References
- References, especially those provided to illustrate methods and approaches, are representative only, and are not meant to be a comprehensive review of the literature. Most references were derived from suggestions provided in the MISEV2018 Survey results. Each reference was checked by multiple authors. Citation implies deemed relevance of scientific content and not an endorsement by the authors or ISEV of any particular journal or editorial practice.
- Lotvall J, Hill AF, Hochberg F, et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the international society for extracellular vesicles. J Extracell Vesicles. 2014;3:26913. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25536934
- Witwer KW, Soekmadji C, Hill AF, et al. Updating the MISEV minimal requirements for extracellular vesicle studies: building bridges to reproducibility. J Extracell Vesicles. 2017;6(1):1396823. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1396823
- Stein JM, Luzio JP Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles. Biochem J. 1991;274 (Pt 2):381–43. Available from: http://www.ncbi.nlm.nih.gov/pubmed/1848755
- Cocucci E, Meldolesi J Ectosomes and exosomes: shedding the confusion between extracellular vesicles. Trends Cell Biol. 2015;25(6):364–372. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25683921
- Gould SJ, Raposo G As we wait: coping with an imperfect nomenclature for extracellular vesicles. J Extracell Vesicles. 2013;2. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24009890
- Gardiner C, Di Vizio D, Sahoo S, et al. Techniques used for the isolation and characterization of extracellular vesicles: results of a worldwide survey. J Extracell Vesicles. 2016;5:32945. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27802845
- Rojas A The imperative authentication of cell lines. Antimicrob Agents Chemother. 2017;61(11):e01823–17. Available from: http://aac.asm.org/lookup/doi/10.1128/AAC.01823-17
- Reid Y, Storts D, Riss T, et al. Authentication of human cell lines by STR DNA profiling analysis [Internet]. Assay Guidance Manual. 2004. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23805434
- Chen TS, Arslan F, Yin Y, et al. Enabling a robust scalable manufacturing process for therapeutic exosomes through oncogenic immortalization of human ESC-derived MSCs. J Transl Med. 2011;9(1):47. Available from: http://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-9-47
- Lima LG, Chammas R, Monteiro RQ, et al. Tumor-derived microvesicles modulate the establishment of metastatic melanoma in a phosphatidylserine-dependent manner. Cancer Lett. 2009;283(2):168–175. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0304383509002420
- Frey B, Gaipl US The immune functions of phosphatidylserine in membranes of dying cells and microvesicles. Semin Immunopathol. 2011;33(5):497–516. Available from: http://link.springer.com/10.1007/s00281-010-0228-6
- Roseblade A, Luk F, Ung A, et al. Targeting microparticle biogenesis: a novel approach to the circumvention of cancer multidrug resistance. Curr Cancer Drug Targets. 2015;15(3):205–214. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25714701
- Takasugi M Emerging roles of extracellular vesicles in cellular senescence and aging. Aging Cell. 2018;17(2):e12734.
- Patel DB, Gray KM, Santharam Y, et al. Impact of cell culture parameters on production and vascularization bioactivity of mesenchymal stem cell-derived extracellular vesicles. Bioeng Transl Med. 2017;2(2):170–179.
- Dang VD, Jella KK, Ragheb RRT, et al. Lipidomic and proteomic analysis of exosomes from mouse cortical collecting duct cells. FASEB J. 2017;31(12):5399–5408. Available from: http://www.fasebj.org/doi/10.1096/fj.201700417R
- Klingeborn M, Dismuke WM, Skiba NP, et al. Directional exosome proteomes reflect polarity-specific functions in retinal pigmented epithelium monolayers. Sci Rep. 2017;7(1):4901. Available from: http://www.nature.com/articles/s41598-017-05102-9
- Mittelbrunn M, Vicente-Manzanares M, Sánchez-Madrid F Organizing polarized delivery of exosomes at synapses. Traffic. 2015;16(4):327–337. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25614958
- van Niel G, Raposo G, Candalh C, et al. Intestinal epithelial cells secrete exosome-like vesicles. Gastroenterology. 2001;121(2):337–349. Available from: http://www.ncbi.nlm.nih.gov/pubmed/11487543
- Tauro BJ, Greening DW, Mathias RA, et al. Two distinct populations of exosomes are released from LIM1863 colon carcinoma cell-derived organoids. Mol Cell Proteomics. 2013;12(3):587–598. Available from: http://www.mcponline.org/lookup/doi/10.1074/mcp.M112.021303
- Yan IK, Shukla N, Borrelli DA, et al. Use of a hollow fiber bioreactor to collect extracellular vesicles from cells in culture. Methods Mol Biol. 2018;1740:35–41. Available from: http://link.springer.com/10.1007/978-1-4939-7652-2_4
- Watson DC, Yung BC, Bergamaschi C, et al. Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes. J Extracell Vesicles. 2018;7(1):1442088. Available from: http://www.ncbi.nlm.nih.gov/pubmed/29535850
- Lowry MC, O’Driscoll L Can hi-jacking hypoxia inhibit extracellular vesicles in cancer? Drug Discov Today. 2018;23(6):1267–1273. Available from: https://linkinghub.elsevier.com/retrieve/pii/S1359644617303252
- Mitchell MD, Peiris HN, Kobayashi M, et al. Placental exosomes in normal and complicated pregnancy. Am J Obstet Gynecol. 2015;213(4Suppl): S173–81. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0002937815007176
- de Jong OG, Verhaar MC, Chen Y, et al. Cellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes. J Extracell Vesicles. 2012;1(1):18396. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v1i0.18396
- Stratton D, Moore C, Antwi-Baffour S, et al. Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9. Biochem Biophys Res Commun. 2015;460(3):589–595. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0006291X15005203
- Dozio V, Sanchez J-C Characterisation of extracellular vesicle-subsets derived from brain endothelial cells and analysis of their protein cargo modulation after TNF exposure. J Extracell Vesicles. 2017;6(1):1302705. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1302705
- Taylor J, Jaiswal R, Bebawy M Calcium-calpain dependent pathways regulate vesiculation in malignant breast cells. Curr Cancer Drug Targets. 2017;17(5):486–494. Available from: http://www.eurekaselect.com/node/146745/article
- Mostefai HA, Agouni A, Carusio N, et al. Phosphatidylinositol 3-kinase and xanthine oxidase regulate nitric oxide and reactive oxygen species productions by apoptotic lymphocyte microparticles in endothelial cells. J Immunol. 2008;180(7):5028–5035. Available from: http://www.ncbi.nlm.nih.gov/pubmed/18354228
- Agouni A, Mostefai HA, Porro C, et al. Sonic hedgehog carried by microparticles corrects endothelial injury through nitric oxide release. FASEB J. 2007;21(11):2735–2741. Available from: http://www.fasebj.org/doi/10.1096/fj.07-8079com
- Soekmadji C, Riches JD, Russell PJ, et al. Modulation of paracrine signaling by CD9 positive small extracellular vesicles mediates cellular growth of androgen deprived prostate cancer. Oncotarget. 2017;8(32):52237–52255. Available from: http://www.oncotarget.com/fulltext/11111
- Saari H, Lázaro-Ibáñez E, Viitala T, et al. Microvesicle- and exosome-mediated drug delivery enhances the cytotoxicity of paclitaxel in autologous prostate cancer cells. J Control Release. 2015;220(PtB):727–737. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0168365915301322
- Lázaro-Ibáñez E, Neuvonen M, Takatalo M, et al. Metastatic state of parent cells influences the uptake and functionality of prostate cancer cell-derived extracellular vesicles. J Extracell Vesicles. 2017;6(1):1354645. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1354645
- Chernov VM, Mouzykantov AA, Baranova NB, et al. Extracellular membrane vesicles secreted by mycoplasma acholeplasma laidlawii PG8 are enriched in virulence proteins. J Proteomics. 2014;110:117–128. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1874391914003819
- Corral-Vázquez C, Aguilar-quesada R, Catalina P, et al. Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking. Cell Tissue Bank. 2017;18(2):271–280. Available from: http://link.springer.com/10.1007/s10561-017-9617-6
- Yang C, Chalasani G, Ng Y-H, et al. Exosomes released from mycoplasma infected tumor cells activate inhibitory B cells. PLoS One. 2012;7(4):e36138. Available from: http://dx.plos.org/10.1371/journal.pone.0036138
- Quah BJC, O’Neill HC Mycoplasma contaminants present in exosome preparations induce polyclonal B cell responses. J Leukoc Biol. 2007;82(5):1070–1082.
