Bioinformatics analysis and prokaryotic expression of a cystatin analogue from Spirometra erinaceieuropaei

References

• Badri M, Olfatifar M, KarimiPourSaryazdi A, et al. The global prevalence of Spirometra parasites in snakes, frogs, dogs, and cats: a systematic review and meta-analysis. Vet Med Sci. 2022;8(6):1–10. doi: 10.1002/vms3.932.
   Web of Science ®Google Scholar
• Liu W, Gong T, Chen S, et al. Epidemiology, diagnosis, and prevention of sparganosis in Asia. Animals. 2022;12(12):1578. doi: 10.3390/ani12121578.
   Web of Science ®Google Scholar
• Liu LN, Wang ZQ, Zhang X, et al. Characterization of Spirometra erinaceieuropaei plerocercoid cysteine protease and potential application for serodiagnosis of sparganosis. PLoS Negl Trop Dis. 2015;9(6):e0003807. doi: 10.1371/journal.pntd.0003807.
   PubMed Web of Science ®Google Scholar
• Liu SN, Su XY, Chen WQ, et al. Transcriptome profiling of plerocercoid and adult developmental stages of the neglected medical tapeworm Spirometra erinaceieuropaei. Acta Trop. 2022;232:106483. doi: 10.1016/j.actatropica.2022.106483.
   PubMed Web of Science ®Google Scholar
• Klotz C, Ziegler T, Daniłowicz-Luebert E, et al. Cystatins of parasitic organisms. Cyst Prot Pathog Org 2011;712:208–221.
   Google Scholar
• Joshi P, Mishra PKK. Functional diversity of the excretory/secretory proteins of nematode parasites. Acta Parasitol. 2022;67(2):619–627. doi: 10.1007/s11686-022-00523-7.
   PubMed Web of Science ®Google Scholar
• Shi W, Xu N, Wang X, et al. Helminth therapy for immune-mediated inflammatory diseases: current and future perspectives. J Inflamm Res. 2022;15:475–491. doi: 10.2147/JIR.S348079.
   PubMed Web of Science ®Google Scholar
• Khatri V, Chauhan N, Kalyanasundaram R. Parasite cystatin: immunomodulatory molecule with therapeutic activity against immune mediated disorders. Pathogens. 2020;9(6):431. doi: 10.3390/pathogens9060431.
   PubMed Web of Science ®Google Scholar
• Chantree P, Tarasuk M, Prathaphan P, et al. Type I cystatin derived from Fasciola gigantica suppresses macrophage-mediated inflammatory responses. Pathogens. 2023;12(3):395. doi: 10.3390/pathogens12030395.
   PubMed Web of Science ®Google Scholar
• Khatri V, Amdare N, Tarnekar A, et al. Brugia malayi cystatin therapeutically ameliorates dextran sulfate sodium-induced colitis in mice. J Dig Dis. 2015;16(10):585–594. doi: 10.1111/1751-2980.12290.
   PubMed Web of Science ®Google Scholar
• Gao S, Li H, Xie H, et al. Therapeutic efficacy of Schistosoma japonicum cystatin on sepsis-induced cardiomyopathy in a mouse model. Parasit Vect. 2020;13(1):260. doi: 10.1186/s13071-020-04104-3.
   PubMed Web of Science ®Google Scholar
• Caraballo L, Zakzuk J, Acevedo N. Helminth-derived cystatins: the immunomodulatory properties of an Ascaris lumbricoides cystatin. Parasitology. 2021;148(14):1–13. doi: 10.1017/S0031182021000214.
   Web of Science ®Google Scholar
• Britton C, Laing R, McNeilly TN, et al. New technologies to study helminth development and host–parasite interactions. Int J Parasitol. 2023;53(8):393–403. doi: 10.1016/j.ijpara.2022.11.012.
   PubMed Web of Science ®Google Scholar
• LV Gang, Y-J, Lu, Z-G, Fan, et al. Construction and identification of a full-length cDNA library from Spirometra erinaceieuropaei. Chinese J Parasitol. 2010;28:393–394.
   PubMedGoogle Scholar
• Huang L, Mai L, Zhong K, et al. Bioinformatics analysis, cloning and expression of Spirometra erinaceieuropaei fatty acid-binding protein. PJZ. 2022;54(3):1027–1035. doi: 10.17582/journal.pjz/20210303090323.
   Web of Science ®Google Scholar
• Simpson RJ. SDS-PAGE of proteins. Cold Spring Harb Protoc. 2006;2006(1):pdb.prot4313. doi: 10.1101/pdb.prot4313.
   Google Scholar
• Kim B. Molecular profiling. Methods in molecular biology. New York (NY): Humana Press; 2017. Chapter 24, Western blot techniques; p. 133–139.
   Google Scholar
• Tang B, Liu M, Wang L, et al. Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage. Parasit Vect. 2015;8(1):78. doi: 10.1186/s13071-015-0689-5.
   PubMedGoogle Scholar
• Das NC, Sen Gupta PS, Biswal S, et al. In-silico evidences on filarial cystatin as a putative ligand of human TLR4. J Biomol Struct Dyn. 2022;40(19):8808–8824. doi: 10.1080/07391102.2021.1918252.
