Advanced search
Publication Cover
Biotechnology & Biotechnological Equipment Volume 30, 2016 - Issue 5
Submit an article Journal homepage
1,305
Views
3
CrossRef citations to date
0
Altmetric
Articles; Agriculture and Environmental Biotechnology

Rapid in vitro splicing of coding sequences from genomic DNA by isothermal recombination reaction-based PCR

Wenxuan ChenState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou,China;Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou, China;College of Life Sciences, South China Agricultural University, Guangzhou, ChinaView further author information
,
Dongchang ZengState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou,China;Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou, China;College of Life Sciences, South China Agricultural University, Guangzhou, ChinaView further author information
,
Rongxin ShenState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou,China;College of Life Sciences, South China Agricultural University, Guangzhou, ChinaView further author information
,
Xingliang MaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou,China;College of Life Sciences, South China Agricultural University, Guangzhou, ChinaView further author information
,
Qunyu ZhangState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou,China;Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou, China;College of Life Sciences, South China Agricultural University, Guangzhou, ChinaView further author information
,
Letian ChenState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou,China;Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou, China;College of Life Sciences, South China Agricultural University, Guangzhou, China;Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms,Guangzhou, ChinaView further author information
,
Yao-Guang LiuState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou,China;Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou, China;College of Life Sciences, South China Agricultural University, Guangzhou, ChinaCorrespondence[email protected]
View further author information
&
Qinlong ZhuState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou,China;Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou, China;College of Life Sciences, South China Agricultural University, Guangzhou, ChinaCorrespondence[email protected]
View further author information
 show all
Pages 864-868 | Received 12 Dec 2015, Accepted 16 May 2016, Published online: 16 Jun 2016

References

  • Vandenbroeck K, Dijkmans R, van Aerschot A, et al. Engineering by PCR-based exon amplification of the genomic porcine interferon-gamma DNA for expression in Escherichia coli. Biochem Biophys Res Commun. 1991;180:1408–1415.
  • An X, Lu J, Huang JD, et al. Rapid assembly of multiple-exon cDNA directly from genomic DNA. PLoS One. 2007;2:e1179.
  • Davies WL, Carvalho LS, Hunt DM. SPLICE: a technique for generating in vitro spliced coding sequences from genomic DNA. Biotechniques. 2007;43:785–789.
  • Zhou L, Lin Q, Zhang J, et al. A rapid DNA assembling strategy mediated by direct full-length polymerase chain reaction. Gene. 2013;523:122–125.
  • Gibson DG, Young L, Chuang RY, et al. Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods. 2009;6:343–345.
  • Gibson DG, Smith HO, Hutchison CR, et al. Chemical synthesis of the mouse mitochondrial genome. Nat Methods. 2010;7:901–903.
  • Gibson DG. Enzymatic assembly of overlapping DNA fragments. Method Enzymol. 2011;498:349–361.
  • Jiang Y, Xie M, Zhu Q, et al. One-step cloning of intron-containing hairpin RNA constructs for RNA interference via isothermal in vitro recombination system. Planta. 2013;238:325–330.
  • Zhu Q, Yang Z, Zhang Q, et al. Robust multi-type plasmid modifications based on isothermal in vitro recombination. Gene. 2014;548:39–42.
  • Niu B, He F, He M, et al. The ATP-binding cassette transporter OsABCG15 is required for anther development and pollen fertility in rice. J Integra Plant Biol. 2013;55:710–720.
  • Wang H, Chu Z, Ma X, et al. A high through-put protocol of plant genomic DNA preparation for PCR. Acta Agronomica Sinica. 2013;39:1200–1205.
  • Chen L, Wang F, Wang X, et al. Robust one-tube Ω-PCR strategy accelerates precise sequence modification of plasmids for functional genomics. Plant Cell Physiol. 2013;54:634–642.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.