- Mathivanan S, Lim JW, Tauro BJ, et al. Proteomics analysis of A33 immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature. Mol Cell Proteomics. 2010;9(2):197–208. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19837982
- Burger D, Turner M, Xiao F, et al. High glucose increases the formation and pro-oxidative activity of endothelial microparticles. Diabetologia. 2017;60(9):1791–1800. Available from: http://link.springer.com/10.1007/s00125-017-4331-2
- Thom SR, Bhopale VM, Yu K, et al. Neutrophil microparticle production and inflammasome activation by hyperglycemia due to cytoskeletal instability. J Biol Chem. 2017;292(44):18312–18324. Available from: http://www.jbc.org/lookup/doi/10.1074/jbc.M117.802629
- Rice GE, Scholz-Romero K, Sweeney E, et al. The effect of glucose on the release and bioactivity of exosomes from first trimester trophoblast cells. J Clin Endocrinol Metab. 2015;100(10):E1280–8. Available from: https://academic.oup.com/jcem/article-lookup/doi/10.1210/jc.2015-2270
- Németh A, Orgovan N, Sódar BW, et al. Antibiotic-induced release of small extracellular vesicles (exosomes) with surface-associated DNA. Sci Rep. 2017;7(1):8202. Available from: http://www.nature.com/articles/s41598-017-08392-1
- Zhou X, Zhang W, Yao Q, et al. Exosome production and its regulation of EGFR during wound healing in renal tubular cells. Am J Physiol Renal Physiol. 2017;312(6):F963–70. Available from: http://www.physiology.org/doi/10.1152/ajprenal.00078.2017
- Pachler K, Lener T, Streif D, et al. A good manufacturing practice-grade standard protocol for exclusively human mesenchymal stromal cell-derived extracellular vesicles. Cytotherapy. 2017;19(4):458–472. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1465324917300038
- Saury C, Lardenois A, Schleder C, et al. Human serum and platelet lysate are appropriate xeno-free alternatives for clinical-grade production of human MuStem cell batches. Stem Cell Res Ther. 2018;9(1):128. Available from: https://stemcellres.biomedcentral.com/articles/10.1186/s13287-018-0852-y
- Li J, Lee Y, Johansson HJ, et al. Serum-free culture alters the quantity and protein composition of neuroblastoma-derived extracellular vesicles. J Extracell Vesicles. 2015;4(1):26883. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v4.26883
- Beninson LA, Fleshner M Exosomes in fetal bovine serum dampen primary macrophage IL-1β response to lipopolysaccharide (LPS) challenge. Immunol Lett. 2015;163(2):187–192. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25455591
- Eitan E, Zhang S, Witwer KW, et al. Extracellular vesicle-depleted fetal bovine and human sera have reduced capacity to support cell growth. J Extracell Vesicles. 2015;4:26373. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25819213
- Théry C, Amigorena S, Raposo G, et al. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. In: Current protocols in cell biology. Hoboken, NJ, USA: John Wiley & Sons, Inc.; 2006. p. Unit 3.22. Available from: http://www.ncbi.nlm.nih.gov/pubmed/18228490
- van Balkom BWM, de Jong OG, Smits M, et al. Endothelial cells require miR-214 to secrete exosomes that suppress senescence and induce angiogenesis in human and mouse endothelial cells. Blood. 2013;121(19):3997–4006. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23532734
- Kornilov R, Puhka M, Mannerström B, et al. Efficient ultrafiltration-based protocol to deplete extracellular vesicles from fetal bovine serum. J Extracell Vesicles. 2018;7(1):1422674. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1422674
- Wei Z, Batagov AO, Carter DRF, et al. Fetal bovine serum RNA interferes with the cell culture derived extracellular RNA. Sci Rep. 2016;6:31175. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27503761
- Shelke GV, Lässer C, Gho YS, et al. Importance of exosome depletion protocols to eliminate functional and RNA-containing extracellular vesicles from fetal bovine serum. J Extracell Vesicles. 2014;3:24783. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25317276
- Tosar JP, Cayota A, Eitan E, et al. Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components? J Extracell Vesicles. 2017;6(1):1272832. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28326168
- Kaur S, Singh SP, Elkahloun AG, et al. CD47-dependent immunomodulatory and angiogenic activities of extracellular vesicles produced by T cells. Matrix Biol. 2014;37:49–59. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0945053X14000924
- Witwer KW, Buzas EI, Bemis LT, et al. Standardization of sample collection, isolation and analysis methods in extracellular vesicle research: an ISEV position paper. J Extracell Vesicles. 2013;2:20360.
- Mateescu B, Kowal EJK, van Balkom BWM, Bartel S, Bhattacharyya SN, Buzàs EI, et al. Obstacles and opportunities in the functional analysis of extracellular vesicle RNA- An ISEV Position Paper. J Extracell Vesicles. 2017;6:1286095.
- Bæk R, Søndergaard EKL, Varming K, et al. The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray. J Immunol Meth. 2016;438:11–20. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0022175916301624
- Barteneva NS, Fasler-Kan E, Bernimoulin M, et al. Circulating microparticles: square the circle. BMC Cell Biol. 2013;14(1):23. Available from: http://bmccellbiol.biomedcentral.com/articles/10.1186/1471-2121-14-23
- Mullier F, Bailly N, Chatelain C, et al. Pre-analytical issues in the measurement of circulating microparticles: current recommendations and pending questions. J Thromb Haemost. 2013. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23410207
- Lacroix R, Judicone C, Poncelet P, et al. Impact of pre-analytical parameters on the measurement of circulating microparticles: towards standardization of protocol. J Thromb Haemost. 2012;10(3):437–446. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22212198
- Coumans FAW, Brisson AR, Buzas EI, et al. Methodological guidelines to study extracellular vesicles. Circ Res. 2017;120(10):1632–1648. Available from: http://circres.ahajournals.org/lookup/doi/10.1161/CIRCRESAHA.117.309417
- Yuana Y, Bertina RM, Osanto S Pre-analytical and analytical issues in the analysis of blood microparticles. Thromb Haemost. 2011;105(3):396–408. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21174005
- Yuana Y, Böing AN, Grootemaat AE, et al. Handling and storage of human body fluids for analysis of extracellular vesicles. J Extracell Vesicles. 2015;4:29260.
- Robbins PD Extracellular vesicles and aging. Stem Cell Investig. 2017;4(12):98. Available from: http://sci.amegroups.com/article/view/17758/18069
- Danielson KM, Estanislau J, Tigges J, et al. Diurnal variations of circulating extracellular vesicles measured by nano flow cytometry. PLoS One. 2016;11(1):e0144678. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26745887
- Fendl B, Weiss R, Fischer MB, et al. Characterization of extracellular vesicles in whole blood: influence of pre-analytical parameters and visualization of vesicle-cell interactions using imaging flow cytometry. Biochem Biophys Res Commun. 2016;478(1):168–173. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0006291X16311950
- Wisgrill L, Lamm C, Hartmann J, et al. Peripheral blood microvesicles secretion is influenced by storage time, temperature, and anticoagulants. Cytometry A. 2016;89(7):663–672.
- György B, Pálóczi K, Kovács A, et al. Improved circulating microparticle analysis in acid-citrate dextrose (ACD) anticoagulant tube. Thromb Res. 2014;133(2):285–292. Available from: http://linkinghub.elsevier.com/retrieve/pii/S004938481300546X
- Mitchell AJ, Gray WD, Hayek SS, et al. Platelets confound the measurement of extracellular miRNA in archived plasma. Sci Rep. 2016;6(1):32651. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27623086
- Cheng HH, Yi HS, Kim Y, et al. Plasma processing conditions substantially influence circulating microRNA biomarker levels. PLoS One. 2013;8(6):e64795. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23762257
- Muller L, Hong C-S, Stolz DB, et al. Isolation of biologically-active exosomes from human plasma. J Immunol Meth. 2014;411:55–65.
- Ayers L, Kohler M, Harrison P, et al. Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. Thromb Res. 2011;127(4):370–377. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21257195
- Heijnen HF, Schiel AE, Fijnheer R, et al. Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules. Blood. 1999;94(11):3791–3799.
- Mincheva-Nilsson L, Baranov V, Nagaeva O, et al. Isolation and characterization of exosomes from cultures of tissue explants and cell lines. Curr Protoc Immunol. 2016;115:14.42.1–14.42.21.
- Lunavat TR, Cheng L, Einarsdottir BO, et al. BRAFV600 inhibition alters the microRNA cargo in the vesicular secretome of malignant melanoma cells. Proc Natl Acad Sci U S A. 2017;114(29):E5930–9. Available from: http://www.pnas.org/lookup/doi/10.1073/pnas.1705206114
- Gupta AK, Rusterholz C, Huppertz B, et al. A comparative study of the effect of three different syncytiotrophoblast micro-particles preparations on endothelial cells. Placenta. 2005;26(1):59–66. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0143400404001080
- Holder BS, Tower CL, Forbes K, et al. Immune cell activation by trophoblast-derived microvesicles is mediated by syncytin 1. Immunology. 2012;136(2):184–191.
- Perez-Gonzalez R, Gauthier SA, Kumar A, et al. The exosome secretory pathway transports amyloid precursor protein carboxyl-terminal fragments from the cell into the brain extracellular space. J Biol Chem. 2012;287(51):43108–43115. Available from: http://www.jbc.org/lookup/doi/10.1074/jbc.M112.404467
- Vella LJ, Scicluna BJ, Cheng L, et al. A rigorous method to enrich for exosomes from brain tissue. J Extracell Vesicles. 2017;6(1):1348885. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28804598
- Deng ZB, Poliakov A, Hardy RW, et al. Adipose tissue exosome-like vesicles mediate activation of macrophage-induced insulin resistance. Diabetes. 2009;58(11):2498–2505. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19675137
- Wang GJ, Liu Y, Qin A, et al. Thymus exosomes-like particles induce regulatory T cells. J Immunol. 2008;181(8):5242–5248. Available from: http://www.ncbi.nlm.nih.gov/pubmed/18832678
- Kranendonk MEG, Visseren FLJ, van Balkom BWM, et al. Human adipocyte extracellular vesicles in reciprocal signaling between adipocytes and macrophages. Obesity (Silver Spring). 2014;22(5):1296–1308.
