Molecular architecture of the recombinant human MCM2-7 helicase in complex with nucleotides and DNA

References

• Bell SP, Dutta A. DNA replication in eukaryotic cells. Annu Rev Biochem 2002; 71:333-74; PMID:12045100; http://dx.doi.org/10.1146/annurev.biochem.71.110601.135425
   PubMed Web of Science ®Google Scholar
• Mechali M. Eukaryotic DNA replication origins: many choices for appropriate answers. Nat Rev Mol Cell Biol 2010; 11:728-38; PMID:20861881; http://dx.doi.org/10.1038/nrm2976
   PubMed Web of Science ®Google Scholar
• Hills SA, Diffley JF. DNA replication and oncogene-induced replicative stress. Curr Biol 2014; 24:R435-44; PMID:24845676; http://dx.doi.org/10.1016/j.cub.2014.04.012
   PubMed Web of Science ®Google Scholar
• Blow JJ, Gillespie PJ. Replication licensing and cancer–a fatal entanglement? Nat Rev Cancer 2008; 8:799-806; PMID:18756287; http://dx.doi.org/10.1038/nrc2500
   PubMed Web of Science ®Google Scholar
• Frigola J, Remus D, Mehanna A, Diffley JF. ATPase-dependent quality control of DNA replication origin licensing. Nature 2013; 495:339-43; PMID:23474987; http://dx.doi.org/10.1038/nature11920
   PubMed Web of Science ®Google Scholar
• Yeeles JT, Deegan TD, Janska A, Early A, Diffley JF. Regulated eukaryotic DNA replication origin firing with purified proteins. Nature 2015; 519:431-5; PMID:25739503; http://dx.doi.org/10.1038/nature14285
   PubMed Web of Science ®Google Scholar
• Forsburg SL. Eukaryotic MCM proteins: beyond replication initiation. Microbiol Mol Biol Rev 2004; 68:109-31; PMID:15007098; http://dx.doi.org/10.1128/MMBR.68.1.109-131.2004
   PubMed Web of Science ®Google Scholar
• Bochman ML, Schwacha A. The Mcm complex: unwinding the mechanism of a replicative helicase. Microbiol Mol Biol Rev 2009; 73:652-83; PMID:19946136; http://dx.doi.org/10.1128/MMBR.00019-09
   PubMed Web of Science ®Google Scholar
• Evrin C, Clarke P, Zech J, Lurz R, Sun J, Uhle S, Li H, Stillman B, Speck C. A double-hexameric MCM2-7 complex is loaded onto origin DNA during licensing of eukaryotic DNA replication. Proc Natl Acad Sci U S A 2009; 106:20240-5; PMID:19910535; http://dx.doi.org/10.1073/pnas.0911500106
   PubMed Web of Science ®Google Scholar
• Remus D, Beuron F, Tolun G, Griffith JD, Morris EP, Diffley JF. Concerted loading of MCM2-7 double hexamers around DNA during DNA replication origin licensing. Cell 2009; 139:719-30; PMID:19896182; http://dx.doi.org/10.1016/j.cell.2009.10.015
   PubMed Web of Science ®Google Scholar
• Li N, Zhai Y, Zhang Y, Li W, Yang M, Lei J, Tye BK, Gao N. Structure of the eukaryotic MCM complex at 3.8 A. Nature 2015; 524:186-91; PMID:26222030
   PubMed Web of Science ®Google Scholar
• Labib K. How do Cdc7 and cyclin-dependent kinases trigger the initiation of chromosome replication in eukaryotic cells? Genes Dev 2010; 24:1208-19; PMID:20551170; http://dx.doi.org/10.1101/gad.1933010
   PubMed Web of Science ®Google Scholar
• Tanaka S, Araki H. Helicase activation and establishment of replication forks at chromosomal origins of replication. Cold Spring Harb Perspect Biol 2013; 5:a010371; PMID:23881938; http://dx.doi.org/10.1101/cshperspect.a010371
   PubMed Web of Science ®Google Scholar
• Heller RC, Kang S, Lam WM, Chen S, Chan CS, Bell SP. Eukaryotic origin-dependent DNA replication in vitro reveals sequential action of DDK and S-CDK kinases. Cell 2011; 146:80-91; PMID:21729781; http://dx.doi.org/10.1016/j.cell.2011.06.012
   PubMed Web of Science ®Google Scholar
• Costa A, Ilves I, Tamberg N, Petojevic T, Nogales E, Botchan MR, Berger JM. The structural basis for MCM2-7 helicase activation by GINS and Cdc45. Nat Struct Mol Biol 2011; 18:471-7; PMID:21378962; http://dx.doi.org/10.1038/nsmb.2004
   PubMed Web of Science ®Google Scholar
• Bochman ML, Schwacha A. The MCM2-7 complex has in vitro helicase activity. Mol Cell 2008; 31:287-93; PMID:18657510; http://dx.doi.org/10.1016/j.molcel.2008.05.020
   PubMed Web of Science ®Google Scholar
• Lyubimov AY, Costa A, Bleichert F, Botchan MR, Berger JM. ATP-dependent conformational dynamics underlie the functional asymmetry of the replicative helicase from a minimalist eukaryote. Proc Natl Acad Sci U S A 2012; 109:11999-2004; PMID:22778422; http://dx.doi.org/10.1073/pnas.1209406109
