The search for better treatment strategies for mansonellosis: an expert perspective

References

• Simonsen PE, Onapa AW, Asio SM. Mansonella perstans filariasis in Africa. Acta Trop. 2011;120 Suppl 1:S109–20. doi: 10.1016/j.actatropica.2010.01.014
   PubMed Web of Science ®Google Scholar
• Lima NF, Veggiani Aybar CA, Dantur Juri MJ, et al. Mansonella ozzardi: a neglected New World filarial nematode. Pathog Glob Health. 2016;110(3):97–107. doi: 10.1080/20477724.2016.1190544
   PubMed Web of Science ®Google Scholar
• Ta-Tang TH, Luz SLB, Crainey JL, et al. An overview of the management of mansonellosis. Res Rep Trop Med. 2021;12:93–105. doi: 10.2147/RRTM.S274684
   PubMed Web of Science ®Google Scholar
• Mourembou G, Fenollar F, Lekana-Douki JB, et al. Mansonella, including a potential new species, as common parasites in children in Gabon. PLoS Negl Trop Dis. 2015;9(10):e0004155. doi: 10.1371/journal.pntd.0004155
   PubMed Web of Science ®Google Scholar
• Rodi M, Gross C, Sandri TL, et al. Whole genome analysis of two sympatric human Mansonella: Mansonella perstans and Mansonella sp “DEUX”. Front Cell Infect Microbiol. 2023;13:1159814.
   PubMed Web of Science ®Google Scholar
• Lefoulon E, Bain O, Bourret J, et al. Shaking the tree: multi-locus sequence typing usurps current onchocercid (filarial nematode) phylogeny. PLoS Negl Trop Dis. 2015;9(11):e0004233. doi: 10.1371/journal.pntd.0004233
   PubMed Web of Science ®Google Scholar
• Bain O, Mutafchiev Y, Junker K, et al. review of the genus Mansonella Faust, 1929 sensu lato (Nematoda: Onchocercidae), with descriptions of a new subgenus and a new subspecies. Zootaxa. 2015;3918(2):151–193. doi: 10.11646/zootaxa.3918.2.1
   PubMed Web of Science ®Google Scholar
• Mathison BA, Couturier MR, Pritt BS, et al. Diagnostic identification and differentiation of microfilariae. J Clin Microbiol. 2019;57(10):e00706–19. doi: 10.1128/JCM.00706-19
   PubMed Web of Science ®Google Scholar
• Gazzinelli-Guimaraes PH, Nutman TB. Helminth parasites and immune regulation. F1000Res. 2018;7:1685. F1000 Faculty Rev-1685. doi: 10.12688/f1000research.15596.1
   Google Scholar
• Lima NF, Gonçalves-Lopes RM, Kruize YCM, et al. CD39 and immune regulation in a chronic helminth infection: the puzzling case of Mansonella ozzardi. PLoS Negl Trop Dis. 2018;12(3):e0006327. doi: 10.1371/journal.pntd.0006327
   PubMed Web of Science ®Google Scholar
• Ritter M, Ndongmo WPC, Njouendou AJ, et al. Mansonella perstans microfilaremic individuals are characterized by enhanced type 2 helper T and regulatory T and B cell subsets and dampened systemic innate and adaptive immune responses. PLoS Negl Trop Dis. 2018;12(1):e0006184. doi: 10.1371/journal.pntd.0006184
   PubMed Web of Science ®Google Scholar
• Wangala B, Gantin RG, Voßberg PS, et al. Inflammatory and regulatory CCL and CXCL chemokine and cytokine cellular responses in patients with patent Mansonella perstans filariasis. Clin Exp Immunol. 2019;196:111–122. doi: 10.1111/cei.13251
   PubMed Web of Science ®Google Scholar
• Ferreira MU, Crainey JL, Luz SLB. Mansonella ozzardi. Trends Parasitol. 2021;37(1):90–91. doi: 10.1016/j.pt.2020.03.005
   PubMed Web of Science ®Google Scholar
• Vianna LM, Martins M, Cohen MJ, et al. Mansonella ozzardi corneal lesions in the Amazon: a cross-sectional study. BMJ Open. 2012;2(6):e001266. doi: 10.1136/bmjopen-2012-001266
   PubMed Web of Science ®Google Scholar
• Gobbi F, Beltrame A, Buonfrate D, et al. Imported infections with Mansonella perstans nematodes, Italy. Emerg Infect Dis. 2017;23(9):1539–1542. doi: 10.3201/eid2309.170263
   PubMed Web of Science ®Google Scholar
• Fischer P, Bamuhiiga J, Büttner DW. Occurrence and diagnosis of Mansonella streptocerca in Uganda. Acta Trop. 1997;63:43–55. doi: 10.1016/s0001-706x(96)00607-9
   PubMed Web of Science ®Google Scholar
• Ta-Tang TH, Crainey JL, Post RJ, et al. Mansonellosis: current perspectives. Res Rep Trop Med. 2018;9:9–24. doi: 10.2147/RRTM.S125750
   PubMed Web of Science ®Google Scholar
• World Health Organization. Bench aids for the diagnosis of filarial infections. Geneva: World Health Organization. 1997. [Cited 2023 Apr 29]. Available from: https://apps.who.int/iris/handle/10665/37156.
