GPX8 deficiency–induced oxidative stress reprogrammed m6A epitranscriptome of oral cancer cells

References

• Buday K, Conrad M. Emerging roles for non-selenium containing ER-resident glutathione peroxidases in cell signaling and disease. Biol Chem. 2021;402(3):271–15.
   PubMed Web of Science ®Google Scholar
• Ramming T, Hansen HG, Nagata K, et al. Gpx8 peroxidase prevents leakage of H2O2 from the endoplasmic reticulum. Free Radical Bio Med. 2014;70:106–116.
   PubMed Web of Science ®Google Scholar
• Morikawa K, Gouttenoire J, Hernandez C, et al. Quantitative proteomics identifies the membrane-associated peroxidase GPx8 as a cellular substrate of the hepatitis C virus NS3-4A protease. Hepatology. 2014;59(2):423–433. DOI:10.1002/hep.26671
   PubMed Web of Science ®Google Scholar
• Yoboue ED, Rimessi A, Anelli T, et al. Regulation of calcium fluxes by GPX8, a Type-II transmembrane peroxidase enriched at the mitochondria-associated endoplasmic reticulum membrane. Antioxid Redox Signal. 2017;27(9):583–595. DOI:10.1089/ars.2016.6866
   PubMed Web of Science ®Google Scholar
• Mehmeti I, Lortz S, Avezov E, et al. ER-resident antioxidative GPx7 and GPx8 enzyme isoforms protect insulin-secreting INS-1E beta-cells against lipotoxicity by improving the ER antioxidative capacity. Free Radic Biol Med. 2017;112:121–130.
   PubMed Web of Science ®Google Scholar
• Hsu JL, Chou JW, Chen TF, et al. Glutathione peroxidase 8 negatively regulates caspase-4/11 to protect against colitis. EMBO Mol Med. 2020;12(1):e9386. DOI:10.15252/emmm.201809386
   PubMed Web of Science ®Google Scholar
• Bosello Travain V, Miotto G, Vuckovic AM, et al. Lack of glutathione peroxidase-8 in the ER impacts on lipid composition of HeLa cells microsomal membranes. Free Radic Biol Med. 2020;147:80–89.
   PubMed Web of Science ®Google Scholar
• Zhou Y, Zhang D, Liu B, et al. Bioinformatic identification of key genes and molecular pathways in the spermatogenic process of cryptorchidism. Genes Dis. 2019;6(4):431–440. DOI:10.1016/j.gendis.2018.11.002
   PubMed Web of Science ®Google Scholar
• Wu JH, Wang X, Wang N, et al. Identification of novel antioxidant gene signature to predict the prognosis of patients with gastric cancer. World J Surg Oncol. 2021;19(1):219. DOI:10.1186/s12957-021-02328-w
   PubMed Web of Science ®Google Scholar
• Zhang XX, Zhan DK, YY L, et al. Glutathione peroxidase 8 as a prognostic biomarker of gastric cancer: an analysis of the cancer genome atlas (TCGA) data. Med Sci Monit. 2020;26:e921775.
   PubMed Web of Science ®Google Scholar
• Jagust P, Alcala S, Sainz B, et al. Glutathione metabolism is essential for self-renewal and chemoresistance of pancreatic cancer stem cells. World J Stem Cells. 2020;12(11):1410–1428. DOI:10.4252/wjsc.v12.i11.1410
   PubMed Web of Science ®Google Scholar
• Jia Y, Dai J, Zeng Z. Potential relationship between the selenoproteome and cancer. Mol Clin Oncol. 2020;13(6):83.
