the trypsin (amine)- agarose (aldehyde) multi-point covalent attachment. Enzyme Microb Technol. 1988;11:353–359.
   Web of Science ®Google Scholar
• Yudianti R, Onggo H, Sudirman, Saito Y, Iwata T, Azuma JI. Analysis of functional group sited on multi-wall carbon nanotube surface. Open Mater Sci J. 2011;5:242–247.
   Google Scholar
• Romdhane IB, Fendri A, Gargouri Y, Gargouri A, Belghith H. A novel thermoactive and alkaline lipase from Talaromyces thermophilus fungus for use in laundry detergents. Biochem Eng J. 2010;53:112–120.
   Web of Science ®Google Scholar
• Chatterjeet S, Yadav D, Barbora L, Mahanta P, Goswami P. Silk-cocoon matrix immobilized lipase catalyzed transesteri fication of sunflower oil for production of biodiesel. J Catal. 2014;2014:1–7.
   Google Scholar
• Abdul Rahman MB, Zaidan UH, Basri M, Othman SS, Abdul Rahman RNZR, Salleh AB. Modification of natural feldspar as support for enzyme immobilization. J Nucl Relat Technol. 2009;6(1) 25–42.
   Google Scholar
• Wan L-S, Ke B-B, Wu J, Xu Z-K. Catalase immobilization on electrospun nanofibers: effects of porphyrin pendants and carbon nanotubes. J Phys Chem C. 2007;111:14091–14097.
   Web of Science ®Google Scholar
• Koster AJ, Ziese U, Verkleij AJ, Janssen AH, de Jong KP. Three-dimensional transmission electron microscopy: a novel imaging and characterization technique with nanometer scale resolution for materials science. J Phys Chem B. 2000;104:9368–9370.
   Web of Science ®Google Scholar
• Hayat MA. Positive staining for electron microscopy. New York (NY): Van Nostrand Reinhold Company; 1975.
   Google Scholar
• Noureddini H, Gao X. Characterization of sol-gel immobilized lipases. J Solgel Sci Technol. 2007;41:31–41.
   Web of Science ®Google Scholar
• Song C, Sheng L, Zhang X. Immobilization and characterization of a thermostable lipase. Mar Biotechnol. 2013;15:659–667.
   PubMed Web of Science ®Google Scholar
• Shah S, Solanki K, Gupta MN. Enhancement of lipase activity in non-aqueous media upon immobilisation on multi-walled carbon nanotubes. Chem Cent J. 2007;1:30.
   PubMed Web of Science ®Google Scholar
• Zhai R, Zhang B, Wan Y, Li C, Wang J, Liu J. Chitosan–halloysite hybrid-nanotubes: horseradish peroxidase immobilization and applications in phenol removal. Chem Eng J. 2013;214:304–309.
   Web of Science ®Google Scholar
• Liu X, Liu Y, Shen R, Liu H. Immobilization of lipase onto micron-sized magnetic bead. J Chromatogr B. 2005;822(1–2):1–7.
   PubMed Web of Science ®Google Scholar
• Bai S, Guo Z, Liu W, Sun Y. Resolution of (±)-menthol by immobilized Candida rugosa lipase on superparamagnetic nanoparticles. Food Chem. 2006;96:1–7.
   Web of Science ®Google Scholar
• Winograd N, Gaarenstroom SW. In: Kuwana T, editor. Physical methods in modern chemical analysis. Vol. 2. New York (NY): Academic Press; 1980. p. 117.
   Google Scholar
• Zhang YW, Prabhu P, Lee JK. Alginate immobilization of recombinant Escherichia coli whole cells harboring L-arabinose isomerase for L-ribulose production. Bioprocess Biosyst Eng. 2010;33:741–748.
   PubMed Web of Science ®Google Scholar
• Brundle CR, Conti G, Mack P. XPS and angle resolved XPS, in the semiconductor industry: characterization and metrology control of ultra-thin films. J Electron Spectrosc Relat Phenomena. 2010;178–179:433–448.
   Web of Science ®Google Scholar
• Gole JL. Direct synthesis of silicon nanowires, silica nanospheres, and wire-like nanospheres agglomerates. Appl Phys Lett. 2000;76:2346.
