The transplantation of the gut microbiome of fat-1 mice protects against colonic mucus layer disruption and endoplasmic reticulum stress induced by high fat diet

References

• Pereira-Lancha LO, Campos-Ferraz PL, Lancha AH Jr. Obesity: considerations about etiology, metabolism, and the use of experimental models. Diabetes Metab Syndr Obes. 2012;5:75–26. doi:10.2147/DMSO.S25026.
   PubMedGoogle Scholar
• Maury E, Brichard SM. Adipokine dysregulation, adipose tissue inflammation and metabolic syndrome. Mol Cell Endocrinol. 2010;314(1):1–16. doi:10.1016/j.mce.2009.07.031.
   PubMed Web of Science ®Google Scholar
• Bidu C, Escoula Q, Bellenger S, Spor A, Galan M, Geissler A, Bouchot A, Dardevet D, Morio-Liondor B, Cani PD, et al. The transplantation of ω3 PUFA–altered gut microbiota of fat-1 mice to wild-type littermates prevents obesity and associated metabolic disorders. Diabetes. 2018;67(8):1512–1523. doi:10.2337/db17-1488.
   PubMed Web of Science ®Google Scholar
• Cani PD, Amar J, Iglesias MA, Poggi M, Knauf C, Bastelica D, Neyrinck AM, Fava F, Tuohy KM, Chabo C, et al. Metabolic endotoxemia initiates obesity and insulin resistance. Diabetes. 2007;56(7):1761–1772. doi:10.2337/db06-1491.
   PubMed Web of Science ®Google Scholar
• Shoelson SE, Lee J, Goldfine AB. Inflammation and insulin resistance. J Clin Invest. 2006;116(7):1793–1801. doi:10.1172/JCI29069.
   PubMed Web of Science ®Google Scholar
• Cani PD, Bibiloni R, Knauf C, Waget A, Neyrinck AM, Delzenne NM, and Burcelin R. Changes in gut microbiota control metabolic endotoxemia-induced inflammation in high-fat diet–induced obesity and diabetes in mice. Diabetes. 2008;57(6):1470–1481. doi:10.2337/db07-1403.
   PubMed Web of Science ®Google Scholar
• Amar J, Burcelin R, Ruidavets JB, Cani PD, Fauvel J, Alessi MC, Chamontin B, Ferrieres J. Energy intake is associated with endotoxemia in apparently healthy men. Am J Clin Nutr. 2008;87(5):1219–1223. doi:10.1093/ajcn/87.5.1219.
   PubMed Web of Science ®Google Scholar
• Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, Ley RE, Sogin ML, Jones WJ, Roe BA, Affourtit JP, et al. A core gut microbiome in obese and lean twins. Nature. 2009;457(7228):480–484. doi:10.1038/nature07540.
   PubMed Web of Science ®Google Scholar
• Murphy EF, Clarke SF, Marques TM, Hill C, Stanton C, Ross RP, O’Doherty RM, Shanahan F, Cotter PD. Antimicrobials: Strategies for targeting obesity and metabolic health? Gut Microbes. 2013;4(1):48–53. doi:10.4161/gmic.22328.
   PubMed Web of Science ®Google Scholar
• Larsen N, Vogensen FK, van den Berg FW, Nielsen DS, Andreasen AS, Pedersen BK, Al-Soud WA, Sorensen SJ, Hansen LH, Jakobsen M, et al. Gut microbiota in human adults with type 2 diabetes differs from non-diabetic adults. PLOS ONE. 2010;5(2):e9085. doi:10.1371/journal.pone.0009085.
   PubMed Web of Science ®Google Scholar
• Qin J, Li Y, Cai Z, Li S, Zhu J, Zhang F, Liang S, Zhang W, Guan Y, Shen D, et al. A metagenome-wide association study of gut microbiota in type 2 diabetes. Nature. 2012;490(7418):55–60. doi:10.1038/nature11450.
   PubMed Web of Science ®Google Scholar
• Paone P, Cani PD. Mucus barrier, mucins and gut microbiota: the expected slimy partners? Gut. 2020;69(12):2232–2243. doi:10.1136/gutjnl-2020-322260.
   PubMed Web of Science ®Google Scholar
• Schroeder BO. Fight them or feed them: how the intestinal mucus layer manages the gut microbiota. Gastroenterol Rep. 2019;7(1):3–12. doi:10.1093/gastro/goy052.
   Google Scholar
• Johansson ME, Larsson JM, Hansson GC. The two mucus layers of colon are organized by the MUC2 mucin, whereas the outer layer is a legislator of host–microbial interactions. Proc Natl Acad Sci USA. 2011;108(Suppl 1):4659–4665. doi:10.1073/pnas.1006451107.