   PubMed Web of Science ®Google Scholar
• Zhang J, Liu Y, Guo Y, et al. GPX8 promotes migration and invasion by regulating epithelial characteristics in non-small cell lung cancer. Thorac Cancer. 2020;11(11):3299–3308. DOI:10.1111/1759-7714.13671
   PubMed Web of Science ®Google Scholar
• Chen H, Xu L, Shan ZL, et al. GPX8 is transcriptionally regulated by FOXC1 and promotes the growth of gastric cancer cells through activating the Wnt signaling pathway. Cancer Cell Int. 2020;20(1):596. DOI:10.1186/s12935-020-01692-z
   PubMed Web of Science ®Google Scholar
• Lee HA, Chu KB, Moon EK, et al. Glutathione peroxidase 8 suppression by histone deacetylase inhibitors enhances endoplasmic reticulum stress and cell death by oxidative stress in hepatocellular carcinoma cells. Antioxidants (Basel). 2021;10(10):1503. DOI:10.3390/antiox10101503
   PubMedGoogle Scholar
• Zahra KF, Lefter R, Ali A, et al. The Involvement of the oxidative stress status in cancer pathology: a double view on the role of the antioxidants. Oxid Med Cell Longev. 2021;2021:9965916.
   PubMed Web of Science ®Google Scholar
• Azmanova M, Pitto-Barry A. Oxidative stress in cancer therapy: friend or enemy? Chembiochem. 2022;23(10):e202100641.
   PubMed Web of Science ®Google Scholar
• Hayes JD, Dinkova-Kostova AT, Tew KD. Oxidative stress in cancer. Cancer Cell. 2020;38(2):167–197.
   PubMed Web of Science ®Google Scholar
• Yang B, Chen Q. Cross-talk between oxidative stress and m(6)A RNA methylation in cancer. Oxid Med Cell Longev. 2021;2021:6545728.
   PubMed Web of Science ®Google Scholar
• Xue D, Zhou X, Qiu J. Emerging role of NRF2 in ROS-mediated tumor chemoresistance. Biomed Pharmacother. 2020;131:110676.
   PubMed Web of Science ®Google Scholar
• Li D, Song C, Zhang J, et al. ROS and iron homeostasis dependent ferroptosis play a vital role in 5-Fluorouracil induced cardiotoxicity in vitro and in vivo. Toxicology. 2022;468:153113.
   PubMed Web of Science ®Google Scholar
• Xin X, Wen T, Gong LB, et al. Inhibition of FEN1 increases arsenic trioxide-induced ros accumulation and cell death: novel therapeutic potential for triple negative breast cancer. Front Oncol. 2020;10:425.
   PubMed Web of Science ®Google Scholar
• Zhong G, Wan F, Ning Z, et al. The protective role of autophagy against arsenic trioxide-induced cytotoxicity and ROS-dependent pyroptosis in NCTC-1469 cells. J Inorg Biochem. 2021;217:111396.
   PubMed Web of Science ®Google Scholar
• Lei G, Zhang Y, Koppula P, et al. The role of ferroptosis in ionizing radiation-induced cell death and tumor suppression. Cell Res. 2020;30(2):146–162. DOI:10.1038/s41422-019-0263-3
   PubMed Web of Science ®Google Scholar
• Yalcin Y, Tekin IO, Tigli Aydin RS. Ionizing radiation induced DNA damage via ROS production in nano ozonized oil treated B-16 melanoma and OV-90 ovarian cells. Biochem Biophys Res Commun. 2022;615:143–149.
   PubMed Web of Science ®Google Scholar
• Havaki S, Kotsinas A, Chronopoulos E, et al. The role of oxidative DNA damage in radiation induced bystander effect. Cancer Lett. 2015;356(1):43–51. DOI:10.1016/j.canlet.2014.01.023
   PubMed Web of Science ®Google Scholar
• Liu R, Bian Y, Liu L, et al. Molecular pathways associated with oxidative stress and their potential applications in radiotherapy. Int J Mol Med. 2022;49(5):65. DOI:10.3892/ijmm.2022.5121
   PubMed Web of Science ®Google Scholar
• Breau M, Houssaini A, Lipskaia L, et al. The antioxidant N-acetylcysteine protects from lung emphysema but induces lung adenocarcinoma in mice. JCI Insight. 2019;4(19):e127647. DOI:10.1172/jci.insight.127647
   PubMed Web of Science ®Google Scholar
• Hou G, Zhao X, Li L, et al. Sumoylation of YTHDF2 promotes mRNA degradation and cancer progression by increasing its binding affinity with m6A-modified mRnas. Nucleic Acids Res. 2021;49(5):2859–2877. DOI:10.1093/nar/gkab065
   PubMed Web of Science ®Google Scholar
• Zhao T, Li X, Sun D, et al. Oxidative stress: one potential factor for arsenite-induced increase of N(6)-methyladenosine in human keratinocytes. Environ Toxicol Pharmacol. 2019;69:95–103.
