Phytochemical profile and determination of cytotoxicity, acute oral toxicity, genotoxicity, and mutagenicity of aqueous and ethanolic extracts of Pseudobombax marginatum (A. St.-Hil.) A. Robyns

References

• Al-Majedy, Y., A. Al-Amiery, A. A. E. Kadhum, and A. BakarMohamad. 2017. “Antioxidant Activity of Coumarins.” Systematic Reviews in Pharmacy 8 (1): 24–30. https://doi.org/10.5530/srp.2017.1.6.
   Google Scholar
• Aquino, V. V. F., J. G. M. Costa, E. C. Angélico, R. S. Medeiros, M. D. F. A. Lucena, and O. G. Rodrigues. 2017. “Metabólitos Secundários e ação antioxidante de Croton helliotripifolius e Croton blanchetianus.” Acta Brasiliensis 1 (3): 28–31. https://doi.org/10.22571/Actabra13201730.
   Google Scholar
• Araújo, M. C. D. P. M., N. M. S. Barcellos, P. M. de Abreu Vieira, T. M. Gouveia, M. O. Guerra, V. M. Peters, and D. A. Saúde-Guimarães. 2017. “Acute and Sub Chronic Toxicity Study of Aqueous Extract from the Leaves and Branches of Campomanesia Velutina (Cambess) O. Berg.” Journal of Ethnopharmacology 201:17–25. https://doi.org/10.1016/j.jep.2017.02.043.
   PubMed Web of Science ®Google Scholar
• Behmer, O. A., and E. M. C. D. Tolosa. 2003. “Manual de técnicas para histologia normal e patológica.” Manual de Técnicas Para Histologia Normal ePatológica 331–331. https://pesquisa.bvsalud.org/portal/resource/pt/biblio-924705.
   Google Scholar
• Campelo, L. M. L., C. G. Sá, C. M. Feitosa, G. F. Sousa, and R. M. Freitas. 2013. “Constituintes químicos e estudos toxicológicos do óleo essencial extraído das folhas de Citrus limon Burn (Rutaceae).” Revista Brasileira de Plantas Medicinais 15 (4): 708–716. https://doi.org/10.1590/S1516-05722013000500011.
   Google Scholar
• Chen, H., J. Pu, D. Liu, W. Yu, Y. Shao, G. Yang, and N. He. 2016. “Anti-Inflammatory and Antinociceptive Properties of Flavonoids from the Fruits of Black Mulberry (Morus nigra L.).” Public Library of Science ONE 11 (4): e0153080. https://doi.org/10.1371/journal.pone.0153080.
   Google Scholar
• Collins, A. R., A. A. Oscoz, G. Brunborg, I. Gaivao, L. Giovannelli, M. Kruszewski, C. C. Smith, and R. Stetina. 2008. “The Comet Assay: Topical Issues.” Mutagenesis 23 (3): 143–151. https://doi.org/10.1093/mutage/gem051.
   PubMed Web of Science ®Google Scholar
• da Silva, M. M., T. A. Rocha, D. F. de Moura, C. A. Chagas, F. C. A. de Aguiar Júnior, N. P. da Silva Santos, R. V. Da Silva Sobral, et al. 2019. “Effect of Acute Exposure in Swiss Mice (Mus musculus) to a Fibrinolytic Protease Produced by Mucor Subtilissimus UCP 1262: An Histomorphometric, Genotoxic and Cytological Approach.” Regulatory Toxicology and Pharmacology: RTP 103:282–291. https://doi.org/10.1016/j.yrtph.2019.02.009.
   PubMed Web of Science ®Google Scholar
• de Barros, M. C., A. G. B. Silva, T. G. dos Santos Souza, C. A. Chagas, J. C. B. Machado, M. R. A. Ferreira, and P. M. G. Paiva. 2022. “Evaluation of Acute Toxicity, 28-Day Repeated Dose Toxicity, and Genotoxicity of Moringa Oleifera Leaves Infusion and Powder.” Journal of Ethnopharmacology 296:115504. https://doi.org/10.1016/j.jep.2022.115504.
   PubMed Web of Science ®Google Scholar
• de Brito Costa, E. M. M., A. S. Barbosa, V. G. B. Florentino, J. D. F. da Silva, D. M. D. B. M. Trovão, and A. C. D. De Medeiros. 2014. “In Vitro Antimicrobial Activity of Plant Extracts of Semi-Arid Region of Paraíba, PB, Brazil. Ver.” Odonto Ciênc 28 (4): 101–104.