   Web of Science ®Google Scholar
• Chusuei CC, Goodman DW. X-ray photoelectron spectroscopy. In: Meyers RA, editor. Encyclopedia of physical science and technology. Vol. 17. 3rd ed. New York (NY): Academic Press; 2002. p. 921–938.
   Google Scholar
• Ye D-X, Pimanpang S, Jezewski C, Tang F, Senkevich JJ, Wang G-G, Lu T-M. Low temperature vapor chemical deposition of Co thin films from Co2(CO)8. Thin Solid Films. 2005;485:95–100.
   Web of Science ®Google Scholar
• Al-Bataineh SA, Britcher LG, Griesser HJ. Rapid radiation degradation in the XPS analysis of antibacterial coatings of brominated furanones. Surf Interface Anal. 2006;38:1512–1518.
   Web of Science ®Google Scholar
• Moulder JF, Stickle WF, Sobol PE, Bomben KD. Handbook of x-ray photoelectron spectroscopy. Eden Prairie (MN): Perkin-Elmer Corp; 1992.
   Google Scholar
• Sonnichsen C, Reinhard BM, Liphardt J, Alivisatos APA. Molecular ruler based on plasmon coupling of single gold and silver nanoparticles. Nat Biotechnol. 2005;23:741–745.
   PubMed Web of Science ®Google Scholar
• Schuck P, McPhee CE, Howlett GJ. Determination of sedimentation coefficients for small peptides. Biophys J. 1998;74:466–474.
   PubMed Web of Science ®Google Scholar
• Jung M, Hubert DHW, van Veldhoven E, Frederik PM, Blandamer MJ, Briggs B, Visser AJWG, van Herk AM, German AL. Interaction of styrene with DODAB bilayer vesicles. Influence on vesicle morphology and bilayer properties. Langmuir. 2000;16(3):968–979.
   Web of Science ®Google Scholar
• Zhao J, Das A, Schatz GC, Sligar ST, Van Duyne RP. Resonance localized surface plasmon spectroscopy: sensing substrate and inhibitor binding to cytochrome P450. J Phys Chem. 2008;112:13084–13088.
   Web of Science ®Google Scholar
• Claridge SA, Schwartz JJ, Weiss PS. Electrons, photons, and force: quantitative single-molecule measurements from physics to biology. ACS Nano. 2011;5:693–729.
   PubMed Web of Science ®Google Scholar
• Ribeiro Jr EA, Ramos CHI. Circular permutation and deletion studies of myoglobin indicate that the correct position of its N terminus is required for native stability and solubility but not for native-like heme binding and folding. Biochemistry. 2005;44:4699–4709.
   PubMed Web of Science ®Google Scholar
• Balan A, Santa-Cruz CP, Moutran A, Ferreira RCC, Medrano FJ, Pérez CA, Ramos CHI, Ferreira LCS. The molybdate-binding protein (ModA) of the phytopathogen Xanthomonas axonopodis pv citri. Protein Expr Purif. 2006;50:215–222.
   PubMed Web of Science ®Google Scholar
• Regis WCB, Fattori J, Santoro MM, Jamin M, Ramos CHI. On the difference in stability between horse and sperm whale myoglobins. Arch Biochem Biophys. 2005;436:168–177.
   PubMed Web of Science ®Google Scholar
• Correa DHA, Ramos CHI. The use of circular dichroism spectroscopy to study protein folding, form and function. Afr J Biochem Res. 2009;3:164–173.
   Google Scholar
• Kelly SM, Price NC. Circular dichroism to study protein interactions. Current Protocols in Protein Science. 2006. Chapter 20, Unit 20.10. New York (NY): Wiley Interscience.
   Google Scholar
• Cary PD, Kneale GG. Circular dichroism for the analysis of protein-DNA interactions. Methods Mol Biol. 2009;543:613–624.
   PubMedGoogle Scholar
• Mittal S, Singh LR. Denatured state structural property determines protein stabilization by macromolecular crowding: a thermodynamic and structural approach. PLOS One. 2013;8(11):e78936.
   PubMed Web of Science ®Google Scholar
• Greenfield NJ. Analysis of the kinetics of folding of protein and peptides using circular dichroism. Nat Protoc. 2006;1:2891–2899.
   PubMed Web of Science ®Google Scholar
• Binning G, Quate CF, Gerber C. Atomic force microscope. Phys Rev Lett. 1986;56:930–933.
