Inhibition of WNT-CTNNB1 signaling upregulates SQSTM1 and sensitizes glioblastoma cells to autophagy blockers

References

• Louis DN, Ohgaki H, Wiestler OD, et al. The 2007 WHO classification of tumours of the central nervous system. Acta Neuropathol. 2007;114(2):97–109. doi:10.1007/s00401-007-0243-4. PMID:17618441
   PubMed Web of Science ®Google Scholar
• Nager M, Bhardwaj D, Cantí C, et al. β-Catenin signalling in glioblastoma multiforme and glioma-initiating cells. Chemother Res Pract. 2012;2012:192362. PMID:22400111
   PubMedGoogle Scholar
• Furnari FB, Fenton T, Bachoo RM, et al. Malignant astrocytic glioma: genetics, biology, and paths to treatment. Genes Dev. 2007;21(21):2683–2710. doi:10.1101/gad.1596707. PMID:17974913
   PubMed Web of Science ®Google Scholar
• Kleihues P, Louis DN, Scheithauer BW, et al. The WHO classification of tumors of the nervous system. J Neuropathol Exp Neurol. 2002;61(3):215–225. doi:10.1093/jnen/61.3.215.
   PubMed Web of Science ®Google Scholar
• Morokoff A, Ng W, Gogos A, et al. Molecular subtypes, stem cells and heterogeneity: implications for personalised therapy in glioma. J Clin Neurosci. 2015;22(8):1219–1226. doi:10.1016/j.jocn.2015.02.008. PMID:25957782
   PubMed Web of Science ®Google Scholar
• Klaus A, Birchmeier W. Wnt signalling and its impact on development and cancer. Nat Rev Cancer. 2008;8:387–398. doi:10.1038/nrc2389. PMID:18432252
   PubMed Web of Science ®Google Scholar
• Barker N, Clevers H. Mining the Wnt pathway for cancer therapeutics. Nat Rev Drug Discov. 2006;5(12):997–1014. doi:10.1038/nrd2154. PMID:17139285
   PubMed Web of Science ®Google Scholar
• Clevers H, Nusse R. Wnt/β-catenin signaling and disease. Cell. 2012;149(6):1192–1205. doi:10.1016/j.cell.2012.05.012. PMID:22682243
   PubMed Web of Science ®Google Scholar
• Kaur N, Chettiar S, Rathod S, et al. Wnt3a mediated activation of Wnt/beta-catenin signaling promotes tumor progression in glioblastoma. Mol Cell Neurosci. 2013;54:44–57. doi:10.1016/j.mcn.2013.01.001. PMID:23337036
   PubMed Web of Science ®Google Scholar
• Zhang N, Wei P, Gong A, et al. FoxM1 promotes β-Catenin nuclear localization and controls Wnt target-gene expression and glioma tumorigenesis. Cancer Cell. 2011;20(4):427–442. doi:10.1016/j.ccr.2011.08.016. PMID:22014570
   PubMed Web of Science ®Google Scholar
• Zheng H, Ying H, Wiedemeyer R, et al. PLAGL2 regulates Wnt signaling to impede differentiation in neural stem cells and gliomas. Cancer Cell. 2010;17(5):497–509. doi:10.1016/j.ccr.2010.03.020. PMID:20478531
   PubMed Web of Science ®Google Scholar
• Pulvirenti T, Van Der Heijden M, Droms LA, et al. Dishevelled 2 signaling promotes self-renewal and tumorigenicity in human gliomas. Cancer Res. 2011;71(23):7280–7290. doi:10.1158/0008-5472.CAN-11-1531. PMID:21990322
   PubMed Web of Science ®Google Scholar
• Náger M, Santacana M, Bhardwaj D, et al. Nuclear phosphorylated Y142 β-catenin accumulates in astrocytomas and glioblastomas and regulates cell invasion. Cell Cycle. 2015;14(22):3644–3655. doi:10.1080/15384101.2015.1104443. PMID:26654598
   PubMed Web of Science ®Google Scholar