   PubMed Web of Science ®Google Scholar
• Pelaseyed T, Bergstrom JH, Gustafsson JK, Ermund A, Birchenough GM, Schutte A, van der Post S, Svensson F, Rodriguez-Pineiro AM, Nystrom EE, et al. The mucus and mucins of the goblet cells and enterocytes provide the first defense line of the gastrointestinal tract and interact with the immune system. Immunol Rev. 2014;260(1):8–20. doi:10.1111/imr.12182.
   PubMed Web of Science ®Google Scholar
• Schroeder BO, Birchenough GMH, Pradhan M, Nyström EEL, Henricsson M, Hansson GC, Bäckhed F. Obesity-associated microbiota contributes to mucus layer defects in genetically obese mice. J Biol Chem. 2020;295(46):15712–15726. doi:10.1074/jbc.RA120.015771.
   PubMed Web of Science ®Google Scholar
• Hasnain SZ, Tauro S, Das I, Tong H, Chen AC, Jeffery PL, McDonald V, Florin TH, McGuckin MA. IL-10 promotes production of intestinal mucus by suppressing protein misfolding and endoplasmic reticulum stress in goblet cells. Gastroenterology. 2013;144(2):357–368.e359. doi:10.1053/j.gastro.2012.10.043.
   PubMed Web of Science ®Google Scholar
• Gulhane M, Murray L, Lourie R, Tong H, Sheng YH, Wang R, Kang A, Schreiber V, Wong KY, Magor G, et al. High fat diets induce colonic epithelial cell stress and inflammation that is reversed by IL-22. Sci Rep. 2016;6(1):28990. doi:10.1038/srep28990.
   PubMed Web of Science ®Google Scholar
• Yilmaz E. Endoplasmic reticulum stress and obesity. Adv Exp Med Biol. 2017;960:261–276.
   PubMed Web of Science ®Google Scholar
• Ramos-Lopez O, Riezu-Boj JI, Milagro FI, Moreno-Aliaga MJ, Martinez JA. Endoplasmic reticulum stress epigenetics is related to adiposity, dyslipidemia, and insulin resistance. Adipocyte. 2018;7:137–142. doi:10.1080/21623945.2018.1447731.
   PubMed Web of Science ®Google Scholar
• Ozcan U, Cao Q, Yilmaz E, Lee AH, Iwakoshi NN, Ozdelen E, Tuncman G, Gorgun C, Glimcher LH, Hotamisligil GS. Endoplasmic reticulum stress links obesity, insulin action, and type 2 diabetes. Science. 2004;306(5695):457–461. doi:10.1126/science.1103160.
   PubMed Web of Science ®Google Scholar
• Salvado L, Palomer X, Barroso E, Vazquez-Carrera M. Targeting endoplasmic reticulum stress in insulin resistance. Trends Endocrinol Metab. 2015;26(8):438–448. doi:10.1016/j.tem.2015.05.007.
   PubMed Web of Science ®Google Scholar
• Green CD, Olson LK. Modulation of palmitate-induced endoplasmic reticulum stress and apoptosis in pancreatic β-cells by stearoyl-CoA desaturase and Elovl6. Am J Physiol Endocrinol Metab. 2011;300(4):E640–649. doi:10.1152/ajpendo.00544.2010.
   PubMed Web of Science ®Google Scholar
• Peng G, Li L, Liu Y, Pu J, Zhang S, Yu J, Zhao J, Liu P. Oleate blocks palmitate-induced abnormal lipid distribution, endoplasmic reticulum expansion and stress, and insulin resistance in skeletal muscle. Endocrinology. 2011;152(6):2206–2218. doi:10.1210/en.2010-1369.
   PubMed Web of Science ®Google Scholar
• Pfaffenbach KT, Gentile CL, Nivala AM, Wang D, Wei Y, Pagliassotti MJ. Linking endoplasmic reticulum stress to cell death in hepatocytes: roles of C/EBP homologous protein and chemical chaperones in palmitate-mediated cell death. Am J Physiol Endocrinol Metab. 2010;298(5):E1027–1035. doi:10.1152/ajpendo.00642.2009.
   PubMed Web of Science ®Google Scholar
• Escoula Q, Bellenger S, Narce M, Bellenger J. Docosahexaenoic and eicosapentaenoic acids prevent altered-muc2 secretion induced by palmitic acid by alleviating endoplasmic reticulum stress in ls174t goblet cells. Nutrients. 2019;11(9):2179. doi:10.3390/nu11092179.
