Deoxynivalenol globally affects the selection of 3’ splice sites in human cells by suppressing the splicing factors, U2AF1 and SF1

References

• Sharp PA. The discovery of split genes and RNA splicing: trends in biochemical sciences. Trends Biochem Sci. 2005;30:279–281.
   PubMed Web of Science ®Google Scholar
• Sharp PA. Split genes and RNA splicing (Nobel Lecture). Angew Chem. 2010;33:1229–1240.
   Google Scholar
• Kim E, Magen A, Ast G. Different levels of alternative splicing among eukaryotes. Nucleic Acids Res. 2007;35:125–131.
   PubMed Web of Science ®Google Scholar
• Merkin J, Russell C, Chen P, et al. Evolutionary dynamics of gene and isoform regulation in mammalian tissues. Science. 2012;338:1593–1599.
   PubMed Web of Science ®Google Scholar
• Barbosamorais NL, Irimia M, Pan Q, et al. The evolutionary landscape of alternative splicing in vertebrate species. Science. 2013;338:1587–1593.
   Web of Science ®Google Scholar
• Nilsen TW, Graveley BR. Expansion of the eukaryotic proteome by alternative splicing. Nature. 2010;463:457–463.
   PubMed Web of Science ®Google Scholar
• Irimia M, Blencowe BJ. Alternative splicing: decoding an expansive regulatory layer. Curr Opin Cell Biol. 2012;24:323–332.
   PubMed Web of Science ®Google Scholar
• Modrek B, Resch A, Grasso C, et al. Genome-wide detection of alternative splicing in expressed sequences of human genes. Nucleic Acids Res. 2001;29:2850–2859.
   Google Scholar
• Garcia-Blanco MA, Baraniak AP, Lasda EL. Alternative splicing in disease and therapy. Nat Biotechnol. 2004;22:535–546.
   PubMed Web of Science ®Google Scholar
• Aoki-Suzuki M, Yamada K, Meerabux J, et al. A family-based association study and gene expression analyses of netrin-G1 and -G2 genes in schizophrenia. Biol Psychiatry. 2005;57:382–393.
   PubMed Web of Science ®Google Scholar
• Newman A. Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre‐mRNAs into spliceosomes. Embo J. 1987;6:3833–3839.
   PubMed Web of Science ®Google Scholar
• Ma L, Tan Z, Teng Y, et al. In vivo effects on intron retention and exon skipping by the U2AF large subunit and SF1/BBP in the nematode Caenorhabditis elegans. RNA. 2011;17:2201–2211.
   PubMed Web of Science ®Google Scholar
• Wahl MC, Will CL, Lührmann R. The spliceosome: design principles of a dynamic RNP machine. Cell. 2009;136:701–718.
   PubMed Web of Science ®Google Scholar
• Berglund JA, Chua K, Abovich N, et al. The splicing factor BBP interacts specifically with the pre-mRNA branchpoint sequence UACUAAC. Cell. 1997;89:781.
   PubMed Web of Science ®Google Scholar
• Berglund JA, Abovich N, Rosbash M. A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition. Genes Dev. 1998;12:858–867.
   PubMed Web of Science ®Google Scholar
• Angela C, Flore R, Ivona B, et al. Mammalian splicing factor SF1 interacts with SURP domains of U2 snRNP-associated proteins. Nucleic Acids Res. 2015;43:10456–10473.
   PubMed Web of Science ®Google Scholar
• Moore MJ. Intron recognition comes of AGe. Nat Struct Biol. 2000;7:14–16.
   PubMedGoogle Scholar
• Schothorst RC, Egmond HPV. Report from SCOOP task 3.2.10“collection of occurrence data of Fusarium toxins in food and assessment of dietary intake by the population of EU member states”: subtask: trichothecenes. Toxicol Lett. 2004;153:133–143.
   PubMed Web of Science ®Google Scholar
• Pestka JJ, Smolinski AT. Deoxynivalenol: toxicology and Potential Effects on Humans. J Toxicol Environ Health B Crit Rev. 2005;8:39–69.