   PubMed Web of Science ®Google Scholar
• Marcuello C, de Miguel R, Gómez-Moreno C, Martínez-Júlvez M, Lostao A. An efficient method for enzyme immobilization evidenced by atomic force microscopy. Protein Eng Des Sel. 2012;25:715–723.
   PubMed Web of Science ®Google Scholar
• Merkel R, Nassoy P, Leung A, Ritchie K, Evans E. Energy landscapes of receptor-ligand bonds explored with dynamic force spectroscopy. Nature. 1999;497:50–53.
   Google Scholar
• Yan XD, Xu XK, Ji H-F. Detection of femtomolar concentrations of HF using an SiO2 microcantilever. Anal Chem. 2005;77:6197–6204.
   PubMed Web of Science ®Google Scholar
• De Pablo P, Colchero J, Gomez-Herrero J, Baro´ AM. Jumping mode scanning force microscopy. Appl Phys Lett. 1998;73:3300–3302.
   Web of Science ®Google Scholar
• Sotres J, Lostao A, Wildling L, Ebner A, Gomez-Moreno C, Gruber HJ, Hinterdorfer P, Baro´ AM. Unbinding molecular recognition force maps of localized single receptor molecules by atomic force microscopy. Chem Phys Chem. 2008;9:590–599.
   Google Scholar
• Zhang P, Tan W. Atomic force microscopy for the characterization of immobilized enzyme molecules on biosensor surfaces. Fresenius J Anal Chem. 2001;369:302–307.
   PubMedGoogle Scholar
• Wadso I. Microcalorimetric techniques for characterization of living cellular systems. Will there be any important practical applications? Thermochim Acta. 1995;269/270:337–350.
   Web of Science ®Google Scholar
• Battistel E, Rialdi G. Characterization of immobilized enzymes by microcalorimetry. Methods Biotechnol. 2006;22:295–310.
   Google Scholar
• Russel M, Chen H, Yao J, Wang F, Zhou Y, Choi MMF, Zaray G, Trebse P. Different technique of calorimetry and their applications to environmental sciences: a review. JASA. 2009;5:194–208.
   Google Scholar
• Prashant P. Application of microcalorimeter in stability study [Internet]. Anand (India): Department of Pharmaceutical Technology, Indukaka Ipcowala College of Pharmacy; c2012 [cited 2013 Feb 12]. Available from: http://www.slideshare.net/pr-prashantpatel/application-of-microcalorimeter-in-stability-study.
   Google Scholar
• Griko YV, Makhatadze GI, Privalov PL, Hartley RW. Thermodynamics of barnase unfolding. Protein Sci. 1994;3:669–676.
   PubMed Web of Science ®Google Scholar
• Wintrode PL, Makhatadze GI, Privalov PL. Thermodynamics of ubiquitin unfolding. Proteins. 1994;18:246–253.
   PubMed Web of Science ®Google Scholar
• Stryer L. Fluorescence energy transfer as a spectroscopic ruler. Annu Rev Biochem. 1978;47:819–846.
   PubMed Web of Science ®Google Scholar
• Lakowicz JR. Energy transfer. In: Feng J, editor. Principles of fluorescence spectroscopy. 2nd ed. New York (NY): Wiley Plenum Press; 1983. p. 367–394.
   Google Scholar
• Berney C, Danuser G. FRET or no FRET: a quantitative comparison. Biophys J. 2003;84:3992–4010.
   PubMed Web of Science ®Google Scholar
• Cardullo RA. Principles of non-radiative FRET: the spectroscopic ruler. Microsc Anal. 2002;15:19–21.
   Google Scholar
• Cardullo RA. Theoretical principles and practical considerations for fluorescence resonance energy by transfer microscopy. Methods Cell Biol. 2013;114:441–456.
   PubMed Web of Science ®Google Scholar
• Stryer L, Haugland RP. Energy transfer – a spectroscopic ruler. Proc Natl Acad Sci USA. 1967;58:719–726.
   PubMed Web of Science ®Google Scholar
• Hussain SA. An introduction to fluorescence resonance energy transfer (FRET). Sci J Phys. 2012;2012:4.
   Google Scholar
• Tetin S. Fluorescence fluctuation spectroscopy (FFS). Part B. In: Tetin S, editor. Methods in enzymology. Vol 519. Amsterdam: Academic Press, Elsevier; 2013.
   Google Scholar
• Kenworthy AK. Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy. Methods. 2001;24:289–296.