   Google Scholar
• Post RJ, Adams Z, Shelley AJ, et al. The morphological discrimination of microfilariae of Onchocerca volvulus from Mansonella ozzardi. Parasitology. 2003;127(1):21–27. doi: 10.1017/s003118200300324x
   PubMedGoogle Scholar
• Ta TH, Moya L, Nguema J, et al. Geographical distribution and species identification of human filariasis and onchocerciasis in Bioko Island, Equatorial Guinea. Acta Trop. 2018;180:12–17. doi: 10.1016/j.actatropica.2017.12.030
   PubMed Web of Science ®Google Scholar
• Nana-Djeunga HC, Fossuo-Thotchum F, Pion SD, et al. Loa loa Microfilariae in skin snips: consequences for onchocerciasis monitoring and evaluation in L. loa–endemic areas. Clin Infect Dis. 2019;69(9):1628–1630. doi: 10.1093/cid/ciz172
   PubMed Web of Science ®Google Scholar
• Bamuhiiga P, Büttner DW, Fischer J, et al. Detection of the filarial parasite Mansonella streptocerca in skin biopsies by a nested polymerase chain reaction-based assay. Am J Trop Med Hyg. 1998;58(6):816–820. doi: 10.4269/ajtmh.1998.58.816
   PubMed Web of Science ®Google Scholar
• Morales-Hojas R, Post RJ, Shelley AJ, et al. Characterisation of nuclear ribosomal DNA sequences from Onchocerca volvulus and Mansonella ozzardi (Nematoda: Filarioidea) and development of a PCR-based method for their detection in skin biopsies. Int J Parasitol. 2001;31:169–177. doi: 10.1016/s0020-7519(00)00156-9
   PubMed Web of Science ®Google Scholar
• Medeiros JF, Almeida TA, Silva LB, et al. A field trial of a PCR-based Mansonella ozzardi diagnosis assay detects high-levels of submicroscopic M. ozzardi infections in both venous blood samples and FTA® card dried blood spots. Parasites Vectors. 2015;8(1):280. doi: 10.1186/s13071-015-0889-z
   PubMedGoogle Scholar
• Medeiros JF, Fontes G, Nascimento VLD, et al. Sensitivity of diagnostic methods for Mansonella ozzardi microfilariae detection in the Brazilian Amazon Region. Mem Inst Oswaldo Cruz. 2018;113(3):173–177. doi: 10.1590/0074-02760170321
   PubMed Web of Science ®Google Scholar
• Formenti F, Tang TT, Tamarozzi F, et al. Preliminary comparison between an in-house real-time PCR vs microscopy for the diagnosis of Loa loa and Mansonella perstans. Acta Trop. 2021;216:105838. doi: 10.1016/j.actatropica.2021.105838
   PubMed Web of Science ®Google Scholar
• Ta-Tang TH, Febrer-Sendra B, Berzosa P, et al. Comparison of three PCR-based methods to detect Loa loa and Mansonella perstans in long-term frozen storage dried blood spots. Trop Med Int Health. 2022;27(8):686–695. doi: 10.1111/tmi.13786
   PubMed Web of Science ®Google Scholar
• Poole CB, Sinha A, Ettwiller L, et al. In silico identification of novel biomarkers and development of new rapid diagnostic tests for the filarial parasites Mansonella perstans and Mansonella ozzardi. Sci Rep. 2019;9(1):10275. doi: 10.1038/s41598-019-46550-9
   PubMedGoogle Scholar
• Amambo GN, Innocentia N, Abong RA, et al. Application of loop mediated isothermal amplification (LAMP) assays for the detection of Onchocerca volvulus, Loa loa and Mansonella perstans in humans and vectors. Front Trop Dis. 2023;3:1016176. doi: 10.3389/fitd.2022.1016176
   PubMedGoogle Scholar
• Crainey JL, Costa CHA, de Oliveira Leles LF, et al. Deep sequencing reveals occult mansonellosis coinfections in residents from the Brazilian Amazon village of São Gabriel da Cachoeira. Clin Infect Dis. 2020;71(8):1990–1993. doi: 10.1093/cid/ciaa082
   PubMed Web of Science ®Google Scholar