   PubMed Web of Science ®Google Scholar
• Bellenger J, Bellenger S, Bataille A, Massey KA, Nicolaou A, Rialland M, Tessier C, Kang JX, Narce M. High pancreatic n-3 fatty acids prevent STZ-induced diabetes in fat-1 mice: inflammatory pathway inhibition. Diabetes. 2011;60(4):1090–1099. doi:10.2337/db10-0901.
   PubMed Web of Science ®Google Scholar
• Allegretti JR, Kassam Z, Mullish BH, Chiang A, Carrellas M, Hurtado J, Marchesi JR, McDonald JAK, Pechlivanis A, Barker GF, et al. Effects of fecal microbiota transplantation with oral capsules in obese patients. Clin Gastroenterol Hepatol. 2019;18(4):855–863.e2. doi:10.1016/j.cgh.2019.07.006.
   PubMed Web of Science ®Google Scholar
• Katsoulieris E, Mabley JG, Samai M, Green IC, Chatterjee PK. α-Linolenic acid protects renal cells against palmitic acid lipotoxicity via inhibition of endoplasmic reticulum stress. Eur J Pharmacol. 2009;623(1–3):107–112. doi:10.1016/j.ejphar.2009.09.015.
   PubMed Web of Science ®Google Scholar
• Snodgrass RG, Huang S, Namgaladze D, Jandali O, Shao T, Sama S, Brune B, Hwang DH. Docosahexaenoic acid and palmitic acid reciprocally modulate monocyte activation in part through endoplasmic reticulum stress. J Nutr Biochem. 2016;32:39–45. doi:10.1016/j.jnutbio.2016.01.010.
   PubMed Web of Science ®Google Scholar
• Zhang Y, Dong L, Yang X, Shi H, Zhang L. α-Linolenic acid prevents endoplasmic reticulum stress-mediated apoptosis of stearic acid lipotoxicity on primary rat hepatocytes. Lipids Health Dis. 2011;10(1):81. doi:10.1186/1476-511X-10-81.
   PubMedGoogle Scholar
• Bidu C, Escoula Q, Bellenger S, Spor A, Galan M, Geissler A, Bouchot A, Dardevet D, Morio B, Cani PD, et al. The transplantation of ω3 PUFA–altered gut microbiota of fat-1 mice to wild-type littermates prevents obesity and associated metabolic disorders. Diabetes. 2018;67(8):1512–1523. doi:10.2337/db17-1488.
   PubMed Web of Science ®Google Scholar
• Kang JX, Wang J, Wu L, Kang ZB. Transgenic mice: fat-1 mice convert n-6 to n-3 fatty acids. Nature. 2004;427(6974):504–504. doi:10.1038/427504a.
   PubMed Web of Science ®Google Scholar
• Desai MS, Seekatz AM, Koropatkin NM, Kamada N, Hickey CA, Wolter M, Pudlo NA, Kitamoto S, Terrapon N, Muller A, et al. A dietary fiber-deprived gut microbiota degrades the colonic mucus barrier and enhances pathogen susceptibility. Cell. 2016;167(5):1339–1353.e1321. doi:10.1016/j.cell.2016.10.043.
   PubMed Web of Science ®Google Scholar
• Everard A, Belzer C, Geurts L, Ouwerkerk JP, Druart C, Bindels LB, Guiot Y, Derrien M, Muccioli GG, Delzenne NM, et al. Cross-talk between akkermansia muciniphila and intestinal epithelium controls diet-induced obesity. Proceedings of the National Academy of Sciences of the United States of America; 2013;110:9066–9071. doi:10.1073/pnas.1219451110.
   Google Scholar
• Bel S, Pendse M, Wang Y, Li Y, Ruhn KA, Hassell B, Leal T, Winter SE, Xavier RJ, Hooper LV. Paneth cells secrete lysozyme via secretory autophagy during bacterial infection of the intestine. Science. 2017;357(6355):1047–1052. doi:10.1126/science.aal4677.
   PubMed Web of Science ®Google Scholar
• Patel KK, Miyoshi H, Beatty WL, Head RD, Malvin NP, Cadwell K, Guan JL, Saitoh T, Akira S, Seglen PO, et al. Autophagy proteins control goblet cell function by potentiating reactive oxygen species production. Embo J. 2013;32(24):3130–3144. doi:10.1038/emboj.2013.233.
   PubMed Web of Science ®Google Scholar
• Cornick S, Kumar M, Moreau F, Gaisano H, Chadee K. VAMP8-mediated MUC2 mucin exocytosis from colonic goblet cells maintains innate intestinal homeostasis. Nat Commun. 2019;10(1):4306. doi:10.1038/s41467-019-11811-8.