- Loyer X, Zlatanova I, Devue C, et al. Intra-cardiac release of extracellular vesicles shapes inflammation following myocardial infarction. Circ Res. 2018;123(1):100–106. Available from: http://circres.ahajournals.org/lookup/doi/10.1161/CIRCRESAHA.117.311326
- Leroyer AS, Ebrahimian TG, Cochain C, et al. Microparticles from ischemic muscle promotes postnatal vasculogenesis. Circulation. 2009;119(21):2808–2817. Available from: http://circ.ahajournals.org/cgi/doi/10.1161/CIRCULATIONAHA.108.816710
- Michaelis ML, Jiang L, Michaelis EK Isolation of synaptosomes, synaptic plasma membranes, and synaptic junctional complexes. In: Methods in molecular biology. Clifton, NJ. 2017. p. 107–119. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27943187
- Zhou H, Yuen PS, Pisitkun T, et al. Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney Int. 2006;69(8):1471–1476. Available from: http://www.ncbi.nlm.nih.gov/pubmed/16501490
- Vila-Liante V, Sánchez-López V, Martínez-Sales V, et al. Impact of sample processing on the measurement of circulating microparticles: storage and centrifugation parameters. Clin Chem Lab Med. 2016;54(11):1759–1767. Available from: https://www.degruyter.com/view/j/cclm.2016.54.issue-11/cclm-2016-0036/cclm-2016-0036.xml
- Kriebardis AG, Antonelou MH, Georgatzakou HT, et al. Microparticles variability in fresh frozen plasma: preparation protocol and storage time effects. Blood Transfus. 2016;14(2):228–237. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27136430
- Lőrincz ÁM, Timár CI, Marosvári KA, et al. Effect of storage on physical and functional properties of extracellular vesicles derived from neutrophilic granulocytes. J Extracell Vesicles. 2014;3(1):25465. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v3.25465
- Bosch S, de Beaurepaire L, Allard M, et al. Trehalose prevents aggregation of exosomes and cryodamage. Sci Rep. 2016;6(1):36162. Available from: http://www.nature.com/articles/srep36162
- Maroto R, Zhao Y, Jamaluddin M, et al. Effects of storage temperature on airway exosome integrity for diagnostic and functional analyses. J Extracell Vesicles. 2017;6(1):1359478. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1359478
- Jin Y, Chen K, Wang Z, et al. DNA in serum extracellular vesicles is stable under different storage conditions. BMC Cancer. 2016;16(1):753. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27662833
- Jeyaram A, Jay SM. Preservation and storage stability of extracellular vesicles for therapeutic applications. Aaps J. 2017;20(1):1. Available from: http://link.springer.com/10.1208/s12248-017-0160-y
- Trummer A, De Rop C, Tiede A, et al. Recovery and composition of microparticles after snap-freezing depends on thawing temperature. Blood Coagul Fibrinolysis. 2009;20(1):52–56. Available from: https://insights.ovid.com/crossref?an=00001721-200901000-00010
- Lener T, Gimona M, Aigner L, et al. Applying extracellular vesicles based therapeutics in clinical trials - an ISEV position paper. J Extracell Vesicles. 2015;4:30087. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=4698466&tool=pmcentrez&rendertype=abstract
- Reiner AT, Witwer KW, Van Balkom BWM, et al. Concise review: developing best-practice models for the therapeutic use of extracellular vesicles. Stem Cells Transl Med. 2017;6(8).
- Clayton A, Buschmann D, Byrd JB, et al. Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017. J Extracell Vesicles. 2018;7(1):1473707. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1473707
- Montis C, Zendrini A, Valle F, et al. Size distribution of extracellular vesicles by optical correlation techniques. Colloids Surf B Biointerfaces. 2017;158:331–338. Available from: http://linkinghub.elsevier.com/retrieve/pii/S092777651730406X
- Morales-Kastresana A, Telford B, Musich TA, et al. Labeling extracellular vesicles for nanoscale flow cytometry. Sci Rep. 2017;7(1):1878. Available from: http://www.nature.com/articles/s41598-017-01731-2
- Corso G, Mäger I, Lee Y, et al. Reproducible and scalable purification of extracellular vesicles using combined bind-elute and size exclusion chromatography. Sci Rep. 2017;7(1):11561. Available from: http://www.nature.com/articles/s41598-017-10646-x
- Welton JL, Webber JP, Botos L-A, et al. Ready-made chromatography columns for extracellular vesicle isolation from plasma. J Extracell Vesicles. 2015;4:27269. Available from: http://www.tandfonline.com/doi/full/10.3402/jev.v4.27269
- Vergauwen G, Dhondt B, Van Deun J, et al. Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research. Sci Rep. 2017;7(1):2704. Available from: http://www.nature.com/articles/s41598-017-02599-y
- Lobb RJ, Becker M, Wen SW, et al. Optimized exosome isolation protocol for cell culture supernatant and human plasma. J Extracell Vesicles. 2015;4:27031. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v4.27031
- Tan CY, Lai RC, Wong W, et al. Mesenchymal stem cell-derived exosomes promote hepatic regeneration in drug-induced liver injury models. Stem Cell Res Ther. 2014;5(3):76. Available from: http://stemcellres.com/content/5/3/76
- Jong AY, Wu C-H, Li J, et al. Large-scale isolation and cytotoxicity of extracellular vesicles derived from activated human natural killer cells. J Extracell Vesicles. 2017;6(1):1294368. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1294368
- Heinemann ML, Ilmer M, Silva LP, et al. Benchtop isolation and characterization of functional exosomes by sequential filtration. J Chromatogr A. 2014;1371:125–135. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0021967314015908
- Heinemann ML, Vykoukal J Sequential filtration: A gentle method for the isolation of functional extracellular vesicles. In: Methods in molecular biology. Clifton, NJ. 2017. p. 33–41. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28828646
- Wei Z, Batagov AO, Schinelli S, et al. Coding and noncoding landscape of extracellular RNA released by human glioma stem cells. Nat Commun. 2017;8(1):1145. Available from: http://www.nature.com/articles/s41467-017-01196-x
- Lamparski HG, Metha-Damani A, Yao JY, et al. Production and characterization of clinical grade exosomes derived from dendritic cells. J Immunol Meth. 2002;270(2):211–226. Available from: http://www.ncbi.nlm.nih.gov/pubmed/12379326
- Escudier B, Dorval T, Chaput N, et al. Vaccination of metastatic melanoma patients with autologous dendritic cell (DC) derived-exosomes: results of thefirst phase I clinical trial. J Transl Med. 2005;3(1):10. Available from: http://www.ncbi.nlm.nih.gov/pubmed/15740633
- Roda B, Zattoni A, Reschiglian P, et al. Field-flow fractionation in bioanalysis: A review of recent trends. Anal Chim Acta. 2009;635(2):132–143. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0003267009000865
- Zhang H, Freitas D, Kim HS, et al. Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation. Nat Cell Biol. 2018;20(3):332–343. Available from: http://www.ncbi.nlm.nih.gov/pubmed/29459780
- Yang JS, Lee JC, Byeon SK, et al. Size dependent lipidomic analysis of urinary exosomes from patients with prostate cancer by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry. Anal Chem. 2017;89(4):2488–2496. Available from: http://pubs.acs.org/doi/10.1021/acs.analchem.6b04634
- Agarwal K, Saji M, Lazaroff SM, et al. Analysis of exosome release as a cellular response to MAPK pathway inhibition. Langmuir. 2015;31(19):5440–5448. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25915504
- Liu C, Guo J, Tian F, et al. Field-free isolation of exosomes from extracellular vesicles by microfluidic viscoelastic flows. ACS Nano. 2017;11(7):6968–6976. Available from: http://pubs.acs.org/doi/10.1021/acsnano.7b02277
- Ibsen SD, Wright J, Lewis JM, et al. Rapid isolation and detection of exosomes and associated biomarkers from plasma. ACS Nano. 2017;11(7):6641–6651. Available from: http://pubs.acs.org/doi/10.1021/acsnano.7b00549
- Lewis JM, Vyas AD, Qiu Y, et al. Integrated analysis of exosomal protein biomarkers on alternating current electrokinetic chips enables rapid detection of pancreatic cancer in patient blood. ACS Nano. 2018;12(4):3311–3320. Available from: http://pubs.acs.org/doi/10.1021/acsnano.7b08199
- Lee K, Shao H, Weissleder R, et al. Acoustic purification of extracellular microvesicles. ACS Nano. 2015;9(3):2321–2327. Available from: http://pubs.acs.org/doi/10.1021/nn506538f
- Satzer P, Wellhoefer M, Jungbauer A. Continuous separation of protein loaded nanoparticles by simulated moving bed chromatography. J Chromatogr A. 2014;1349:44–49. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0021967314006979
- Mol EA, Goumans M-J, Doevendans PA, et al. Higher functionality of extracellular vesicles isolated using size-exclusion chromatography compared to ultracentrifugation. Nanomedicine. 2017;13(6):2061–2065. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1549963417300540
- de Menezes-Neto A, Sáez MJF, Lozano-Ramos I, et al. Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. J Extracell Vesicles. 2015;4:27378. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26154623
- Kosanović M, Milutinović B, Goč S, et al. Ion-exchange chromatography purification of extracellular vesicles. Biotechniques. 2017;63(2):65–71. Available from: https://www.future-science.com/doi/10.2144/000114575
