Comparative transcriptome and iTRAQ-based proteome analysis in mature leaves of Brassica carinata provides insights into the purple leaf color diversity

References

• Abebe, D., Dawit, T., Getahun, M., & Debritu, B. (1992). Ethiopia’s genetic resources of oilseeds. Proc. 1 National Oilseeds Workshop (pp. 12–17). Addis Ababa, Ethiopia: Institute of Agricultural Research.
   Google Scholar
• Al Sane, K.O., & Hesham, A.E.-L. (2015). Biochemical and genetic evidences of anthocyanin biosynthesis and accumulation in a selected tomato mutant. Rendiconti Lincei, 26, 293–306. doi:10.1007/s12210-015-0446-x
   Google Scholar
• Alemayehu, N., & Becker, H. (2002). Genotypic diversity and patterns of variation in a germplasm material of Ethiopian mustard (Brassica carinata A. Braun). Genetic Resources and Crop Evolution, 49, 573–582. doi:10.1023/A:1021204412404
   Web of Science ®Google Scholar
• Asare, E.K., Båga, M., Rossnagel, B.G., & Chibbar, R.N. (2012). Polymorphism in the Barley granule bound starch synthase 1 (Gbss1) gene associated with grain starch variant amylose concentration. Journal of Agricultural and Food Chemistry, 60, 10082–10092. doi:10.1021/jf302291t
   PubMed Web of Science ®Google Scholar
• Bowles, D. (2002). A multigene family of glycosyltransferases in a model plant, Arabidopsis thaliana. Biochemical Society Transactions, 30, 301–306. doi:10.1042/bst0300301
   PubMed Web of Science ®Google Scholar
• Chen, J., Liu, S.S., Kohler, A., Yan, B., Luo, H.M., Chen, X.M., & Guo, S.X. (2017). iTRAQ and RNA-seq analyses provide new insights into regulation mechanism of symbiotic germination of dendrobium officinale seeds (Orchidaceae). Journal of Proteome Research, 16, 2174–2187.
   PubMed Web of Science ®Google Scholar
• Chen, T., Zhang, L., Shang, H., Liu, S., Peng, J., Gong, W., … Yuan, Y. (2016). iTRAQ-based quantitative proteomic analysis of cotton roots and leaves reveals pathways associated with salt stress. PLoS One, 11, e0148487–e0148487. doi:10.1371/journal.pone.0148487
   PubMed Web of Science ®Google Scholar
• Cheng, G.W., & Breen, P.J. (1991). Activity of phenylalanine ammonia-lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. Journal of the American Society for Horticultural Science, 116(5), 865–869.
   Web of Science ®Google Scholar
• Chiou, T.-J., & Bush, D.R. (1998). Sucrose is a signal molecule in assimilate partitioning. Proceedings of the National Academy of Sciences, 95, 4784–4788. doi:10.1073/pnas.95.8.4784
   PubMed Web of Science ®Google Scholar
• Chu, P., Yan, G.X., Yang, Q., Zhai, L.N., Zhang, C., Zhang, F.Q., & Guan, R.Z. (2015). iTRAQ-based quantitative proteomics analysis of Brassica napus leaves reveals pathways associated with chlorophyll deficiency. Journal of Proteomics, 113, 244–259. doi:10.1016/j.jprot.2014.10.005
   PubMed Web of Science ®Google Scholar
• Conesa, A., Götz, S., García-Gómez, J.M., Terol, J., Talón, M., & Robles, M. (2005). Blast2GO: A universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics, 21, 3674–3676. doi:10.1093/bioinformatics/bti610
   PubMed Web of Science ®Google Scholar
• D’auria, J.C., Reichelt, M., Luck, K., Svatoš, A., & Gershenzon, J. (2007). Identification and characterization of the BAHD acyltransferase malonyl CoA: Anthocyanidin 5-O-glucoside-6″-O-malonyltransferase (At5MAT) in Arabidopsis thaliana. FEBS Letters, 581, 872–878. doi:10.1016/j.febslet.2007.01.060
   PubMed Web of Science ®Google Scholar
• Dong, T., Han, R., Yu, J., Zhu, M., Zhang, Y., Gong, Y., & Li, Z. (2019). Anthocyanins accumulation and molecular analysis of correlated genes by metabolome and transcriptome in green and purple asparaguses (Asparagus officinalis, L.). Food Chemistry, 271, 18–28. doi:10.1016/j.foodchem.2018.07.120
   PubMed Web of Science ®Google Scholar
