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Research Article

Characterization and applications of nanobodies against Pseudomonas aeruginosa Exotoxin A selected from single alpaca B cells

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Pages 1028-1037 | Received 21 May 2020, Accepted 27 Aug 2020, Published online: 08 Sep 2020

References

  • Muyldermans S. Nanobodies: natural single-domain antibodies. Annu Rev Biochem. 2013;82:775–797.
  • Desmyter A, Transue TR, Ghahroudi MA, et al. Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme. Nat Struct Biol. 1996;3(9):803–811.
  • Muyldermans S, Cambillau C, Wyns L. Recognition of antigens by single-domain antibody fragments: the superfluous luxury of paired domains. Trends Biotechnol. 2001;26(4):230–235.
  • Muyldermans S. Single domain camel antibodies: current status. Trends Biotechnol. 2001;74(4):277–302.
  • De Meyer T, Muyldermans S, Depicker A. Nanobody-based products as research and diagnostic tools. Trends Biotechnol. 2014;32(5):263–270.
  • Saccodossi N, De Simone EA, Leoni J. Structural analysis of effector functions related motifs, complement activation and hemagglutinating activities in Lama glama heavy chain antibodies. Vet Immunol Immunopathol. 2012;145(1–2):323–331.
  • Li G, Zhu M, Ma L, et al. Generation of small single domain nanobody binders for sensitive detection of testosterone by electrochemical impedance spectroscopy. ACS Appl Mater Interfaces. 2016;8(22):13830–13838.
  • Dufner P, Jermutus L, Minter RR. Harnessing phage and ribosome display for antibody optimisation. Trends Biotechnol. 2006;24(11):523–529.
  • Pellis M, Pardon E, Zolghadr K, et al. A bacterial-two-hybrid selection system for one-step isolation of intracellularly functional nanobodies. Arch Biochem Biophys. 2012;526(2):114–123.
  • Tiller T, Busse CE, Wardemann H. Cloning and expression of murine Ig genes from single B cells. J Immunol Methods. 2009;350(1–2):183–193.
  • Kurosawa N, Yoshioka M, Fujimoto R, et al. Rapid production of antigen-specific monoclonal antibodies from a variety of animals. BMC Biol. 2012;10:80–93.
  • Turner KB, Naciri J, Liu JL, et al. Next-generation sequencing of a single domain antibody repertoire reveals quality of phage display selected candidates. PLoS One. 2016;11(2):e0149393.
  • Gray GL, Smith DH, Baldridge JS, et al. Cloning, nucleotide sequence, and expression in Escherichia coli of the exotoxin A structural gene of Pseudomonas aeruginosa. Proc Natl Acad Sci USA. 1984;81(9):2645–2649.
  • Cook DAN, Samarasekara CL, Wagstaff SC, et al. Analysis of camelid IgG for antivenom development: immunoreactivity and preclinical neutralisation of venom-induced pathology by IgG subclasses, and the effect of heat treatment. Toxicon. 2010;56(4):596–603.
  • Picelli S, Faridani OR, Bjorklund AK, et al. Full-length RNA-seq from single cells using Smart-seq2. Nat Protoc. 2014;9(1):171–181.
  • Wang P, Li G, Yan J, et al. Bactrian camel nanobody-based immunoassay for specific and sensitive detection of Cry1Fa toxin. Toxicon. 2014;92:186–192.
  • Ahmadi S, Pucca MB, Jurgensen JA, et al. An in vitro methodology for discovering broadly-neutralizing monoclonal antibodies. Sci Rep. 2020;10(1):10765.
  • Hanyu Y, Kato M. Screening antibody libraries with colony assay using scFv-alkaline phosphatase fusion proteins. Molecules. 2020;25(12):2905.
  • Pardon E, Laeremans T, Triest S, et al. A general protocol for the generation of nanobodies for structural biology. Nat Protoc. 2014;9(3):674–693.
  • Zanjani LS, Shapouri R, Dezfulian M, et al. Exotoxin A-PLGA nanoconjugate vaccine against Pseudomonas aeruginosa infection: protectivity in murine model. World J Microbiol Biotechnol. 2019;35(6):94.
  • Zou G, de Leeuw E. Neutralization of Pseudomonas aeruginosa Exotoxin A by human neutrophil peptide 1. Biochem Biophys Res Commun. 2018;501(2):454–457.
  • Javanmardi F, Emami A, Pirbonyeh N, et al. A systematic review and meta-analysis on exo-toxins prevalence in hospital acquired Pseudomonas aeruginosa isolates. Infect Genet Evol. 2019;75:104037.
  • Balasubramanian V, Sellegounder D, Suman K, et al. Proteome analysis reveals translational inhibition of Caenorhabditis elegans enhances susceptibility to Pseudomonas aeruginosa PAO1 pathogenesis. J Proteomics. 2016;145:141–152.
  • Muller F, Cunningham T, Beers R, et al. Domain II of Pseudomonas exotoxin is critical for efficacy of bolus doses in a xenograft model of acute lymphoblastic leukemia. Toxins (Basel). 2018;10(5):210.
  • Allured VS, Collier RJ, Carroll SF, et al. Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-Angstrom resolution. Proc Natl Acad Sci USA. 1986;83(5):1320–1324.
  • Santajit S, Seesuay W, Mahasongkram K, et al. Human single-chain antibodies that neutralize Pseudomonas aeruginosa-Exotoxin A-mediated cellular apoptosis. Sci Rep. 2019;9(1):14928.