• Wewer V, Peisker H, Gutbrod K, et al. Urine metabolites for the identification of Onchocerca volvulus infections in patients from Cameroon. Parasites Vectors. 2021;14(1):397. doi: 10.1186/s13071-021-04893-1
   PubMed Web of Science ®Google Scholar
• Amaral F, Dreyer G, Figueredo-Silva J, et al. Live adult worms detected by ultrasonography in human Bancroftian filariasis. Am J Trop Med Hyg. 1994;50(6):753–757. doi: 10.4269/ajtmh.1994.50.753
   PubMed Web of Science ®Google Scholar
• Mand S, Marfo-Debrekyei Y, Debrah A, et al. Frequent detection of worm movements in onchocercal nodules by ultrasonography. Filaria J. 2005;4(1):1. doi: 10.1186/1475-2883-4-1
   PubMedGoogle Scholar
• Turner JD, Tendongfor N, Esum M, et al. Macrofilaricidal activity after doxycycline only treatment of Onchocerca volvulus in an area of Loa loa co-endemicity: a randomized controlled trial. PLoS Negl Trop Dis. 2010;4(4):e660. doi: 10.1371/journal.pntd.0000660
   PubMed Web of Science ®Google Scholar
• Crainey JL, Marín MA, TRRD S, et al. Mansonella ozzardi mitogenome and pseudogene characterisation provides new perspectives on filarial parasite systematics and CO-1 barcoding. Sci Rep. 2018;8(1):6158. doi: 10.1038/s41598-018-24382-3
   PubMedGoogle Scholar
• Chung M, Aluvathingal J, Bromley RE, et al. Complete mitochondrial genome sequence of Mansonella perstans. Microbiol Resour Announc. 2020;9(30):e00490–20. doi: 10.1128/MRA.00490-20
   PubMed Web of Science ®Google Scholar
• Sinha A, Li Z, Poole CB, et al. Genomes of the human filarial parasites Mansonella perstans and Mansonella ozzardi. Front Trop Dis. 2023;4:1139343. doi: 10.3389/fitd.2023.1139343
   Google Scholar
• Bartholomew CF, Nathan MB, Tikasingh ES. The failure of diethylcarbamazine in the treatment of Mansonella ozzardi infections. Trans R Soc Trop Med Hyg. 1978;72:423–424. doi: 10.1016/0035-9203(78)90141-4
   PubMed Web of Science ®Google Scholar
• Chadee DD, Tilluckdharry CC, Rawlins SC, et al. Mass chemotherapy with diethylcarbamazine for the control of Bancroftian filariasis: A twelve-year follow-up in northern Trinidad, including observations on Mansonella ozzardi. Am J Trop Med Hyg. 1995;52(2):174–176. doi: 10.4269/ajtmh.1995.52.174
   PubMed Web of Science ®Google Scholar
• Bregani ER, Rovellini A, Mbaïdoum N, et al. Comparison of different anthelminthic drug regimens against Mansonella perstans filariasis. Trans R Soc Trop Med Hyg. 2006;100:458–463. doi: 10.1016/j.trstmh.2005.07.009
   PubMed Web of Science ®Google Scholar
• Casiraghi M, Favia G, Cancrini G, et al. Molecular identification of Wolbachia from the filarial nematode Mansonella ozzardi. Parasitol Res. 2001;87(5):417–420. doi: 10.1007/s004360000368
   PubMed Web of Science ®Google Scholar
• Keiser PB, Coulibaly Y, Kubofcik J, et al. Molecular identification of Wolbachia from the filarial nematode Mansonella perstans. Mol Biochem Parasitol. 2008;160:123–128. doi: 10.1016/j.molbiopara.2008.04.012
   PubMed Web of Science ®Google Scholar
• Sandri TL, Kreidenweiss A, Cavallo S, et al. Molecular epidemiology of Mansonella species in Gabon. J Infect Dis. 2021;223(2):287–296. doi: 10.1093/infdis/jiaa670
   PubMed Web of Science ®Google Scholar
• Grobusch MP, Kombila M, Autenrieth I, et al. No evidence of Wolbachia endosymbiosis with Loa loa and Mansonella perstans. Parasitol Res. 2003;90:405–408. doi: 10.1007/s00436-003-0872-z
   PubMed Web of Science ®Google Scholar
• Gehringer C, Kreidenweiss A, Flamen A, et al. Molecular evidence of Wolbachia endosymbiosis in Mansonella perstans in Gabon, Central Africa. J Infect Dis. 2014;210(10):1633–1638. doi: 10.1093/infdis/jiu320