   PubMed Web of Science ®Google Scholar
• Knutsen HK, Alexander J, Barregård L, et al. Risks to human and animal health related to the presence of deoxynivalenol and its acetylated and modified forms in food and feed. Efsa J. 2017;15:4718.
   Web of Science ®Google Scholar
• Rotter BA, Prelusky DB, Pestka JJ. Toxicology of deoxynivalenol (vomitoxin). J Toxicol Environ Health. 1996;48:1–34.
   PubMed Web of Science ®Google Scholar
• Wells L, Hardie L, Williams C, et al. Deoxynivalenol Biomarkers in the Urine of UK Vegetarians. Toxins (Basel). 2017;9:196.
   Web of Science ®Google Scholar
• Berthiller F, Dall’Asta C, Schuhmacher R, et al. Masked mycotoxins: determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography−tandem mass spectrometry. J Agric Food Chem. 2005;53:3421–3425.
   PubMed Web of Science ®Google Scholar
• Listed N. Evaluation of certain mycotoxins in food. Fifty-sixth report of the Joint FAO/WHO Expert Committee on Food Additives. World Health Organ Tech Rep. 2002;906:1–62.
   PubMedGoogle Scholar
• Bhat R, Ramakrishna Y, Beedu S, et al. Outbreak of trichothecene mycotoxicosis associated with consumption of mould-damaged wheat products in Kashmir Valley, India. Lancet. 1989;333:35–37.
   Google Scholar
• Mishra S, Dwivedi PD, Pandey HP, et al. Role of oxidative stress in Deoxynivalenol induced toxicity. Food Chem Toxicol. 2014;72:20–29.
   PubMed Web of Science ®Google Scholar
• Friend DW, Thompson BK, Trenholm HL, et al. Effects of feeding deoxynivalenol (DON)-contaminated wheat diets to pregnant and lactating gilts and on their progeny. Can Vet J. 1986;66:229–236.
   Google Scholar
• Dänicke S, Valenta H, Spilke J. Effects of long-term storage on Fusarium toxin concentrations in wheat - sources of error of the analytical results. Arch Tierernahr. 2004;58:507–515.
   Google Scholar
• Pinton P, Tsybulskyy D, Lucioli J, et al. Toxicity of deoxynivalenol and its acetylated derivatives on the intestine: differential effects on morphology, barrier function, tight junction proteins, and mitogen-activated protein kinases. Toxicol Sci. 2012;130:180–190.
   PubMed Web of Science ®Google Scholar
• Juan-García A, Juan C, König S, et al. Cytotoxic effects and degradation products of three mycotoxins: alternariol, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol in liver hepatocellular carcinoma cells. Toxicol Lett. 2015;235:8–16.
   PubMed Web of Science ®Google Scholar
• Shifrin VI, Anderson P. Trichothecene mycotoxins trigger a ribotoxic stress response that activates c-Jun N-terminal kinase and p38 mitogen-activated protein kinase and induces apoptosis. J Biol Chem. 1999;274:13985–13992.
   PubMed Web of Science ®Google Scholar
• Pestka JJ. Deoxynivalenol-Induced Proinflammatory Gene Expression: mechanisms and Pathological Sequelae. Toxins (Basel). 2010;2:1300–1317.
   PubMed Web of Science ®Google Scholar
• Zhu J, Krainer AR. Pre-mRNA splicing in the absence of an SR protein RS domain. Genes Dev. 2000;14:3166–3178.
   PubMed Web of Science ®Google Scholar
• Nilsen TW. The spliceosome: the most complex macromolecular machine in the cell? Bioessays. 2003;25:1147–1149.
   PubMed Web of Science ®Google Scholar
• Yuan L, Mu P, Huang B, et al. EGR1 is essential for deoxynivalenol-induced G2/M cell cycle arrest in HepG2 cells via the ATF3ΔZip2a/2b-EGR1-p21 pathway. Toxicol Lett. 2018;299:95–103.
   PubMed Web of Science ®Google Scholar
• Xiaoming L, Peiqiang M, Han Q, et al. JNK-AKT-NF-κB controls P-glycoprotein expression to attenuate the cytotoxicity of Deoxynivalenol in mammalian cells. Biochem Pharmacol. 2018;156:120–134.