   Google Scholar
• de Melo Alcântara, L. F., P. T. da Silva, Q. M. de Oliveira, T. G. dos Santos Souza, M. M. da Silva, G. S. Feitoza, M. V. da Silva, et al. 2023. “Toxicological Safety, Antioxidant Activity and Phytochemical Characterization of Leaf and Bark Aqueous Extracts of Commiphora Leptophloeos (Mart.) J.B. Gillett.” Journal of Toxicology and Environmental Health Part A 86 (16): 557–574. https://doi.org/10.1080/15287394.2023.2224827.
   PubMed Web of Science ®Google Scholar
• de Menezes, M. A. G., F. B. de Oliveira Neto, L. M. Bertino, F. F. M. da Silva, and L. A. Alves. 2015. “Quantificação de antocianinas dos extratos de Embiratanha (Pseudobombax marginatum).” HOLOS 1:30–35. https://doi.org/10.15628/holos.2015.2459.
   Google Scholar
• de Moura, D. F., T. A. Rocha, D. de Melo Barros, M. M. da Silva, M. A. D. C. de Lira, T. G. dos Santos Souza, C. J. A. da Silva, et al. 2020. “Evaluation of the Cytotoxicity, Oral Toxicity, Genotoxicity, and Mutagenicity of the Latex Extracted from Himatanthus Drasticus (Mart.) Plumel (Apocynaceae).” Journal of Ethnopharmacology 253:112567. https://doi.org/10.1016/j.jep.2020.112567.
   PubMed Web of Science ®Google Scholar
• De Oliveira, A. M., M. D. S. Mesquita, G. C. da Silva, E. de Oliveira Lima, P. L. de Medeiros, P. M. G. Paiva, T. H. Napoleão, and T. H. Napoleão. 2015. “Evaluation of Toxicity and Antimicrobial Activity of an Ethanolic Extract from Leaves of Morus Alba L. (Moraceae).” Evidence-Based Complementary and Alternative Medicine 2015:1–7. https://doi.org/10.1155/2015/513978.
   Web of Science ®Google Scholar
• De Quadros, A. P. O., I. B. Baraldi, M. Petreanu, R. Niero, M. S. Mantovani, I. O. N. De Mascarenhas Gaivão, and E. L. Maistro. 2023. “Cytogenotoxic Evaluations of Leaves and Stems Extracts of Rubus Rosifolius in Primary Metabolically Noncompetent Cells.” Journal of Toxicology and Environmental Health Part A 86 (11): 361–371. https://doi.org/10.1080/15287394.2023.2203190.
   PubMedGoogle Scholar
• de Vargas, F. S., P. Almeida, A. P. A. de Boleti, M. M. Pereira, T. P. de Souza, M. C. de Vasconcellos, and E. S. Lima. 2016. “Antioxidant Activity and Peroxidase Inhibition of Amazonian Plants Extracts Traditionally Used As Anti-Inflammatory.” BMC Complementary and Alternative Medicine 16 (1): 1–8. https://doi.org/10.1186/s12906-016-1061-9.
   PubMedGoogle Scholar
• Dos Santos Souza, T. G., M. M. da Silva, G. S. Feitoza, L. F. de Melo Alcântara, M. A. da Silva, A. M. de Oliveira, J. C. R. O. Farias de Aguiar, et al. 2021. “Biological Safety of Syagrus coronata (Mart.) Becc. Fixed Oil: Cytotoxicity, Acute Oral Toxicity, and Genotoxicity Studies.” Journal of Ethnopharmacology 272:113941. https://doi.org/10.1016/j.jep.2021.113941.
   PubMed Web of Science ®Google Scholar
• Efferth, T., and F. Oesch. 2021. “Repurposing of Plant Alkaloids for Cancer Therapy: Pharmacology and Toxicology.” Seminars in Cancer Biology 68:143–163. https://doi.org/10.1016/j.semcancer.2019.12.010.
   PubMed Web of Science ®Google Scholar
• Ferreira, P. M. P., D. D. R. Arcanjo, and A. P. Perón. 2023. “Drug Development, Brazilian Biodiversity and Political Choices: Where Are We Heading?” Journal of Toxicology and Environmental Health, Part B 26 (5): 257–274. https://doi.org/10.1080/10937404.2023.2193762.
