Isolation of hCG and its Characterization by Radioimmunoassay, Enzyme‐Immunoassay, and Radio‐Receptor Assay

References

• Aschheim , S. and Zondek , B. 1927 . Hypophysenvorderlappenhormon und Ovarialhormon im Harm von Schwangeren . Klin. Wochenschr. , 6 : 1322 – 1325 .
   Google Scholar
• Got , R. and Bourrillon , R. 1960 . Criteria for purity of human chorionic gonadotropin . Bull. Soc. Chim. Biol. (Paris) , 42 : 31 – 39 .
   Google Scholar
• Got , R. and Bourrillon , R. 1960 . New physical data on human chorionic gonadotropin . Biochim. Biophys. Acta , 39 : 241 – 247 .
   Google Scholar
• Reisfeld , R. A. and Hertz , R. 1960 . Purification of chorionic gonadotropin from the urine of patients with trophoblastic tumors . Biochim. Biophys. Acta , 43 : 540 – 543 .
   Google Scholar
• Borth , R. , Lunenfeld , B. and Menzi , A. 1961 . Human Pituitary Gonadotropins Edited by: Albert , A. 13 Springfield, IL : Charles Thomas .
   Google Scholar
• Albert , A. 1961 . Human Pituitary Gonadotropins, Workshop Conference Edited by: Albert . Springfield, IL : Charles Thomas .
   Google Scholar
• Wilde , C. E. and Bagshawe , K. D. 1965 . Gonadotropins Edited by: Wolsten Holme , G. E.W. and Knight , J. 46 Boston : Little Brown and Company . Ciba Foundation Study Group No. 22
   Google Scholar
• van Hell , H. , Goverde , B. C. , Schuurs , A. H. , de Jager , E. , Matthijsen , R. and Homan , J. D. 1966 . Purification, characterization and immunochemical properties of human chorionic gonadotropin . Nature , 212 ( 59 ) : 261 – 262 .
   Google Scholar
• van Hell , H. , Matthijsen , R. and Homan , J. D. 1968 . Studies on human chorionic gonadotropin. I. Purification and some physico‐chemical properties . Acta Endocrinol. , 59 ( 1 ) : 89 – 104 .
   Google Scholar
• Bell , J. J. , Canfield , R. E. and Sciarra , J. J. 1969 . Purification and characterization of human chorionic gonadotropin . Endocrinology , 84 ( 2 ) : 298 – 307 .
   Google Scholar
• Matsuura , S. , Shimizu , T. , Oh , S. , Ohashi , M. , Ashitaka , Y. and Tojo , S. 1983 . Effective extraction and radioimmunoassay of chorionic gonadotropin in human urine . Nippon Sanka Fujinka Gakkai Zasshi , 35 ( 1 ) : 83 – 87 .
   Google Scholar
• Sakakibara , R. , Miyazaki , S. and Ishiguro , M. 1990 . A nicked beta‐subunit of human chorionic gonadotropin purified from pregnancy urine . J. Biochem. (Tokyo) , 107 ( 6 ) : 858 – 862 .
   Google Scholar
• Sakakibara , R. , Miyazaki , S. , Shigemura , T. , Tominaga , N. , Sakai , A. and Ishiguro , M. 1990 . Effective purification of human chorionic gonadotropin and its subunits from pregnant women's urinary peptides absorbed on reverse‐phase resin . Chem. Pharm. Bull. (Tokyo) , 38 ( 5 ) : 1414 – 1416 .
   Google Scholar
• Endo , T. , Nishimura , R. , Saito , S. , Kanazawa , K. , Nomura , K. , Katsuno , M. , Shii , K. , Mukhopadhyay , K. , Baba , S. and Kobata , A. 1992 . Carbohydrate structures of β‐core fragment of Human Chorionic Gonadotropin isolated from a pregnant individual . Endocrinology , 130 ( 4 ) : 2052 – 2058 .
