Physiological and biochemical markers for screening salt tolerant quinoa genotypes at early seedling stage

References

• Abdelly C, Barhoumi Z, Ghnaya T, Debez A, Ben Hamed K, Ksouri R, Talbi O, Zribi F, Ouerghi Z, Smaoui A, et al. 2006. Potential utilisation of halophytes for the rehabilitation and valorisation of salt-affected areas in Tunisia. Biosaline Agric Salinity Tolerance in Plants. Birkhäuser Basel, Basel. 163–172. doi:10.1007/3-7643-7610-4_18.
   Google Scholar
• Abdelly C, Debez A, Smaoui A, Grignon C. 2011. Halophyte-fodder species association may improve nutrient availability and biomass production of the Sabkha ecosystem. In: Öztürk M., Böer B., Barth H.-J., Clüsener-Godt M., Khan M.A., Breckle S.-W., editor. Sabkha ecosystems: volume III: Africa and Southern Europe. Dordrecht: Springer Netherlands; p. 85–94. doi: 10.1007/978-90-481-9673-9_10
   Google Scholar
• Adolf VI, Shabala S, Andersen MN, Razzaghi F, Jacobsen S-E. 2012. Varietal differences of quinoa’s tolerance to saline conditions. Plant Soil. 357:117–129. doi:10.1007/s11104-012-1133-7.
   Web of Science ®Google Scholar
• Aebi H. 1984. [13] Catalase in vitro. Methods in Enzymology. 105:121–126. doi:10.1016/S0076-6879(84)05016-3.
   PubMed Web of Science ®Google Scholar
• Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol. 55:373–399. doi:10.1146/annurev.arplant.55.031903.141701.
   PubMed Web of Science ®Google Scholar
• Arndt SK, Arampatsis C, Foetzki A, Li X, Zeng F, Zhang X. 2004. Contrasting patterns of leaf solute accumulation and salt adaptation in four phreatophytic desert plants in a hyperarid desert with saline groundwater. J Arid Environ. 59:259–270. doi:10.1016/j.jaridenv.2004.01.017.
   Web of Science ®Google Scholar
• Ashraf M, Foolad MR. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot. 59:206–216. doi:10.1016/j.envexpbot.2005.12.006.
   Web of Science ®Google Scholar
• Ashraf M, Harris PJC. 2004. Potential biochemical indicators of salinity tolerance in plants. Plant Sci. 166:3–16. doi:10.1016/j.plantsci.2003.10.024.
   Web of Science ®Google Scholar
• Ashraf M, Harris PJC. 2013. Photosynthesis under stressful environments: An overview. Photosynthetica. 51:163–190. doi:10.1007/s11099-013-0021-6.
   Web of Science ®Google Scholar
• Banerjee K, Gatti RC, Mitra A. 2017. Climate change-induced salinity variation impacts on a stenoecious mangrove species in the Indian Sundarbans. Ambio. 46:492–499. doi:10.1007/s13280-016-0839-9.
   PubMed Web of Science ®Google Scholar
• Bano S, Ashraf M, Akram NA. 2014. Salt stress regulates enzymatic and nonenzymatic antioxidative defense system in the edible part of carrot (Daucus carota L.). J Plant Interact. 9:324–329. doi:10.1080/17429145.2013.832426.
   Web of Science ®Google Scholar
• Bates LS, Waldren RP, Teare ID. 1973. Rapid determination of free proline for water-stress studies. Plant Soil. 39:205–207. doi:10.1007/BF00018060.
   Web of Science ®Google Scholar
• Ben Amor N, Ben Hamed K, Debez A, Grignon C, Abdelly C. 2005. Physiological and antioxidant responses of the perennial halophyte Crithmum maritimum to salinity. Plant Sci. 168:889–899. doi:10.1016/j.plantsci.2004.11.002.
   Web of Science ®Google Scholar
• Bendaly A, Messedi D, Smaoui A, Ksouri R, Bouchereau A, Abdelly C. 2016. Physiological and leaf metabolome changes in the xerohalophyte species Atriplex halimus induced by salinity. Plant Physiol Biochem. 103:208–218. doi:10.1016/j.plaphy.2016.02.037.
