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Diagnostics

Molecular diagnostic platforms for detecting Aspergillus

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Pages S223-S232 | Received 10 Feb 2008, Published online: 06 Jun 2008

References

  • Maschmeyer G, Haas A. The epidemiology and treatment of infections in cancer patients. Int J Antimicrob Agents 2008; 31: 193–197
  • Marr KA, Carter RA, Crippa F, Wald A, Corey L. Epidemiology and outcome of mould infections in hematopoietic stem cell transplant recipients. Clin Infect Dis 2002; 34: 909–917
  • Bhatti Z, Shaukat A, Almyroudis NG, Segal BH. Review of epidemiology, diagnosis, and treatment of invasive mould infections in allogeneic hematopoietic stem cell transplant recipients. Mycopathologia 2006; 162: 1–15
  • Marr KA, Carter RA, Boeckh M, Martin P, Corey L. Invasive aspergillosis in allogeneic stem cell transplant recipients: changes in epidemiology and risk factors. Blood 2002; 100: 4358–4366
  • Maschmeyer G, Haas A, Cornely OA. Invasive aspergillosis: epidemiology, diagnosis and management in immunocompromised patients. Drugs 2007; 67: 1567–1601
  • Pagano L, Caira M, Nosari A, et al. Fungal infections in recipients of hematopoietic stem cell transplants: results of the SEIFEM B- study – Sorveglianza Epidemiologica Infezioni Fungine Nelle Emopatie Maligne. Clin Infect Dis; 45 2004; 2007: 1161–1170
  • Iversen M, Burton CM, Vand S, et al. Aspergillus infection in lung transplant patients: incidence and prognosis. Eur J Clin Microbiol Infect Dis 2007; 26: 879–886
  • Denning DW. Early diagnosis of invasive aspergillosis. Lancet 2000; 355: 423–424
  • Kibbler C. Defining invasive fungal infections in neutropenic or stem cell transplant patients. J Antimicrob Chemother 2005; 56(Suppl. 1)12–16
  • Ascioglu S, Rex JH, de Pauw B, et al. Defining opportunistic invasive fungal infections in immunocompromised patients with cancer and hematopoietic stem cell transplants: an international consensus. Clin Infect Dis 2002; 34: 7–14
  • Walsh TJ, Anaissie EJ, Denning DW, et al. Treatment of aspergillosis: clinical practice guidelines of the Infectious Diseases Society of America. Clin Infect Dis 2008; 46: 327–360
  • Verweij PE, Mellado E, Melchers WJ. Multiple-triazole-resistant aspergillosis. N Engl J Med 2007; 356: 1481–1483
  • Pfaller MA, Diekema DJ. Epidemiology of invasive candidiasis: a persistent public health problem. Clin Microbiol Rev 2007; 20: 133–163
  • Morrell M, Fraser VJ, Kollef MH. Delaying the empiric treatment of Candida albicans bloodstream infection until positive blood culture results are obtained: a potential risk factor for hospital mortality. Antimicrob Agents Chemother 2005; 49: 3640–3645
  • Ali R, Ozkalemkas F, Ozcelik T, et al. Invasive pulmonary aspergillosis: role of early diagnosis and surgical treatment in patients with acute leukemia. Ann Clin Microbiol Antimicrob 2006; 5: 17
  • Kim MJ, Lee KS, Kim J, et al. Crescent sign in invasive pulmonary aspergillosis: frequency and related CT and clinical factors. J Comput Assist Tomogr 2001; 25: 305–310
  • Greene RE, Schlamm HT, Oestmann JW, et al. Imaging findings in acute invasive pulmonary aspergillosis: clinical significance of the halo sign. Clin Infect Dis 2007; 44: 373–379
  • Verdaguer V, Walsh TJ, Hope W, Cortez KJ. Galactomannan antigen detection in the diagnosis of invasive aspergillosis. Expert Rev Mol Diagn 2007; 7: 21–32
  • Reiss E, Obayashi T, Orle K, Yoshida M, Zancope-Oliveira RM. Non-culture based diagnostic tests for mycotic infections. Med Mycol 2000; 38(Suppl. 1)147–159