   PubMedGoogle Scholar
• Mocanu V, Zhang Z, Deehan EC, Kao DH, Hotte N, Karmali S, Birch DW, Samarasinghe KK, Walter J, Madsen KL. Fecal microbial transplantation and fiber supplementation in patients with severe obesity and metabolic syndrome: a randomized double-blind, placebo-controlled phase 2 trial. Nat Med. 2021;27(7):1272–1279. doi:10.1038/s41591-021-01399-2.
   PubMed Web of Science ®Google Scholar
• Ruiz-Núñez B, Pruimboom L, Dijck-Brouwer DA, Muskiet FA. Lifestyle and nutritional imbalances associated with Western diseases: causes and consequences of chronic systemic low-grade inflammation in an evolutionary context. J Nutr Biochem. 2013;24(7):1183–1201. doi:10.1016/j.jnutbio.2013.02.009.
   PubMed Web of Science ®Google Scholar
• Riva A, Kuzyk O, Forsberg E, Siuzdak G, Pfann C, Herbold C, Daims H, Loy A, Warth B, Berry D. A fiber-deprived diet disturbs the fine-scale spatial architecture of the murine colon microbiome. Nat Commun. 2019;10(1):4366. doi:10.1038/s41467-019-12413-0.
   PubMedGoogle Scholar
• Kim KA, Gu W, Lee IA, Joh EH, Kim DH, Chamaillard M. High fat diet-induced gut microbiota exacerbates inflammation and obesity in mice via the TLR4 signaling pathway. PLOS ONE. 2012;7(10):e47713. doi:10.1371/journal.pone.0047713.
   PubMed Web of Science ®Google Scholar
• Han P, Yu Y, Zhang L, Ruan Z. Citrus peel ameliorates mucus barrier damage in HFD-fed mice. J Nutr Biochem. 2022;112:109206. doi:10.1016/j.jnutbio.2022.109206.
   PubMed Web of Science ®Google Scholar
• Liu HY, Walden TB, Ahl D, Nyman M, Bertilsson S, Phillipson M, Holm L. High-fat diet enriched with bilberry modifies colonic mucus dynamics and restores marked alterations of gut microbiome in rats. Mol Nutr Food Res. 2019;63(20):e1900117. doi:10.1002/mnfr.201900117.
   PubMed Web of Science ®Google Scholar
• Atuma C, Strugala V, Allen A, Holm L. The adherent gastrointestinal mucus gel layer: thickness and physical state in vivo. Am J Physiol Gastrointest Liver Physiol. 2001;280(5):G922–929. doi:10.1152/ajpgi.2001.280.5.G922.
   PubMed Web of Science ®Google Scholar
• Święch E, Tuśnio A, Barszcz M, Taciak M, Siwiak E. Goblet cells and mucus layer in the gut of young pigs: Response to dietary contents of threonine and non-essential amino acids. J Anim Physiol Anim Nutr (Berl). 2019;103(3):894–905. doi:10.1111/jpn.13086.
   PubMed Web of Science ®Google Scholar
• Cornick S, Tawiah A, Chadee K. Roles and regulation of the mucus barrier in the gut. Tissue Barriers. 2015;3(1–2):e982426. doi:10.4161/21688370.2014.982426.
   PubMedGoogle Scholar
• Johansson ME, Phillipson M, Petersson J, Velcich A, Holm L, Hansson GC. The inner of the two Muc2 mucin-dependent mucus layers in colon is devoid of bacteria. Proceedings of the National Academy of Sciences of the United States of America; 2008;105:15064–15069. doi:10.1073/pnas.0803124105.
   Google Scholar
• Godl K, Johansson ME, Lidell ME, Mörgelin M, Karlsson H, Olson FJ, Gum JR Jr., Kim YS, Hansson GC. The N terminus of the MUC2 mucin forms trimers that are held together within a trypsin-resistant core fragment. J Biol Chem. 2002;277(49):47248–47256. doi:10.1074/jbc.M208483200.
   PubMed Web of Science ®Google Scholar
• Round AN, Rigby NM, Garcia de la Torre A, Macierzanka A, Mills EN, Mackie AR. Lamellar structures of MUC2-rich mucin: a potential role in governing the barrier and lubricating functions of intestinal mucus. Biomacromolecules. 2012;13(10):3253–3261. doi:10.1021/bm301024x.
   PubMed Web of Science ®Google Scholar
• Cani PD. Human gut microbiome: hopes, threats and promises. Gut. 2018;67(9):1716–1725. doi:10.1136/gutjnl-2018-316723.
   PubMed Web of Science ®Google Scholar
• Schroeder BO, Birchenough GMH, Ståhlman M, Arike L, Johansson MEV, Hansson GC, Bäckhed F. Bifidobacteria or fiber protects against diet-induced microbiota-mediated colonic mucus deterioration. Cell Host & Microbe. 2018;23(1):27–40.e7. doi:10.1016/j.chom.2017.11.004.