   PubMed Web of Science ®Google Scholar
• Bertero HD, de la Vega AJ, Correa G, Jacobsen SE, Mujica A. 2004. Genotype and genotype-by-environment interaction effects for grain yield and grain size of quinoa (Chenopodium quinoa Willd.) as revealed by pattern analysis of international multi-environment trials. Field Crops Res. 89:299–318. doi:10.1016/j.fcr.2004.02.006.
   Web of Science ®Google Scholar
• Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248–254. doi:10.1016/0003-2697(76)90527-3.
   PubMed Web of Science ®Google Scholar
• Cuartero J, Romero-Aranda R, Yeo AR, Flowers TJ. 2002. Variability for some physiological characters affecting salt tolerance in tomato. Acta Hortic. 573:435–441. doi:10.17660/ActaHortic.2002.573.52.
   Google Scholar
• Dajié Z, Stajkovié M, Jakovljevié M. 1997. An ecophysiological study of Suaeda maritima (Chenopodiaceae) in Serbia. Bocconea. 5:511–516.
   Google Scholar
• Debez A, Ben Hamed K, Grignon C, Abdelly C. 2004. Salinity effects on germination, growth, and seed production of the halophyte Cakile maritima. Plant Soil. 262:179–189. doi:10.1023/B:PLSO.0000037034.47247.67.
   Web of Science ®Google Scholar
• Demiral T, Türkan İ. 2005. Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ Exp Bot. 53:247–257. doi:10.1016/j.envexpbot.2004.03.017.
   Web of Science ®Google Scholar
• Eisa S, Hussin S, Geissler N, Koyro H. 2012. Effect of NaCl salinity on water relations, photosynthesis and chemical composition of Quinoa (Chenopodium quinoa Willd.) as a potential cash crop halophyte. Aust J Crop Sci. 6:357–368.
   Google Scholar
• Farhat N, Rabhi M, Falleh H, Lengliz K, Smaoui A, Abdelly C, Lachaâl M, Karray-Bouraoui N. 2013. Interactive effects of excessive potassium and Mg deficiency on safflower. Acta Physiol Plant. 35:2737–2745. doi:10.1007/s11738-013-1306-x.
   Web of Science ®Google Scholar
• Flowers TJ, Colmer TD. 2008. Salinity tolerance in halophytes. New Phytol. 179:945–963. doi:10.1111/j.1469-8137.2008.02531.x.
   PubMed Web of Science ®Google Scholar
• Flowers TJ, Colmer TD. 2015. Plant salt tolerance: adaptations in halophytes. Ann Bot. 115:327–331. doi:10.1093/aob/mcu267.
   PubMed Web of Science ®Google Scholar
• Flowers TJ, Munns R, Colmer TD. 2015. Sodium chloride toxicity and the cellular basis of salt tolerance in halophytes. Ann Bot. 115:419–431. doi:10.1093/aob/mcu217.
   PubMed Web of Science ®Google Scholar
• Foyer CH, Noctor G. 2005. Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ. 28:1056–1071. doi:10.1111/j.1365-3040.2005.01327.x.
   Web of Science ®Google Scholar
• Gagneul D, Aïnouche A, Duhazé C, Lugan R, Larher FR, Bouchereau A. 2007. A reassessment of the function of the so-called compatible solutes in the halophytic Plumbaginaceae Limonium latifolium. Plant Physiol. 144:1598–1611. doi:10.1104/pp.107.099820.
   PubMed Web of Science ®Google Scholar
• Gill SS, Tuteja N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 48:909–930. doi:10.1016/j.plaphy.2010.08.016.
   PubMed Web of Science ®Google Scholar
• Goussi R, Manaa A, Derbali W, Cantamessa S, Abdelly C, Barbato R. 2018. Comparative analysis of salt stress, duration and intensity, on the chloroplast ultrastructure and photosynthetic apparatus in Thellungiella salsuginea. J Photochem Photobiol, B. 183:275–287. doi:10.1016/j.jphotobiol.2018.04.047.