• Kaur J, Debnath J. Autophagy at the crossroads of catabolism and anabolism. Nat Rev Mol Cell Biol. 2015;16(8):461–472. doi:10.1038/nrm4024. PMID:26177004
   PubMed Web of Science ®Google Scholar
• Galluzzi L, Pietrocola F, Bravo-San Pedro JM, et al, Autophagy in malignant transformation and cancer progression. EMBO J. 2015;34(7):856–880. doi:10.15252/embj.201490784. PMID:25712477
   PubMed Web of Science ®Google Scholar
• Laplante M, Sabatini DM. mTOR signaling at a glance. J Cell Sci. 2009;122(Pt 20):3589–3594. doi:10.1242/jcs.051011. PMID:19812304
   PubMed Web of Science ®Google Scholar
• Gao C, Cao W, Bao L, et al. Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation. Nat Cell Biol. 2010;12(8):781–790. doi:10.1038/ncb2082. PMID:20639871
   PubMed Web of Science ®Google Scholar
• Petherick KJ, Williams AC, Lane JD, et al. Autolysosomal β-catenin degradation regulates Wnt-autophagy-p62 crosstalk. EMBO J. 2013;32(13):1903–1916. doi:10.1038/emboj.2013.123. PMID:23736261
   PubMed Web of Science ®Google Scholar
• Zhang Y, Wang F, Han L, et al. GABARAPL1 negatively regulates Wnt/β-catenin signaling by mediating Dvl2 degradation through the autophagy pathway. Cell Physiol Biochem. 2011;27(5):503–512. doi:10.1159/000329952. PMID:21691068
   PubMed Web of Science ®Google Scholar
• Pankiv S, Clausen TH, Lamark T, et al. p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J Biol Chem. 2007;282(33):24131–24145. doi:10.1074/jbc.M702824200. PMID:17580304
   PubMed Web of Science ®Google Scholar
• Inoki K, Ouyang H, Zhu T, et al. TSC2 integrates Wnt and energy signals via a coordinated phosphorylation by AMPK and GSK3 to regulate cell growth. Cell. 2006;126(5):955–968. doi:10.1016/j.cell.2006.06.055. PMID:16959574
   PubMed Web of Science ®Google Scholar
• Settembre C, Di Malta C, Polito VA, et al. TFEB links autophagy to lysosomal biogenesis. Science. 2011;332(6036):1429–1433. doi:10.1126/science.1204592. PMID:21617040
   PubMed Web of Science ®Google Scholar
• Sardiello M, Palmieri M, di Ronza A, et al. A gene network regulating lysosomal biogenesis and function. Science. 2009;325:473–477. PMID:19556463
   PubMed Web of Science ®Google Scholar
• Bowman R, Wang Q, Carro A, et al. GlioVis data portal for visualization and analysis of brain tumor expression datasets. Neuro Oncol. 2016;19(1):139–141. doi:10.1093/neuonc/now247. PMID:28031383
   PubMed Web of Science ®Google Scholar
• Handeli S, Simon JA. A small-molecule inhibitor of Tcf/β -catenin signaling down-regulates PPARγ and PPARδ activities. Mol Cancer Ther. 2008;7(3):521–529. doi:10.1158/1535-7163.MCT-07-2063. PMID:18347139
   PubMed Web of Science ®Google Scholar
• Klionsky DJ, Abdelmohsen K, Abe A, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 2016;12(1):1–122. doi:10.1080/15548627.2015.1100356. PMID:26799652
   PubMed Web of Science ®Google Scholar
• Kimura S, Noda T, Yoshimori T. Dissection of the Autophagosome maturation process by a novel reporter protein, tandem fluorescent tagged LC3. Autophagy. 2007;3(5):452–460. doi:10.4161/auto.4451. PMID:17534139
   PubMed Web of Science ®Google Scholar
