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Biotechnology & Biotechnological Equipment Volume 32, 2018 - Issue 2
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Original Articles

A directed self-assembly quasi-spider silk protein expressed in Pichia pastoris

Bin LiuDepartment of Medicine, Jinggangshan University, Jian, P.R. ChinaORCID Iconhttp://orcid.org/0000-0001-7115-913XView further author information
ORCID Icon,
Tao WangDepartment of Medicine, Jinggangshan University, Jian, P.R. ChinaORCID Iconhttp://orcid.org/0000-0003-4467-945XView further author information
ORCID Icon,
Liyan XiaoSchool of Foreign Languages, Jinggangshan University, Jian, P.R. ChinaORCID Iconhttp://orcid.org/0000-0003-4415-7192View further author information
ORCID Icon,
Guilan ZhangDepartment of Medicine, Jinggangshan University, Jian, P.R. ChinaORCID Iconhttp://orcid.org/0000-0003-3340-2623View further author information
ORCID Icon,
Guangshen LiDepartment of Medicine, Jinggangshan University, Jian, P.R. ChinaORCID Iconhttp://orcid.org/0000-0002-8023-6552View further author information
ORCID Icon,
Jingzhi LuoDepartment of Medicine, Jinggangshan University, Jian, P.R. ChinaORCID Iconhttp://orcid.org/0000-0003-2540-0441View further author information
ORCID Icon &
Xiaobing LiuSchool of Chemistry and Chemical Engineering, Jinggangshan University, Jian, P.R. ChinaCorrespondence[email protected] [email protected]
ORCID Iconhttp://orcid.org/0000-0001-7623-0804View further author information
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Pages 451-461 | Received 16 Feb 2017, Accepted 04 May 2017, Published online: 11 May 2017

References

  • Bluem C, Nichtl A, Scheibel T. Spider silk capsules as protective reaction containers for enzymes. Adv Funct Mater. 2014;24(6):763–768.
  • Bittencourt DMDC. Spider silks and their biotechnological applications. Short Views Insect Genomics Proteomics. 2016;4:211–227.
  • Hinman MB, Teul é F, Perry D, et al. Modular spider silk fibers: defining new modules and optimizing fiber properties. In: Asakura T, Miller T, editors. Biotechnology of silk. Vol. 5. Netherlands: Springer; 2014. p. 137–164. ( Biologically-Inspired Systems).
  • Chiasson R, Hasan M, Nazer Q A, et al. The use of silk in nanomedicine applications. In: Howard KA, Vorup-Jensen T, Peer D, editors. Nanomedicine. New York (NY): Springer; 2016. p. 245–278.
  • Thatikonda N. Functionalization of partial spider silk with affinity domains and its use for diverse applications [licentiate thesis]. Uppsala: Sveriges lantbruksuniv; 2016.
  • Chung H, Kim TY, Sang YL. Recent advances in production of recombinant spider silk proteins. Curr Opin Biotech. 2012;23(6):957–964.
  • Xia XX, Demain AL. Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber. Proc Natl Acad Sci USA. 2010;107(32):14059–14063.
  • Weichert N, Hauptmann V, Menzel M, et al. Transglutamination allows production and characterization of native-sized ELPylated spider silk proteins from transgenic plants. Plant Biotechnol J. 2014;12(2):265–275.
  • Peng CA, Russo J, Gravgaard C, et al. Spider silk-like proteins derived from transgenic Nicotiana tabacum. Transgenic Res. 2016;25(4):1–10.
  • Teulé F, Miao YG, Sohn BH, et al. Silkworms transformed with chimeric silkworm/spider silk genes spin composite silk fibers with improved mechanical properties. Proc Natl Acad Sci USA. 2012;109(3):923–928.
  • Weichert N, Hauptmann V, Helmold C, et al. Seed-specific expression of spider silk protein multimers causes long-term stability. Front Plant Sci. 2016 [ cited 2016 Jan 28];7:6. DOI:10.3389/fpls.2016.00006
  • Jansson R, Lau CH, Ishida T, et al. Functionalized silk assembled from a recombinant spider silk fusion protein (Z-4RepCT) produced in the methylotrophic yeast Pichia pastoris. J Biotechnol. 2016;11(5):687–699.
  • An B, Hinman MB, Holland GP, et al. Inducing β;-sheets formation in synthetic spider silk fibers by aqueous post-spin stretching. Biomacromolecules. 2011;12(6):2375–2381.
  • Gómez-Guillén MC, Giménez B, López-Caballero ME, et al. Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocolloid. 2011;25(8):1813–1827.
  • Ramshaw JAM. Biomedical applications of collagens. J Biomed Mater Res B. 2015;172(1):7–11.
  • Werten MW, Teles H, Moers AP, et al. Precision gels from collagen-inspired triblock copolymers. Biomacromolecules. 2009;10(5):1106–1113.
  • Butkowski RJ, Noelken ME, Hudson BG. Estimation of the size of collagenous proteins by electrophoresis and gel chromatography. Method Enzymol. 1982;82C:410–423.
  • Werten MWT, Bosch TJVD, Wind RD, et al. High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast. 1999;15(11):1087–1096.
  • Pourcq KD, Schutter KD, Callewaert N. Engineering of glycosylation in yeast and other fungi: current state and perspectives. Appl Microbiol Biot. 2010;87(5):1617–1631.
  • Iglesias D, Bosi S, Melchionna M, et al. The glitter of carbon nanostructures in hybrid/composite hydrogels for medicinal use. Curr Top Med Chem. 2016;16(18):1976–1989.
  • Grip S, Johansson J, Hedhammar M. Engineered disulfides improve mechanical properties of recombinant spider silk. Protein Sci. 2009;18(5):1012–1022.
  • Cranford SW. Increasing silk fibre strength through heterogeneity of bundled fibrils. J R Soc Interface. 2013 [ cited 2013 May 6];10(82):20130148. DOI:10.1098/rsif.2013.0148
  • Fahnestock SR, Bedzyk LA. Production of synthetic spider dragline silk protein in Pichia pastoris. Appl Microbiol Biot. 1997;47(1):33–39.

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