- Heath N, Grant L, De Oliveira TM, et al. Rapid isolation and enrichment of extracellular vesicle preparations using anion exchange chromatography. Sci Rep. 2018;8(1):5730. Available from: http://www.ncbi.nlm.nih.gov/pubmed/29636530
- Kim D, Nishida H, An SY, et al. Chromatographically isolated CD63 + CD81 + extracellular vesicles from mesenchymal stromal cells rescue cognitive impairments after TBI. Proc Natl Acad Sci. 2016;113(1):170–175. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26699510
- Merchant ML, Powell DW, Wilkey DW, et al. Microfiltration isolation of human urinary exosomes for characterization by MS. PROTEOMICS - Clin Appl. 2010;4(1):84–96. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21137018
- Higginbotham JN, Zhang Q, Jeppesen DK, et al. Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting. J Extracell Vesicles. 2016;5:29254. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27345057
- Groot Kormelink T, Arkesteijn GJA, Nauwelaers FA, et al. Prerequisites for the analysis and sorting of extracellular vesicle subpopulations by high-resolution flow cytometry. Cytometry A. 2016;89(2):135–147. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25688721
- Atkin-Smith GK, Paone S, Zanker DJ, et al. Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting. Sci Rep. 2017;7:39846. Available from: http://www.nature.com/articles/srep39846
- Minciacchi VR, Spinelli C, Reis-Sobreiro M, et al. MYC mediates large oncosome-induced fibroblast reprogramming in prostate cancer. Cancer Res. 2017;77(9):2306–2317. Available from: http://cancerres.aacrjournals.org/lookup/doi/10.1158/0008-5472.CAN-16-2942
- Wunsch BH, Smith JT, Gifford SM, et al. Nanoscale lateral displacement arrays for the separation of exosomes and colloids down to 20 nm. Nat Nanotechnol. 2016;11(11):936–940. Available from: http://www.nature.com/articles/nnano.2016.134
- Echevarria J, Royo F, Pazos R, et al. Microarray-based identification of lectins for the purification of human urinary extracellular vesicles directly from urine samples. Chembiochem. 2014;15(11):1621–1626.
- Ghosh A, Davey M, Chute IC, et al. Rapid isolation of extracellular vesicles from cell culture and biological fluids using a synthetic peptide with specific affinity for heat shock proteins. PLoS One. 2014;9(10):e110443. Available from: http://dx.plos.org/10.1371/journal.pone.0110443
- Balaj L, Atai NA, Chen W, et al. Heparin affinity purification of extracellular vesicles. Sci Rep. 2015;5:10266. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25988257
- Fang X, Duan Y, Adkins GB, et al. Highly efficient exosome isolation and protein analysis by an integrated nanomaterial-based platform. Anal Chem. 2018;90(4):2787–2795. Available from: http://pubs.acs.org/doi/10.1021/acs.analchem.7b04861
- Sharma P, Ludwig S, Muller L, et al. Immunoaffinity-based isolation of melanoma cell-derived exosomes from plasma of patients with melanoma. J Extracell Vesicles. 2018;7(1):1435138. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1435138
- Brett SI, Lucien F, Guo C, et al. Immunoaffinity based methods are superior to kits for purification of prostate derived extracellular vesicles from plasma samples. Prostate. 2017;77(13):1335–1343.
- Nakai W, Yoshida T, Diez D, et al. A novel affinity-based method for the isolation of highly purified extracellular vesicles. Sci Rep. 2016;6(1):33935. Available from: http://www.nature.com/articles/srep33935
- Welton JL, Loveless S, Stone T, et al. Cerebrospinal fluid extracellular vesicle enrichment for protein biomarker discovery in neurological disease; multiple sclerosis. J Extracell Vesicles. 2017;6(1):1369805. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1369805
- Lai RC, Tan SS, Yeo RWY, et al. MSC secretes at least 3 EV types each with a unique permutation of membrane lipid, protein and RNA. J Extracell Vesicles. 2016;5(1):29828. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v5.29828
- Gallart-Palau X, Serra A, Wong ASW, et al. Extracellular vesicles are rapidly purified from human plasma by PRotein Organic Solvent PRecipitation (PROSPR). Sci Rep. 2015;5(1):14664. Available from: http://www.nature.com/articles/srep14664
- Shin H, Han C, Labuz JM, et al. High-yield isolation of extracellular vesicles using aqueous two-phase system. Sci Rep. 2015;5(1):13103. Available from: http://www.nature.com/articles/srep13103
- Hurwitz SN, Nkosi D, Conlon MM, et al. CD63 regulates epstein-barr virus LMP1 exosomal packaging, enhancement of vesicle production, and noncanonical NF-κB signaling. J Virol. 2017;91(5):e02251–16. Available from: http://jvi.asm.org/lookup/doi/10.1128/JVI.02251-16
- Musante L, Tataruch D, Gu D, et al. A simplified method to recover urinary vesicles for clinical applications, and sample banking. Sci Rep. 2014;4(1):7532. Available from: http://www.nature.com/articles/srep07532
- Sedykh SE, Purvinish LV, Monogarov AS, et al. Purified horse milk exosomes contain an unpredictable small number of major proteins. Biochim Open. 2017;4:61–72. Available from: http://linkinghub.elsevier.com/retrieve/pii/S2214008517300056
- Contreras-Naranjo JC, Wu H-J, Ugaz VM Microfluidics for exosome isolation and analysis: enabling liquid biopsy for personalized medicine. Lab Chip. 2017;17(21):3558–3577.
- Wu M, Ouyang Y, Wang Z, et al. Isolation of exosomes from whole blood by integrating acoustics and microfluidics. Proc Natl Acad Sci U S A. 2017;114(40):10584–10589. Available from: http://www.pnas.org/lookup/doi/10.1073/pnas.1709210114
- Chen C, Skog J, Hsu CH, et al. Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip. 2010/02/04. 2010;10(4):505–511.
- Liang L-G, Kong M-Q, Zhou S, et al. An integrated double-filtration microfluidic device for isolation, enrichment and quantification of urinary extracellular vesicles for detection of bladder cancer. Sci Rep. 2017;7:46224. Available from: http://www.nature.com/articles/srep46224
- Shin S, Han D, Park MC, et al. Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems. Sci Rep. 2017;7(1):9907. Available from: http://www.nature.com/articles/s41598-017-08826-w
- Yasui T, Yanagida T, Ito S, et al. Unveiling massive numbers of cancer-related urinary-microRNA candidates via nanowires. Sci Adv. 2017;3(12):e1701133. Available from: http://advances.sciencemag.org/lookup/doi/10.1126/sciadv.1701133
- Zhao Z, Yang Y, Zeng Y, et al. A microfluidic exosearch chip for multiplexed exosome detection towards blood-based ovarian cancer diagnosis. Lab Chip. 2016;16(3):489–496.
- Wang Z, Wu H, Fine D, et al. Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles. Lab Chip. 2013;13(15):2879–2882.
- Reátegui E, van der Vos KE, Lai CP, et al. Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles. Nat Commun. 2018;9(1):175. Available from: http://www.nature.com/articles/s41467-017-02261-1
- Böing AN, van der Pol E, Grootemaat AE, et al. Single-step isolation of extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles. 2014;3:23430. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v3.23430
- Stranska R, Gysbrechts L, Wouters J, et al. Comparison of membrane affinity-based method with size-exclusion chromatography for isolation of exosome-like vesicles from human plasma. J Transl Med. 2018;16(1):1. Available from: https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-017-1374-6
- Enderle D, Spiel A, Coticchia CM, et al. Characterization of RNA from exosomes and other extracellular vesicles isolated by a novel spin column-based method. PLoS One. 2015;10(8):e0136133. Available from: http://dx.plos.org/10.1371/journal.pone.0136133
- Jeppesen DK, Hvam ML, Primdahl-Bengtson B, et al. Comparative analysis of discrete exosome fractions obtained by differential centrifugation. J Extracell Vesicles. 2014;3:25011. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25396408
- Livshits MA, Khomyakova E, Evtushenko EG, et al. Isolation of exosomes by differential centrifugation: theoretical analysis of a commonly used protocol. Sci Rep. 2015;5(1):17319. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26616523
- Jang SC, Kim OY, Yoon CM, et al. Bioinspired exosome-mimetic nanovesicles for targeted delivery of chemotherapeutics to malignant tumors. ACS Nano. 2013;7(9):7698–7710. Available from: http://pubs.acs.org/doi/10.1021/nn402232g
- Li K, Wong DK, Hong KY, et al. Cushioned-density gradient ultracentrifugation (C-DGUC): a refined and high performance method for the isolation, characterization, and use of exosomes. Methods Mol Biol. 2018;1740:69–83. Available from: http://link.springer.com/10.1007/978-1-4939-7652-2_7
- Van Deun J, Mestdagh P, Agostinis P, et al. EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research. Nat Methods. 2017;14(3):228–232. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28245209
- Mitchell JP, Court J, Mason MD, et al. Increased exosome production from tumour cell cultures using the integra celline culture system. J Immunol Meth. 2008;335(1–2):98–105. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0022175908000926
- Ortiz A, Sanchez-Niño MD, Sanz AB The meaning of urinary creatinine concentration. Kidney Int. 2011;79(7):791. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0085253815548849
- Cointe S, Judicone C, Robert S, et al. Standardization of microparticle enumeration across different flow cytometry platforms: results of a multicenter collaborative workshop. J Thromb Haemost. 2017;15(1):187–193.