  • Sarfati J, Boucias DG, Latge JP. Antigens of Aspergillus fumigatus produced in vivo. J Med Vet Mycol 1995; 33: 9–14
  • Dalle F, Lopez J, Caillot D, et al. False-positive results caused by cotton swabs in commercial Aspergillus antigen latex agglutination test. Eur J Clin Microbiol Infect Dis 2002; 21: 130–132
  • Maertens J, Theunissen K, Verhoef G, Van Eldere J. False-positive Aspergillus galactomannan antigen test results. Clin Infect Dis 2004; 39: 289–290
  • Mattei D, Rapezzi D, Mordini N, et al. False-positive Aspergillus galactomannan enzyme-linked immunosorbent assay results in vivo during amoxicillin-clavulanic acid treatment. J Clin Microbiol 2004; 42: 5362–5363
  • Viscoli C, Machetti M, Cappellano P, et al. False-positive galactomannan platelia Aspergillus test results for patients receiving piperacillin-tazobactam. Clin Infect Dis 2004; 38: 913–916
  • Donnelly JP. Polymerase chain reaction for diagnosing invasive aspergillosis: getting closer but still a ways to go. Clin Infect Dis 2006; 42: 487–489
  • Ferns RB. Evaluation of the role of real-time PCR in the diagnosis of invasive aspergillosis. Leuk Lymphoma 2006; 47: 15–20
  • El-Mahallawy HA, Shaker HH, Ali Helmy H, et al. Evaluation of pan-fungal PCR assay and Aspergillus antigen detection in the diagnosis of invasive fungal infections in high risk paediatric cancer patients. Med Mycol 2006; 44: 733–739
  • Scotter JM, Campbell P, Anderson TP, et al. Comparison of PCR-ELISA and galactomannan detection for the diagnosis of invasive aspergillosis. Pathology 2005; 37: 246–253
  • Scotter JM, Chambers ST. Comparison of galactomannan detection, PCR-enzyme-linked immunosorbent assay, and real-time PCR for diagnosis of invasive aspergillosis in a neutropenic rat model and effect of caspofungin acetate. Clin Diagn Lab Immunol 2005; 12: 1322–1327
  • Kami M, Fukui T, Ogawa S, et al. Use of real-time PCR on blood samples for diagnosis of invasive aspergillosis. Clin Infect Dis 2001; 33: 1504–1512
  • Millon L, Piarroux R, Deconinck E, et al. Use of real-time PCR to process the first galactomannan-positive serum sample in diagnosing invasive aspergillosis. J Clin Microbiol 2005; 43: 5097–5101
  • Lass-Florl C, Gunsilius E, Gastl G, et al. Clinical evaluation of Aspergillus-PCR for detection of invasive aspergillosis in immunosuppressed patients. Mycoses 2005; 48(Suppl. 1)12–17
  • Halliday C, Wu QX, James G, Sorrell T. Development of a nested qualitative real-time PCR assay to detect Aspergillus species DNA in clinical specimens. J Clin Microbiol 2005; 43: 5366–5368
  • Chryssanthou E, Klingspor L, Tollemar J, et al. PCR and other non-culture methods for diagnosis of invasive Candida albicans infections in allogeneic bone marrow and solid organ transplant recipients. Mycoses 1999; 42: 239–247
  • Buchheidt D, Hummel M. Aspergillus polymerase chain reaction (PCR) diagnosis. Med Mycol 2005; 43(Suppl. 1)S139–145
  • Bretagne S, Costa JM. Towards a molecular diagnosis of invasive aspergillosis and disseminated candidosis. FEMS Immunol Med Microbiol 2005; 45: 361–368
  • Musher B, Fredricks D, Leisenring W, et al. Aspergillus galactomannan enzyme immunoassay and quantitative PCR for diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. J Clin Microbiol 2004; 42: 5517–5522
  • Hummel M, Baust C, Kretschmar M, et al. Detection of Aspergillus DNA by a nested PCR assay is superior to blood culture in an experimental murine model of invasive aspergillosis. J Med Microbiol 2004; 53(Pt 8)803–806
