Efficient isolation and culture of endothelial cells from venous malformation using the Rho-associated protein kinase inhibitor Y27632

References

• Mulliken JB, Glowacki J. Hemangiomas and vascular malformations in infants and children: a classification based on endothelial characteristics. Plast Reconstr Surg 1982;69:412–22.
   PubMed Web of Science ®Google Scholar
• Wang Y, Qi F, Gu J. Endothelial cell culture of intramuscular venous malformation and its invasive behavior related to matrix metalloproteinase-9. Plast Reconstr Surg 2009;123:1419–30.
   PubMed Web of Science ®Google Scholar
• Shireman PK, McCarthy WJ, Yao JS, Vogelzang RL. Treatment of venous malformations by direct injection with ethanol. J Vasc Surg 1997;26:838–44.
   PubMed Web of Science ®Google Scholar
• Vikkula M, Boon LM, Carraway KL, et al. Vascular dysmorphogenesis caused by an activating mutation in the receptor tyrosine kinase TIE2. Cell 1996;87:1181–90.
   PubMed Web of Science ®Google Scholar
• Eerola I, Boon LM, Mulliken JB, et al. Capillary malformation-arteriovenous malformation, a new clinical and genetic disorder caused by RASA1 mutations. Am J Hum Genet 2003;73:1240–9.
   PubMed Web of Science ®Google Scholar
• DeCarlo AA, Cohen JA, Aguado A, Glenn B. Isolation and characterization of human gingival microvascular endothelial cells. J Periodont Res 2008;43:246–54.
   PubMed Web of Science ®Google Scholar
• Jiang M, Min Y, DeBusk L, et al. Spontaneous immortalization of human dermal microvascular endothelial cells. World J Stem Cells 2010;2:114–20.
   PubMedGoogle Scholar
• Terunuma A, Limgala RP, Park CJ, et al. Efficient procurement of epithelial stem cells from human tissue specimens using a Rho-assisted protein kinase inhibitor Y27632. Tissue Eng 2010;16:1363–8.
   Google Scholar
• Yoshimura K, Shigeura T, Matsumoto D, et al. Characterization of freshly isolated and cultured cells derived from the fatty and fluid portions of liposuction aspirates. J Cell Physiol 2006;208:64–76.
   PubMed Web of Science ®Google Scholar
• Chapman S, Liu X, Meyers C, et al. Human keratinocytes are efficiently immortalized by a Rho kinase inhibitor. J Clin Invest 2010;120:2619–26.
   PubMed Web of Science ®Google Scholar
• Oswald J, Boxberger S, Jørgensen B, et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro. Stem Cells 2004;22:377–84.
   PubMed Web of Science ®Google Scholar
• Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest 1973;52:2745–56.
   PubMed Web of Science ®Google Scholar
• Moss SC, Bates M, Parrino PE, Woods TC. Isolation of endothelial cells and vascular smooth muscle cells from internal mammary artery tissue. Ochsner J 2007;7:133–6.
   PubMedGoogle Scholar
• Wautier MP, Boval B, Chappey O, et al. Cultured endothelial cells from human arteriovenous malformations have defective growth regulation. Blood 1999;94:2020–8.
   PubMed Web of Science ®Google Scholar
• Manconi F, Markham R, Fraser IS. Culturing endothelial cells of microvascular origin. Methods Cell Sci 2000;22:89–99.
   PubMedGoogle Scholar
• Joo HJ, Choi DK, Lim JS, et al. ROCK suppression promotes differentiation and expansion of endothelial cells from embryonic stem cell-derived Flk1(+) mesodermal precursor cells. Blood 2012;120:2733–44.
   PubMed Web of Science ®Google Scholar
• Bekhite MM, Finkensieper A, Binas S, et al. VEGF-mediated PI3K class IA and PKC signaling in cardiomyogenesis and vasculogenesis of mouse embryonic stem cells. J Cell Sci 2011;124:1819–30.
   PubMed Web of Science ®Google Scholar
• Pagiatakis C, Gordon JW, Ehyai S, McDermott JC. A novel RhoA/ROCK-CPI-17-MEF2C signaling pathway regulates vascular smooth muscle cell gene expression. J Biol Chem 2012;287:8361–70.
   PubMedGoogle Scholar
• Uehata M, Ishizaki T, Satoh H, et al. Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension. Nature 1997;389:990–4.
   PubMed Web of Science ®Google Scholar
• Sakamoto K, Hori M, Izumi M, et al. Inhibition of high K+-induced contraction by the ROCKs inhibitor Y27632 in vascular smooth muscle: possible involvement of ROCKs in a signal transduction pathway. J Pharmacol Sci 2003;92:56–69.
   PubMed Web of Science ®Google Scholar
• Sawada N, Itoh H, Ueyama K, et al. Inhibition of Rho-associated kinase results in suppression of neointimal formation of balloon-injured arteries. Circulation 2000;101:2030–3.
   PubMed Web of Science ®Google Scholar
• Horwitz A, Parsons J. Cell migration-movin’ on. Science 1999;286:1102–3.
   PubMed Web of Science ®Google Scholar
• Ai S, Kuzuya M, Koike T, et al. Rho-Rho kinase is involved in smooth muscle cell migration through myosin light chain phosphorylation-dependent and independent pathways. Atherosclerosis 2001;155:321–7.
   PubMed Web of Science ®Google Scholar
• Funakoshi Y, Ichiki T, Shimokawa H, et al. Rho-kinase mediates angiotensin II: induced monocyte chemoattractant protein-1 expression in rat vascular smooth muscle cells. Hypertension 2001;38:101–4.
   Web of Science ®Google Scholar
• Matsumoto Y, Uwatoku T, Abe K, et al. Long-term inhibition of Rho-kinase suppresses neointimal formation after stent implantation in porcine coronary arteries: involvement of multiple mechanisms. Arterioscler Thromb Vasc Biol 2004;24:181–6.
   PubMed Web of Science ®Google Scholar
• Watanabe K, Ueno M, Kamiya D, et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol 2007;25:681–6.
   PubMed Web of Science ®Google Scholar
• Siljee S, Keane E, Marsh R, et al. Expression of the components of the renin–angiotensin system in venous malformation. Front Surg 2016;3:24.
   PubMed Web of Science ®Google Scholar
• Balakumar P, Jagadeesh G. Potential cross-talk between (pro)renin receptors and Wnt/frizzled receptors in cardiovascular and renal disorders. Hypertens Res 2011;34:1161–70.
   PubMed Web of Science ®Google Scholar
• Castel P, Carmona J, Grego-Bessa J, Berger MF. Somatic PIK3CA mutations as a driver of sporadic venous malformations. Sci Transl Med 2016;8:332ra42.
   PubMed Web of Science ®Google Scholar
• Castillo SD, Tzouanacou E, Berenjeno IM. Somatic activation mutations in Pik3ca cause sporadic venous malformation in mice and humans. Sci Transl Med 2016;8:332ra43.
   PubMed Web of Science ®Google Scholar
• Limaye N, Kangas J, Godfraind C, et al. Somatic activating PIK3CA mutations cause venous malformation. Am J Hum Genet 2015;97:914–21.
   PubMed Web of Science ®Google Scholar