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Original Articles

An Enzymatic Transglycosylation of Purine Bases

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Pages 905-909 | Published online: 10 Dec 2007

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  • Standard reaction conditions: the donor-acceptor ratio was 1.5–3:1 (mol); 5 mM K-phosphate buffer (pH 7.0); heterogeneous reaction mixture was gentle stirred at 50 °C monitoring the progress of reaction by TLC and HPLC; after the reaction reached constant equilibrium, SiO2 was added and the mixture was evaporated to dryness, residue co-evaporated twice with abs EtOH, and SiO2 with products was placed on the top of a column packed with SiO2 in a mixture of CH2Cl2-MeOH (95:5, vol). Elution with a linear gradient of MeOH in CH2Cl2-MeOH (95:5, vol) gave the desired nucleosides

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