   PubMed Web of Science ®Google Scholar
• Hariadi Y, Marandon K, Tian Y, Jacobsen S-E, Shabala S. 2011. Ionic and osmotic relations in quinoa (Chenopodium quinoa Willd.) plants grown at various salinity levels. J Exp Bot. 62:185–193. doi:10.1093/jxb/erq257.
   PubMed Web of Science ®Google Scholar
• Harrouni MC, Daoud S, Koyro H-W. 2003. Effect of seawater irrigation on biomass production and ion composition of seven halophytic species in Morocco. In: Lieth H., Mochtchenko M, editor. Cash crop halophytes: recent studies. Tasks for vegetation science, vol 38. Dordrecht: Springer; p. 59–70. doi: 10.1007/978-94-017-0211-9_6
   Google Scholar
• Hewitt EJ. 1966. Sand and water culture methods used in the study of plant nutrition. Tech. Commun., 22, Commonwealth Agric. Bureau, Farnham Royal, 2nd edn, 547 pp.
   Google Scholar
• Hussain MI, Al- Dakheel AJ, Reigosa MJ. 2018. Genotypic differences in agro-physiological, biochemical and isotopic responses to salinity stress in quinoa (Chenopodium quinoa Willd.) plants: Prospects for salinity tolerance and yield stability. Plant Physiol Biochem. 129:411–420. doi:10.1016/j.plaphy.2018.06.023.
   PubMed Web of Science ®Google Scholar
• Iqbal H, Yaning C, Waqas M, Shareef M, Raza ST. 2018. Differential response of quinoa genotypes to drought and foliage-applied H2O2 in relation to oxidative damage, osmotic adjustment and antioxidant capacity. Ecotoxicol Environ Saf. 164:344–354. doi:10.1016/j.ecoenv.2018.08.004.
   PubMed Web of Science ®Google Scholar
• Jacobsen S-E. 2014. New climate-proof cropping systems in dry areas of the Mediterranean region. J Agron Crop Sci. 200:399–401. doi:10.1111/jac.12080.
   Web of Science ®Google Scholar
• Jacobsen S-E, Mujica A, Jensen CR. 2003. The resistance of quinoa (Chenopodium quinoa Willd.) to adverse abiotic factors. Food Rev Int. 19:99–109. doi:10.1081/FRI-120018872.
   Web of Science ®Google Scholar
• Jacobsen S-E, Quispe H, Mujica A. 2001. Quinoa: an alternative crop for saline soils in the Andes, Scientists and Farmer-Partners in Research for the 21st Century. CIP Program Report 1999–2000, pp. 403–408.
   Google Scholar
• Jacobsen S-E, Sørensen M, Pedersen SM, Weiner J. 2013. Feeding the world: genetically modified crops versus agricultural biodiversity. Agron Sustainable Dev. 33:651–662. doi:10.1007/s13593-013-0138-9.
   Web of Science ®Google Scholar
• James RA, Rivelli AR, Munns R, Caemmerer Sv. 2002. Factors affecting CO2 assimilation, leaf injury and growth in salt-stressed durum wheat. Funct Plant Biol. 29:1393–1403. doi:10.1071/FP02069.
   PubMed Web of Science ®Google Scholar
• Jamil A, Riaz S, Ashraf M, Foolad MR. 2011. Gene expression profiling of plants under salt stress. Crit Rev Plant Sci. 30:435–458. doi:10.1080/07352689.2011.605739.
   Web of Science ®Google Scholar
• Karyotis T, Iliadis C, Noulas C, Mitsibonas T. 2003. Preliminary research on seed production and nutrient content for certain quinoa varieties in a saline–sodic soil. J Agron Crop Sci. 189:402–408. doi:10.1046/j.0931-2250.2003.00063.x.
   Web of Science ®Google Scholar
• Khan AM, Ungar IA, Showalter AM. 2000. The effect of salinity on the growth, water status, and ion content of a leaf succulent perennial halophyte, Suaeda fruticosa (L.) Forssk. J Arid Environ. 45:73–84. doi:10.1006/jare.1999.0617.