   PubMed Web of Science ®Google Scholar
• Figueiredo, S. S., F. K. D. S. Monteiro, and J. I. M. D. Melo. 2020. “Flora of Paraíba, Brazil: Bombacoideae burnett (Malvaceae).” Biota Neotropica 20 (2). https://doi.org/10.1590/1676-0611-BN-2019-0837.
   Web of Science ®Google Scholar
• Freitas, A. V. L., and M. D. F. B. Coelho. 2014. “Os ‘remédios do mato’ por especialistas locais da comunidade São João da Várzea, Mossoró, RN, Brasil.” Interações (Campo Grande) 15:249–264. https://doi.org/10.1590/S1518-70122014000200005.
   Google Scholar
• Gontijo, D. C., L. C. Fietto, and J. P. V. Leite. 2014. “Avaliação fitoquímica e atividade antioxidan e, antimutagênica e toxicológica do extrato aquoso das folhas de Ocimum gratissimum L.” Revista Brasileira de Plantas Medicinais 16 (4): 874–880. https://doi.org/10.1590/1983-084X/12_002.
   Google Scholar
• Hayashi, M., T. Morita, Y. Kodama, T. Sofuni, and M. Ishidate. 1990. “The Micronucleus Assay with Mouse Peripheral Blood Reticulocytes Using Acridine Orange-Coated Slides.” Mutation Research 245:245–249. https://doi.org/10.1016/0165-7992(90)90153-B.
   PubMed Web of Science ®Google Scholar
• Hougee, S., A. Sanders, J. Faber, Y. M. Graus, W. B. Vanden, B. J. Garssen, H. F. Smit, and M. A. Hoijer. 2005. “Decreased Pro-Inflammatory Cytokine Production by LPS-Stimulated PBMC Upon in vitro Incubation with the Flavonoids Apigenin, Luteolin or Chrysin, Due to Selective Elimination of Monocytes/Macrophages.” Biochemical Pharmacology 69 (2): 241–248. https://doi.org/10.1016/j.bcp.2004.10.002.
   PubMed Web of Science ®Google Scholar
• Kessin, J. P., L. Pigozzi, P. C. Hahn, G. V. Dias, M. R. Nunes, M. Dalla Costa, and A. P. de Lima Veeck. 2018. “Atividade antioxidante de compostos fenólicos presentes em polpa e casca de goiabeira serrana.” Brazilian Journal of Food Research 9 (1): 141–153. https://doi.org/10.3895/rebrapa.v9n1.5195.
   Google Scholar
• Maisetta, G., G. Batoni, P. Caboni, S. Esin, A. C. Rinaldi, and P. Zucca. 2019. “Tannin Profile, Antioxidant Properties, and Antimicrobial Activity of Extracts from Two Mediterranean Species of Parasitic Plant Cytinus.” BMC Complementary and Alternative Medicine 19:82. https://doi.org/10.1186/s12906-019-2487-7.
   PubMed Web of Science ®Google Scholar
• Mendonça, E. D., J. da Silva, M. S. Dos Santos, P. Carvalho, D. K. M. Papke, C. F. Ortmann, and A. D. B. F. Ferraz. 2016. “Genotoxic, Mutagenic and Antigenotoxic Effects of Cecropia Pachystachya Trécul Aqueous Extract Using in Vivo and in Vitro Assays.” Journal of Ethnopharmacology 193:214–220. https://doi.org/10.1016/j.jep.2016.07.046.
   PubMed Web of Science ®Google Scholar
• Miya, G. M., A. O. Oriola, B. Payne, M. Cuyler, N. Lall, and A. O. Oyedeji. 2023. “Steroids and Fatty Acid Esters from Cyperus Sexangularis Leaf and Their Antioxidant, Anti-Inflammatory and Anti-Elastase Properties.” Molecules 28 (8): 3434. https://doi.org/10.3390/molecules28083434.
   PubMed Web of Science ®Google Scholar
• Moraes, G. V., G. M. Jorge, R. V. Gonzaga, and D. A. dos Santos. 2022. “Potencial antioxidante dos flavonoides e aplicações terapêuticas.” Research, Society and Development 11 (14): e238111436225–e238111436225. https://doi.org/10.33448/rsd-v11i14.36225.