• Laplante M, Sabatini DM. Regulation of mTORC1 and its impact on gene expression at a glance. J Cell Sci. 2013;126(Pt 8):1713–1719. doi:10.1242/jcs.125773. PMID:23641065
   PubMed Web of Science ®Google Scholar
• Saxton RA, Sabatiniti DM. mTOR signaling in growth, metabolism, and disease. Cell. 2017;168(6):960–976. doi:10.1016/j.cell.2017.02.004. PMID:28283069
   PubMed Web of Science ®Google Scholar
• Settembre C, Fraldi A, Medina DL, et al. Signals from the lysosome: a control centre for cellular clearance and energy metabolism. Nat Rev Mol Cell Biol. 2013;14(5):283–296. doi:10.1038/nrm3565. PMID:23609508
   PubMed Web of Science ®Google Scholar
• Martina JA, Chen Y, Gucek M, et al. MTORC1 functions as a transcriptional regulator of autophagy by preventing nuclear transport of TFEB. Autophagy. 2012;8(6):903–914. doi:10.4161/auto.19653. PMID:22576015
   PubMed Web of Science ®Google Scholar
• Roczniak-Ferguson A, Petit CS, Froehlich F, et al. The transcription factor TFEB links mTORC1 signaling to transcriptional control of lysosome homeostasis. Sci Signal. 2012;5(228):ra42. doi:10.1126/scisignal.2002790. PMID:22692423
   PubMed Web of Science ®Google Scholar
• Niehrs C, Acebron SP. Mitotic and mitogenic Wnt signalling. EMBO J. 2012;31(12):2705–2713. doi:10.1038/emboj.2012.124. PMID:22617425
   PubMed Web of Science ®Google Scholar
• Lamouille S, Xu J, Derynck R. Molecular mechanisms of epithelial–mesenchymal transition. Nat Rev Mol Cell Biol. 2014;15(3):178–196. doi:10.1038/nrm3758. PMID:24556840
   PubMed Web of Science ®Google Scholar
• Huang S, Okamoto K, Yu C, et al. P62/sequestosome-1 Up-regulation promotes ABT-263-induced caspase-8 aggregation/activation on the autophagosome. J Biol Chem. 2013;288(47):33654–33666. doi:10.1074/jbc.M113.518134. PMID:24121507
   PubMed Web of Science ®Google Scholar
• Young MM, Takahashi Y, Khan O, et al. Autophagosomal membrane serves as platform for intracellular death-inducing signaling complex (iDISC)-mediated caspase-8 activation and apoptosis. J Biol Chem. 2012;287(15):12455–12468. doi:10.1074/jbc.M111.309104. PMID:22362782
   PubMed Web of Science ®Google Scholar
• Dihlmann S, Von Knebel Doeberitz M. Wnt/β-catenin-pathway as a molecular target for future anti-cancer therapeutics. Int J Cancer. 2005;113(4):515–524. doi:10.1002/ijc.20609. PMID:15472907
   PubMed Web of Science ®Google Scholar
• Bjørkøy G, Lamark T, Brech A, et al. p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death. J Cell Biol. 2005;171(4):603–614. doi:10.1083/jcb.200507002. PMID:16286508
   PubMed Web of Science ®Google Scholar
• Jiang P, Mizushima N. LC3- and p62-based biochemical methods for the analysis of autophagy progression in mammalian cells. Methods. 2015;75:13–18. doi:10.1016/j.ymeth.2014.11.021. PMID:25484342
   PubMed Web of Science ®Google Scholar
• Puissant A, Fenouille N, Auberger P. When autophagy meets cancer through p62/SQSTM1. Am J Cancer Res. 2012;2(4):397–413. PMID:22860231
   PubMed Web of Science ®Google Scholar
• Moscat J, Diaz-Meco MT. p62 at the crossroads of autophagy, apoptosis, and cancer. Cell. 2009;137(6):1001–1004. doi:10.1016/j.cell.2009.05.023. PMID:19524504