   Web of Science ®Google Scholar
• Koyro H-W. 2003. Study of potential cash crop halophytes by a quick check system: determination of the threshold of salinity tolerance and the ecophysiological demands. In: Lieth H., Mochtchenko M., editor. Cash crop halophytes: recent studies. Tasks for vegetation science, vol 38. Dordrecht: Springer; p. 5–17. doi: 10.1007/978-94-017-0211-9_1
   Google Scholar
• Koyro HW. 2006. Effect of salinity on growth, photosynthesis, water relations and solute composition of the potential cash crop halophyte Plantago coronopus (L.). Environ Exp Bot. 56:136–146. doi:10.1016/j.envexpbot.2005.02.001.
   Web of Science ®Google Scholar
• Koyro H-W, Eisa SS. 2008. Effect of salinity on composition, viability and germination of seeds of Chenopodium quinoa Willd. Plant Soil. 302:79–90. doi:10.1007/s11104-007-9457-4.
   Web of Science ®Google Scholar
• Koyro H-W, Geissler N, Hussin S, Huchzermeyer B. 2006. Mechanisms of cash crop halophytes to maintain yields and reclaim saline soils in arid areas. In: Khan M.A., Weber D.J, editor. Ecophysiology of high salinity tolerant plants. Tasks for vegetation science, vol 40. Dordrecht: Springer; p. 345–366. doi: 10.1007/1-4020-4018-0_22
   Google Scholar
• Lichtenthaler HK. 1987. [34] Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol. 148:350–382. doi:10.1016/0076-6879(87)48036-1.
   Web of Science ®Google Scholar
• Loreto F, Velikova V. 2001. Isoprene produced by leaves protects the photosynthetic apparatus against ozone damage, quenches ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiol. 127:1781–1787. doi:10.1104/pp.010497.
   PubMed Web of Science ®Google Scholar
• Lutts S, Kinet JM, Bouharmont J. 1996. Effects of salt stress on growth, mineral nutrition and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa L.) cultivars differing in salinity resistance. Plant Growth Regul. 19:207–218. doi:10.1007/BF00037793.
   Web of Science ®Google Scholar
• Manaa A, Ben Ahmed H, Valot B, Bouchet J-P, Aschi-Smiti S, Causse M, Faurobert M. 2011. Salt and genotype impact on plant physiology and root proteome variations in tomato. J Exp Bot. 62:2797–2813. doi:10.1093/jxb/erq460.
   PubMed Web of Science ®Google Scholar
• Manaa A, Gharbi E, Mimouni H, Wasti S, Aschi-Smiti S, Lutts S, Ben Ahmed H. 2014a. Simultaneous application of salicylic acid and calcium improves salt tolerance in two contrasting tomato (Solanum lycopersicum) cultivars. S Afr J Bot. 95:32–39. doi:10.1016/j.sajb.2014.07.015.
   Web of Science ®Google Scholar
• Manaa A, Goussi R, Derbali W, Cantamessa S, Abdelly C, Barbato R. 2019. Salinity tolerance of quinoa (Chenopodium quinoa Willd.) as assessed by chloroplast ultrastructure and photosynthetic performance. Environmental and Experimental Botany. 162:103–114. doi:10.1016/j.envexpbot.2019.02.012.
   Web of Science ®Google Scholar
• Manaa A, Mimouni H, Terras A, Chebil F, Wasti S, Gharbi E, Ben Ahmed H. 2014b. Superoxide dismutase isozyme activity and antioxidant responses of hydroponically cultured Lepidium sativum L. to NaCl stress. J Plant Interact. 9:440–449. doi:10.1080/17429145.2013.850596.
   Web of Science ®Google Scholar
• Mansour MMF, Ali EF. 2017. Evaluation of proline functions in saline conditions. Phytochem. 140:52–68. doi:10.1016/j.phytochem.2017.04.016.
   PubMed Web of Science ®Google Scholar
• Misra N, Gupta AK. 2005. Effect of salt stress on proline metabolism in two high yielding genotypes of green gram. Plant Sci. 169:331–339. doi:10.1016/j.plantsci.2005.02.013.
   Web of Science ®Google Scholar
• Mittal S, Kumari N, Sharma V. 2012. Differential response of salt stress on Brassica juncea: photosynthetic performance, pigment, proline, D1 and antioxidant enzymes. Plant Physiol Biochem. 54:17–26. doi:10.1016/j.plaphy.2012.02.003.