• Ferarsa, S., Zhang, W., Moulai-Mostefa, N., Ding, L., Jaffrin, M.Y., & Grimi, N. (2018). Recovery of anthocyanins and other phenolic compounds from purple eggplant peels and pulps using ultrasonic-assisted extraction. Food and Bioproducts Processing, 109, 19–28. doi:10.1016/j.fbp.2018.02.006
   Web of Science ®Google Scholar
• Fofana, B., D.J. McNally, C. Labbe, R. Boulanger, N. Benhamou, A. Seguin, and R.R. Belanger. (2002). Milsana-induced resistance in powdery mildew-infected cucumber plants correlates with the induction of chalcone synthase and chalcone isomerase. Molecular Plant Pathology. 61:121-132. doi: 10.1006/pmpp.2002.0420
   Google Scholar
• Fu, D., Jiang, L., Mason, A.S., Xiao, M., Zhu, L., Li, L., … Huang, C.-H. (2016). Research progress and strategies for multifunctional rapeseed: A case study of China. Journal of Integrative Agriculture, 15, 1673–1684. doi:10.1016/S2095-3119(16)61384-9
   Web of Science ®Google Scholar
• Gamel, T.H., Wright, A.J., Tucker, A.J., Pickard, M., Rabalski, I., Podgorski, M., … Abdel-Aal, E.-S.M. (2019). Absorption and metabolites of anthocyanins and phenolic acids after consumption of purple wheat crackers and bars by healthy adults. Journal of Cereal Science, 86, 60–68. doi:10.1016/j.jcs.2018.11.017
   Web of Science ®Google Scholar
• Ge, X., Zhang, C., Wang, Q., Yang, Z., Wang, Y., Zhang, X., … Li, F. (2015). iTRAQ protein profile differential analysis between somatic globular and cotyledonary embryos reveals stress, hormone, and respiration involved in increasing plantlet regeneration of Gossypium hirsutum L. Journal of Proteome Research, 14, 268–278. doi:10.1021/pr500688g
   PubMed Web of Science ®Google Scholar
• Getinet, A., Rakow, G., & Downey, R.K. (1996). Agronomic performance and seed quality of ethiopian mustard in saskatchewan. Canadian Journal of Plant Science, 76, 387–392. doi:10.4141/cjps96-069
   Web of Science ®Google Scholar
• Guo, S., Zou, J., Li, R., Long, Y., Chen, S., & Meng, J. (2012). A genetic linkage map of Brassica carinata constructed with a doubled haploid population. Theoretical and Applied Genetics, 125, 1113–1124. doi:10.1007/s00122-012-1898-3
   PubMed Web of Science ®Google Scholar
• Guo, Y., Chang, X., Zhu, C., Zhang, S., Li, X., Fu, H., … Lai, Z. (2019). De novo transcriptome combined with spectrophotometry and gas chromatography-mass spectrometer (GC-MS) reveals differentially expressed genes during accumulation of secondary metabolites in purple-leaf tea (Camellia sinensis cv Hongyafoshou). The Journal of Horticultural Science and Biotechnology, 94, 349–367. doi:10.1080/14620316.2018.1521708
   Web of Science ®Google Scholar
• Hu, J., & Quiros, C.F. (1996). Application of molecular markers and cytogenetic stocks to brassica genetics, breeding and evolution. Acta horticulturae, 79–86. doi:10.17660/ActaHortic.1996.407.8
   Google Scholar
• Jiang, Y., Tian, E., Li, R., Chen, L., & Meng, J. (2007). Genetic diversity of Brassica carinata with emphasis on the interspecific crossability with B. rapa. Plant Breeding, 126, 487–491. doi:10.1111/j.1439-0523.2007.01393.x
   Web of Science ®Google Scholar
• Khattak, A.N., Wang, T., Yu, K., Yang, R., Wan, W., Ye, B., & Tian, E. (2019). Exploring the basis of 2-propenyl and 3-butenyl glucosinolate synthesis by QTL mapping and RNA-sequencing in Brassica juncea. PLoS One, 14, e0220597. doi:10.1371/journal.pone.0220597
   PubMed Web of Science ®Google Scholar
• Khoo, H.E., Azlan, A., Tang, S.T., & Lim, S.M. (2017). Anthocyanidins and anthocyanins: Colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food & Nutrition Research, 61, 1361779. doi:10.1080/16546628.2017.1361779
   PubMed Web of Science ®Google Scholar
• Lai, Y.-S., Li, S., Tang, Q., Li, H.-X., Chen, S.-X., Li, P.-W., … Guo, X. (2016). The dark-purple tea cultivar ‘Ziyan’ accumulates a large amount of delphinidin-related anthocyanins. Journal of Agricultural and Food Chemistry, 64, 2719–2726. doi:10.1021/acs.jafc.5b04036