- Krishnan SR, Luk F, Brown RD, et al. Isolation of human CD138(+) microparticles from the plasma of patients with multiple myeloma. Neoplasia. 2016;18(1):25–32. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1476558615001566
- McVey MJ, Spring CM, Semple JW, et al. Microparticles as biomarkers of lung disease: enumeration in biological fluids using lipid bilayer microspheres. Am J Physiol Lung Cell Mol Physiol. 2016;310(9):L802–14. Available from: http://www.physiology.org/doi/10.1152/ajplung.00369.2015
- Atkin-Smith GK, Tixeira R, Paone S, et al. A novel mechanism of generating extracellular vesicles during apoptosis via a beads-on-a-string membrane structure. Nat Commun. 2015;6:7439. Available from: http://www.nature.com/doifinder/10.1038/ncomms8439
- van der Vlist EJ, Nolte-’T Hoen EN, Stoorvogel W, et al. Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry. Nat Protoc. 2012/06/23. 2012;7(7):1311–1326. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22722367
- van der Pol E, van Gemert MJ, Sturk A, et al. Single vs. swarm detection of microparticles and exosomes by flow cytometry. J Thromb Haemost. 2012/03/08. 2012;10(5):919–930. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22394434
- Pospichalova V, Svoboda J, Dave Z, et al. Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer. J Extracell Vesicles. 2015;4(1):25530. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v4.25530
- Tian Y, Ma L, Gong M, et al. Protein profiling and sizing of extracellular vesicles from colorectal cancer patients via flow cytometry. ACS Nano. 2018;12(1):671–680. Available from: http://pubs.acs.org/doi/10.1021/acsnano.7b07782
- McVey MJ, Spring CM, Kuebler WM. Improved resolution in extracellular vesicle populations using 405 instead of 488 nm side scatter. J Extracell Vesicles. 2018;7(1):1454776. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2018.1454776
- Nolan JP, Stoner SA. A trigger channel threshold artifact in nanoparticle analysis. Cytometry A. 2013;83(3):301–305.
- Arraud N, Linares R, Tan S, et al. Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration. J Thromb Haemost. 2014;12(5):614–627.
- Arraud N, Gounou C, Linares R, et al. A simple flow cytometry method improves the detection of phosphatidylserine-exposing extracellular vesicles. J Thromb Haemost. 2015;13(2):237–247.
- Maas SLN, de Vrij J, van der Vlist EJ, et al. Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics. J Control Release. 2015;200:87–96. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0168365914008384
- de Vrij J, Maas SL, van Nispen M, et al. Quantification of nanosized extracellular membrane vesicles with scanning ion occlusion sensing. Nanomedicine (Lond). 2013. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23384702
- Obeid S, Ceroi A, Mourey G, et al. Development of a NanoBioAnalytical platform for on-chip qualification and quantification of platelet-derived microparticles. Biosens Bioelectron. 2017;93:250–259. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0956566316308569
- Libregts SFWM, Arkesteijn GJA, Németh A, et al. Flow cytometric analysis of extracellular vesicle subsets in plasma: impact of swarm by particles of non-interest. J Thromb Haemost. 2018;16(7):1423–1436.
- van der Pol E, Hoekstra AG, Sturk A, et al. Optical and non-optical methods for detection and characterization of microparticles and exosomes. J Thromb Haemost. 2010;8(12):2596–2607. Available from: http://www.ncbi.nlm.nih.gov/pubmed/20880256
- Carnell-Morris P, Tannetta D, Siupa A, et al. Analysis of extracellular vesicles using fluorescence nanoparticle tracking analysis. Methods Mol Biol. 2017;1660:153–173. Available from: http://link.springer.com/10.1007/978-1-4939-7253-1_13
- Takov K, Yellon DM, Davidson SM Confounding factors in vesicle uptake studies using fluorescent lipophilic membrane dyes. J Extracell Vesicles. 2017;6(1):1388731. Available from: http://www.ncbi.nlm.nih.gov/pubmed/29184625
- van der Pol E, Coumans FAW, Grootemaat AE, et al. Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing. J Thromb Haemost. 2014;12(7):1182–1192. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24818656
- Dragovic RA, Gardiner C, Brooks AS, et al. Sizing and phenotyping of cellular vesicles using nanoparticle tracking analysis. Nanomedicine. 2011;7(6):780–788. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21601655
- Gardiner C, Ferreira YJ, Dragovic RA, et al. Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis. J Extracell Vesicles. 2013;2:19671. Available from:
- Osteikoetxea X, Balogh A, Szabó-Taylor K, et al. Improved characterization of EV preparations based on protein to lipid ratio and lipid properties. PLoS One. 2015;10(3):e0121184. Available from: http://dx.plos.org/10.1371/journal.pone.0121184
- Benmoussa A, Ly S, Shan ST, et al. A subset of extracellular vesicles carries the bulk of microRNAs in commercial dairy cow’s milk. J Extracell Vesicles. 2017;6(1):1401897. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1401897
- Mihály J, Deák R, Szigyártó IC, et al. Characterization of extracellular vesicles by IR spectroscopy: fast and simple classification based on amide and CH stretching vibrations. Biochim Biophys Acta. 2017;1859(3):459–466. Available from: http://linkinghub.elsevier.com/retrieve/pii/S000527361630390X
- Turchinovich A, Weiz L, Langheinz A, et al. Characterization of extracellular circulating microRNA. Nucleic Acids Res. 2011;39(16):7223–7233. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21609964
- Arroyo JD, Chevillet JR, Kroh EM, et al. Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci U S A. 2011;108(12):5003–5008. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21383194
- Vickers KC, Palmisano BT, Shoucri BM, et al. MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat Cell Biol. 2011;13(4):423–433. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21423178
- Duijvesz D, Versluis CYL, van der Fels CAM, et al. Immuno-based detection of extracellular vesicles in urine as diagnostic marker for prostate cancer. Int J Cancer. 2015;137(12):2869–2878.
- Suárez H, Gámez-Valero A, Reyes R, et al. A bead-assisted flow cytometry method for the semi-quantitative analysis of extracellular vesicles. Sci Rep. 2017;7(1):11271. Available from: http://www.nature.com/articles/s41598-017-11249-2
- Koliha N, Wiencek Y, Heider U, et al. A novel multiplex bead-based platform highlights the diversity of extracellular vesicles. J Extracell Vesicles. 2016;5:29975. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26901056
- Xia Y, Liu M, Wang L, et al. A visible and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes. Biosens Bioelectron. 2017;92:8–15. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0956566317300635
- Liang K, Liu F, Fan J, et al. Nanoplasmonic quantification of tumor-derived extracellular vesicles in plasma microsamples for diagnosis and treatment monitoring. Nat Biomed Eng. 2017;1(4):0021. Available from: http://www.nature.com/articles/s41551-016-0021
- Rupert DLM, Lässer C, Eldh M, et al. Determination of exosome concentration in solution using surface plasmon resonance spectroscopy. Anal Chem. 2014;86(12):5929–5936. Available from: http://pubs.acs.org/doi/10.1021/ac500931f
- Webber J, Clayton A How pure are your vesicles? J Extracell Vesicles. 2013;2:19861. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24009896
- Maiolo D, Paolini L, Di Noto G, et al. Colorimetric nanoplasmonic assay to determine purity and titrate extracellular vesicles. Anal Chem. 2015;87(8):4168–4176. Available from: http://pubs.acs.org/doi/abs/10.1021/ac504861d
- Lai RC, Arslan F, Lee MM, et al. Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury. Stem Cell Res. 2010;4(3):214–222.