   PubMed Web of Science ®Google Scholar
• Wang J, Ishfaq M, Xu L, et al. Mettl3/m(6)a/miRNA-873-5p attenuated oxidative stress and apoptosis in colistin-induced kidney injury by modulating Keap1/Nrf2 pathway. Front Pharmacol. 2019;10:517.
   PubMed Web of Science ®Google Scholar
• Fu Y, Zhuang X. M(6)a-binding YTHDF proteins promote stress granule formation. Nat Chem Biol. 2020;16(9):955–963.
   PubMed Web of Science ®Google Scholar
• Chen X, Yi C, Yang MJ, et al. Metabolomics study reveals the potential evidence of metabolic reprogramming towards the Warburg effect in precancerous lesions. J Cancer. 2021;12(5):1563–1574. DOI:10.7150/jca.54252
   PubMed Web of Science ®Google Scholar
• Chang SE, Foster S, Betts D, et al. DOK, a cell line established from human dysplastic oral mucosa, shows a partially transformed non-malignant phenotype. Int J Cancer. 1992;52(6):896–902. DOI:10.1002/ijc.2910520612
   PubMed Web of Science ®Google Scholar
• Zhang Z, Wang Q, Zhang M, et al. Comprehensive analysis of the transcriptome-wide m6A methylome in colorectal cancer by MeRIP sequencing. Epigenetics. 2021;16(4):425–435. DOI:10.1080/15592294.2020.1805684
   PubMed Web of Science ®Google Scholar
• Han Z, Yang B, Wang Q, et al. Comprehensive analysis of the transcriptome-wide m(6)A methylome in invasive malignant pleomorphic adenoma. Cancer Cell Int. 2021;21(1):142. DOI:10.1186/s12935-021-01839-6
   PubMed Web of Science ®Google Scholar
• Sasada S, Miyata Y, Tsutani Y, et al. Metabolomic analysis of dynamic response and drug resistance of gastric cancer cells to 5-fluorouracil. Oncol Rep. 2013;29(3):925–931. DOI:10.3892/or.2012.2182
   PubMed Web of Science ®Google Scholar
• Chen S, Zhou Y, Chen Y, et al. Fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics. 2018;34(17):i884–90. DOI:10.1093/bioinformatics/bty560
   PubMed Web of Science ®Google Scholar
• de Sena Brandine G, Smith AD. Falco: high-speed FastQC emulation for quality control of sequencing data. F1000Res. 2019;8:1874.
   PubMedGoogle Scholar
• Wang L, Wang S, Li W. RSeQC: quality control of RNA-seq experiments. Bioinformatics. 2012;28(16):2184–2185.
   PubMed Web of Science ®Google Scholar
• Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods. 2015;12(4):357–360.
   PubMed Web of Science ®Google Scholar
• Meng J, Lu Z, Liu H, et al. A protocol for RNA methylation differential analysis with MeRIP-Seq data and exomePeak R/Bioconductor package. Methods. 2014;69(3):274–281. DOI:10.1016/j.ymeth.2014.06.008
   PubMed Web of Science ®Google Scholar
• Wang K, Li M, Hakonarson H. ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res. 2010;38(16):e164.