   PubMed Web of Science ®Google Scholar
• Xu X, Brzostowski JA, Jin T. Using quantitative fluorescence microscopy and FRET imaging to measure spatiotemporal signaling events in single living cells. Methods Mol Biol. 2006;346:281–296.
   PubMedGoogle Scholar
• Nienhaus GU. Exploring protein structure and dynamics under denaturing conditions by single-molecule FRET analysis. Macromol Biosci. 2006;6:907–922.
   PubMed Web of Science ®Google Scholar
• Elangovan M, Day RN, Periasamy A. Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell. J Microsci. 2002;205:3–14.
   PubMed Web of Science ®Google Scholar
• Giepmans BNG, Adams SR, Ellisman MH, Tsien RY. The fluorescent toolbox for assessing protein location and function. Science. 2006;312:217–224.
   PubMed Web of Science ®Google Scholar
• Souto RM, Lamaka SV, González S. Uses of scanning electrochemical microscopy in corrosion research. Microsc Sci Technol Appl Educ. 2010;3:1769–1780.
   Google Scholar
• Amemiya S, Bard AJ, Fan FRF, Mirkin MV, Unwin PR. Scanning electrochemical microscopy. Annu Rev Anal Chem. 2008;1:95–131.
   PubMed Web of Science ®Google Scholar
• Lu X, Wang Q, Liu X. Review: recent applications of scanning electrochemical microscopy to the study of charge transfer kinetics. Anal Chim Acta. 2007;601:10–25.
   PubMed Web of Science ®Google Scholar
• Mirkin MV, Horrocks BR. Electroanalytical measurements using the scanning electrochemical microscope. Anal Chim Acta. 2000;406:119–146.
   Web of Science ®Google Scholar
• Pust SE, Maier W, Wittstock G. Investigation of localized catalytic and electrocatalytic processes and corrosion reactions with scanning electrochemical microscopy (SECM). Zeitschrift für Physikalische Chemie. 2008;222:1463–1517.
   Web of Science ®Google Scholar
• Sun P, Laforge FO, Mirkin MV. Scanning electrochemical microscopy in the 21st century. Phys Chem Chem Phys. 2007;9:802–823.
   PubMed Web of Science ®Google Scholar
• Stoica L, Neugebauer S, Schuhmann W. Scanning electrochemical microscopy (SECM) as a tool in biosensor research. Adv Biochem Eng Biotechnol. 2008;109:455–492.
   PubMed Web of Science ®Google Scholar
• Edwards MA, Martin S, Whitworth AL, Macpherson JV, Unwin PR. Scanning electrochemical microscopy: principles and applications to biophysical systems. Physiol Meas. 2006;27:R63–R108.
   PubMed Web of Science ®Google Scholar
• Noël JM, Zigah D, Simonet J, Hapiot P. Synthesis and immobilization of Ag(0) nanoparticles on diazonium modified electrodes: SECM and cyclic voltammetry studies of the modified interfaces. Langmuir. 2010;26(10):7638–7643.
   PubMed Web of Science ®Google Scholar
• Zigah D, Pellissier M, Fabre B, Barrière F, Hapiot P. Covalent immobilization and SECM analysis in feedback mode of glucose oxidase on a modified oxidized silicon surface. J Electroanal Chem. 2008;628:144–147.
   Web of Science ®Google Scholar
• Bard AJ, Fan F-RF, Pierce DT, Unwin PR, Wipf DO, Zhou F. Chemical imaging of surfaces with the scanning electrochemical microscope. Science. 1991;254:68–74.
   PubMed Web of Science ®Google Scholar
• Pyo M, Bard AJ. Scanning electrochemical microscopy: determination of diffusion coefficients and concentrations of Ru(NH3)(6)(3+) and methylene blue in polyacrylamide films by chronoamperometry at ultramicrodisk electrodes. Electrochim Acta. 1997;42:3077–3083.
   Web of Science ®Google Scholar
• Liu B, Mirkin MV. Charge transfer reactions at the liquid/liquid interface. Anal Chem. 2001;73:670A–677A.
   PubMed Web of Science ®Google Scholar
• Pierce DT, Unwin PR, Bard AJ. Scanning electrochemical microscopy. XVII. Studies of enzyme-mediator kinetics for membrane-and-surface-immobilised glucose oxidase. Anal Chem. 1992;64:1795–1804.