   PubMed Web of Science ®Google Scholar
• Leles LF Avaliação da diversidade genética populacional e prevaléncia de infecção por Wolbachia em Mansonella ozzardi no Estado do Amazonas, Brasil [in Portuguese]. MSc Thesis, Fiocruz. 2019. [Cited 2023 Apr 5]. Available from: https://www.arca.fiocruz.br/handle/icict/32209.
   Google Scholar
• Sinha A, Li Z, Poole CB, et al. Multiple lineages of nematode-Wolbachia symbiosis in supergroup F and convergent loss of bacterioferritin in filarial Wolbachia. Genome Biol Evol. evad073. doi: 10.1093/gbe/evad073.
   PubMed Web of Science ®Google Scholar
• Pfarr KM, Hoerauf AM. Antibiotics which target the Wolbachia endosymbionts of filarial parasites: a new strategy for control of filariasis and amelioration of pathology. Mini Rev Med Chem. 2006;6:203–210. doi: 10.2174/138955706775475984
   PubMed Web of Science ®Google Scholar
• Slatko BE, Luck AN, Dobson SL, et al. Wolbachia endosymbionts and human disease control. Mol Biochem Parasitol. 2014;195:88–95. doi: 10.1016/j.molbiopara.2014.07.004
   PubMed Web of Science ®Google Scholar
• Ehrens A, Hoerauf A, Hübner MP. Current perspective of new anti-wolbachial and direct-acting macrofilaricidal drugs as treatment strategies for human filariasis. GMS Infect Dis. 2022;10:Doc02. doi: 10.3205/id000079
   PubMedGoogle Scholar
• Ferri E, Bain O, Barbuto M, et al. New insights into the evolution of Wolbachia infections in filarial nematodes inferred from a large range of screened species. PLoS One. 2011;6(6):e20843. doi: 10.1371/journal.pone.0020843
   PubMed Web of Science ®Google Scholar
• Nutman TB, Nash TE, Ottesen EA. Ivermectin in the successful treatment of a patient with Mansonella ozzardi infection. J Infect Dis. 1987;156(4):662–665. doi: 10.1093/infdis/156.4.662
   PubMed Web of Science ®Google Scholar
• Basano SA, Fontes G, Medeiros JF, et al. Sustained clearance of Mansonella ozzardi infection after treatment with ivermectin in the Brazilian Amazon. Am J Trop Med Hyg. 2014;90(6):1170–1175. doi: 10.4269/ajtmh.13-0410
   PubMed Web of Science ®Google Scholar
• de Almeida Basano S, de Souza Almeida Aranha Camargo J, Fontes G, et al. Phase III clinical trial to evaluate ivermectin in the reduction of Mansonella ozzardi infection in the Brazilian Amazon. Am J Trop Med Hyg. 2018;98(3):786–790. doi: 10.4269/ajtmh.17-0698
   PubMed Web of Science ®Google Scholar
• Restrepo M, Ochoa N. Tratamiento com levamisol de la infeccion por Mansonella ozzardi. Rev Inst Med Trop Sao Paulo. 1986;28(2):104–110. doi: 10.1590/s0036-46651986000200007
   PubMed Web of Science ®Google Scholar
• Van Hoegaerden M, Ivanoff B, Flocard F, et al. The use of mebendazole in the treatment of filariases due to Loa loa and Mansonella perstans. Ann Trop Med Parasitol. 1987;81:275–282. doi: 10.1080/00034983.1987.11812120
   PubMedGoogle Scholar
• Maertens K, Wéry M. Effect of mebendazole and levamisole on Onchocerca volvulus and Dipetalonema perstans. Trans R Soc Trop Med Hyg. 1975;69:359–360. doi: 10.1016/0035-9203(75)90134-0
   PubMed Web of Science ®Google Scholar
• Duong TH, Kombila M, Ferrer A, et al. Decrease in Mansonella perstans microfilaraemia after albendazole treatment. Trans R Soc Trop Med Hyg. 1998;92:459. doi: 10.1016/s0035-9203(98)91093-8
   PubMed Web of Science ®Google Scholar
• Van den Enden E, Van Gompel A, Vervoort T, et al. Mansonella perstans filariasis: failure of albendazole treatment. Ann Soc Belg Med Trop. 1992;72(3):215–218.