   Google Scholar
• Mosmann, T. 1983. “Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays.” Journal of Immunological Methods 65 (1–2): 55–63. https://doi.org/10.1016/0022-1759(83)90303-4.
   PubMed Web of Science ®Google Scholar
• Nazari, S., M. Rameshrad, and H. Hosseinzadeh. 2017. “Toxicological Effects of Glycyrrhiza Glabra (Licorice): A Review.” Phytotherapy Research: PTR 31 (11): 1635–1650. https://doi.org/10.1002/ptr.5893.
   PubMed Web of Science ®Google Scholar
• Ndayambaje, M., H. Wahnou, M. Sow, O. Chgari, T. Habyarimana, M. Karkouri, and M. Oudghiri. 2024. “Exploring the Multifaceted Effects of Ammi visnaga: Subchronic Toxicity, Antioxidant Capacity, Immunomodulatory, and Anti-Inflammatory Activities.” Journal of Toxicology and Environmental Health Part A 87 (4): 150–165. https://doi.org/10.1080/15287394.2023.2289430.
   PubMed Web of Science ®Google Scholar
• Niero, E. L. D. O., and G. M. Machado-Santelli. 2013. “Cinnamic Acid Induces Apoptotic Cell Death and Cytoskeleton Disruption in Human Melanoma Cells.” Journal of Experimental & Clinical Cancer Research 32 (1): 1–14. https://doi.org/10.1186/1756-9966-32-31.
   PubMedGoogle Scholar
• OECD. 2001. “Test No. 423: Acute Oral Toxicity - Acute Toxic Class Method.”
   Google Scholar
• OECD. 2016a. “Test No. 474: Mammalian Erythrocyte Micronucleus.”
   Google Scholar
• OECD. 2016b. “Test No. 489: in Vivo Mammalian Alkaline Comet Assay.”
   Google Scholar
• Paiva, D. C. C., C. A. dos Santos, J. C. Diniz, F. A. Viana, S. M. Thomazzi, and D. A. Falcão. 2013. “Anti-Inflammatory and Antinociceptive Effects of Hydroalcoholic Extract from Pseudobombax Marginatum Inner Bark from Caatinga Potiguar.” Journal of Ethnopharmacology 149 (2): 416–421. https://doi.org/10.1016/j.jep.2013.06.012.
   PubMed Web of Science ®Google Scholar
• Paulino, R., P. Araújo, G. Henriques, and M. Coelho. 2011. “Riqueza e importância das plantas medicinais do Rio Grande do Norte.” Reviews Biology Ciênt Terra 11:157–168. http://memoria.ifrn.edu.br/handle/1044/393.
   Google Scholar
• Pereira Júnior, L. R., A. P. D. Andrade, K. D. Araújo, A. D. S. Barbosa, and F. M. Barbosa. 2014. “Espécies da Caatinga como alternativa para o desenvolvimento de novos fitofármacos.” Floresta e Ambiente 21 (4): 509–520. https://doi.org/10.1590/2179-8087.024212.
   Google Scholar
• Pitarô, M., N. Croce, V. Gallo, A. Arienzo, G. Salvatore, and G. Antonini. 2022. “Coumarin-Induced Hepatotoxicity: A Narrative Review.” Molecules 27 (24): 9063. https://doi.org/10.3390/molecules27249063.
   PubMed Web of Science ®Google Scholar
• Pontiki, E., D. Hadjipavlou-Litina, K. Litinas, and G. Geromichalos. 2014. “Novel Cinnamic Acid Derivatives As Antioxidant and Anticancer Agents: Design, Synthesis and Modeling Studies.” Molecules 19 (7): 9655–9674. https://doi.org/10.3390/molecules19079655.
   PubMed Web of Science ®Google Scholar
• Rieder, N., and K. Schmidt. 1987. Morphologische Arbeitsmethoden in der Biologie. Germany: Verlagsgesellschaft mbH.
   Google Scholar
• Rocha, T. A., D. F. De Moura, M. M. Da Silva, T. G. D. S. Souza, M. A. D. C. de Lira, D. D. M. Barros, A. G. Da Silva. 2019. “Evaluation of Cytotoxic Potential, Oral Toxicity, Genotoxicity, and Mutagenicity of Organic Extracts of Pityrocarpa moniliformis.” Journal of Toxicology and Environmental Health Part A 82 (3): 216–231. https://doi.org/10.1080/15287394.2019.1576563.