   PubMed Web of Science ®Google Scholar
• Lao, F., & Giusti, M.M. (2016). Quantification of purple corn (Zea mays L.) anthocyanins using spectrophotometric and HPLC approaches: Method comparison and correlation. Food Analytical Methods, 9, 1367–1380. doi:10.1007/s12161-015-0318-0
   Web of Science ®Google Scholar
• Li, D., Zhang, X., Xu, Y., Li, L., Aghdam, M.S., & Luo, Z. (2019). Effect of exogenous sucrose on anthocyanin synthesis in postharvest strawberry fruit. Food Chemistry, 289, 112–120. doi:10.1016/j.foodchem.2019.03.042
   PubMed Web of Science ®Google Scholar
• Li, H., Zhu, L., Yuan, G., Heng, S., Yi, B., Ma, C., … Wen, J. (2016). Fine mapping and candidate gene analysis of an anthocyanin-rich gene, BnaA.PL1, conferring purple leaves in Brassica napus L. Molecular Genetics and Genomics, 291, 1523–1534. doi:10.1007/s00438-016-1199-7
   PubMed Web of Science ®Google Scholar
• Lukens, L.N., Quijada, P.A., Udall, J., Pires, J.C., Schranz, M.E., & Osborn, T.C. (2004). Genome redundancy and plasticity within ancient and recent Brassica crop species. Biological Journal of the Linnean Society, 82, 665–674. doi:10.1111/j.1095-8312.2004.00352.x
   Web of Science ®Google Scholar
• Luo, X., Cao, D., Li, H., Zhao, D., Xue, H., Niu, J., … Cao, S. (2018). Complementary iTRAQ-based proteomic and RNA sequencing-based transcriptomic analyses reveal a complex network regulating pomegranate (Punica granatum L.) fruit peel colour. Scientific Reports, 8, 12362. doi:10.1038/s41598-018-30088-3
   PubMed Web of Science ®Google Scholar
• Mazza, G., & Miniati, E. (1993). Anthocyanins in fruits, vegetables, and grains. In Anthocyanins in fruits vegetables & grains. CRC press.
   Google Scholar
• Meng, J., Shi, S., Gan, L., Li, Z., & Qu, X. (1998). The production of yellow-seeded Brassica napus (AACC) through crossing interspecific hybrids of B. campestris (AA) and B. carinata (BBCC) with B. napus. Euphytica, 103, 329–333. doi:10.1023/A:1018646223643
   Web of Science ®Google Scholar
• Miyahara, T., Sakiyama, R., Ozeki, Y., & Sasaki, N. (2013). Acyl-glucose-dependent glucosyltransferase catalyzes the final step of anthocyanin formation in Arabidopsis. Journal of plant physiology, 170(6), 619–624. doi:10.1016/j.jplph.2012.12.001
   Google Scholar
• Morreel, K., Goeminne, G., Storme, V., Sterck, L., Ralph, J., Coppieters, W., ... & Boerjan, W. (2006). Genetical metabolomics of flavonoid biosynthesis in Populus: a case study. The Plant Journal, 47(2), 224–237. doi: 10.1111/j.1365-313X.2006.02786.x
   Google Scholar
• Muers, M. (2011). Transcriptome to proteome and back to genome. Nature Reviews. Genetics, 12, 518. doi:10.1038/nrg3037
   PubMed Web of Science ®Google Scholar
• Ou, L.-J., Zhang, Z.-Q., Dai, X.-Z., & Zou, -X.-X. (2013). Photooxidation tolerance characters of a new purple pepper. PLoS One, 8, e63593. doi:10.1371/journal.pone.0063593
   PubMed Web of Science ®Google Scholar
• Pandey, A., & Mann, M. (2000). Proteomics to study genes and genomes. Nature, 405, 837–846. doi:10.1038/35015709
   PubMed Web of Science ®Google Scholar
• Pandey, A., Alok, A., Lakhwani, D., Singh, J., Asif, M. H., & Trivedi, P. K. (2016). Genome-wide expression analysis and metabolite profiling elucidate transcriptional regulation of flavonoid biosynthesis and modulation under abiotic stresses in banana. Scientific reports, 6, 31361. doi:10.1038/srep31361
   Google Scholar
• Pelletier, M. K., & Shirley, B. W. (1996). Analysis of flavanone 3-hydroxylase in Arabidopsis seedlings (Coordinate regulation with chalcone synthase and chalcone isomerase). Plant physiology, 111(1), 339–345. doi:10.1104/pp.111.1.339
   PubMed Web of Science ®Google Scholar
• Pojer, E., Mattivi, F., Johnson, D., & Stockley, C.S. (2013). The case for anthocyanin consumption to promote human health: A review. Comprehensive Reviews in Food Science and Food Safety, 12, 483–508.