   Web of Science ®Google Scholar
• Wittstock G, Yu K-J, Halsall HB, Ridgeway TH, Heineman WR. Imaging of immobilized antibody layers with scanning electrochemical microscopy. Anal Chem. 1995;67:3578–3582.
   PubMed Web of Science ®Google Scholar
• Shiku H, Matsue T, Uchida I. Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy. Anal Chem. 1996;68:1276–1278.
   PubMed Web of Science ®Google Scholar
• Liu B, Rotenberg SA, Mirkin MV. Scanning electrochemical microscopy of living cells: different redox activities of nonmetastatic and metastatic human breast cells. Proc Natl Acad Sci USA. 2000;97:9855–9860.
   PubMed Web of Science ®Google Scholar
• Cai CX, Liu B, Mirkin MV, Frank HA, Rusling JF. Scanning electrochemical microscopy of living cells: Rhodobacter sphaeroide. Anal Chem. 2002;74:114–119.
   PubMed Web of Science ®Google Scholar
• Fan FRF, Bard AJ. Imaging of biological macromolecules on mica in humid air by scanning electrochemical microscopy. Proc Natl Acad Sci USA. 1999;96:14222–14227.
   PubMed Web of Science ®Google Scholar
• Wang J, Zhou F. Scanning electrochemical microscopic imaging of surface-confined DNA probes and their hybridization via guanine oxidation. J Electroanal Chem. 2002;537:95–102.
   Web of Science ®Google Scholar
• Yamashita K Takagi M, Uchida K, Kondo H, Takenaka K. Visualization of DNA microarrays by scanning electrochemical microscopy. Analyst. 2001;126:1210–1211.
   PubMed Web of Science ®Google Scholar
• Freeman M. Proteins at solid-liquid interfaces. Heidelberg: Springer Verlag; 2006.
   Google Scholar
• Belu AM, Graham DJ, Castner DG. Time-of-flight secondary ion mass spectrometry: techniques and applications for the characterization of biomaterial surfaces. Biomaterials. 2003;24:3635–3653.
   PubMed Web of Science ®Google Scholar
• Adriaens A, Van Vaeck L, Adams F. Static secondary ion mass spectrometry (S-SIMS) part 2: material science applications. Mass Spectrom Rev. 1999;18:48–81.
   Web of Science ®Google Scholar
• Zheng L, McQuaw CM, Baker MJ, Lockyer NP, Vickerman JC, Ewing AG, Winograd N. Investigating lipid-lipid and lipid-protein interactions in model membranes by TOF-SIMS. Appl Sci Surf. 2008;255:1190–1192.
   PubMed Web of Science ®Google Scholar
• Johansson P. Characterization of protein surface interactions: collagen and osteocalcin [master's thesis]. Sweden: Linköping University; 2013.
   Google Scholar
• Thiel V, Sjövall P. Using time-of-flight secondary ion mass spectrometry to study biomarkers. Rev Adv. 2011;39:125–156.
   Google Scholar
• Trzcinska R, Piotr S, Bodzon-Kulakowska A, Skalska M, Andrzej M, Kubacki J, Pedrys R, Silberrin J, Dworak A, Trzebicka B. Synthesis and characterisation of PEG-peptide surfaces for proteolytic enzyme detection. Anal Bioanal Chem. 2013;405:9049–9059.
   PubMed Web of Science ®Google Scholar
• Benninghoven A. Chemical analysis of inorganic and organic surfaces and thin films by static time-of-flight secondary ion mass spectrometry (TOF-SIMS). Angewandte Chemie Int (in English). 1994;33(10):1023–1043.
   Web of Science ®Google Scholar
• Lee J, Ceglia A, Kim K-J, Lee Y-H. Characterization of dyed textiles using TOF-SIMS and FT-IR. Surf Interface Anal. 2012;6:653–657.
   Web of Science ®Google Scholar
• Baio JE, Weidner T, Interlandi G, Mendoza-Barrera C, Canavan HE, Michel R, Castner DG. Probing albumin adsorption onto calcium phosphates by x-ray photoelectron spectroscopy and time-of-flight secondary ion mass spectrometry. J Vacuum Sci Technol B [Internet]. 2011 [cited 2013 March 5];29:04D113. Available from: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3260791/.
   PubMed Web of Science ®Google Scholar