  • Buchheidt D, Hummel M, Schleiermacher D, et al. Prospective clinical evaluation of a LightCycler-mediated polymerase chain reaction assay, a nested-PCR assay and a galactomannan enzyme-linked immunosorbent assay for detection of invasive aspergillosis in neutropenic cancer patients and haematological stem cell transplant recipients. Br J Haematol 2004; 125: 196–202
  • Lass-Florl C, Aigner J, Gunsilius E, et al. Screening for Aspergillus spp. using polymerase chain reaction of whole blood samples from patients with haematological malignancies. Br J Haematol 2001; 113: 180–184
  • Buchheidt D, Baust C, Skladny H, et al. Detection of Aspergillus species in blood and bronchoalveolar lavage samples from immunocompromised patients by means of 2-step polymerase chain reaction: clinical results. Clin Infect Dis 2001; 33: 428–435
  • Skladny H, Buchheidt D, Baust C, Krieg-Schneider F, Seifarth W, Leib-Mosch C, et al. Specific detection of Aspergillus species in blood and bronchoalveolar lavage samples of immunocompromised patients by two-step PCR. J Clin Microbiol 1999; 37: 3865–3871
  • Yamakami Y, Hashimoto A, Yamagata E, et al. Evaluation of PCR for detection of DNA specific for Aspergillus species in sera of patients with various forms of pulmonary aspergillosis. J Clin Microbiol 1998; 36: 3619–3623
  • Klingspor L, Jalal S. Molecular detection and identification of Candida albicans and Aspergillus spp. from clinical samples using real-time PCR. Clin Microbiol Infect 2006; 12: 745–753
  • Perlin DS, Park S. Rapid identification of fungal pathogens: molecular approaches for a new millennium. Rev Med Microbiol 2001; 12((suppl.))S13–20
  • Perlin DS. Resistance to echinocandin-class antifungal drugs. Drug Resist Updat 2007; 10: 121–130
  • Varga J, Toth B, Rigo K, et al. Phylogenetic analysis of Aspergillus section Circumdati based on sequences of the internal transcribed spacer regions and the 5.8 S rRNA gene. Fungal Genet Biol 2000; 30: 71–80
  • Nierman WC, May G, Kim HS, et al. What the Aspergillus genomes have told us. Med Mycol 2005; 43(Suppl. 1)S3–5
  • Ronning CM, Fedorova ND, Bowyer P, Coulson R, Goldman G, Kim HS, et al. Genomics of Aspergillus fumigatus. Rev Iberoam Micol 2005; 22: 223–228
  • Hinrikson HP, Hurst SF, Lott TJ, Warnock DW, Morrison CJ. Assessment of ribosomal large-subunit D1-D2, internal transcribed spacer 1, and internal transcribed spacer 2 regions as targets for molecular identification of medically important Aspergillus species. J Clin Microbiol 2005; 43: 2092–2103
  • Henry T, Iwen PC, Hinrichs SH. Identification of Aspergillus species using internal transcribed spacer regions 1 and 2. J Clin Microbiol 2000; 38: 1510–1515
  • Perlin DS. Multiplex detection of mutations. Methods in Molecular Biology, AMaO Seitz. The Humana Press Inc, Totowa, NJ 2007
  • Compton J. Nucleic acid sequence-based amplification. Nature 1991; 350: 91–92
  • Malek L, Sooknanan R, Compton J. Nucleic acid sequence-based amplification (NASBA). Methods Mol Biol 1994; 28: 253–260
  • Romano JW, van Gemen B, Kievits T. NASBA: a novel, isothermal detection technology for qualitative and quantitative HIV-1 RNA measurements. Clin Lab Med 1996; 16: 89–103
  • Turner DH. Thermodynamics of base pairing. Curr Opin Struct Biol 1996; 6: 299–304
  • Perlin DS. Application of real-time PCR to the diagnosis of invasive fungal infections. Real-time PCR current technology and applications, J. Logan, K. Edwards, N. Sanders. Horizon Scientific Press, NorwichUK 2008
  • Balashov SV, Gardiner R, Park S, Perlin DS. Rapid, high-throughput, multiplex, real-time PCR for identification of mutations in the cyp51A gene of Aspergillus fumigatus that confer resistance to itraconazole. J Clin Microbiol 2005; 43: 214–222