   PubMed Web of Science ®Google Scholar
• Prior, R.L., & Xianli, W. (2006). Anthocyanins: Structural characteristics that result in unique metabolic patterns and biological activities. Free Radical Research, 40, 1014–1028. doi:10.1080/10715760600758522
   PubMed Web of Science ®Google Scholar
• Shirley, B. W. (1996). Flavonoid biosynthesis:‘new’functions for an ‘old’pathway. Trends in plant science, 1(11), 377-382. doi:10.1016/S1360-1385(96)80312-8
   Google Scholar
• Song, K., Osborn, T.C., & Williams, P.H. (1990). Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs). Theoretical and Applied Genetics, 79, 497–506. doi:10.1007/BF00226159
   PubMed Web of Science ®Google Scholar
• Sun, D., Huang, S., Cai, S., Cao, J., & Han, P. (2015). Digestion property and synergistic effect on biological activity of purple rice (Oryza sativa L.) anthocyanins subjected to a simulated gastrointestinal digestion in vitro. Food Research International, 78, 114–123. doi:10.1016/j.foodres.2015.10.029
   PubMed Web of Science ®Google Scholar
• Szymanowska, U., Złotek, U., Karaś, M., & Baraniak, B. (2015). Anti-inflammatory and antioxidative activity of anthocyanins from purple basil leaves induced by selected abiotic elicitors. Food Chemistry, 172, 71–77. doi:10.1016/j.foodchem.2014.09.043
   PubMed Web of Science ®Google Scholar
• Taylor, D.C., Falk, K.C., Palmer, C.D., Hammerlindl, J., Babic, V., Mietkiewska, E., … Keller, W.A. (2010). Brassica carinata – A new molecular farming platform for delivering bio-industrial oil feedstocks: Case studies of genetic modifications to improve very long-chain fatty acid and oil content in seeds. Biofuels, Bioproducts and Biorefining, 4, 538–561.
   Web of Science ®Google Scholar
• Tian, E., Jiang, Y., Chen, L., Zou, J., Liu, F., & Meng, J. (2010). Synthesis of a Brassica trigenomic allohexaploid (B. carinata × B. rapa) de novo and its stability in subsequent generations. Theoretical and Applied Genetics, 121, 1431–1440. doi:10.1007/s00122-010-1399-1
   PubMed Web of Science ®Google Scholar
• Tohge, T., Nishiyama, Y., Hirai, M.Y., Yano, M., Nakajima, J.-I., Awazuhara, M., … Saito, K. (2005). Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor. The Plant Journal, 42, 218–235. doi:10.1111/j.1365-313X.2005.02371.x
   PubMed Web of Science ®Google Scholar
• Trapnell, C., Roberts, A., Goff, L., Pertea, G., Kim, D., Kelley, D.R., … Pachter, L. (2012). Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nature Protocols, 7, 562–578. doi:10.1038/nprot.2012.016
   PubMed Web of Science ®Google Scholar
• Trapnell, C., Williams, B.A., Pertea, G., Mortazavi, A., Kwan, G., Van Baren, M.J., … Pachter, L. (2010). Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation. Nature Biotechnology, 28, 511–515.
