Viability and Function in Lymphocytes Cultured from the Horse, Chicken, and Mouse: Effects of Different Leukocyte Enrichment Techniques

REFERENCES

• Klein , A.B. ; Witonsky , S.G. ; Ahmed , S.A. ; Holladay , S.D. ; Gogal , Jr ., R.M. ; Link , L. ; Reilly , C.M. Impact of different cell isolation techniques on lymphocyte viability and function . J Immunoassay Immunochem . 2006 , 27 ( 1 ), 61 – 76 .
   PubMed Web of Science ®Google Scholar
• Ruitenberg , J.J. ; Mulder , C.B. ; Maino , V.C. ; Landay , A.L. ; Ghanekar , S.A. VACUTAINER CPT and Ficoll density gradient separation perform equivalently in maintaining the quality and function of PBMC from HIV seropositive blood samples. BMC Immunol . 2006 , 7 , 11 .
   Google Scholar
• Tochizawa , S. ; Ohmoto , Y. ; Mori , T. A novel modification of a flow cytometric assay of phosphorylated STAT1 in whole blood lymphocytes for rapid detection of interferon-alpha signal in vivo. J Immunol. Meth . 2006 , 313 ( 1 ), 29 – 37 .
   Google Scholar
• Richter , C.A. ; Birnbaum , L.S. ; Farabollini , F. ; Newbold , R.R. ; Rubin , B.S. ; Talsness , C.E. ; Vandenbergh , J.G. ; Walser-Kuntz , D.R. ; Vom Saal , F.S. In vivo effects of bisphenol A in laboratory rodent studies. Reprod. Toxicol . 2007 , 24 ( 2 ): 199 – 224 .
   Google Scholar
• Green , E.L. (.). Biology of the Laboratory Mouse , Ed ; McGraw-Hill : New York , 1966 .
   Google Scholar
• Boyum , A. ; Brincker Fjerdingstad , H. ; Martinsen , I. ; Lea , T. ; Lovhaug , D. Separation of human lymphocytes from citrated blood by density gradient (NycoPrep) centrifugation: monocyte depletion depending upon activation of membrane potassium channels . Scand. J. Immunol . 2002 , 56 ( 1 ), 76 – 84 .
   PubMedGoogle Scholar
• Witonsky , S. ; Gogal , R.M. , Jr.; Buechner-Maxwell , V. ; Ahmed , S.A. Immunologic analysis of blood samples obtained from horses and stored for twenty-four hours . Am. J. Vet. Res . 2003 , 64 ( 8 ), 1003 – 1009 .
   PubMed Web of Science ®Google Scholar
• Ahmed , S.A. ; Gogal , R.M. , Jr.; Walsh , J.E. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: an alternative to [3H]thymidine incorporation assay . J. Immunol. Meth . 1994 , 170 ( 2 ), 211 – 224 .
   PubMed Web of Science ®Google Scholar
• Gogal , Jr ., R.M. ; Ahmed , S.A. ; Larsen , C.T. Analysis of avian lymphocyte proliferation by a new, simple, nonradioactive assay (lympho-pro) . Avian Dis . 1997 , 41 ( 3 ), 714 – 725 .
   PubMed Web of Science ®Google Scholar
• Gogal , Jr ., R.M. ; Prater , M.R. ; Smith , B.J. ; Johnson , M.S. ; Holladay , S.D. Bilateral dissected spleens and thymuses in rodents exhibit homogeneity in leukocyte markers . Toxicology . 2001 , 157 ( 3 ), 217 – 223 .
   PubMed Web of Science ®Google Scholar
• Donner , K.J. ; Becker , K.M. ; Hissong , B.D. ; Ahmed , S.A. Comparison of multiple assays for kinetic detection of apoptosis in thymocytes exposed to dexamethasone or diethylstilbesterol . Cytometry . 1999 , 35 ( 1 ), 80 – 90 .
   PubMedGoogle Scholar
• Gogal , Jr ., R.M. ; Ahmed , S.A. ; Holladay , S.D. ; Walsh , J.E. ; Galaburda , A.M. ; Rosen G.D. Induced minor malformations in the neocortex of normal mice do not alter immunological functions . Immunol. Invest . 2000 , 29 ( 3 ), 299 – 318 .
   PubMed Web of Science ®Google Scholar
• Witonsky , S.G. ; Gogal , Jr ., R.M. ; Duncan , R.B. ; Lindsay , D.S. Protective immune response to experimental infection with Sarcocystis neurona in 57BL/6 mice . J. Parasitol . 2003 , 89 ( 5 ), 924 – 931 .
   PubMed Web of Science ®Google Scholar
• Gogal , Jr ., R.M. ; Smith , B.J. ; Kalnitsky , J. ; Holladay , S.D. Analysis of apoptosis of lymphoid cells in fish exposed to immunotoxic compounds. Cytometry 2000, 39 (4), 310–318.
   PubMedGoogle Scholar
• Bruyninckx , W.J. ; Blancquaert , A.M. Isolation of horse mononuclear cells, especially of monocytes, on Isopaque-Ficoll neutral density gradient . Vet. Immunol. Immunopathol . 1983 , 4 ( 4 ), 493 – 504 .
   PubMedGoogle Scholar
• Moore , T. ; Wilcke , J. ; Chilcoat , C. ; Eyre , P. ; Crisman , M. Functional characterization of equine neutrophils in response to calcium ionophore A23187 and phorbol myristate acetate ex vivo . Vet. Immunol. Immunopathol . 1997 , 56 ( 3–4 ), 233 – 246 .
   PubMedGoogle Scholar
• Altrade;an , S.A. ; Randers , L. ; Rao , G. Comparison of trypan blue dye exclusion and fluorometric assays for mammalian cell viability determinations . Biotechnol. Prog . 1993 , 9 ( 6 ), 671 – 674 .
   PubMedGoogle Scholar
• Schmid , I. ; Uittenbogaart , C.H. ; Keld , B. ; Giorgi , J.V. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry . J. Immunol. Meth . 1994 , 170 ( 2 ), 145 – 157 .
   PubMed Web of Science ®Google Scholar
• Kerr , J.F. ; Wyllie , A.H. ; Currie , A.R. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics . Brit. J. Cancer . 1972 , 26 ( 4 ), 239 – 257 .
   PubMed Web of Science ®Google Scholar