   Google Scholar
• Lowry , O. H. , Rosenborough , N. I. , Farr , A. L. and Randell , R. I. 1951 . Protein measurement with the folin phenol reagent . J. Biol. Chem. , 193 : 265 – 275 .
   PubMed Web of Science ®Google Scholar
• Bradford , M. M. 1976 . A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein‐dye‐binding . Anal. Biochem. , 72 : 248 – 254 .
   PubMed Web of Science ®Google Scholar
• Dubois , M. , Gilles , K. A. , Hamilton , J. K. , Rebers , P. A. and Smith , F. 1956 . Colorimetric method for determination of sugars and related substances . Anal. Chem. , 28 : 350 – 356 .
   Web of Science ®Google Scholar
• Hunter , W. M. and Greenwood , F. C. 1962 . Preparation of iodine‐131 labelled human growth hormone of high specific activity . Nature (London) , 194 : 495 – 496 .
   PubMed Web of Science ®Google Scholar
• Sjodin , L. , Dahlen , H. , Erlandsson‐Persson , K. , Henningsson , A. C. and Viitanen , E. 1990 . Quantitation of human chorionic gonadotropin by radioreceptor assay . J. Pharm. Biomed. Anal. , 8 : 337 – 343 .
   Google Scholar
• Akar , A. H. , Wehmann , R. E. , Blithe , D. L. , Blacker , C. and Nisula , B. C. 1988 . A radioimmunoassay for the core fragment of the human chorionic gonadotropin beta subunit . Endocrinology , 66 : 538 – 545 .
   Web of Science ®Google Scholar
• Prasad Pramod , V. , Chaube Shail , K. , Kumari , G. L. and Shrivastav , T. G. 2005 . Development of colorimetric enzyme‐linked immunosorbent assay for human chorionic gonadotropin . J. Immunoassay Immunochem. , : accepted
   Google Scholar
• Shrivastav , T. G. , Pandey , P. K. and Kumari , G. L. 1988 . Enzyme immunosorbent assay of Prolactin with penicillinase as label . Clin. Chem. , 34 : 2205 – 2207 .
   PubMed Web of Science ®Google Scholar
• Weber , O. 1969 . The reliability of molecular weight determination by dodecyl sulfate‐polyacrylamide gel electrophoresis . J. Biol. Chem. , 244 : 4406 – 4412 .
   PubMed Web of Science ®Google Scholar
• Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature , 227 : 680 – 685 .
   PubMed Web of Science ®Google Scholar
• Davis , B. J. 1964 . Disc electrophoresis. II. Method and application to human serum proteins . Ann. N.Y. Acad. Sci. , 121 : 404 – 427 .
   PubMed Web of Science ®Google Scholar
• Towbin , H. , Staehelin , T. and Gordon , J. 1979 . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications . Proc. Natl. Acad. Sci. USA , 76 : 4350 – 4354 .
   PubMed Web of Science ®Google Scholar
• Warren , L. 1959 . The thiobarbituric acid assay of sialic acids . J. Biol. Chem. , 234 : 1971 – 1975 .
   PubMed Web of Science ®Google Scholar
• Ashitaka , Y. , Mochizuki , M. and Tojo , S. 1972 . Purification and properties of chorionic gonadotropin from the trophoblastic tissue of hydatidiform mole . Endocrinology , 90 ( 3 ) : 609 – 617 .
   Google Scholar
• Swaminathan , N. , Rasor , J. and Braunstein , G. D. 1979 . Preparation and Properties of High titer antisera against the beta subunit of human chorionic gonadotropin . Molec. Immunol. , 16 : 113 – 116 .
   Google Scholar
• Longeon , A. , Henry , J. P. and Henry , R. 1986 . A solid‐phase luminescent immunoassay for human chorionic gonadotropin using Pholas dactylus bioluminescence . J. Immunol. Meth. , 89 : 103 – 109 .
   Google Scholar
• Blithe , D. L. , Akar , A. H. , Wehmann , R. E. and Nisula , B. C. 1988 . Purification of β‐core fragment from pregnancy urine and demonstration that its carbohydrate moieties differ from those of native human chorionic gonadotropin‐β . Endocrinology , 122 ( 1 ) : 173 – 180 .