   PubMed Web of Science ®Google Scholar
• Bailey TL, Boden M, Buske FA, et al. MEME SUITE: tools for motif discovery and searching. Nucleic Acids Res. 2009;37(Web Server):W202–8. DOI:10.1093/nar/gkp335
   PubMedGoogle Scholar
• Pertea M, Pertea GM, Antonescu CM, et al. StringTie enables improved reconstruction of a transcriptome from RNA-seq reads. Nat Biotechnol. 2015;33(3):290–295. DOI:10.1038/nbt.3122
   PubMed Web of Science ®Google Scholar
• Robinson MD, McCarthy DJ, Smyth GK. edgeR: a Bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 2010;26(1):139–140.
   PubMed Web of Science ®Google Scholar
• Huang da W, Sherman BT, RA L. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009;4(1):44–57.
   PubMed Web of Science ®Google Scholar
• Ding L, Wang R, Zheng Q, et al. circPDE5A regulates prostate cancer metastasis via controlling WTAP-dependent N6-methyladenisine methylation of EIF3C mRNA. J Exp Clin Cancer Res. 2022;41(1):187. DOI:10.1186/s13046-022-02391-5
   PubMed Web of Science ®Google Scholar
• Yu F, Wei J, Cui X, et al. Post-translational modification of RNA m6A demethylase ALKBH5 regulates ROS-induced DNA damage response. Nucleic Acids Res. 2021;49(10):5779–5797. DOI:10.1093/nar/gkab415
   PubMed Web of Science ®Google Scholar
• Fuhrmann DC, Brune B. Mitochondrial composition and function under the control of hypoxia. Redox Biol. 2017;12:208–215.
   PubMed Web of Science ®Google Scholar
• Wang YJ, Yang B, Lai Q, et al. Reprogramming of m 6 a epitranscriptome is crucial for shaping of transcriptome and proteome in response to hypoxia. RNA Biol. 2021;18(1):131–143. DOI:10.1080/15476286.2020.1804697
   PubMed Web of Science ®Google Scholar
• Zhang C, Samanta D, Lu H, et al. Hypoxia induces the breast cancer stem cell phenotype by HIF-dependent and ALKBH5-mediated m 6 A-demethylation of NANOG mRNA. Proc Natl Acad Sci U S A. 2016;113(14):E2047–56. DOI:10.1073/pnas.1602883113
   PubMed Web of Science ®Google Scholar
• Shi Y, Fan S, Wu M, et al. YTHDF1 links hypoxia adaptation and non-small cell lung cancer progression. Nat Commun. 2019;10(1):4892. DOI:10.1038/s41467-019-12801-6
   PubMed Web of Science ®Google Scholar
• Fan Z, Yang G, Zhang W, et al. Hypoxia blocks ferroptosis of hepatocellular carcinoma via suppression of METTL14 triggered YTHDF2-dependent silencing of SLC7A11. J Cell Mol Med. 2021;25(21):10197–10212. DOI:10.1111/jcmm.16957
   PubMed Web of Science ®Google Scholar
• Shan L, Lu Y, Song Y, et al. Identification of nine m6A-related long noncoding RNAs as prognostic signatures associated with oxidative stress in oral cancer based on data from the cancer genome atlas. Oxid Med Cell Longev. 2022;2022:9529814.
   PubMed Web of Science ®Google Scholar
• Wu J, Wang X, Li X. N6-methyladenosine methylation regulator FTO promotes oxidative stress and induces cell apoptosis in ovarian cancer. Epigenomics. 2022;14(23):1509–1522.
   PubMed Web of Science ®Google Scholar
• Hou L, Li S, Li S, et al. FTO inhibits oxidative stress by mediating m6A demethylation of Nrf2 to alleviate cerebral ischemia/reperfusion injury. J Physiol Biochem. 2023;79(1):133–146. DOI:10.1007/s13105-022-00929-x
   PubMed Web of Science ®Google Scholar
• Ma L, Chen T, Zhang X, et al. The m(6)A reader YTHDC2 inhibits lung adenocarcinoma tumorigenesis by suppressing SLC7A11-dependent antioxidant function. Redox Biol. 2021;38:101801.
   PubMed Web of Science ®Google Scholar