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mAbs Volume 10, 2018 - Issue 4
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Automated high throughput microscale antibody purification workflows for accelerating antibody discovery

Peng LuanDepartment of Antibody Engineering Genentech Inc., South San Francisco, CA, USA
,
Sophia LeeDepartment of Antibody Engineering Genentech Inc., South San Francisco, CA, USA
,
Tia A. ArenaDepartment of Early Stage Cell Culture Genentech Inc., South San Francisco, CA, USAORCID Iconhttp://orcid.org/0000-0002-4283-6223
ORCID Icon,
Maciej PaluchDepartment of Protein Chemistry Genentech Inc., South San Francisco, CA, USA
,
Joe KansoponDepartment of Antibody Engineering Genentech Inc., South San Francisco, CA, USA
,
Sharon ViajarDepartment of Antibody Engineering Genentech Inc., South San Francisco, CA, USA
,
Zahira BegumPurification Development Genentech Inc., South San Francisco, CA, USA
,
Nancy ChiangDepartment of Antibody Engineering Genentech Inc., South San Francisco, CA, USA
,
Gerald NakamuraDepartment of Antibody Engineering Genentech Inc., South San Francisco, CA, USA
,
Philip E. HassDepartment of Protein Chemistry Genentech Inc., South San Francisco, CA, USA
,
Athena W. WongDepartment of Early Stage Cell Culture Genentech Inc., South San Francisco, CA, USA
,
Greg A. LazarDepartment of Antibody Engineering Genentech Inc., South San Francisco, CA, USA
&
Avinash GillDepartment of Antibody Engineering Genentech Inc., South San Francisco, CA, USACorrespondence[email protected]
ORCID Iconhttp://orcid.org/0000-0001-5057-6575
ORCID Icon show all
Pages 624-635 | Received 17 Oct 2017, Accepted 20 Feb 2018, Published online: 29 Mar 2018

References

  • Reichert JM. Antibodies to watch in 2017. MAbs. 2017;9:167–81. doi:10.1080/19420862.2016.1269580. PMID:27960628.
  • Ecker DM, Jones SD, Levine HL. The therapeutic monoclonal antibody market. MAbs. 2015;7:9–14. doi:10.4161/19420862.2015.989042. PMID:25529996.
  • Bos AB, Duque JN, Bhakta S, Farahi F, Chirdon LA, Junutula JR, Harms PD, Wong AW. Development of a semi-automated high throughput transient transfection system. J Biotechnol. 2014;180:10–6. doi:10.1016/j.jbiotec.2014.03.027. PMID:24704608.
  • Bos AB, Luan P, Duque JN, Reilly D, Harms PD, Wong AW. Optimization and automation of an end-to-end high throughput microscale transient protein production process. Biotechnol Bioeng. 2015;112:1832–42. doi:10.1002/bit.25601. PMID:25851051.
  • Grodzki AC, Berenstein E. Antibody purification: Affinity Chromatography – Protein A and Protein G Sepharose. In Oliver C, Jamur MC, eds. Immunocytochemical Methods and Protocols. Totowa, NJ: Humana Press, 2010:33–41.
  • Chhatre S, Titchener-Hooker NJ. Review: Microscale methods for high-throughput chromatography development in the pharmaceutical industry. J Chem Technol Biotechnol. 2009;84:927–40. doi:10.1002/jctb.2125.
  • Schmidt PM, Abdo M, Butcher RE, Yap M-Y, Scotney PD, Ramunno ML, Martin-Roussety G, Owczarek C, Hardy MP, Chen CG, et al. A robust robotic high-throughput antibody purification platform. J Chromatogr A. 2016;1455:9–19. doi:10.1016/j.chroma.2016.05.076. PMID:27283099.
  • Spooner J, Wilkinson T, Kemp BP. Current advances in the development of high-throughput purification strategies for the generation of therapeutic antibodies. Pharm Bioprocess. 2015 3:411–24. doi:10.4155/pbp.15.23.
  • Wang L, Dembecki J, Jaffe N, O'Mara B, Cai H, Sparks C, Zhang J, Laino SG, Russell RJ, Wang M. A safe, effective, and facility compatible cleaning in place procedure for affinity resin in large-scale monoclonal antibody purification. J Chromatogr A. 2013;1308:86–95. doi:10.1016/j.chroma.2013.07.096. PMID:23953712.
  • Yang L, Harding JD, Ivanov AV, Ramasubramanyan N, Dong DD. Effect of cleaning agents and additives on Protein A ligand degradation and chromatography performance. J Chromatogr A. 2015;1385:63–8. doi:10.1016/j.chroma.2015.01.068. PMID:25680549
  • Arora S, Saxena V, Ayyar BV. Affinity chromatography: A versatile technique for antibody purification. Methods. 2017;116:84–94. doi:10.1016/j.ymeth.2016.12.010. PMID:28012937.
  • Ey PL, Prowse SJ, Jenkin CR. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose. Immunochemistry. 1978;15:429–36. doi:10.1016/0161-5890(78)90070-6. PMID:30693.
  • Chapman T. Lab automation and robotics: Automation on the move. Nature. 2003;421:661–6. PMID:12571603.
  • DiMasi JA, Grabowski HG, Hansen RW. Innovation in the pharmaceutical industry: New estimates of R&D costs. J Health Econ. 2016;47:20–33. doi:10.1016/j.jhealeco.2016.01.012. PMID:26928437.

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