Characterization of actinomycetes isolates for plant growth promoting traits and their effects on drought tolerance in maize

References

• Adegboye MF, Babalola OO. 2013. Actinomycetes: a yet inexhaustive source of bioactive secondary metabolites. In: Mendez-Vilas A, editor. Microbial pathogens and strategies for combating them: science, technology and education. Microbiology Book Series - 4, Vol. 2. December 2013 ed. Spain: Formatex Research Center. p. 786-795. ISBN-13. 978-84-942134-0-3.
   Google Scholar
• Adegboye MF, Babalola OO. 2015. Evaluation of antibiotic biosynthetic potential of actinomycete isolates to produce antimicrobial agents. British Microbiol Res J. 7(5):243–254. doi:10.9734/BMRJ/2015/14627.
   Google Scholar
• Alexander D, Zuberer D. 1991. Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol Fertil Soils. 12:39–45. doi: 10.1007/BF00369386
   Web of Science ®Google Scholar
• Ali SZ, Sandhya V, Venkateswar Rao L. 2014. Isolation and characterization of drought-tolerant ACC deaminase and exopolysaccharide-producing fluorescent Pseudomonas spp. Ann Microbiol. 64:493–502. doi: 10.1007/s13213-013-0680-3
   Web of Science ®Google Scholar
• Arshad M, Shaharoona B, Mahmood T. 2008. Inoculation with Pseudomonas spp. containing ACC-deaminase partially eliminates the effects of drought stress on growth, yield, and ripening of pea (Pisum sativum L.). Pedosphere. 18:611–620. doi: 10.1016/S1002-0160(08)60055-7
   Web of Science ®Google Scholar
• Ashraf M. 2010. Inducing drought tolerance in plants: recent advances. Biotechnol Adv. 28:169–183. doi: 10.1016/j.biotechadv.2009.11.005
   PubMed Web of Science ®Google Scholar
• Babalola OO. 2010. Beneficial bacteria of agricultural importance. Biotechnol Lett. 32(11):1559–1570. ISSN: 0141-5492 E-ISSN: 1573-6776. doi:10.1007/s10529-010-0347-0.
   PubMed Web of Science ®Google Scholar
• Babalola OO, Glick BR. 2012. The use of microbial inoculants in African agriculture: current practice and future prospects. J Food Agric Environ (Finland). 10(3 & 4):540–549. (ISSN: 1459-0263).
   Google Scholar
• Bakker AW, Schippers B. 1987. Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp-mediated plant growth-stimulation. Soil Biol Biochem. 19:451–457. doi: 10.1016/0038-0717(87)90037-X
   Web of Science ®Google Scholar
• Bardi L, Malusà E. 2012. Drought and nutritional stresses in plant: alleviating role of rhizospheric microorganisms. In: Haryana Nikhil, Punj Shreya, editors. Abiotic stress: new research. Hauppauge, NY, USA: Nova Science; p. 1–57.
   Google Scholar
• Bashan Y, De-Bashan LE. 2010. How the plant growth-promoting bacterium Azospirillum promotes plant growth-a critical assessment. In: Sparks Donald L., editor. Advances in Agronomy. Vol. 108. Academic Press; p. 77–136.
   Google Scholar
• Begum M, Rai VR, Lokesh S. 2012. Effect of plant growth promoting rhizobacteria on seed borne fungal pathogens in okra. Indian Phytopathol. 56:156–158.
   Google Scholar
• Belimov AA, Dodd IC, Hontzeas N, Theobald JC, Safronova VI, Davies WJ. 2009. Rhizosphere bacteria containing 1-aminocyclopropane-1-carboxylate deaminase increase yield of plants grown in drying soil via both local and systemic hormone signalling. New Phytol. 181:413–423. doi: 10.1111/j.1469-8137.2008.02657.x
   PubMed Web of Science ®Google Scholar
• Brimecombe MJ, De Leij FA, Lynch JM, Pinton R, Varanini Z, Nannipieri P. 2000. The effect of root exudates on rhizosphere microbial populations. Biochemistry and organic substances at the soil-plant interface. In: Pinton R, Varaninin Z, Nannipieri P, editors. The Rhizosphere, biochemistry and organic substances at the soil-plant interface. New York: Marcel Dekker. p. 95–140.