   PubMed Web of Science ®Google Scholar
• Jennewein L, Ronellenfitsch MW, Antonietti P, et al Diagnostic and clinical relevance of the autophago-lysosomal network in human gliomas. Oncotarget. 2016;7(15):20016–20032. doi:10.18632/oncotarget.7910. PMID:26956048
   PubMedGoogle Scholar
• Ma B, Liu B, Cao W, et al. The Wnt signaling antagonist dapper1 accelerates dishevelled2 degradation via promoting its ubiquitination and aggregate-induced autophagy. J Biol Chem. 2015;290(19):12346–12354. doi:10.1074/jbc.M115.654590. PMID:25825496
   PubMed Web of Science ®Google Scholar
• Chang HW, Lee YS, Nam HY, et al. Knockdown of β-catenin controls both apoptotic and autophagic cell death through LKB1/AMPK signaling in head and neck squamous cell carcinoma cell lines. Cell Signal. 2013;25(4):839–847. doi:10.1016/j.cellsig.2012.12.020. PMID:23280187
   PubMed Web of Science ®Google Scholar
• Kraya AA, Piao S, Xu X, et al. Identification of secreted proteins that reflect autophagy dynamics within tumor cells. Autophagy. 2015;11(1):60–74. doi:10.4161/15548627.2014.984273. PMID:25484078
   PubMed Web of Science ®Google Scholar
• Napolitano G, Ballabio A. TFEB at a glance. J Cell Sci. 2016;129:2475–2481. doi:10.1242/jcs.146365. PMID:27252382
   PubMed Web of Science ®Google Scholar
• Settembre C, Zoncu R, Medina DL, et al. A lysosome-to-nucleus signalling mechanism senses and regulates the lysosome via mTOR and TFEB. EMBO J. 2012;31(5):1095–1108. doi:10.1038/emboj.2012.32. PMID:22343943
   PubMed Web of Science ®Google Scholar
• Shi Z, Qian X, Li L, et al. Nuclear translocation of β-catenin is essential for glioma cell survival. J Neuroimm Pharmacol. 2012;7(4):892–903. doi:10.1007/s11481-012-9354-3.
   PubMed Web of Science ®Google Scholar
• Lee SW, Kim HK, Lee NH, et al. The synergistic effect of combination temozolomide and chloroquine treatment is dependent on autophagy formation and p53 status in glioma cells. Cancer Lett. 2015;360(2):195–204. doi:10.1016/j.canlet.2015.02.012. PMID:25681668
   PubMed Web of Science ®Google Scholar
• Kanzawa T, Germano IM, Komata T, et al. Role of autophagy in temozolomide-induced cytotoxicity for malignant glioma cells. Cell Death Differ. 2004;11(4):448–457. doi:10.1038/sj.cdd.4401359. PMID:14713959
   PubMed Web of Science ®Google Scholar
• Golden EB, Cho H-Y, Jahanian A, et al. Chloroquine enhances temozolomide cytotoxicity in malignant gliomas by blocking autophagy. Neurosurg Focus. 2014;37(6):E12. doi:10.3171/2014.9.FOCUS14504. PMID:25434381
   PubMed Web of Science ®Google Scholar
• Llobet D, Eritja N, Encinas M, et al. CK2 controls TRAIL and Fas sensitivity by regulating FLIP levels in endometrial carcinoma cells. Oncogene. 2008;27(18):2513–2524. doi:10.1038/sj.onc.1210924. PMID:17982483
   PubMed Web of Science ®Google Scholar
• Pallares J, Martínez-Guitarte JL, Dolcet X, et al. Abnormalities in the NF-κB family and related proteins in endometrial carcinoma. J Pathol. 2004;204(5):569–577. doi:10.1002/path.1666. PMID:15481028
   PubMed Web of Science ®Google Scholar