   PubMed Web of Science ®Google Scholar
• Buša M, Matoušková Z, Bartošová-Sojková P, et al. An evolutionary molecular adaptation of an unusual stefin from the liver fluke Fasciola hepatica redefines the cystatin superfamily. J Biol Chem. 2023;299(3):102970. doi: 10.1016/j.jbc.2023.102970.
   PubMed Web of Science ®Google Scholar
• Turk V, Turk D, Dolenc I, et al. Characteristics, structure, and biological role of stefins (type-1 cystatins) of human, mammal, and parasite origin. ACSI. 2019;66(1):5–17. doi: 10.17344/acsi.2018.4639.
   Google Scholar
• Yang X, Liu J, Yue Y, et al. Cloning, expression and characterisation of a type II cystatin from Schistosoma japonicum, which could regulate macrophage activation. Parasitol Res. 2014;113(11):3985–3992. doi: 10.1007/s00436-014-4064-9.
   PubMed Web of Science ®Google Scholar
• Jang SW, Cho MK, Park MK, et al. Parasitic helminth cystatin inhibits DSS-induced intestinal inflammation via IL-10+ F4/80+ macrophage recruitment. Kor J Parasitol. 2011;49(3):245–254. doi: 10.3347/kjp.2011.49.3.245.
   PubMed Web of Science ®Google Scholar
• Kang J-M, Lee K-H, Sohn W-M, et al. Identification and functional characterization of CsStefin-1, a cysteine protease inhibitor of Clonorchis sinensis. Mol Biochem Parasitol. 2011;177(2):126–134. doi: 10.1016/j.molbiopara.2011.02.010.
   PubMed Web of Science ®Google Scholar
• Ilgová J, Jedličková L, Dvořáková H, et al. A novel type I cystatin of parasite origin with atypical legumain-binding domain. Sci Rep. 2017;7(1):17526. doi: 10.1038/s41598-017-17598-2.
   PubMedGoogle Scholar
• Zhou P, Zhou Z, Huayu M, et al. A multi-epitope vaccine GILE against Echinococcus multilocularis infection in mice. Front Immunol. 2022;13:1091004. doi: 10.3389/fimmu.2022.1091004.
   PubMed Web of Science ®Google Scholar
• Lustigman S, James ER, Tawe W, et al. Towards a recombinant antigen vaccine against Onchocerca volvulus. Trends Parasitol. 2002;18(3):135–141. doi: 10.1016/s1471-4922(01)02211-5.
   PubMed Web of Science ®Google Scholar
• Babayan SA, Luo H, Gray N, et al. Deletion of parasite immune modulatory sequences combined with immune activating signals enhances vaccine mediated protection against filarial nematodes. PLoS Negl Trop Dis. 2012;6(12):e1968. doi: 10.1371/journal.pntd.0001968.
   PubMed Web of Science ®Google Scholar
• Arumugam S, Zhan B, Abraham D, et al. Vaccination with recombinant Brugia malayi cystatin proteins alters worm migration, homing and final niche selection following a subcutaneous challenge of mongolian gerbils (Meriones unguiculatus) with B. malayi infective larvae. Parasit Vect. 2014;7(1):43. doi: 10.1186/1756-3305-7-43.
   PubMedGoogle Scholar
• Schnoeller C, Rausch S, Pillai S, et al. A helminth immunomodulator reduces allergic and inflammatory responses by induction of IL-10-producing macrophages. J Immunol. 2008;180(6):4265–4272. doi: 10.4049/jimmunol.180.6.4265.
   PubMed Web of Science ®Google Scholar
• Xu JY, Liu MY, Yu PC, et al. Effect of recombinant Trichinella spiralis cysteine proteinase inhibitor on TNBS-induced experimental inflammatory bowel disease in mice. Int Immunopharmacol. 2019;66(66):28–40. doi: 10.1016/j.intimp.2018.10.043.
   PubMedGoogle Scholar
• Heiber A, Spielmann T. Preparation of parasite protein extracts and Western blot analysis. Bio-protocol. 2014;4(11):e1136–e1136. doi: 10.21769/BioProtoc.1136.
   Google Scholar
• Chung Y-B, Yang H-J. Partial purification and characterization of a cysteine protease inhibitor from the plerocercoid of Spirometra erinacei. Kor J Parasitol. 2008;46(3):183–186. doi: 10.3347/kjp.2008.46.3.183.
   PubMed Web of Science ®Google Scholar
• Karakavuk T, Gül C, Karakavuk M, et al. Biotechnological based recombinant protein vaccines developed against toxoplasmosis. Turkiye Parazitol Derg. 2022;46(4):342–357. doi: 10.4274/tpd.galenos.2022.41636.
   PubMedGoogle Scholar
• Striepen B. Switching parasite proteins on and off. Nat Methods. 2007;4(12):999–1000. doi: 10.1038/nmeth1207-999.
   PubMed Web of Science ®Google Scholar