   PubMed Web of Science ®Google Scholar
• Roque, A. A., R. M. Rocha, and M. I. B. Loiola. 2010. “Uso e diversidade de plantas medicinais da Caatinga na comunidade rural de Laginhas, município de Caicó, Rio Grande do Norte (Nordeste do Brasil).” Revista Brasileira de Plantas Medicinais 12 (1): 31–42. https://doi.org/10.1590/S1516-05722010000100006.
   Google Scholar
• Santos, E. C. G., C. L. Donnici, E. R. S. Camargos, G. Araújo-Silva, F. H. Xavier-Junior, and L. M. Farias. 2018. “Interaction of Pseudobombax Marginatum Robyns Stem Bark Extract on the Cell Surface of Bacillus cereus and Staphylococcus Aureus.” Journal Bacteriology Mycologie 5 (2): 1063.
   Google Scholar
• Shan, M., S. Yu, H. Yan, P. Chen, L. Zhang, and A. Ding. 2016. “A Review of the Botany, Phytochemistry, Pharmacology and Toxicology of Rubiae Radix Et Rhizoma.” Molecules 21 (12): 1747. https://doi.org/10.3390/molecules21121747.
   PubMed Web of Science ®Google Scholar
• Silva, M. L. C., R. S. Costa, A. dos Santos Santana, and M. G. B. Koblitz. 2010. “Compostos fenólicos, carotenóides e atividade antioxidante em produtos vegetais.” Semina: Ciências Agrárias 31 (3): 669–681. ISSN: 1676-546X. https://doi.org/10.5433/1679-0359.2010v31n3p669.
   Google Scholar
• Silva, T. C. L., I. Souza, E. C. Araújo, E. L. C. Amorim, T. Gomes, and J. Veras Filho. 2008. “Estudo da toxicidade subcrônica de Phyllanthus tenellus Roxb.: avaliação comportamental.” Journal of Nursing UFPE Online 2 (1): 17–22. https://doi.org/10.5205/0201200803.
   Google Scholar
• Silva, C. A., J. H. Véras, J. A. Ventura, A. V. de Melo Bisneto, M. G. de Oliveira, E. F. L. Cardoso Bailão, and L. Chen-Chen. 2023. “Chemopreventive Effect and Induction of DNA Repair by Oenothein B Ellagitannin Isolated from Leaves of Eugenia Uniflora in Swiss Webster Treated Mice.” Journal of Toxicology and Environmental Health Part A 86 (24): 929–941. https://doi.org/10.1080/15287394.2023.2259425.
   PubMed Web of Science ®Google Scholar
• Sousa Coelho, I. D. D., C. J. C. de Lapa Neto, T. G. Souza, S. dos, M. A. Silva, C. A. da Chagas, K. R. P. dos Santos, V. Wanderley Teixeira, and A. A. C. Teixeira. 2018. “Protective Effect of Exogenous Melatonin in Rats and Their Offspring on the Genotoxic Response Induced by the Chronic Consumption of Alcohol During Pregnancy.” Mutation Research/Genetic Toxicology and Environmental Mutagenesis 832-833:52–60. https://doi.org/10.1016/j.mrgentox.2018.06.018.
   PubMed Web of Science ®Google Scholar
• Tice, R. R., E. Agurell, D. Anderson, B. Burlinson, A. Hartmann, H. Kobayashi, Y. Miyamae, E. Rojas, J. C. Ryu, and Y. F. Sasaki. 2000. “Single Cell Gel/Comet Assay: Guidelines for In Vitro and In Vivo Genetic Toxicology Testing.” Environmental and Molecular Mutagenesis 35 (3): 206–221. https://doi.org/10.1002/(SICI)1098-2280(2000)35:3206::AID-EM83.0.CO;2-J.
   PubMed Web of Science ®Google Scholar
• Torres-Martínez, R., Y. M. García-Rodríguez, P. Ríos-Chávez, A. Saavedra-Molina, J. E. López-Meza, A. Ochoa-Zarzosa, and R. S. Garciglia. 2017. “Antioxidant Activity of the Essential Oil and Its Major Terpenes of Satureja Macrostema (Moc. and Sessé Ex Benth.) Briq.” Revista Farmacognosy 13 (Suplemento 4): S875. https://doi.org/10.4103/pm.pm_316_17.
   Google Scholar