   Web of Science ®Google Scholar
• Krämer A, Utans U. Three protein factors (SF1, SF3 and U2AF) function in pre‐splicing complex formation in addition to snRNPs. The EMBO Journal. 2010;2:1300–1317.
   Google Scholar
• Nielsen C, Casteel M, Didier A, et al. Trichothecene-induced cytotoxicity on human cell lines. Mycotoxin Res. 2009;25:77–84.
   PubMedGoogle Scholar
• Kanaar R, Roche SE, Beall EL, et al. The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability. Science. 1993;262:569–573.
   PubMed Web of Science ®Google Scholar
• Golling G, Amsterdam A, Sun Z, et al. Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development. Nat Genet. 2002;31:135–140.
   PubMed Web of Science ®Google Scholar
• Dvinge H, Kim E, Abdelwahab O, et al. RNA splicing factors as oncoproteins and tumour suppressors. Nat Rev Cancer. 2016;16:413–430.
   Web of Science ®Google Scholar
• Yoshida K, Sanada M, Shiraishi Y, et al. Frequent pathway mutations of splicing machinery in myelodysplasia. Nature. 2011;478:64–69.
   Web of Science ®Google Scholar
• Yip BH, Steeples V, Repapi E, et al. The U2AF1 S34F mutation induces lineage-specific splicing alterations in myelodysplastic syndromes. J Clin Investig. 2017;127:2206–2221.
   PubMed Web of Science ®Google Scholar
• Wang L, Brooks AN, Fan J, et al. Transcriptomic Characterization of SF3B1 Mutation Reveals Its Pleiotropic Effects in Chronic Lymphocytic Leukemia. Cancer Cell. 2016;30:750–763.
   PubMed Web of Science ®Google Scholar
• Graubert TA, Shen D, Ding L, et al. Recurrent mutations in the U2AF1 splicing factor in myelodysplastic syndromes. Nat Genet. 2012;44:53–57.
   Web of Science ®Google Scholar
• Black DL, Grabowski PJ. Alternative pre-mRNA splicing and neuronal function. Prog Mol Subcell Biol. 2003;31:187–216.
   PubMedGoogle Scholar
• Sun Y, Wen J, Chen R, et al. Variable protein homeostasis in housekeeping and non-housekeeping pathways under mycotoxins stress. Sci Rep. 2019;9:7819.
   PubMed Web of Science ®Google Scholar
• Reed R. Protein composition of mammalian spliceosomes assembled in vitro. Proc Natl Acad Sci U S A. 1990;87:8031–8035.
   PubMed Web of Science ®Google Scholar
• Michaud S, Reed R. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway. Genes Dev. 1991;5:2534–2546.
   Web of Science ®Google Scholar
• Hinnebusch AG. Translational control of GCN4: an in vivo barometer of initiation-factor activity. Trends Biochem Sci. 1994;19:409–414.
   PubMed Web of Science ®Google Scholar
• Shufeng S, Jun J, Yiqun D. Chicken cytochrome P450 1A5 is the key enzyme for metabolizing T-2 toxin to 3ʹOH-T-2. Int J Mol Sci. 2013;14:10809–10818.
   PubMed Web of Science ®Google Scholar
• Chatrikhi R, Wang W, Gupta A, et al. SF1 Phosphorylation Enhances Specific Binding to U2AF65 and Reduces Binding to 3â2-Splice-Site RNA. Biophys J. 2016;111:2570–2586.
   PubMed Web of Science ®Google Scholar
• Komeno Y, Huang YJ, Qiu J, et al. SRSF2 Is Essential for Hematopoiesis, and Its Myelodysplastic Syndrome-Related Mutations Dysregulate Alternative Pre-mRNA Splicing. Mol Cell Biol. 2015;35:3071–3082.
   PubMed Web of Science ®Google Scholar
• Dvinge H, Bertone P. HTqPCR: high-throughput analysis and visualization of quantitative real-time PCR data in R. Bioinformatics. 2009;25:3325–3326.
   PubMed Web of Science ®Google Scholar
• Hurgobin B. Short Read Alignment Using SOAP2. Methods Mol Biol. 2016;1374:241–252.
   Google Scholar