   PubMed Web of Science ®Google Scholar
• Birchenough G, Schroeder BO, Bäckhed F, Hansson GC. Dietary destabilisation of the balance between the microbiota and the colonic mucus barrier. Gut Microbes. 2019;10(2):246–250. doi:10.1080/19490976.2018.1513765.
   PubMed Web of Science ®Google Scholar
• Rasheva VI, Domingos PM. Cellular responses to endoplasmic reticulum stress and apoptosis. Apoptosis: Int J Programmed Cell Death. 2009;14(8):996–1007. doi:10.1007/s10495-009-0341-y.
   PubMed Web of Science ®Google Scholar
• Huang D, Xiong M, Xu X, Wu X, Xu J, Cai X, Lu L, Zhou H. Bile acids elevated by high-fat feeding induce endoplasmic reticulum stress in intestinal stem cells and contribute to mucosal barrier damage. Biochem Bioph Res Co. 2020;529(2):289–295. doi:10.1016/j.bbrc.2020.05.226.
   PubMed Web of Science ®Google Scholar
• Tsuboi K, Nishitani M, Takakura A, Imai Y, Komatsu M, Kawashima H. Autophagy protects against colitis by the maintenance of normal gut microflora and secretion of mucus. J Biol Chem. 2015;290(33):20511–20526. doi:10.1074/jbc.M114.632257.
   PubMed Web of Science ®Google Scholar
• Oh H, Cho W, Abd El-Aty AM, Bayram C, Jeong JH, Jung TW. Resolvin D3 improves the impairment of insulin signaling in skeletal muscle and nonalcoholic fatty liver disease through AMPK/autophagy-associated attenuation of ER stress. Biochem Pharmacol. 2022;203:115203. doi:10.1016/j.bcp.2022.115203.
   PubMed Web of Science ®Google Scholar
• Chen Y, Xu C, Yan T, Yu C, Li Y. ω-3 Fatty acids reverse lipotoxity through induction of autophagy in nonalcoholic fatty liver disease. Nutrition (Burbank, Los Angeles County, Calif). 2015;31(11–12):1423–1429.e1422. doi:10.1016/j.nut.2015.05.022.
   PubMed Web of Science ®Google Scholar
• Bae SJ, Kim JE, Choi HJ, Choi YJ, Lee SJ, Gong JE, Seo S, Yang SY, An BS, Lee HS, et al. α-Linolenic acid-enriched cold-pressed perilla oil suppress high-fat diet-induced hepatic steatosis through amelioration of the ER stress-mediated autophagy. Molecules. 2020;25(11):2662. doi:10.3390/molecules25112662.
   PubMed Web of Science ®Google Scholar
• Johansson ME, Jakobsson HE, Holmen-Larsson J, Schutte A, Ermund A, Rodriguez-Pineiro AM, Arike L, Wising C, Svensson F, Backhed F, et al. Normalization of host intestinal mucus layers requires long-term microbial colonization. Cell Host & Microbe. 2015;18(5):582–592. doi:10.1016/j.chom.2015.10.007.
   PubMed Web of Science ®Google Scholar
• Jakobsson HE, Rodríguez-Piñeiro AM, Schütte A, Ermund A, Boysen P, Bemark M, Sommer F, Bäckhed F, Hansson GC, Johansson ME. The composition of the gut microbiota shapes the colon mucus barrier. EMBO Rep. 2015;16(2):164–177. doi:10.15252/embr.201439263.
   PubMed Web of Science ®Google Scholar
• Manichanh C, Reeder J, Gibert P, Varela E, Llopis M, Antolin M, Guigo R, Knight R, Guarner F. Reshaping the gut microbiome with bacterial transplantation and antibiotic intake. Genome Res. 2010;20(10):1411–1419. doi:10.1101/gr.107987.110.
   PubMed Web of Science ®Google Scholar
• Kennedy EA, King KY, Baldridge MT. Mouse microbiota models: comparing germ-free mice and antibiotics treatment as tools for modifying gut bacteria. Front Physiol. 2018;9:1534. doi:10.3389/fphys.2018.01534.
   PubMed Web of Science ®Google Scholar
• Gao X, Chang S, Liu S, Peng L, Xie J, Dong W, Tian Y, Sheng J, Byndloss MX. Correlations between α-linolenic acid-improved multitissue homeostasis and gut microbiota in mice fed a high-fat diet. mSystems. 2020;5(6). doi:10.1128/mSystems.00391-20.