   Google Scholar
• Cassán F, Vanderleyden J, Spaepen S. 2014. Physiological and agronomical aspects of phytohormone production by model plant-growth-promoting rhizobacteria (PGPR) belonging to the genus Azospirillum. J Plant Growth Regul. 33:440–459. doi: 10.1007/s00344-013-9362-4
   Web of Science ®Google Scholar
• Chabot R, Antoun H, Cescas MP. 1993. Stimulation de la croissance du maïs et de la laitue romaine par des microorganismes dissolvant le phosphore inorganique. Can J Microbiol. 39:941–947. doi: 10.1139/m93-142
   Web of Science ®Google Scholar
• Chen Y, Rekha P, Arun A, Shen F, Lai W-A, Young C. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl Soil Ecol. 34:33–41. doi: 10.1016/j.apsoil.2005.12.002
   Web of Science ®Google Scholar
• Chukwuneme CF. 2018. Actinomycetes impacts on drought stress in maize [Dissertation - Masters]. South Africa: North-West University..
   Google Scholar
• Coleman-Derr D, Tringe SG. 2014. Building the crops of tomorrow: advantages of symbiont-based approaches to improving abiotic stress tolerance. Front Microbiol. 5:283–296. doi:10.3389/fmicb.2014.00283.
   PubMed Web of Science ®Google Scholar
• Compant S, Duffy B, Nowak J, Clément C, Barka EA. 2005. Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl Environ Microbiol. 71:4951–4959. doi: 10.1128/AEM.71.9.4951-4959.2005
   PubMed Web of Science ®Google Scholar
• Creus CM, Sueldo RJ, Barassi CA. 2004. Water relations and yield in Azospirillum-inoculated wheat exposed to drought in the field. Can J Botany. 82:273–281. doi: 10.1139/b03-119
   Google Scholar
• Delcour JA, Poutanen K, editors. 2013. Fibre-rich and wholegrain foods: improving quality. Woodhead, Cambridge. p. 1–459.
   Google Scholar
• Dimkpa C, Weinand T, Asch F. 2009. Plant-rhizobacteria interactions alleviate abiotic stress conditions. Plant Cell Environ. 32:1682–1694. doi: 10.1111/j.1365-3040.2009.02028.x
   PubMed Web of Science ®Google Scholar
• Duarah I, Deka M, Saikia N, Deka Boruah HP. 2011. Phosphate solubilizers enhance NPK fertilizer use efficiency in rice and legume cultivation. Biotechnol. 1:227–238.
   Google Scholar
• Duca D, Lorv J, Patten CL, Rose D, Glick BR. 2014. Indole-3-acetic acid in plant–microbe interactions. Antonie Van Leeuwenhoek. 106:85–125. doi:10.1007/s10482-013-0095-y.
   PubMed Web of Science ®Google Scholar
• Dworkin M, Foster J. 1958. Experiments with some microorganisms which utilize ethane and hydrogen. J Bacteriol. 75:592–603. doi: 10.1128/JB.75.5.592-603.1958
   PubMed Web of Science ®Google Scholar
• Eisenstein M. 2013. Discovery in a dry spell. Nature. 501:S7–S9. doi:10.1038/501S7a.