   PubMedGoogle Scholar
• Van den Enden E, Van Gompel A, Van der Stuyft P, et al. Treatment failure of a single high dose of ivermectin for Mansonella perstans filariasis. Trans R Soc Trop Med Hyg. 1993;87:90. doi: 10.1016/0035-9203(93)90438-v
   PubMed Web of Science ®Google Scholar
• Asio SM, Simonsen PE, Onapa AW. A randomised, double-blind field trial of ivermectin alone and in combination with albendazole for the treatment of Mansonella perstans infections in Uganda. Trans R Soc Trop Med Hyg. 2009;103:274–279. doi: 10.1016/j.trstmh.2008.10.038
   PubMed Web of Science ®Google Scholar
• Asio SM, Simonsen PE, Onapa AW. Mansonella perstans: safety and efficacy of ivermectin alone, albendazole alone and the two drugs in combination. Ann Trop Med Parasitol. 2009;103:31–37. doi: 10.1179/136485909X384929
   PubMed Web of Science ®Google Scholar
• Gardon J, Kamgno J, Gardon-Wendel N, et al. Efficacy of repeated doses of ivermectin against Mansonella perstans. Transactions Of The Royal Society Of Tropical Medicine And Hygiene. 2002;96(3):325–326. doi: 10.1016/s0035-9203(02)90112-4
   PubMed Web of Science ®Google Scholar
• Wanji S, Tayong DB, Layland LE, et al. Update on the distribution of Mansonella perstans in the southern part of Cameroon: influence of ecological factors and mass drug administration with ivermectin. Parasites Vectors. 2016;9(1):311. doi: 10.1186/s13071-016-1595-1
   PubMedGoogle Scholar
• Francis H, Awadzi K, Ottesen EA. The Mazzotti reaction following treatment of onchocerciasis with diethylcarbamazine: clinical severity as a function of infection intensity. Am J Trop Med Hyg. 1985;34(3):529–536. doi: 10.4269/ajtmh.1985.34.529
   PubMed Web of Science ®Google Scholar
• Fordjour FA, Kwarteng A. The filarial and the antibiotics: Single or combination therapy using antibiotics for filariasis. Front Cell Infect Microbiol. 2022;12:1044412. doi: 10.3389/fcimb.2022.1044412
   PubMed Web of Science ®Google Scholar
• Coulibaly YI, Dembele B, Diallo AA, et al. A randomized trial of doxycycline for Mansonella perstans infection. N Engl J Med. 2009;361(15):1448–1458. doi: 10.1056/NEJMoa0900863
   PubMed Web of Science ®Google Scholar
• Batsa Debrah L, Phillips RO, Pfarr K, et al. The efficacy of doxycycline treatment on Mansonella perstans infection: an open-label, randomized trial in Ghana. Am J Trop Med Hyg. 2019;101(1):84–92. doi: 10.4269/ajtmh.18-0491
   PubMed Web of Science ®Google Scholar
• Puente S, Lago M, Subirats M, et al. Imported Mansonella perstans infection in Spain. Infect Dis Poverty. 2020;9(1):105. doi: 10.1186/s40249-020-00729-9
   PubMed Web of Science ®Google Scholar
• Bottieau E, Huits R, Van Den Broucke S, et al. Human filariasis in travelers and migrants: a retrospective 25-year analysis at the Institute of Tropical Medicine, Antwerp. Belgium Clin Infect Dis. 2022;74(11):1972–1978. doi: 10.1093/cid/ciab751
   PubMed Web of Science ®Google Scholar
• Tamarozzi F, Rodari P, Salas-Coronas J, et al. A large case series of travel-related Mansonella perstans (vector-borne filarial nematode): a TropNet study in Europe. J Travel Med. 2022;29:taac048. doi: 10.1093/jtm/taac048
   PubMed Web of Science ®Google Scholar