- Cvjetkovic A, Lotvall J, Lasser C The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles. J Extracell Vesicles. 2014;3:23111. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24678386
- Valkonen S, van der Pol E, Böing A, et al. Biological reference materials for extracellular vesicle studies. Eur J Pharm Sci. 2017;98:4–16. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0928098716303578
- Minciacchi VR, You S, Spinelli C, et al. Large oncosomes contain distinct protein cargo and represent a separate functional class of tumor-derived extracellular vesicles. Oncotarget. 2015;6(13):11327–11341. Available from: http://www.oncotarget.com/fulltext/3598
- Keerthikumar S, Gangoda L, Liem M, et al. Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes. Oncotarget. 2015;6(17):15375–15396. Available from: http://www.oncotarget.com/fulltext/3801
- Haraszti RA, Didiot M-C, Sapp E, et al. High-resolution proteomic and lipidomic analysis of exosomes and microvesicles from different cell sources. J Extracell Vesicles. 2016;5(1):32570. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v5.32570
- Clark DJ, Fondrie WE, Liao Z, et al. Redefining the breast cancer exosome proteome by tandem mass tag quantitative proteomics and multivariate cluster analysis. Anal Chem. 2015;87(20):10462–10469. Available from: http://pubs.acs.org/doi/10.1021/acs.analchem.5b02586
- Durcin M, Fleury A, Taillebois E, et al. Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles. J Extracell Vesicles. 2017;6(1):1305677. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1305677
- Kowal J, Arras G, Colombo M, et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci. 2016;113(8):E968–77. Available from: http://www.pnas.org/lookup/doi/10.1073/pnas.1521230113
- Xu R, Greening DW, Rai A, et al. Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. Methods. 2015;87:11–25. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1046202315001541
- Willms E, Johansson HJ, Mäger I, et al. Cells release subpopulations of exosomes with distinct molecular and biological properties. Sci Rep. 2016;6(1):22519. Available from: http://www.nature.com/articles/srep22519
- Meehan B, Rak J, Di Vizio D Oncosomes - large and small: what are they, where they came from? J Extracell Vesicles. 2016;5:33109. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27680302
- Sódar BW, Kittel Á, Pálóczi K, et al. Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection. Sci Rep. 2016;6:24316. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27087061
- Karimi N, Cvjetkovic A, Jang SC, et al. Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins. Cell Mol Life Sci. 2018;75(15):2873–2886. Available from: http://link.springer.com/10.1007/s00018-018-2773-4
- Østergaard O, Nielsen CT, Iversen LV, et al. Quantitative proteome profiling of normal human circulating microparticles. J Proteome Res. 2012;11(4):2154–2163. Available from: http://pubs.acs.org/doi/10.1021/pr200901p
- Musante L, Saraswat M, Duriez E, et al. Biochemical and physical characterisation of urinary nanovesicles following CHAPS treatment. PLoS One. 2012;7(7):e37279. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22808001
- Van Deun J, Mestdagh P, Sormunen R, et al. The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J Extracell Vesicles. 2014;3. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25317274
- McKenzie AJ, Hoshino D, Hong NH, et al. KRAS-MEK Signaling Controls Ago2 Sorting into Exosomes. Cell Rep. 2016;15(5):978–987. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27117408
- Melo SAA, Sugimoto H, O’Connell JT, et al. Cancer exosomes perform cell-independent microRNA biogenesis and promote tumorigenesis. Cancer Cell. 2014;26(5):707–721. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25446899
- Buck AH, Coakley G, Simbari F, et al. Exosomes secreted by nematode parasites transfer small RNAs to mammalian cells and modulate innate immunity. Nat Commun. 2014;5(1):5488. Available from: http://www.nature.com/articles/ncomms6488
- Tkach M, Kowal J, Zucchetti AE, et al. Qualitative differences in T-cell activation by dendritic cell-derived extracellular vesicle subtypes. Embo J. 2017;36(20):3012–3028. Available from: http://emboj.embopress.org/lookup/doi/10.15252/embj.201696003
- Jorgensen MM, Baek R, Varming K Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles. 2015;4:26048. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25862471
- Gool EL, Stojanovic I, Schasfoort RBM, et al. Surface plasmon resonance is an analytically sensitive method for antigen profiling of extracellular vesicles. Clin Chem. 2017;63(10):1633–1641. Available from: http://www.clinchem.org/lookup/doi/10.1373/clinchem.2016.271049
- Zhu L, Wang K, Cui J, et al. Label-free quantitative detection of tumor-derived exosomes through surface plasmon resonance imaging. Anal Chem. 2014;86(17):8857–8864. Available from: http://pubs.acs.org/doi/10.1021/ac5023056
- Shao H, Im H, Castro CM, et al. New technologies for analysis of extracellular vesicles. Chem Rev. 2018;118(4):1917–1950. Available from: http://pubs.acs.org/doi/10.1021/acs.chemrev.7b00534
- Skotland T, Sandvig K, Llorente A Lipids in exosomes: current knowledge and the way forward. Prog Lipid Res. 2017;66:30–41. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0163782716300492
- Record M, Carayon K, Poirot M, et al. Exosomes as new vesicular lipid transporters involved in cell-cell communication and various pathophysiologies. Biochim Biophys Acta. 2014;1841(1):108–120. Available from: http://linkinghub.elsevier.com/retrieve/pii/S1388198113002199
- Nielsen MH, Beck-Nielsen H, Andersen MN, et al. A flow cytometric method for characterization of circulating cell-derived microparticles in plasma. J Extracell Vesicles. 2014;3(1):20795. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v3.20795
- de Gassart A, Geminard C, Fevrier B, et al. Lipid raft-associated protein sorting in exosomes. Blood. 2003;102(13):4336–4344. Available from: http://www.ncbi.nlm.nih.gov/pubmed/12881314
- Gualerzi A, Niada S, Giannasi C, et al. Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells. Sci Rep. 2017;7(1):9820. Available from: http://www.nature.com/articles/s41598-017-10448-1
- Neri T, Lombardi S, Faìta F, et al. Pirfenidone inhibits p38-mediated generation of procoagulant microparticles by human alveolar epithelial cells. Pulm Pharmacol Ther. 2016;39:1–6. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27237042
- de Rond L, van der Pol E, Hau CM, et al. Comparison of generic fluorescent markers for detection of extracellular vesicles by flow cytometry. Clin Chem. 2018;64(4):680–689. Available from: http://www.clinchem.org/lookup/doi/10.1373/clinchem.2017.278978
- Ullal AJ, Pisetsky DS, Reich CF. Use of SYTO 13, a fluorescent dye binding nucleic acids, for the detection of microparticles in in vitro systems. Cytometry A. 2010;77(3):294–301.
- Sansone P, Savini C, Kurelac I, et al. Packaging and transfer of mitochondrial DNA via exosomes regulate escape from dormancy in hormonal therapy-resistant breast cancer. Proc Natl Acad Sci U S A. 2017;114(43):E9066–75. Available from: http://www.pnas.org/lookup/doi/10.1073/pnas.1704862114
- Crescitelli R, Lässer C, Szabó TG, et al. Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes. J Extracell Vesicles. 2013;2(1):20677. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24223256
- Nolte-’t Hoen EN, Buermans HP, Waasdorp M, et al. Deep sequencing of RNA from immune cell-derived vesicles uncovers the selective incorporation of small non-coding RNA biotypes with potential regulatory functions. Nucleic Acids Res. 2012. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22821563
- Villarroya-Beltri C, Gutierrez-Vazquez C, Sanchez-Cabo F, et al. Sumoylated hnRNPA2B1 controls the sorting of miRNAs into exosomes through binding to specific motifs. Nat Commun. 2013;4:2980. [ 2013/12/21]. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24356509
- Vojtech L, Woo S, Hughes S, et al. Exosomes in human semen carry a distinctive repertoire of small non-coding RNAs with potential regulatory functions. Nucleic Acids Res. 2014;42(11):7290–7304. Available from: https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gku347
- Tosar JP, Gambaro F, Sanguinetti J, et al. Assessment of small RNA sorting into different extracellular fractions revealed by high-throughput sequencing of breast cell lines. Nucleic Acids Res. 2015;43(11):5601–5616. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25940616
- van Balkom BWM, Eisele AS, Pegtel DM, et al. Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting. J Extracell Vesicles. 2015;4(1):26760. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v4.26760
- Li K, Rodosthenous RS, Kashanchi F, et al. Advances, challenges, and opportunities in extracellular RNA biology: insights from the NIH exRNA strategic workshop. JCI Insight. 2018;3(7). Available from: https://insight.jci.org/articles/view/98942
- Chen M, Xu R, Ji H, et al. Transcriptome and long noncoding RNA sequencing of three extracellular vesicle subtypes released from the human colon cancer LIM1863 cell line. Sci Rep. 2016;6(1):38397. Available from: http://www.nature.com/articles/srep38397
- Lai CP, Kim EY, Badr CE., et al. Visualization and tracking of tumour extracellular vesicle delivery and RNA translation using multiplexed reporters. Nat Commun. 2015;6(May):7029.
