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Natural Product Research
Formerly Natural Product Letters
Volume 30, 2016 - Issue 20
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Short Communication

Preparation of highly purified timosaponin AIII from rhizoma anemarrhenae through an enzymatic method combined with preparative liquid chromatography

Lu LuSchool of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
Yanping LiuSchool of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
Yue DingExperiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
Jianwei HouExperiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaCorrespondence[email protected]
,
Yong ZhangExperiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
Haiping XueExperiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, China
&
Tong ZhangExperiment Center for Teaching and Learning, Shanghai University of Traditional Chinese Medicine, Shanghai, ChinaCorrespondence[email protected]
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Pages 2364-2367 | Received 05 Dec 2015, Accepted 06 Mar 2016, Published online: 07 Apr 2016

References

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  • Li SY, Zhao ZH, Pei HY, Xiong CQ, Han Y, Ma BP, Mao BZ, Cong YW. 2006. Effect of timosaponin-AIII on formation of thrombus. Bull Acad Mil Med Sci. 30:340–342.
  • Liang L, Bian B, Wang H. 2010. Determination of mangiferin and timosaponin BII in anemarrhenae rhizoma from different areas. Chin J Exp Tradit Med Formulae. 16:49–51.
  • Lim SM, Jeong JJ, Kang GD, Kim KA, Choi HS, Kim DH. 2015. Timosaponin AIII and its metabolite sarsasapogenin ameliorate colitis in mice by inhibiting NF-κB and MAPK activation and restoring Th17/Treg cell balance. Int Immunopharmacol. 25:493–503.10.1016/j.intimp.2015.02.016
  • Ouyang H, Huang X, Zhou M, Tu S, Xu W, Yang S. 2011. Preparation and identification of timosaponinA-III and timosaponin C. China Phar. 22:1391–1393.
  • The National Pharmacopoeia Committee. 2010. Pharmacopoeia of People’s Republic of China. Beijing: Chemical Industry Press. Part 1; p. 197–198.
  • Tsai CH, Yang CW, Wang JY, Tsai YF, Tseng LM, King KL, Chen WS, Chiu JH, Shyr YM. 2013. Timosaponin AIII suppresses hepatocyte growth factor-induced invasive activity through sustained ERK activation in breast cancer MDA-MB-231 cells. J Evidence-Based Complementary Altern Med. 20:243–253.
  • Wang N, Feng Y, Zhu M, Siu FM, Ng KM, Che CM. 2013. A novel mechanism of XIAP degradation induced by timosaponin AIII in hepatocellular carcinoma. Biochim Biophys Acta. 1833:2890–2899.10.1016/j.bbamcr.2013.07.018
  • Yuan Y, Chen W, Sun L, Zheng S, Lou Z, Zhang H. 2006. Determination of the content of saponins in commercial herbs of rhizoma anemarrhenae. Chin J Hosp Pharm. 26:656–658.
  • Zhou WB, Feng B, Huang HZ, Qin YJ, Wang YZ, Kang LP, Zhao Y, Wang XN, Cai Y, Tan DW, Ma BP. 2010. Enzymatic synthesis of α-glucosyl-timosaponin BII catalyzed by the extremely thermophilic enzyme: toruzyme 3.0L. Carbohydr Res. 345:1752–1759.10.1016/j.carres.2010.05.027

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