• Gobbi F, Tamarozzi F, Buonfrate D, et al. Laboratory parameters after treatment for Loa loa and Mansonella perstans: the experience of a single referral center for tropical diseases in a non-endemic area. Am J Trop Med Hyg. 2019;100:914–920. doi: 10.4269/ajtmh.18-0693
   PubMed Web of Science ®Google Scholar
• Meyers WM, Connor DH, Harman LE, et al. Human streptocerciasis. A clinico-pathologic study of 40 Africans (Zairians) including identification of the adult filaria. Am J Trop Med Hyg. 1972;21(5):528–545. doi: 10.4269/ajtmh.1972.21.528
   PubMed Web of Science ®Google Scholar
• Meyers WM, Moris R, Neafie RC, et al. Streptocerciasis: Degeneration of adult dipetalonema streptocerca in man following diethylcarbamazine therapy. The American Journal Of Tropical Medicine And Hygiene. 1978;27(6):1137–1147. doi: 10.4269/ajtmh.1978.27.1137
   PubMed Web of Science ®Google Scholar
• Fischer P, Tukesiga E, Büttner DW. Long-term suppression of Mansonella streptocerca microfilariae after treatment with ivermectin. J Infect Dis. 1999;180(4):1403–1405. doi: 10.1086/315014
   PubMed Web of Science ®Google Scholar
• Little MP, Breitling LP, Basáñez MG, et al. Association between microfilarial load and excess mortality in onchocerciasis: an epidemiological study. Lancet. 2004;363(9420):1514–1521. doi: 10.1016/S0140-6736(04)16151-5
   PubMed Web of Science ®Google Scholar
• Chesnais CB, Takougang I, Paguélé M, et al. Excess mortality associated with loiasis: a retrospective population-based cohort study. Lancet Infect Dis. 2017;17:108–116. doi: 10.1016/S1473-3099(16)30405-4
   PubMed Web of Science ®Google Scholar
• Veletzky L, Hergeth J, Stelzl DR, et al. Burden of disease in Gabon caused by loiasis: a cross-sectional survey. Lancet Infect Dis. 2020;20:1339–1346. doi: 10.1016/S1473-3099(20)30256-5
   PubMed Web of Science ®Google Scholar
• Jacobsen KH, Andress BC, Bhagwat EA, et al. A call for loiasis to be added to the WHO list of neglected tropical diseases. Lancet Infect Dis. 2022;22:e299–e302. doi: 10.1016/S1473-3099(22)00064-0
   PubMed Web of Science ®Google Scholar
• Gonzalez AE, Codd EE, Horton J, et al. Oxfendazole: a promising agent for the treatment and control of helminth infections in humans. Expert Rev Anti Infect Ther. 2019;17:51–56. doi: 10.1080/14787210.2018.1555241
   PubMed Web of Science ®Google Scholar
• Krücken J, Holden-Dye L, Keiser J, et al. Development of emodepside as a possible adulticidal treatment for human onchocerciasis—The fruit of a successful industrial–academic collaboration. PLOS Pathog. 2021;17(7):e1009682. doi: 10.1371/journal.ppat.1009682
   PubMed Web of Science ®Google Scholar
• Aljayyoussi G, Tyrer HE, Ford L, et al. Short-course, high-dose rifampicin achieves Wolbachia depletion predictive of curative outcomes in preclinical models of lymphatic filariasis and onchocerciasis. Sci Rep. 2017;7(1):210. doi: 10.1038/s41598-017-00322-5
   PubMedGoogle Scholar
• Volkmann L, Fischer K, Taylor M, et al. Antibiotic therapy in murine filariasis (Litomosoides sigmodontis): comparative effects of doxycycline and rifampicin on Wolbachia and filarial viability. Trop Med Int Health. 2003;8(5):392–401. doi: 10.1046/j.1365-3156.2003.01040.x
   PubMed Web of Science ®Google Scholar