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Short Communication

Highly efficient CRISPR/Cas9-RNP mediated CaPAD1 editing in protoplasts of three pepper (Capsicum annuum L.) cultivars

, , , & ORCID Icon
Article: 2383822 | Received 22 May 2024, Accepted 17 Jul 2024, Published online: 25 Jul 2024

References

  • Mateos RM, Jiménez A, Román P, Romojaro F, Bacarizo S, Leterrier M, Gómez M, Sevilla F, Del Río LA, Corpas FJ, et al. Antioxidant systems from pepper (Capsicum annuum L.): involvement in the response to temperature changes in ripe fruits. Int J Mol Sci. 2013;14(5):9556–6. doi:10.3390/ijms14059556.
  • Barik S, Ponnam N, Reddy AC, Dc LR, Saha K, Acharya GC, Reddy M. Breeding peppers for industrial uses: progress and prospects. Ind Crops Prod. 2022;178:114626. doi:10.1016/j.indcrop.2022.114626.
  • Harn CH, Lee YH, Kim JY, Kim HS, Min J, Choi SH, Yang SK. Mass production method for developing pepper transgenic plants by callus induction (KR patent no. 1005224370000). Korean Intellectual Property Office; 2005. https://engpat.kipris.or.kr/engpat/biblioa.do?method=biblioFrame&start=biblio&link=N&rvtExtend=N.
  • Hatfield JL, Prueger JH. Temperature extremes: effect on plant growth and development. Weather Clim Extremes. 2015;10:4–10. doi:10.1016/j.wace.2015.08.001.
  • Oh SY, Koh SC. Fruit development and quality of hot pepper (Capsicum annuum L.) under various temperature regimes. Hortic Sci Technol. 2019;37(3):313–321. doi:10.7235/HORT.20190032.
  • Erickson AN, Markhart AH. Flower developmental stage and organ sensitivity of bell pepper (Capsicum annuum L.) to elevated temperature. Plant Cell Environ. 2002;25(1):123–130. doi:10.1046/j.0016-8025.2001.00807.x.
  • Gorguet B, van Heusden AW, Lindhout P. Parthenocarpic fruit development in tomato. Plant Biol (Stuttg). 2005;7(2):131–139. doi:10.1055/s-2005-837494.
  • Mubarok S, Jadid N, Widiastuti A, Derajat Matra D, Budiarto R, Lestari FW, Nuraini A, Suminar E, Pradana Nur Rahmat B, Ezura H. Parthenocarpic tomato mutants, iaa9-3 and iaa9-5, show plant adaptability and fruiting ability under heat-stress conditions. Front Plant Sci. 2023;14:1090774. doi:10.3389/fpls.2023.1090774.
  • Matsuo S, Miyatake K, Endo M, Urashimo S, Kawanishi T, Negoro S, Shimakoshi S, Fukuoka H. Loss of function of the pad-1 aminotransferase gene, which is involved in auxin homeostasis, induces parthenocarpy in Solanaceae plants. Proc Natl Acad Sci USA. 2020;117(23):12784–12790. doi:10.1073/pnas.2001211117.
  • Kim H, Choi J, Won KH. A stable DNA-free screening system for CRISPR/RNPs-mediated gene editing in hot and sweet cultivars of Capsicum annuum. BMC Plant Biol. 2020;20(1):449. doi:10.1186/s12870-020-02665-0.
  • Lee JH, Venkatesh J, Jo J, Jang S, Kim GW, Kim JM, Han K, Ro N, Lee HY, Kwon JK, et al. High-quality chromosome-scale genomes facilitate effective identification of large structural variations in hot and sweet peppers. Hortic Res. 2022;9:uhac210. doi:10.1093/hr/uhac210.
  • Yun S, Kim H. Insight into the phylogenetic relationships and evolutionary history of pepper cultivars (Capsicum annuum L.) through comparative analyses of plastomes. Horticulturae. 2023;9(10):1092. doi:10.3390/horticulturae9101092.
  • Bae S, Park J, Kim J-S. Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics. 2014;30(10):1473–1475. doi:10.1093/bioinformatics/btu048.
  • Park J, Bae S, Kim J-S. Cas-designer: a web-based tool for choice of CRISPR-Cas9 target sites. Bioinformatics. 2015;31(24):4014–4016. doi:10.1093/bioinformatics/btv537.