   PubMed Web of Science ®Google Scholar
• Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7:405–410. doi:10.1016/S1360-1385(02)02312-9.
   PubMed Web of Science ®Google Scholar
• Munns R, Termaat A. 1986. Whole-plant responses to salinity. Aust J Plant Physiol. 13:143–160. doi:10.1071/PP9860143.
   Google Scholar
• Munns R, Tester M. 2008. Mechanisms of salinity tolerance. Annu Rev Plant Biol. 59:651–681. doi:10.1146/annurev.arplant.59.032607.092911.
   PubMed Web of Science ®Google Scholar
• Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant & Cell Physiol. 22:867–888. doi:10.1093/oxfordjournals.pcp.a076232.
   Web of Science ®Google Scholar
• Negrão S, Schmöckel SM, Tester M. 2017. Evaluating physiological responses of plants to salinity stress. Ann Bot. 119:1–11. doi:10.1093/aob/mcw191.
   PubMed Web of Science ®Google Scholar
• Nikalje CG, Nikam TD, Suprasanna P. 2017. Looking at halophytic adaptation to high salinity through genomics Landscape. Curr Genomics. 18:542–552. doi:10.2174/1389202918666170228143007.
   PubMed Web of Science ®Google Scholar
• Oukarroum A, Bussotti F, Goltsev V, Kalaji HM. 2015. Correlation between reactive oxygen species production and photochemistry of photosystems I and II in Lemna gibba L. plants under salt stress. Environ Exp Bot. 109:80–88. doi:10.1016/j.envexpbot.2014.08.005.
   Web of Science ®Google Scholar
• Rao MKV, Sresty TVS. 2000. Antioxidative parameters in the seedlings of pigeonpea (Cajanus cajan (L.) Millspaugh) in response to Zn and Ni stresses. Plant Sci. 157:113–128. doi:10.1016/S0168-9452(00)00273-9.
   PubMed Web of Science ®Google Scholar
• Redondo-Gómez S, Mateos-Naranjo E, Davy AJ, Fernandez-Munoz F, Castellanos EM, Luque T, Figueroa ME. 2007. Growth and photosynthetic responses to salinity of the salt-marsh shrub Atriplex portulacoides. Ann Bot. 100:555–563. doi:10.1093/aob/mcm119.
   PubMed Web of Science ®Google Scholar
• Redondo-Gómez S, Mateos-Naranjo E, Figueroa ME, Davy AJ. 2010. Salt stimulation of growth and photosynthesis in an extreme halophyte, Arthrocnemum macrostachyum. Plant Biol. 12:79–87. doi:10.1111/j.1438-8677.2009.00207.x.
   PubMed Web of Science ®Google Scholar
• Redondo-Gómez S, Wharmby C, Castillo JM, Mateos-Naranjo E, Luque CJ, de Cires A, Luque T, Davy AJ, Figueroa ME. 2006. Growth and photosynthetic responses to salinity in an extreme halophyte, Sarcocornia fruticosa. Physiol Plant. 128:116–124. doi:10.1111/j.1399-3054.2006.00719.x.
   Web of Science ®Google Scholar
• Rozema J, Flowers T. 2008. Crops for a salinized world. Science. 322:1478–1480. doi:10.1126/science.1168572.
   PubMed Web of Science ®Google Scholar
• Ruiz KB, Biondi S, Oses R, Acuña-Rodríguez IS, Antognoni F, Martinez-Mosqueira EA, Coulibaly A, Canahua-Murillo A, Pinto M, Zurita-Silva A, et al. 2014. Quinoa biodiversity and sustainability for food security under climate change. A review. Agron Sustainable Dev. 34:349–359. doi:10.1007/s13593-013-0195-0.
   Web of Science ®Google Scholar
• Shabala S. 2013. Learning from halophytes: physiological basis and strategies to improve abiotic stress tolerance in crops. Ann Bot. 112:1209–1221. doi:10.1093/aob/mct205.