- Ter-Ovanesyan D, Kowal EJK, Regev A, et al. Imaging of isolated extracellular vesicles using fluorescence microscopy. Methods Mol Biol. 2017;1660:233–241. Available from: http://link.springer.com/10.1007/978-1-4939-7253-1_19
- Wu Y, Deng W, Klinke DJ. Exosomes: improved methods to characterize their morphology, RNA content, and surface protein biomarkers. Analyst. 2015;140(19):6631–6642. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26332016
- Linares R, Tan S, Gounou C, et al. High-speed centrifugation induces aggregation of extracellular vesicles. J Extracell Vesicles. 2015;4(0):29509. Available from: http://www.journalofextracellularvesicles.net/index.php/jev/article/view/29509
- Höög JL, Lötvall J Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. J Extracell Vesicles. 2015;4:28680. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26563734
- Sharma S, Rasool HI, Palanisamy V, et al. Structural-mechanical characterization of nanoparticle exosomes in human saliva, using correlative AFM, FESEM, and force spectroscopy. ACS Nano. 2010;4(4):1921–1926. Available from: http://www.ncbi.nlm.nih.gov/pubmed/20218655
- Treps L, Perret R, Edmond S, et al. Glioblastoma stem-like cells secrete the pro-angiogenic VEGF-A factor in extracellular vesicles. J Extracell Vesicles. 2017;6(1):1359479. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1359479
- Chen C, Zong S, Wang Z, et al. Imaging and intracellular tracking of cancer-derived exosomes using single-molecule localization-based super-resolution microscope. ACS Appl Mater Interfaces. 2016;8(39):25825–25833. Available from: http://pubs.acs.org/doi/10.1021/acsami.6b09442
- Mehdiani A, Maier A, Pinto A, et al. An innovative method for exosome quantification and size measurement. J Vis Exp. 2015;95:50974. Available from: http://www.jove.com/video/50974/an-innovative-method-for-exosome-quantification-and-size-measurement
- Tatischeff I, Larquet E, Falcón-Pérez JM, et al. Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy. J Extracell Vesicles. 2012;1(1):19179. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v1i0.19179
- Carney RP, Hazari S, Colquhoun M, et al. Multispectral optical tweezers for biochemical fingerprinting of CD9-positive exosome subpopulations. Anal Chem. 2017;89(10):5357–5363. Available from: http://pubs.acs.org/doi/10.1021/acs.analchem.7b00017
- Smith ZJ, Lee C, Rojalin T, et al. Single exosome study reveals subpopulations distributed among cell lines with variability related to membrane content. J Extracell Vesicles. 2015;4(1):28533. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v4.28533
- Stoner SA, Duggan E, Condello D, et al. High sensitivity flow cytometry of membrane vesicles. Cytom Part A. 2016;89(2):196–206. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26484737
- Nolan JP, Jones JC. Detection of platelet vesicles by flow cytometry. Platelets. 2017;28(3):256–262. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28277059
- Sitar S, Kejžar A, Pahovnik D, et al. Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation. Anal Chem. 2015;87(18):9225–9233. Available from: http://pubs.acs.org/doi/10.1021/acs.analchem.5b01636
- Heusermann W, Hean J, Trojer D, et al. Exosomes surf on filopodia to enter cells at endocytic hot spots, traffic within endosomes, and are targeted to the ER. J Cell Biol. 2016;213(2):173–184. Available from: http://www.ncbi.nlm.nih.gov/pubmed/27114500
- Wyss R, Grasso L, Wolf C, et al. Molecular and dimensional profiling of highly purified extracellular vesicles by fluorescence fluctuation spectroscopy. Anal Chem. 2014;86(15):7229–7233. Available from: http://pubs.acs.org/doi/10.1021/ac501801m
- Baietti MF, Zhang Z, Mortier E, et al. Syndecan–syntenin–ALIX regulates the biogenesis of exosomes. Nat Cell Biol. 2012;14(7):677–685.
- Erdbrügger U, Rudy CK, Etter ME, et al. Imaging flow cytometry elucidates limitations of microparticle analysis by conventional flow cytometry. Cytometry A. 2014;85(9):756–770.
- Headland SE, Jones HR, Asv D, et al. Cutting-edge analysis of extracellular microparticles using ImageStream(X) imaging flow cytometry. Sci Rep. 2014;4(1):5237. Available from: http://www.nature.com/articles/srep05237
- Lee K, Fraser K, Ghaddar B, et al. Multiplexed profiling of single extracellular vesicles. ACS Nano. 2018;12(1):494–503. Available from: http://pubs.acs.org/doi/10.1021/acsnano.7b07060
- Daaboul GG, Lopez CA, Yurt A, et al. Label-free optical biosensors for virus detection and characterization. IEEE J Sel Top Quantum Electron. 2012;18(4):1422–1433.
- Daaboul GG, Freedman DS, Scherr SM, et al. Enhanced light microscopy visualization of virus particles from Zika virus to filamentous ebolaviruses. PLoS One. 2017;12(6):e0179728.
- van der Pol E, Sturk A, van Leeuwen T, et al., ISTH-SSC-VB Working group. Standardization of extracellular vesicle measurements by flow cytometry through vesicle diameter approximation. J Thromb Haemost. 2018;16(6):1236–1245.
- Cvjetkovic A, Jang SC, Konečná B, et al. Detailed analysis of protein topology of extracellular vesicles-evidence of unconventional membrane protein orientation. Sci Rep. 2016;6(1):36338. Available from: http://www.nature.com/articles/srep36338
- Deregibus MC, Cantaluppi V, Calogero R, et al. Endothelial progenitor cell derived microvesicles activate an angiogenic program in endothelial cells by a horizontal transfer of mRNA. Blood. 2007;110(7):2440–2448. Available from: http://www.bloodjournal.org/cgi/doi/10.1182/blood-2007-03-078709
- Sharma A, Mariappan M, Appathurai S, et al. In vitro dissection of protein translocation into the mammalian endoplasmic reticulum. Methods Mol Biol. 2010;619:339–363. Available from: http://link.springer.com/10.1007/978-1-60327-412-8_20
- Sung BH, Weaver AM. Exosome secretion promotes chemotaxis of cancer cells. Cell Adh Migr. 2017;11(2):187–195. Available from: https://www.tandfonline.com/doi/full/10.1080/19336918.2016.1273307
- Osteikoetxea X, Sódar B, Németh A, et al. Differential detergent sensitivity of extracellular vesicle subpopulations. Org Biomol Chem. 2015;13(38):9775–9782. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26264754
- Parolini I, Federici C, Raggi C, et al. Microenvironmental pH is a key factor for exosome traffic in tumor cells. J Biol Chem. 2009;284(49):34211–34222. Available from: http://www.jbc.org/lookup/doi/10.1074/jbc.M109.041152
- Franzen CA, Simms PE, Van Huis AF, et al. Characterization of uptake and internalization of exosomes by bladder cancer cells. Biomed Res Int. 2014;2014:619829. Available from: http://www.hindawi.com/journals/bmri/2014/619829/
- Christianson HC, Svensson KJ, van Kuppevelt TH, et al. Cancer cell exosomes depend on cell-surface heparan sulfate proteoglycans for their internalization and functional activity. Proc Natl Acad Sci U S A. 2013;110(43):17380–17385. Available from: http://www.pnas.org/cgi/doi/10.1073/pnas.1304266110
- Mulcahy LA, Pink RC, Carter DRF. Routes and mechanisms of extracellular vesicle uptake. J Extracell Vesicles. 2014;3:24641. Available from: https://www.tandfonline.com/doi/full/10.3402/jev.v3.24641
- Wahlgren J, Karlson TDL, Glader P, et al. Activated human T cells secrete exosomes that participate in IL-2 mediated immune response signaling. PLoS One. 2012;7(11):e49723. Available from: http://dx.plos.org/10.1371/journal.pone.0049723
- Szabó GT, Tarr B, Pálóczi K, et al. Critical role of extracellular vesicles in modulating the cellular effects of cytokines. Cell Mol Life Sci. 2014;71(20):4055–4067. Available from: http://link.springer.com/10.1007/s00018-014-1618-z
- Gámez-Valero A, Monguió-Tortajada M, Carreras-Planella L, et al. Size-exclusion chromatography-based isolation minimally alters extracellular vesicles’ characteristics compared to precipitating agents. Sci Rep. 2016;6(1):33641. Available from: http://www.nature.com/articles/srep33641
- Paolini L, Zendrini A, Di Noto G, et al. Residual matrix from different separation techniques impacts exosome biological activity. Sci Rep. 2016;6(1):23550. Available from: http://www.nature.com/articles/srep23550
- Gyorgy B, Modos K, Pallinger E, et al. Detection and isolation of cell-derived microparticles are compromised by protein complexes resulting from shared biophysical parameters. Blood. 2011;117(4):e39–48. Available from: http://www.ncbi.nlm.nih.gov/pubmed/21041717
- Benedikter BJ, Bouwman FG, Vajen T, et al. Ultrafiltration combined with size exclusion chromatography efficiently isolates extracellular vesicles from cell culture media for compositional and functional studies. Sci Rep. 2017;7(1):15297. Available from: http://www.nature.com/articles/s41598-017-15717-7
- Trajkovic K, Hsu C, Chiantia S, et al. Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science. 2008;319(5867):1244–1247. Available from: http://www.sciencemag.org/cgi/doi/10.1126/science.1153124
- Figuera-Losada M, Stathis M, Dorskind JM, et al. Cambinol, a novel inhibitor of neutral sphingomyelinase 2 shows neuroprotective properties. PLoS One. 2015;10(5):e0124481. Available from: http://dx.plos.org/10.1371/journal.pone.0124481
- Dinkins MB, Enasko J, Hernandez C, et al. Neutral sphingomyelinase-2 deficiency ameliorates alzheimer’s disease pathology and improves cognition in the 5XFAD mouse. J Neurosci. 2016;36(33):8653–8667. Available from: http://www.jneurosci.org/lookup/doi/10.1523/JNEUROSCI.1429-16.2016
- Cruz FF, Borg ZD, Goodwin M, et al. Systemic administration of human bone marrow-derived mesenchymal stromal cell extracellular vesicles ameliorates aspergillus hyphal extract-induced allergic airway inflammation in immunocompetent mice. Stem Cells Transl Med. 2015;4(11):1302–1316.