  • Hebart H, Loffler J, Reitze H, et al. Prospective screening by a panfungal polymerase chain reaction assay in patients at risk for fungal infections: implications for the management of febrile neutropenia. Br J Haematol 2000; 111: 635–640
  • Van Burik JA, Myerson D, Schreckhise RW, Bowden RA. Panfungal PCR assay for detection of fungal infection in human blood specimens. J Clin Microbiol 1998; 36: 1169–1175
  • Hendolin PH, Paulin L, Koukila-Kahkola P, et al. Panfungal PCR and multiplex liquid hybridization for detection of fungi in tissue specimens [In Process Citation]. J Clin Microbiol 2000; 38: 4186–4192
  • Buchheidt D, Baust C, Skladny H, et al. Clinical evaluation of a polymerase chain reaction assay to detect Aspergillus species in bronchoalveolar lavage samples of neutropenic patients. Br J Haematol 2002; 116: 803–811
  • Costa C, Costa JM, Desterke C, et al. Real-time PCR coupled with automated DNA extraction and detection of galactomannan antigen in serum by enzyme-linked immunosorbent assay for diagnosis of invasive aspergillosis. J Clin Microbiol 2002; 40: 2224–2227
  • Loeffler J, Kloepfer K, Hebart H, et al. Polymerase chain reaction detection of Aspergillus DNA in experimental models of invasive aspergillosis. J Infect Dis 2002; 185: 1203–1206
  • Loeffler J, Henke N, Hebart H, Schmidt D, Hagmeyer L, Schumacher U, et al. Quantification of fungal DNA by using fluorescence resonance energy transfer and the light cycler system. J Clin Microbiol 2000; 38: 586–590
  • Spiess B, Buchheidt D, Baust C, et al. Development of a LightCycler PCR assay for detection and quantification of Aspergillus fumigatus DNA in clinical samples from neutropenic patients. J Clin Microbiol 2003; 41: 1811–1818
  • Loeffler J, Hebart H, Cox P, et al. Nucleic acid sequence-based amplification of Aspergillus RNA in blood samples. J Clin Microbiol 2001; 39: 1626–1629
  • Gomez-Lopez A, Garcia-Effron G, Mellado E, et al. In vitro activities of three licensed antifungal agents against Spanish clinical isolates of Aspergillus spp. Antimicrob Agents Chemother 2003; 47: 3085–3088
  • Mosquera J, Denning DW. Azole cross-resistance in Aspergillus fumigatus. Antimicrob Agents Chemother 2002; 46: 556–557
  • Maertens JA, Klont R, Masson C, et al. Optimization of the cut-off value for the Aspergillus double-sandwich enzyme immunoassay. Clin Infect Dis 2007; 44: 1329–1336
  • Hope WW, Warn PA, Sharp A, et al. Derivation of an in vivo drug exposure breakpoint for flucytosine against Candida albicans and impact of the MIC, growth rate, and resistance genotype on the antifungal effect. Antimicrob Agents Chemother 2006; 50: 3680–3688
  • Mellado E, Diaz-Guerra TM, Cuenca-Estrella M, et al. Identification of two different 14-alpha sterol demethylase-related genes (cyp51A and cyp51B) in Aspergillus fumigatus and other Aspergillus species. J Clin Microbiol 2001; 39: 2431–2438
  • Garcia-Effron G, Dilger A, Alcazar-Fuoli L, et al. Rapid detection of triazole antifungal resistance in Aspergillus fumigatus. J Clin Microbiol 2008; 46: 1200–1206
  • Balashov SV, Park S, Perlin DS. Assessing resistance to the echinocandin antifungal drug caspofungin in Candida albicans by profiling mutations in FKS1. Antimicrob Agents Chemother 2006; 50: 2058–2063
  • Gardiner RE, Souteropoulos P, Park S, Perlin DS. Characterization of Aspergillus fumigatus mutants with reduced susceptibility to caspofungin. Med Mycol 2005; 43(Suppl. 1)S299–305

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