   PubMed Web of Science ®Google Scholar
• Hesketh EL, Parker-Manuel RP, Chaban Y, Satti R, Coverley D, Orlova EV, Chong JP. DNA induces conformational changes in a recombinant human minichromosome maintenance complex. J Biol Chem 2015; 290:7973-9; PMID:25648893; http://dx.doi.org/10.1074/jbc.M114.622738
   PubMed Web of Science ®Google Scholar
• Samel SA, Fernandez-Cid A, Sun J, Riera A, Tognetti S, Herrera MC, Li H, Speck C. A unique DNA entry gate serves for regulated loading of the eukaryotic replicative helicase MCM2-7 onto DNA. Genes Dev 28:1653-66; PMID:25085418; http://dx.doi.org/10.1101/gad.242404.114
   PubMed Web of Science ®Google Scholar
• Coster G, Frigola J, Beuron F, Morris EP, Diffley JF. Origin licensing requires ATP binding and hydrolysis by the MCM replicative helicase. Mol Cell 2014; 55:666-77; PMID:25087873; http://dx.doi.org/10.1016/j.molcel.2014.06.034
   PubMed Web of Science ®Google Scholar
• Fitzgerald DJ, Berger P, Schaffitzel C, Yamada K, Richmond TJ, Berger I. Protein complex expression by using multigene baculoviral vectors. Nat Methods 2006; 3:1021-32; PMID:17117155; http://dx.doi.org/10.1038/nmeth983
   PubMed Web of Science ®Google Scholar
• Bochman ML, Schwacha A. Differences in the single-stranded DNA binding activities of MCM2-7 and MCM467: MCM2 and MCM5 define a slow ATP-dependent step. J Biol Chem 2007; 282:33795-804; PMID:17895243; http://dx.doi.org/10.1074/jbc.M703824200
   PubMed Web of Science ®Google Scholar
• Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, Ludtke SJ. EMAN2: an extensible image processing suite for electron microscopy. J Struct Biol 2007; 157:38-46; PMID:16859925; http://dx.doi.org/10.1016/j.jsb.2006.05.009
   PubMed Web of Science ®Google Scholar
• Costa A, Renault L, Swuec P, Petojevic T, Pesavento JJ, Ilves I, MacLellan-Gibson K, Fleck RA, Botchan MR, Berger JM. DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome. Elife 2014; 3:e03273; PMID:25117490
   PubMed Web of Science ®Google Scholar
• Sanchez-Berrondo J, Mesa P, Ibarra A, Martinez-Jimenez MI, Blanco L, Mendez J, Boskovic J, Montoya G. Molecular architecture of a multifunctional MCM complex. Nucleic Acids Res 2012; 40:1366-80; PMID:21984415; http://dx.doi.org/10.1093/nar/gkr831
   PubMed Web of Science ®Google Scholar
• Froelich CA, Kang S, Epling LB, Bell SP, Enemark EJ. A conserved MCM single-stranded DNA binding element is essential for replication initiation. Elife 2014; 3:e01993; PMID:24692448; http://dx.doi.org/10.7554/eLife.01993
   PubMed Web of Science ®Google Scholar
• Moyer SE, Lewis PW, Botchan MR. Isolation of the Cdc45/MCM2-7/GINS (CMG) complex, a candidate for the eukaryotic DNA replication fork helicase. Proc Natl Acad Sci U S A 2006; 103:10236-41; PMID:16798881; http://dx.doi.org/10.1073/pnas.0602400103
   PubMed Web of Science ®Google Scholar
• Ilves I, Petojevic T, Pesavento JJ, Botchan MR. Activation of the MCM2-7 helicase by association with Cdc45 and GINS proteins. Mol Cell 2010; 37:247-58; PMID:20122406; http://dx.doi.org/10.1016/j.molcel.2009.12.030
   PubMed Web of Science ®Google Scholar
• Bieniossek C, Richmond TJ, Berger I. MultiBac: multigene baculovirus-based eukaryotic protein complex production. Curr Protoc Protein Sci 2008; 51:5.20.1-5.20.26; PMID:18429060
   PubMedGoogle Scholar
• Mindell JA, Grigorieff N. Accurate determination of local defocus and specimen tilt in electron microscopy. J Struct Biol 2003; 142:334-47; PMID:12781660; http://dx.doi.org/10.1016/S1047-8477(03)00069-8
   PubMed Web of Science ®Google Scholar
• Scheres SH, Nunez-Ramirez R, Sorzano CO, Carazo JM, Marabini R. Image processing for electron microscopy single-particle analysis using XMIPP. Nat Protoc 2008; 3:977-90; PMID:18536645; http://dx.doi.org/10.1038/nprot.2008.62
   PubMed Web of Science ®Google Scholar
• Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE. UCSF Chimera–a visualization system for exploratory research and analysis. J Comput Chem 2004; 25:1605-12; PMID:15264254; http://dx.doi.org/10.1002/jcc.20084
   PubMed Web of Science ®Google Scholar