   PubMed Web of Science ®Google Scholar
• Shabala S, Hariadi Y, Jacobsen S-E. 2013. Genotypic difference in salinity tolerance in quinoa is determined by differential control of xylem Na+ loading and stomatal density. J Plant Physiol. 170:906–914. doi:10.1016/j.jplph.2013.01.014.
   PubMed Web of Science ®Google Scholar
• Song J, Feng G, Tian C-Y, Zhang F-S. 2006. Osmotic adjustment traits of Suaeda physophora, Haloxylon ammodendron and Haloxylon persicum in field or controlled conditions. Plant Sci. 170:113–119. doi:10.1016/j.plantsci.2005.08.004.
   Web of Science ®Google Scholar
• Sosa-Zuniga V, Brito V, Fuentes F, Steinfort U. 2017. Phenological growth stages of quinoa (Chenopodium quinoa) based on the BBCH scale. Ann Appl Biol. 171:117–124. doi:10.1111/aab.12358.
   Web of Science ®Google Scholar
• Souid A, Bellani L, Magné C, Zorrig W, Smaoui A, Abdelly C, Longo V, Ben Hamed K. 2018. Physiological and antioxidant responses of the sabkha biotope halophyte Limonium delicatulum to seasonal changes in environmental conditions. Plant Physiol Biochem. 123:180–191. doi:10.1016/j.plaphy.2017.12.008.
   PubMed Web of Science ®Google Scholar
• Sudhir P, Murthy SDS. 2004. Effects of salt stress on basic processes of photosynthesis. Photosynthetica. 42:481–486. doi:10.1007/S11099-005-0001-6.
   Web of Science ®Google Scholar
• Vineis P, Chan Q, Khan A. 2011. Climate change impacts on water salinity and health. J Epidemiol Glob Health. 1:5–10. doi:10.1016/j.jegh.2011.09.001.
   PubMedGoogle Scholar
• Vineis P, Khan A. 2012. Climate change-induced salinity threatens health. Science. 338:1028–1029. doi:10.1126/science.338.6110.1028-b.
   PubMed Web of Science ®Google Scholar
• Waqas M, Yaning C, Iqbal H, Shareef M, Rehman H, Yang Y. 2017. Paclobutrazol improves salt tolerance in quinoa: Beyond the stomatal and biochemical interventions. J Agron Crop Sci. 203:315–322. doi:10.1111/jac.12217.
   Web of Science ®Google Scholar
• Waqas M, Yaning C, Iqbal H, Shareef M, ur Rehman H, Iqbal S, Mahmood S. 2019. Soil drenching of paclobutrazol: An efficient way to improve quinoa performance under salinity. Physiol Plant. 165:219–231. doi:10.1111/ppl.12820.
   PubMed Web of Science ®Google Scholar
• Willekens H, Chamnongpol S, Davey M, Schraudner M, Langebartels C, Van Montagu M, Van Camp W. 1997. Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. EMBO J. 16:4806–4816. doi:10.1093/emboj/16.16.4806.
   PubMed Web of Science ®Google Scholar
• Wolf B. 1982. A comprehensive system of leaf analyses and its use for diagnosing crop nutrient status. Commun Soil Sci Plant Anal. 13:1035–1059. doi:10.1080/00103628209367332.
   Web of Science ®Google Scholar
• Yeo A. 1998. Molecular biology of salt tolerance in the context of whole-plant physiology. J Exp Bot. 49:915–929. doi:10.1093/jxb/49.323.915.
   Web of Science ®Google Scholar
• Zhang H, Zhao K. 1998. Effects of salt and water stresses on osmotic adjustment of Suaeda salsa seedlings. Acta Bot Sin. 40:56–61.
   Google Scholar
• Zhao X, Wang W, Zhang F, Deng J, Li Z, Fu B. 2014. Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage. PLoS One. 9:e108020. doi:10.1371/journal.pone.0108020.
   PubMed Web of Science ®Google Scholar
• Zurita-Silva A, Fuentes F, Zamora P, Jacobsen S-E, Schwember AR. 2014. Breeding quinoa (Chenopodium quinoa Willd.): potential and perspectives. Mol Breed. 34:13–30. doi:10.1007/s11032-014-0023-5.
   Web of Science ®Google Scholar