   PubMed Web of Science ®Google Scholar
• Turturica, M., Oancea, A.-M., Rapeanu, G., & Bahrim, G. (2015). Anthocyanins: Naturally occurring fruit pigments with functional properties. Annals of the University Dunarea De Jos of Galati. Fascicle VI: Food Technology, 39, 9–24.
   Web of Science ®Google Scholar
• U. (1935). Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Journal of Japanese Bontany, 7, 389–452.
   Google Scholar
• Wang, A., Li, R., Ren, L., Gao, X., Zhang, Y., Ma, Z., … Luo, Y. (2018a). A comparative metabolomics study of flavonoids in sweet potato with different flesh colors (Ipomoea batatas (L.) Lam). Food Chemistry, 260, 124–134. doi:10.1016/j.foodchem.2018.03.125
   PubMed Web of Science ®Google Scholar
• Wang, C., Li, H., Li, Y., Meng, Q., Xie, F., Xu, Y., & Wan, Z. (2019). Genetic characterization and fine mapping BrCER4 in involved cuticular wax formation in purple cai-tai (Brassica rapa L. var. purpurea). Molecular Breeding, 39, 12. doi:10.1007/s11032-018-0919-6
   Web of Science ®Google Scholar
• Wang, J., Zhang, H., Zhang, J., Zhang, H., Liu, B., Hou, Y., & Qiu, N. (2018b). Changes of anthocyanin content and photosynthetic function of purple leaf plum leaves during spring maturation. Forestry Science & Technology, 43, 13–17.
   Google Scholar
• Wang, L., Feng, Z., Wang, X., Wang, X., & Zhang, X. (2010). DEGseq: An R package for identifying differentially expressed genes from RNA-seq data. Bioinformatics, 26, 136–138. doi:10.1093/bioinformatics/btp612
   PubMed Web of Science ®Google Scholar
• Wang, S., Pan, D., Lv, X., Song, X., Qiu, Z., Huang, C., … Chen, W. (2016). Proteomic approach reveals that starch degradation contributes to anthocyanin accumulation in tuberous root of purple sweet potato. Journal of Proteomics, 143, 298–305. doi:10.1016/j.jprot.2016.03.010
   PubMed Web of Science ®Google Scholar
• Wang, W., Zhang, D., Yu, S., Liu, J., Wang, D., Zhang, F., … Su, T. (2014). Mapping the BrPur gene for purple leaf color on linkage group A03 of Brassica rapa. Euphytica, 199, 293–302. doi:10.1007/s10681-014-1128-y
   Web of Science ®Google Scholar
• Wang, Z., Cui, Y., Vainstein, A., Chen, S., & Ma, H. (2017). Regulation of Fig (Ficus carica L.) fruit color: Metabolomic and transcriptomic analyses of the flavonoid biosynthetic pathway. Frontiers in Plant Science, 8, 1990. doi:10.3389/fpls.2017.01990
   PubMed Web of Science ®Google Scholar
• Warwick, S.I. (2011). Brassicaceae in agriculture. In R. Schmidt & I. Bancroft (Eds.), Genetics and genomics of the brassicaceae (pp. 33–65). New York: Springer New York.