   PubMed Web of Science ®Google Scholar
• El-Tarabily KA. 2008. Promotion of tomato (Lycopersicon esculentum Mill.) plant growth by rhizosphere competent 1-aminocyclopropane-1-carboxylic acid deaminase-producing streptomycete actinomycetes. Plant Soil. 308:161–174. doi: 10.1007/s11104-008-9616-2
   Web of Science ®Google Scholar
• Elegba M, Rennie R. 1984. Effect of different inoculant adhesive agents on rhizobial survival, nodulation, and nitrogenase (acetylene-reducing) activity of soybeans (Glycine max (L.) Merrill). Can J Soil Sci. 64:631–636. doi: 10.4141/cjss84-063
   Web of Science ®Google Scholar
• Farooq M, Wahid A, Kobayashi N, Fujita D, Basra S. 2009. Plant drought stress: effects, mechanisms and management. Agron Sustainable Dev. 29:185–212. doi: 10.1051/agro:2008021
   Web of Science ®Google Scholar
• Figueiredo MV, Burity HA, Martínez CR, Chanway CP. 2008. Alleviation of drought stress in the common bean (Phaseolus vulgaris L.) by co-inoculation with Paenibacillus polymyxa and Rhizobium tropici. Appl Soil Ecol. 40:182–188. doi: 10.1016/j.apsoil.2008.04.005
   Web of Science ®Google Scholar
• Gerhardt KE, Huang X-D, Glick BR, Greenberg BM. 2009. Phytoremediation and rhizoremediation of organic soil contaminants: potential and challenges. Plant Sci. 176:20–30. doi: 10.1016/j.plantsci.2008.09.014
   Web of Science ®Google Scholar
• Ghorbanpour M, Hatami M. 2014. Biopriming of salvia officinalis seed with growth promoting rhizobacteria affects invigoration and germination indices. J. Biol Environ Sci. 8:29–36.
   Google Scholar
• Glick BR. 2005. Modulation of plant ethylene levels by the bacterial enzyme ACC deaminase. FEMS Microbiol Lett. 251:1–7. doi: 10.1016/j.femsle.2005.07.030
   PubMed Web of Science ®Google Scholar
• Glick BR. 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol Res. 169:30–39. doi: 10.1016/j.micres.2013.09.009
   PubMed Web of Science ®Google Scholar
• Glick BR, Todorovic B, Czarny J, Cheng Z, Duan J, McConkey B. 2007. Promotion of plant growth by bacterial ACC deaminase. Crit Rev Plant Sci. 26:227–242. doi: 10.1080/07352680701572966
   Web of Science ®Google Scholar
• Gong H, Zhu X, Chen K, Wang S, Zhang C. 2005. Silicon alleviates oxidative damage of wheat plants in pots under drought. Plant Sci. 169:313–321. doi: 10.1016/j.plantsci.2005.02.023
   Web of Science ®Google Scholar
• Gusain YS, Singh U, Sharma A. 2015. Bacterial mediated amelioration of drought stress in drought tolerant and susceptible cultivars of rice (Oryza sativa L.). Afr J Biotechnol. 14:764–773. doi: 10.5897/AJB2015.14405
   Google Scholar
• Ibarra VG, Sendón R, de Quirós AR-B. 2016. Antimicrobial food packaging based on biodegradable materials. In: Barros-Velazques Jorge, editor. Antimicrobial food packaging. San Diego, USA: Academic Press; p. 363–384.
   Google Scholar
• Idris EE, Iglesias DJ, Talon M, Borriss R. 2007. Tryptophan-dependent production of indole-3-acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42. Mol Plant-Microbe Interact. 20:619–626. doi: 10.1094/MPMI-20-6-0619
   PubMed Web of Science ®Google Scholar
• Islam MR, Madhaiyan M, Deka Boruah HP, Yim W, Lee G, Saravanan VF, Fu Q, Hu H, Sa T. 2009. Characterization of plant growth-promoting traits of free-living diazotrophic bacteria and their inoculation effects on growth and nitrogen uptake of crop plants. J Microbiol Biotechnol. 19(10):1213–1222. doi:10.4014/jmb.0903.3028.
   PubMed Web of Science ®Google Scholar
• Jongdee B, Pantuwan G, Fukai S, Fischer K. 2006. Improving drought tolerance in rainfed lowland rice: an example from Thailand. Agric Water Manag. 80:225–240. doi: 10.1016/j.agwat.2005.07.015
   Web of Science ®Google Scholar
• Khamna S, Yokota A, Peberdy JF, Lumyong S. 2010. Indole-3-acetic acid production by Streptomyces sp. isolated from some Thai medicinal plant rhizosphere soils. EurAsian J. BioSci. 4:23–32. doi: 10.5053/ejobios.2010.4.0.4
   Google Scholar
• Khantsi M, Adegboye MF, Babalola OO. 2013. 1-Aminocyclopropane-1-carboxylate deaminase activity as a marker for identifying plant-growth promoting rhizobacteria in cultivated soil. Asian Life Sci. 9:199–211. ISSN 0117-3375.