   Web of Science ®Google Scholar
• Natividad JM, Lamas B, Pham HP, Michel ML, Rainteau D, Bridonneau C, da Costa G, van Hylckama Vlieg J, Sovran B, Chamignon C, et al. Bilophila wadsworthia aggravates high fat diet induced metabolic dysfunctions in mice. Nat Commun. 2018;9(1):2802. doi:10.1038/s41467-018-05249-7.
   PubMed Web of Science ®Google Scholar
• Hirano A, Umeno J, Okamoto Y, Shibata H, Ogura Y, Moriyama T, Torisu T, Fujioka S, Fuyuno Y, Kawarabayasi Y, et al. Comparison of the microbial community structure between inflamed and non-inflamed sites in patients with ulcerative colitis. J Gastroen Hepatol. 2018;33(9):1590–1597. doi:10.1111/jgh.14129.
   Web of Science ®Google Scholar
• Thompson RS, Gaffney M, Hopkins S, Kelley T, Gonzalez A, Bowers SJ, Vitaterna MH, Turek FW, Foxx CL, Lowry CA, et al. Ruminiclostridium 5, parabacteroides distasonis, and bile acid profile are modulated by prebiotic diet and associate with facilitated sleep/clock realignment after chronic disruption of rhythms. Brain Behav Immun. 2021;97:150–166. doi:10.1016/j.bbi.2021.07.006.
   PubMed Web of Science ®Google Scholar
• Song H, Shen X, Chu Q, Zheng X. Vaccinium bracteatum thunb. fruit extract reduces high-fat diet-induced obesity with modulation of the gut microbiota in obese mice. J Food Biochem. 2021;45(7):e13808. doi:10.1111/jfbc.13808.
   PubMed Web of Science ®Google Scholar
• Xiao L, Chen B, Feng D, Yang T, Li T, Chen J. TLR4 may be involved in the regulation of colonic mucosal microbiota by vitamin A. Front Microbiol. 2019;10:268. doi:10.3389/fmicb.2019.00268.
   PubMed Web of Science ®Google Scholar
• Wang P, Li D, Ke W, Liang D, Hu X, Chen F. Resveratrol-induced gut microbiota reduces obesity in high-fat diet-fed mice. Int J Obes. 2020;44(1):213–225. doi:10.1038/s41366-019-0332-1.
   PubMed Web of Science ®Google Scholar
• Burger-van Paassen N, Vincent A, Puiman PJ, van der Sluis M, Bouma J, Boehm G, van Goudoever JB, van Seuningen I, Renes IB. The regulation of intestinal mucin MUC2 expression by short-chain fatty acids: implications for epithelial protection. Biochem J. 2009;420(2):211–219. doi:10.1042/BJ20082222.
   PubMed Web of Science ®Google Scholar
• Cândido FG, Valente FX, Grześkowiak ŁM, Moreira APB, Rocha D, Alfenas RCG. Impact of dietary fat on gut microbiota and low-grade systemic inflammation: mechanisms and clinical implications on obesity. Int J Food Sci Nutr. 2018;69(2):125–143. doi:10.1080/09637486.2017.1343286.
   PubMed Web of Science ®Google Scholar
• Desbois AP, Smith VJ. Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Appl Microbiol Biotechnol. 2010;85(6):1629–1642. doi:10.1007/s00253-009-2355-3.
   PubMed Web of Science ®Google Scholar
• Kaliannan K, Wang B, Li XY, Kim KJ, Kang JX. A host-microbiome interaction mediates the opposing effects of omega-6 and omega-3 fatty acids on metabolic endotoxemia. Sci Rep. 2015;5(1):11276. doi:10.1038/srep11276.
   PubMedGoogle Scholar
• Arike L, Hansson GC. The densely O-Glycosylated MUC2 mucin protects the intestine and provides food for the commensal bacteria. J Mol Biol. 2016;428(16):3221–3229. doi:10.1016/j.jmb.2016.02.010.
   PubMed Web of Science ®Google Scholar
• Etienne-Mesmin L, Chassaing B, Desvaux M, De Paepe K, Gresse R, Sauvaitre T, Forano E, de Wiele TV, Schüller S, Juge N, et al. Experimental models to study intestinal microbes–mucus interactions in health and disease. FEMS Microbiology Reviews. 2019;43(5):457–489. doi:10.1093/femsre/fuz013.
   PubMed Web of Science ®Google Scholar
• Schroeder BO, Birchenough GMH, Stahlman M, Arike L, Johansson MEV, Hansson GC, Backhed F. Bifidobacteria or fiber protects against diet-induced microbiota-mediated colonic mucus deterioration. Cell Host & Microbe. 2018;23(1):27–40.e7. doi:10.1016/j.chom.2017.11.004.