   Google Scholar
• Lee , C. L. , Iles , R. K. , Shepherd , J. H. , Hudson , C. N. and Chard , T. 1991 . The purification and development of a radioimmunoassay for β‐core fragment of Human Chorionic Gonadotropin in urine: application as a marker of gynaecological cancer in premenopausal and postmenopausal women . J. Endocrinol. , 130 ( 3 ) : 481 – 490 .
   Google Scholar
• Bahl , O. P. 1969 . Human chorionic gonadotropin. I. Purification and physicochemical properties . J. Biol. Chem. , 244 : 567 – 574 .
   PubMed Web of Science ®Google Scholar
• Canfield , R. E. , Morgan , F. J. , Kammermann , S. , Bell , J. J. and Agosto , G. M. 1971 . Studies on human chorionic gonadotropin . Rec. Prog. Horm. Res. , 27 : 121 – 164 .
   PubMedGoogle Scholar
• Qazi , M. H. , Mukherjee , G. , Javidi , K. , Pala , A. and Diczfalusy , E. 1974 . Preparation of highly purified human chorionic gonadotropin by isoelectric focusing . Eur. J. Biochem. , 47 ( 2 ) : 219 – 223 .
   Google Scholar
• Wang , H. , Segal , S. and Koide , S. S. 1988 . Purification and characterization of an incompletely glycosylated form of human chorionic gonadotropin from human placenta . Endocrinology , 123 ( 2 ) : 795 – 803 .
   PubMed Web of Science ®Google Scholar
• Blithe , D. L. and Nisula , B. C. 1985 . Variations in the oligosaccharides on free and combined alpha‐subunits of human choriogonadotropin in pregnancy . Endocrinology , 117 ( 5 ) : 2218 – 2228 .
   Google Scholar
• Deutscher Murray , P. 1990 . “ Characterization of purified proteins ” . In Methods in Enzymology Vol. 182 , 555 – 646 . California : Academic Press Inc. . Guide to Protein Purification
   Google Scholar
• Kapadia , G. , Vaitukaitis , J. L. and Chrambach , A. 1981 . One step isolation of Human Chorionic Gonadotropin in milligram amounts, using selective steady‐state stacking on polyacrylamide gel . Prep. Biochem. , 11 ( 1 ) : 1 – 22 .
   Web of Science ®Google Scholar
• Xu , B. , Zhu , Z. , Tang , Y. , Huang , Q. and Wang , X. 1999 . Purification of human chorionic gonadotropin from Urine by membrane filtration affinity chromatography with a positively charged membrane . Protein Expr. Purif. , 16 ( 2 ) : 221 – 223 .
   Google Scholar
• Bahl , O. P. 1971 . Structure‐activity relationships of protein and polypeptide hormones, Int , Congr. Series No. 241 Edited by: Margoulies , M. and Greenwood , F. C. 99 – 111 . Amsterdam : Excerpta Medica Foundation .
   Google Scholar
• Fein , H. G. , Rosen , S. W. and Weintraub , B. D. 1980 . Increased glycosylation of serum human chorionic gonadotropin and subunits from eutopic and ectopic sources: comparison with placental and urinary forms . J. Clin. Endocrinol. Metab. , 50 : 1111 – 1120 .
   Google Scholar
• Jones‐Brown , Y. R. , Wu , C. Y. , Weintraub , B. D. and Rosen , S. W. 1984 . Synthesis of chorionic gonadotropin subunits in human choriocarcinoma clonal cell line JEG‐3: carbohydrate differences in glycopeptides from free and combined alpha‐subunits . Endocrinology , 115 : 1439 – 1445 .
   Google Scholar
• Puett , D. , Kenner , A. , Benveniste , R. and Rabinowitz , D. 1978 . Characterization of the human chorionic gonadotropin fractions in pregnancy urine . Acta Endocrinol. , 89 : 612 – 624 .
   Google Scholar