   Google Scholar
• Koussevitzky S, Suzuki N, Huntington S, Armijo L, Sha W, Cortes D, Shulaev V, Mittler R. 2008. Ascorbate peroxidase 1 plays a key role in the response of Arabidopsis thaliana to stress combination. J Biol Chem. 283:34197–34203. doi: 10.1074/jbc.M806337200
   PubMed Web of Science ®Google Scholar
• Langridge P, Reynolds MP. 2015. Genomic tools to assist breeding for drought tolerance. Curr Opin Biotechnol. 32:130–135. doi: 10.1016/j.copbio.2014.11.027
   PubMed Web of Science ®Google Scholar
• Laslo É, György É, Mara G, Tamás É, Ábrahám B, Lányi S. 2012. Screening of plant growth promoting rhizobacteria as potential microbial inoculants. Crop Prot. 40:43–48. doi: 10.1016/j.cropro.2012.05.002
   Web of Science ®Google Scholar
• Lucy M, Reed E, Glick BR. 2004. Applications of free living plant growth-promoting rhizobacteria. Antonie Van Leeuwenhoek. 86:1–25. doi: 10.1023/B:ANTO.0000024903.10757.6e
   PubMed Web of Science ®Google Scholar
• Maazou A-RS, Tu J, Qiu J, Liu Z. 2016. Breeding for drought tolerance in maize (Zea mays L.). Am J Plant Sci. 7:1858–1870. doi: 10.4236/ajps.2016.714172
   Google Scholar
• Madhaiyan M, Poonguzhali S, Sa T. 2007. Metal tolerating methylotrophic bacteria reduces nickel and cadmium toxicity and promotes plant growth of tomato (Lycopersicon esculentum L.). Chemosphere. 69:220–228. doi: 10.1016/j.chemosphere.2007.04.017
   PubMed Web of Science ®Google Scholar
• Matsukawa E, Nakagawa Y, Iimura Y, Hayakawa M. 2007. Stimulatory effect of indole-3-acetic acid on aerial mycelium formation and antibiotic production in Streptomyces spp. Actinomycetologica. 21:32–39. doi: 10.3209/saj.SAJ210105
   Google Scholar
• Naseem H, Bano A. 2014. Role of plant growth-promoting rhizobacteria and their exopolysaccharide in drought tolerance of maize. J Plant Interact. 9:689–701. doi: 10.1080/17429145.2014.902125
   Web of Science ®Google Scholar
• Ndeddy Aka RJ, Babalola OO. 2016. Effect of bacterial inoculation of strains of Pseudomonas aeruginosa, Alcaligenes feacalis and Bacillus subtilis on germination, growth and heavy metal (Cd, Cr, and Ni) uptake of Brassica juncea. Int J Phytorem. 18:200–209. doi: 10.1080/15226514.2015.1073671
   PubMed Web of Science ®Google Scholar
• Ndeddy Aka RJ, Babalola OO. 2017. Identification and characterization of Cr-, Cd- and Ni- tolerant bacteria isolated from mine tailings. Biorem J. 21:1–19. doi:10.1080/10889868.2017.1282933.
   Web of Science ®Google Scholar
• Ng L, Sariah M, Sariam O, Radziah O, Zainal Abidin M. 2012. Rice seed bacterization for promoting germination and seedling growth under aerobic cultivation system. Austr J Crop Sci. 6:170–175.
   Google Scholar
• Omar M, Osman M, Kasim W, El-Daim IA. 2009. Improvement of salt tolerance mechanisms of barley cultivated under salt stress using Azospirillum brasilense. In: Ashraf M, Ozturk M, Habib-ur-Rehman A, editors. Salinity and water stress improving crop efficiency. Netherlands: Springer. p. 133–147.