   PubMed Web of Science ®Google Scholar
• Herath M, Hosie S, Bornstein JC, Franks AE, Hill-Yardin EL. The role of the gastrointestinal mucus system in intestinal homeostasis: implications for neurological disorders. Front Cell Infect Microbiol. 2020;10:248. doi:10.3389/fcimb.2020.00248.
   PubMed Web of Science ®Google Scholar
• Orsso CE, Colin-Ramirez E, Field CJ, Madsen KL, Prado CM, Haqq AM. Adipose tissue development and expansion from the womb to adolescence: an overview. Nutrients. 2020;12(9):2735. doi:10.3390/nu12092735.
   PubMed Web of Science ®Google Scholar
• Novelle MG, Diéguez C. Updating gender differences in the control of homeostatic and hedonic food intake: Implications for binge eating disorder. Mol Cell Endocrinol. 2019;497:110508. doi:10.1016/j.mce.2019.110508.
   PubMed Web of Science ®Google Scholar
• Pettersson US, Waldén TB, Carlsson PO, Jansson L, Phillipson M, Maedler K. Female mice are protected against high-fat diet induced metabolic syndrome and increase the regulatory T cell population in adipose tissue. PLOS ONE. 2012;7(9):e46057. doi:10.1371/journal.pone.0046057.
   PubMed Web of Science ®Google Scholar
• Asarian L, Geary N. Modulation of appetite by gonadal steroid hormones. Philos Trans Roy Soc London. 2006;361(1471):1251–1263. doi:10.1098/rstb.2006.1860.
   PubMed Web of Science ®Google Scholar
• Markle JG, Frank DN, Mortin-Toth S, Robertson CE, Feazel LM, Rolle-Kampczyk U, von Bergen M, McCoy KD, Macpherson AJ, Danska JS. Sex differences in the gut microbiome drive hormone-dependent regulation of autoimmunity. Science. 2013;339(6123):1084–1088. doi:10.1126/science.1233521.
   PubMed Web of Science ®Google Scholar
• Haro C, Rangel-Zúñiga OA, Alcalá-Díaz JF, Gómez-Delgado F, Pérez-Martínez P, Delgado-Lista J, Quintana-Navarro GM, Landa BB, Navas-Cortés JA, Tena-Sempere M, et al. Intestinal microbiota is influenced by gender and body mass index. PLOS ONE. 2016;11(5):e0154090. doi:10.1371/journal.pone.0154090.
   PubMed Web of Science ®Google Scholar
• Bolnick DI, Snowberg LK, Hirsch PE, Lauber CL, Org E, Parks B, Lusis AJ, Knight R, Caporaso JG, Svanbäck R. Individual diet has sex-dependent effects on vertebrate gut microbiota. Nat Commun. 2014;5(1):4500. doi:10.1038/ncomms5500.
   PubMedGoogle Scholar
• Morissette A, Kropp C, Songpadith JP, Junges Moreira R, Costa J, Mariné-Casadó R, Pilon G, Varin TV, Dudonné S, Boutekrabt L, et al. Blueberry proanthocyanidins and anthocyanins improve metabolic health through a gut microbiota-dependent mechanism in diet-induced obese mice. Am J Physiol Endocrinol Metab. 2020;318(6):E965–e980. doi:10.1152/ajpendo.00560.2019.
   PubMed Web of Science ®Google Scholar
• Aron-Wisnewsky J, Warmbrunn MV, Nieuwdorp M, Clément K. Metabolism and metabolic disorders and the microbiome: the intestinal microbiota associated with obesity, lipid metabolism, and metabolic health—pathophysiology and therapeutic strategies. Gastroenterology. 2021;160(2):573–599. doi:10.1053/j.gastro.2020.10.057.
   PubMed Web of Science ®Google Scholar
• Bojanova DP, Bordenstein SR. Fecal transplants: what is being transferred? PLOS Biol. 2016;14(7):e1002503. doi:10.1371/journal.pbio.1002503.
   PubMed Web of Science ®Google Scholar
• Yang AM, Inamine T, Hochrath K, Chen P, Wang L, Llorente C, Bluemel S, Hartmann P, Xu J, Koyama Y. et al. Intestinal fungi contribute to development of alcoholic liver disease. J Clin Invest. 2017;127(7):2829–2841. doi:10.1172/JCI90562.
   PubMed Web of Science ®Google Scholar
• Bajaj JS, Liu EJ, Kheradman R, Fagan A, Heuman DM, White M, Gavis EA, Hylemon P, Sikaroodi M, Gillevet PM. Fungal dysbiosis in cirrhosis. Gut. 2018;67(6):1146–1154. doi:10.1136/gutjnl-2016-313170.