- Villarroya-Beltri C, Baixauli F, Mittelbrunn M, et al. ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins. Nat Commun. 2016;7:13588. Available from: http://www.nature.com/doifinder/10.1038/ncomms13588
- Savina A, Vidal M, Colombo MI. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002;115(Pt 12):2505–2515. Available from: http://www.ncbi.nlm.nih.gov/pubmed/12045221
- Ostrowski M, Carmo NB, Krumeich S, et al. Rab27a and Rab27b control different steps of the exosome secretion pathway. Nat Cell Biol. 2010;12(1):13–19. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19966785
- Hsu C, Morohashi Y, Yoshimura S-I, et al. Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-C. J Cell Biol. 2010;189(2):223–232. Available from: http://www.ncbi.nlm.nih.gov/pubmed/20404108
- Hyenne V, Apaydin A, Rodriguez D, et al. RAL-1 controls multivesicular body biogenesis and exosome secretion. J Cell Biol. 2015;211(1):27–37. Available from: http://www.jcb.org/lookup/doi/10.1083/jcb.201504136
- Gross JC, Chaudhary V, Bartscherer K, et al. Active Wnt proteins are secreted on exosomes. Nat Cell Biol. 2012;14(10):1036–1045. Available from: http://www.nature.com/articles/ncb2574
- Imjeti NS, Menck K, Egea-Jimenez AL, et al. Syntenin mediates SRC function in exosomal cell-to-cell communication. Proc Natl Acad Sci U S A. 2017;114(47):12495–12500. Available from: http://www.pnas.org/lookup/doi/10.1073/pnas.1713433114
- Sinha S, Hoshino D, Hong NH, et al. Cortactin promotes exosome secretion by controlling branched actin dynamics. J Cell Biol. 2016;214(2):197–213. Available from: http://www.jcb.org/lookup/doi/10.1083/jcb.201601025
- Jackson CE, Scruggs BS, Schaffer JE, et al. Effects of inhibiting VPS4 support a general role for ESCRTs in extracellular vesicle biogenesis. Biophys J. 2017;113(6):1342–1352. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0006349517305714
- Chalmin F, Ladoire S, Mignot G, et al. Membrane-associated Hsp72 from tumor-derived exosomes mediates STAT3-dependent immunosuppressive function of mouse and human myeloid-derived suppressor cells. J Clin Invest. 2010;120(2):457–471. Available from: http://www.jci.org/articles/view/40483
- Montecalvo A, Larregina AT, Shufesky WJ, et al. Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes. Blood. 2012;119(3):756–766. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22031862
- Savina A, Furlán M, Vidal M, et al. Exosome release is regulated by a calcium-dependent mechanism in K562 cells. J Biol Chem. 2003;278(22):20083–20090. Available from: http://www.jbc.org/lookup/doi/10.1074/jbc.M301642200
- Minakaki G, Menges S, Kittel A, et al. Autophagy inhibition promotes SNCA/alpha-synuclein release and transfer via extracellular vesicles with a hybrid autophagosome-exosome-like phenotype. Autophagy. 2018;14(1):98–119. Available from: https://www.tandfonline.com/doi/full/10.1080/15548627.2017.1395992
- Edgar JR, Manna PT, Nishimura S, et al. Tetherin is an exosomal tether. Elife. 2016;5:17180. Available from: https://elifesciences.org/articles/17180
- Atai NA, Balaj L, van Veen H, et al. Heparin blocks transfer of extracellular vesicles between donor and recipient cells. J Neurooncol. 2013. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24002181
- Wang Q, Lu Q Plasma membrane-derived extracellular microvesicles mediate non-canonical intercellular NOTCH signaling. Nat Commun. 2017;8(1):709. Available from: http://www.nature.com/articles/s41467-017-00767-2
- Nabhan JF, Hu R, Oh RS, et al. Formation and release of arrestin domain-containing protein 1-mediated microvesicles (ARMMs) at plasma membrane by recruitment of TSG101 protein. Proc Natl Acad Sci U S A. 2012;109(11):4146–4151. Available from: http://www.pnas.org/cgi/doi/10.1073/pnas.1200448109
- Muralidharan-Chari V, Clancy J, Plou C, et al. ARF6-regulated shedding of tumor cell-derived plasma membrane microvesicles. Curr Biol. 2009;19(22):1875–1885. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0960982209017722
- Burger D, Montezano AC, Nishigaki N, et al. Endothelial microparticle formation by angiotensin II is mediated via Ang II receptor type I/NADPH oxidase/Rho kinase pathways targeted to lipid rafts. Arterioscler Thromb Vasc Biol. 2011;31(8):1898–1907. Available from: http://atvb.ahajournals.org/cgi/doi/10.1161/ATVBAHA.110.222703
- Gao C, Li R, Liu Y, et al. Rho-kinase-dependent F-actin rearrangement is involved in the release of endothelial microparticles during IFN-α-induced endothelial cell apoptosis. J Trauma Acute Care Surg. 2012;73(5):1152–1160. Available from: http://content.wkhealth.com/linkback/openurl?sid=WKPTLP:landingpage&an=01586154-201211000-00017
- Yu X, Xu J, Liu W, et al. Bubbles induce endothelial microparticle formation via a calcium-dependent pathway involving flippase inactivation and rho kinase activation. Cell Physiol Biochem. 2018;46(3):965–974. Available from: https://www.karger.com/Article/FullText/488825
- Di Vizio D, Kim J, Hager MH, et al. Oncosome formation in prostate cancer: association with a region of frequent chromosomal deletion in metastatic disease. Cancer Res. 2009;69(13):5601–5609. Available from: http://cancerres.aacrjournals.org/cgi/doi/10.1158/0008-5472.CAN-08-3860
- Schwechheimer C, Kuehn MJ. Outer-membrane vesicles from Gram-negative bacteria: biogenesis and functions. Nat Rev Microbiol 2015;13(10):605–619. Available from: http://www.nature.com/articles/nrmicro3525
- Colombo M, Raposo G, Théry C Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. Annu Rev Cell Dev Biol. 2014;30(1):255–289. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25288114
- Romancino DP, Paterniti G, Campos Y, et al. Identification and characterization of the nano-sized vesicles released by muscle cells. FEBS Lett. 2013;587(9):1379–1384.
- Booth AM, Fang Y, Fallon JK, et al. Exosomes and HIV Gag bud from endosome-like domains of the T cell plasma membrane. J Cell Biol. 2006;172(6):923–935. Available from: http://www.ncbi.nlm.nih.gov/pubmed/16533950
- Hoang TQ, Rampon C, Freyssinet J-M, et al. A method to assess the migration properties of cell-derived microparticles within a living tissue. Biochim Biophys Acta. 2011;1810(9):863–866. Available from: http://linkinghub.elsevier.com/retrieve/pii/S0304416511001061
- Menck K, Sönmezer C, Worst TS, et al. Neutral sphingomyelinases control extracellular vesicles budding from the plasma membrane. J Extracell Vesicles. 2017;6(1):1378056. Available from: https://www.tandfonline.com/doi/full/10.1080/20013078.2017.1378056
- Bobrie A, Colombo M, Krumeich S, et al. Diverse subpopulations of vesicles secreted by different intracellular mechanisms are present in exosome preparations obtained by differential ultracentrifugation. J Extracell Vesicles. 2012;1:18297.
- Peinado H, Alečković M, Lavotshkin S, et al. Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nat Med. 2012;18(6):883–891. Available from: http://www.ncbi.nlm.nih.gov/pubmed/22635005
- Kim DK, Kang B, Kim OY, et al. EVpedia: an integrated database of high-throughput data for systemic analyses of extracellular vesicles. J Extracell Vesicles. 2013;2. Available from: http://www.ncbi.nlm.nih.gov/pubmed/24009897
- Kim D-K, Lee J, Kim SR, et al. EVpedia: a community web portal for extracellular vesicles research. Bioinformatics. 2015;31(6):933–939. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25388151
- Kalra H, Simpson RJ, Ji H, et al. Vesiclepedia: a compendium for extracellular vesicles with continuous community annotation. PLoS Biol. 2012;10(12):e1001450.
- Mathivanan S, Simpson RJ. ExoCarta: A compendium of exosomal proteins and RNA. Proteomics. 2009;9(21):4997–5000. Available from: http://www.ncbi.nlm.nih.gov/pubmed/19810033
- Subramanian SL, Kitchen RR, Alexander R, et al. Integration of extracellular RNA profiling data using metadata, biomedical ontologies and linked data technologies. J Extracell Vesicles. 2015;4:27497. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26320941