   Google Scholar
• Passari AK, Mishra VK, Saikia R, Gupta VK, Singh BP. 2015. Isolation, abundance and phylogenetic affiliation of endophytic actinomycetes associated with medicinal plants and screening for their in vitro antimicrobial biosynthetic potential. Front Microbiol. 6:273. doi:10.3389/fmicb.2015.00273.
   PubMed Web of Science ®Google Scholar
• Patten CL, Glick BR. 2002. Regulation of indoleacetic acid production in Pseudomonas putida GR12-2 by tryptophan and the stationary-phase sigma factor RpoS. Can J Microbiol. 48:635–642. doi: 10.1139/w02-053
   PubMed Web of Science ®Google Scholar
• Penrose DM, Glick BR. 2003. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plant. 118:10–15. doi: 10.1034/j.1399-3054.2003.00086.x
   PubMed Web of Science ®Google Scholar
• Pereyra M, Garcia P, Colabelli M, Barassi C, Creus C. 2012. A better water status in wheat seedlings induced by Azospirillum under osmotic stress is related to morphological changes in xylem vessels of the coleoptile. Appl Soil Ecol. 53:94–97. doi: 10.1016/j.apsoil.2011.11.007
   Web of Science ®Google Scholar
• Philippot L, Raaijmakers JM, Lemanceau P, Van Der Putten WH. 2013. Going back to the roots: the microbial ecology of the rhizosphere. Nat Rev Microbiol. 11:789–799. doi: 10.1038/nrmicro3109
   PubMed Web of Science ®Google Scholar
• Quan C, Wang X, Fan S. 2010. Antifungal compounds of plant growth promoting rhizobacteria and its action mode. In: Maheshwari DK, editor. Plant growth and health promoting bacteria. Berlin-Heidelberg: Springer, Verlag. p. 117–156.
   Google Scholar
• Rashid S, Charles TC, Glick BR. 2012. Isolation and characterization of new plant growth-promoting bacterial endophytes. Appl Soil Ecol. 61:217–224. doi: 10.1016/j.apsoil.2011.09.011
   Web of Science ®Google Scholar
• Rashid M, Khalil S, Ayub N, Alam S, Latif F. 2004. Organic acids production and phosphate solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pak J Biol Sci. 7:187–196. doi: 10.3923/pjbs.2004.187.196
   Google Scholar
• Reddy AR, Chaitanya KV, Vivekanandan M. 2004. Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol. 161:1189–1202. doi: 10.1016/j.jplph.2004.01.013
   PubMed Web of Science ®Google Scholar
• Renn, AL. 2013. Bioinformatic and functional analysis of 1-aminocyclopropane-1-carboxylate (ACC) deaminase homologues in strains of sinorhizobium. Minnesota: Hamline University. Departmental Honors Projects. 4. https://digitalcommons.hamline.edu/dhp/4
   Google Scholar
• Richardson AE. 2001. Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants. Funct Plant Biol. 28:897–906. doi: 10.1071/PP01093
   Web of Science ®Google Scholar
• Rincón A, Valladares F, Gimeno TE, Pueyo JJ. 2008. Water stress responses of two Mediterranean tree species influenced by native soil microorganisms and inoculation with a plant growth promoting rhizobacterium. Tree Physiol. 28:1693–1701. doi: 10.1093/treephys/28.11.1693
   PubMed Web of Science ®Google Scholar
• Rodríguez H, Fraga R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol Adv. 17:319–339. doi: 10.1016/S0734-9750(99)00014-2
   PubMed Web of Science ®Google Scholar
• SAS. 2014. SAS 9.4 output delivery system: user guide. Cary (NC): SAS institute.
   Google Scholar
• Sathya A, Vijayabharathi R, Gopalakrishnan S. 2017. Plant growth-promoting actinobacteria: a new strategy for enhancing sustainable production and protection of grain legumes. 3 Biotechnol. 7:102–112. Springer-Verlag Berlin, Heidelberg.