   PubMed Web of Science ®Google Scholar
• Daniel N, Le Barz M, Mitchell PL, Varin TV, Julien IB, Farabos D, Pilon G, Gauthier J, Garofalo C, Kang JX. et al. Comparing transgenic production to supplementation of ω-3 PUFA reveals distinct but overlapping mechanisms underlying protection against metabolic and hepatic disorders. Function (Oxford, England). 2023;4(2):zqac069. doi:10.1093/function/zqac069.
   PubMedGoogle Scholar
• Yu HN, Zhu J, Pan WS, Shen SR, Shan WG, Das UN. Effects of fish oil with a high content of n-3 polyunsaturated fatty acids on mouse gut microbiota. Arch Med Res. 2014;45(3):195–202. doi:10.1016/j.arcmed.2014.03.008.
   PubMed Web of Science ®Google Scholar
• Hakimian JK, Dong TS, Barahona JA, Lagishetty V, Tiwari S, Azani D, Barrera M, Lee S, Severino AL, Mittal N, et al. Dietary supplementation with omega-3 polyunsaturated fatty acids reduces opioid-seeking behaviors and alters the gut microbiome. Nutrients. 2019;11(8):1900. doi:10.3390/nu11081900.
   PubMed Web of Science ®Google Scholar
• Fevre C, Bellenger S, Pierre AS, Minville M, Bellenger J, Gresti J, Rialland M, Narce M, Tessier C. The metabolic cascade leading to eicosanoid precursors–desaturases, elongases, and phospholipases A2–is altered in Zucker fatty rats. Biochimica et Biophysica Acta (BBA) - Mol Cell Biol Lipids. 2011;1811(6):409–417. doi:10.1016/j.bbalip.2010.12.004.
   PubMed Web of Science ®Google Scholar
• Zou Z, Bellenger S, Massey KA, Nicolaou A, Geissler A, Bidu C, Bonnotte B, Pierre AS, Minville-Walz M, Rialland M, et al. Inhibition of the HER2 pathway by n-3 polyunsaturated fatty acids prevents breast cancer in fat-1 transgenic mice. J Lipid Res. 2013;54(12):3453–3463. doi:10.1194/jlr.M042754.
   PubMed Web of Science ®Google Scholar
• Pais de Barros JP, Gautier T, Sali W, Adrie C, Choubley H, Charron E, Lalande C, Le Guern N, Deckert V, Monchi M, et al. Quantitative lipopolysaccharide analysis using liquid chromatography-tandem mass spectrometry and its combination with the limulus amebocyte lysate assay. Journal of Lipid Research. 2015;56(7):1363–1369. doi:10.1194/jlr.D059725.
   PubMedGoogle Scholar
• Achard CS, Dupouy V, Cauquil L, Arpaillange N, Bousquet-Melou A, Floc’h NL, Zemb O. Early inoculation of microbial suspension in suckling piglets affects the transmission of maternal microbiota and the associated antibiotic resistance genes. Microorganisms. 2020;8(10):1576. doi:10.3390/microorganisms8101576.
   PubMed Web of Science ®Google Scholar
• Le Floc’h N, Knudsen C, Gidenne T, Montagne L, Merlot E, Zemb O. Impact of feed restriction on health, digestion and faecal microbiota of growing pigs housed in good or poor hygiene conditions. Anim: Int J Anim Biosci. 2014;8(10):1632–1642. doi:10.1017/S1751731114001608.
   PubMed Web of Science ®Google Scholar
• Verschuren LMG, Calus MPL, Jansman AJM, Bergsma R, Knol EF, Gilbert H, Zemb O. Fecal microbial composition associated with variation in feed efficiency in pigs depends on diet and sex. J Anim Sci. 2018;96(4):1405–1418. doi:10.1093/jas/sky060.
   PubMed Web of Science ®Google Scholar
• Magoč T, Salzberg SL. FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics. 2011;27(21):2957–2963. doi:10.1093/bioinformatics/btr507.
   PubMed Web of Science ®Google Scholar
• Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJ, Holmes SP. DADA2: High-resolution sample inference from illumina amplicon data. Nat Methods. 2016;13(7):581–583. doi:10.1038/nmeth.3869.
   PubMed Web of Science ®Google Scholar
• Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Glöckner FO. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Research. 2013;41(D1):D590–596. doi:10.1093/nar/gks1219.
   PubMed Web of Science ®Google Scholar
• Beaumont M, Paës C, Mussard E, Knudsen C, Cauquil L, Aymard P, Barilly C, Gabinaud B, Zemb O, Fourre S, et al. Gut microbiota derived metabolites contribute to intestinal barrier maturation at the suckling-to-weaning transition. Gut Microbes. 2020;11(5):1268–1286. doi:10.1080/19490976.2020.1747335.
   PubMed Web of Science ®Google Scholar