   Google Scholar
• Warwick, S.I., Gugel, R.K., Mcdonald, T., & Falk, K.C. (2006). Genetic variation of Ethiopian mustard (Brassica carinata A. Braun) Germplasm in Western Canada. Genetic Resources and Crop Evolution, 53, 297–312. doi:10.1007/s10722-004-6108-y
   Web of Science ®Google Scholar
• Wiese, S., Reidegeld, K.A., Meyer, H.E., & Warscheid, B. (2007). Protein labeling by iTRAQ: A new tool for quantitative mass spectrometry in proteome research. PROTEOMICS, 7, 340–350. doi:10.1002/pmic.200600422
   PubMed Web of Science ®Google Scholar
• Wilmouth, R. C., Turnbull, J. J., Welford, Richard W.D.., Clifton, I. J., Prescott, A. G., & Schofield, C. J. (2002). Structure and mechanism of anthocyanidin synthase from Arabidopsis thaliana. Structure, 10(1), 93–103. doi:10.1016/S0969-2126(01)00695-5
   PubMed Web of Science ®Google Scholar
• Winkel-Shirley, B. (2001). Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant physiology, 126(2), 485–493. https://doi.org/10.1104/pp.126.2.485
   Google Scholar
• Xiao, Y., Chen, L., Zou, J., Tian, E., Xia, W., & Meng, J. (2010). Development of a population for substantial new type Brassica napus diversified at both A/C genomes. Theoretical and Applied Genetics, 121, 1141–1150. doi:10.1007/s00122-010-1378-6
   PubMed Web of Science ®Google Scholar
• Yan, C., An, G., Zhu, T., Zhang, W., Zhang, L., Peng, L., … Kuang, H. (2019). Independent activation of the BoMYB2 gene leading to purple traits in Brassica oleracea. Theoretical and Applied Genetics, 132, 895–906. doi:10.1007/s00122-018-3245-9
   PubMed Web of Science ®Google Scholar
• Yang, L.-T., Qi, Y.-P., Lu, Y.-B., Guo, P., Sang, W., Feng, H., … Chen, L.-S. (2013). iTRAQ protein profile analysis of Citrus sinensis roots in response to long-term boron-deficiency. Journal of Proteomics, 93, 179–206. doi:10.1016/j.jprot.2013.04.025
   PubMed Web of Science ®Google Scholar
• Yonekura‐Sakakibara, K., Fukushima, A., Nakabayashi, R., Hanada, K., Matsuda, F., Sugawara, S., ... & Wangwattana, B. (2012). Two glycosyltransferases involved in anthocyanin modification delineated by transcriptome independent component analysis in Arabidopsis thaliana. The Plant Journal, 69(1), 154–167. doi:10.1111/j.1365-313X.2011.04779.x
   PubMed Web of Science ®Google Scholar
• Yu, X. (2000). Analysis and study of anthocyanins in plant leave. Modern Instruments, 4, 37–38.
   Google Scholar
• Zhang, H., Hassan, Y.I., Renaud, J., Liu, R., Yang, C., Sun, Y., & Tsao, R. (2017a). Bioaccessibility, bioavailability, and anti-inflammatory effects of anthocyanins from purple root vegetables using mono- and co-culture cell models. Molecular Nutrition & Food Research, 61, 1600928. doi:10.1002/mnfr.201600928
   Web of Science ®Google Scholar
• Zhang, W., Hu, D., Raman, R., Guo, S., Wei, Z., Shen, X., … Zou, J. (2017b). Investigation of the genetic diversity and quantitative trait loci accounting for important agronomic and seed quality traits in Brassica carinata. Frontiers in Plant Science, 8. doi:10.3389/fpls.2017.00615
   Web of Science ®Google Scholar
• Zhao, Z., Xiao, L., Xu, L., Xing, X., Tang, G., & Du, D. (2017). Fine mapping the BjPl1 gene for purple leaf color in B2 of Brassica juncea L. through comparative mapping and whole-genome re-sequencing. Euphytica, 213, 80. doi:10.1007/s10681-017-1868-6
   Web of Science ®Google Scholar
• Ziegler, H. (1975). Nature of transported substances. In: M.H. Zimmermann & J.A. Milburn (Eds.), Transport in plants I: Phloem transport (pp. 59–100). Berlin, Heidelberg: Springer Berlin Heidelberg.
   Google Scholar
• Zou, J., Hu, D., Mason, A.S., Shen, X., Wang, X., Wang, N., … Meng, J. (2018). Genetic changes in a novel breeding population of Brassica napus synthesized from hundreds of crosses between B. rapa and B. carinata. Plant Biotechnology Journal, 16, 507–519. doi:10.1111/pbi.12791
   PubMed Web of Science ®Google Scholar
• Zou, J., Raman, H., Guo, S., Hu, D., Wei, Z., Luo, Z., … Meng, J. (2014). Constructing a dense genetic linkage map and mapping QTL for the traits of flower development in Brassica carinata. Theoretical and Applied Genetics, 127, 1593–1605. doi:10.1007/s00122-014-2321-z
   PubMed Web of Science ®Google Scholar
• Zou, J., Zhu, J., Huang, S., Tian, E., Xiao, Y., Fu, D., … Meng, J. (2010). Broadening the avenue of intersubgenomic heterosis in oilseed Brassica. Theoretical and Applied Genetics, 120, 283–290. doi:10.1007/s00122-009-1201-4
   PubMed Web of Science ®Google Scholar