   PubMedGoogle Scholar
• Schwyn B, Neilands J. 1987. Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 160:47–56. doi: 10.1016/0003-2697(87)90612-9
   PubMed Web of Science ®Google Scholar
• Selvakumar G, Mohan M, Kundu S, Gupta A, Joshi P, Nazim S, Gupta H. 2008. Cold tolerance and plant growth promotion potential of Serratia marcescens strain SRM (MTCC 8708) isolated from flowers of summer squash (Cucurbita pepo). Lett Appl Microbiol. 46:171–175. doi: 10.1111/j.1472-765X.2007.02282.x
   PubMed Web of Science ®Google Scholar
• Shakir MA, Asghari B, Muhammad A. 2012. Rhizosphere bacteria containing ACC-deaminase conferred drought tolerance in wheat grown under semi-arid climate. Soil Environ. 31:108–112.
   Google Scholar
• Sreevidya M, Gopalakrishnan S, Kudapa H, Varshney R. 2016. Exploring plant growth-promotion actinomycetes from vermicompost and rhizosphere soil for yield enhancement in chickpea. Braz J Microbiol. 47:85–95. doi: 10.1016/j.bjm.2015.11.030
   PubMed Web of Science ®Google Scholar
• Taiz L, Zeiger E. 2002. Plant physiology. 5th ed. Massachusetts: Sinauer Associates, Inc.
   Google Scholar
• Vijayabharathi R, Sathya A, Gopalakrishnan S. 2016. A Renaissance in plant growth-promoting and biocontrol agents by endophytes. In: Singh Dhananjaya P., Singh Harikesh P., Prabha Ratna, editors. Microbial inoculants in sustainable agricultural productivity. New Delhi: Springer; p. 37–60. doi:10.1007/978-81-322-2647-5.
   Google Scholar
• Vurukonda SSKP, Vardharajula S, Shrivastava M, SkZ A. 2016. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiol Res. 184:13–24. doi: 10.1016/j.micres.2015.12.003
   PubMed Web of Science ®Google Scholar
• Wahid A, Gelani S, Ashraf M, Foolad MR. 2007. Heat tolerance in plants: an overview. Environ Exp Bot. 61:199–223. doi: 10.1016/j.envexpbot.2007.05.011
   Web of Science ®Google Scholar
• Wang Z, Huang B. 2004. Physiological recovery of Kentucky bluegrass from simultaneous drought and heat stress. Crop Sci. 44:1729–1736. doi: 10.2135/cropsci2004.1729
   Web of Science ®Google Scholar
• Wang C-J, Yang W, Wang C, Gu C, Niu D-D, Liu H-X, Wang Y-P, Guo J-H. 2012. Induction of drought tolerance in cucumber plants by a consortium of three plant growth-promoting rhizobacterium strains. PLoS One. 7:e52565–e52577. doi: 10.1371/journal.pone.0052565
   PubMed Web of Science ®Google Scholar
• Williams P, Phillips G. 2004. Effect of hydrocolloids on emulsion stability, In Gums and Stabilizer’s for the food industry. J Agric Food Chem. 53:3594–4040.
   Google Scholar
• Yandigeri MS, Meena KK, Singh D, Malviya N, Singh DP, Solanki MK, Yadav AK, Arora DK. 2012. Drought-tolerant endophytic actinobacteria promote growth of wheat (Triticum aestivum) under water stress conditions. Plant Growth Regul. 68:411–420. doi: 10.1007/s10725-012-9730-2
   Web of Science ®Google Scholar
• Yang J, Kloepper JW, Ryu C-M. 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci. 14:1–4. doi: 10.1016/j.tplants.2008.10.004
   PubMed Web of Science ®Google Scholar
• Zahid M. 2015. Isolation and identification of indigenous plant growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and nutrient contents of maize (Zea mays L.). Front Microbiol. 6:207. doi: 10.3389/fmicb.2015.00207
   PubMed Web of Science ®Google Scholar
• Zhang H, Kim M-S, Sun Y, Dowd SE, Shi H, Paré PW. 2008. Soil bacteria confer plant salt tolerance by tissue-specific regulation of the sodium transporter HKT1. Mol Plant- Microbe Interact. 21:737–744. doi: 10.1094/MPMI-21-6-0737
   PubMed Web of Science ®Google Scholar