Blue light treatment of Pseudomonas aeruginosa: Strong bactericidal activity, synergism with antibiotics and inactivation of virulence factors

Reference

• Balasubramanian D, Schneper L, Kumari H, Mathee K. A dynamic and intricate regulatory network determines Pseudomonas aeruginosa virulence. Nucleic Acids Res 2013; 41:1-20; PMID:23143271; http://dx.doi.org/10.1093/nar/gks1039
   PubMed Web of Science ®Google Scholar
• Rybtke M, Hultqvist LD, Givskov M, Tolker-Nielsen T. Pseudomonas aeruginosa Biofilm Infections: Community Structure, Antimicrobial Tolerance and Immune Response. J Mol Biol 2015; 427:3628-45; PMID:26319792; http://dx.doi.org/10.1016/j.jmb.2015.08.016
   PubMed Web of Science ®Google Scholar
• Serra R, Grande R, Butrico L, Rossi A, Settimio UF, Caroleo B, Amato B, Gallelli L, de FS. Chronic wound infections: the role of Pseudomonas aeruginosa and Staphylococcus aureus. Expert Rev Anti Infect Ther 2015; 13:605-13; PMID:25746414; http://dx.doi.org/10.1586/14787210.2015.1023291
   PubMed Web of Science ®Google Scholar
• Mayaud C, Parrot A, Cadranel J. Pyogenic bacterial lower respiratory tract infection in human immunodeficiency virus-infected patients. Eur Respir J Suppl 2002; 36:28s-39s; PMID:12168745; http://dx.doi.org/10.1183/09031936.02.00400602
   PubMedGoogle Scholar
• Vento S, Cainelli F, Temesgen Z. Lung infections after cancer chemotherapy. Lancet Oncol 2008; 9:982-92; PMID:19071255; http://dx.doi.org/10.1016/S1470-2045(08)70255-9
   PubMed Web of Science ®Google Scholar
• Potron A, Poirel L, Nordmann P. Emerging broad-spectrum resistance in Pseudomonas aeruginosa and Acinetobacter baumannii: Mechanisms and epidemiology. Int J Antimicrob Agents 2015; 45:568-85; PMID:25857949; http://dx.doi.org/10.1016/j.ijantimicag.2015.03.001
   PubMed Web of Science ®Google Scholar
• Clatworthy AE, Pierson E, Hung DT. Targeting virulence: a new paradigm for antimicrobial therapy. Nat Chem Biol 2007; 3:541-8; PMID:17710100; http://dx.doi.org/10.1038/nchembio.2007.24
   PubMed Web of Science ®Google Scholar
• Merrell DS, Falkow S. Frontal and stealth attack strategies in microbial pathogenesis. Nature 2004; 430:250-6; PMID:15241423; http://dx.doi.org/10.1038/nature02760
   PubMed Web of Science ®Google Scholar
• Sommer R, Joachim I, Wagner S, Titz A. New approaches to control infections: anti-biofilm strategies against gram-negative bacteria. Chimia (Aarau) 2013; 67:286-90; PMID:23967708; http://dx.doi.org/10.2533/chimia.2013.286
   PubMed Web of Science ®Google Scholar
• Rasmussen TB, Givskov M. Quorum-sensing inhibitors as anti-pathogenic drugs. Int J Med Microbiol 2006; 296:149-61; PMID:16503194; http://dx.doi.org/10.1016/j.ijmm.2006.02.005
   PubMed Web of Science ®Google Scholar
• Wagner S, Sommer R, Hinsberger S, Lu C, Hartmann RW, Empting M, Titz A. Novel Strategies for the Treatment of Pseudomonas aeruginosa Infections. J Med Chem 2016; 59:5929-69; PMID:26804741; http://dx.doi.org/10.1021/acs.jmedchem.5b01698
   PubMed Web of Science ®Google Scholar
• Kuang Z, Hao Y, Walling BE, Jeffries JL, Ohman DE, Lau GW. Pseudomonas aeruginosa elastase provides an escape from phagocytosis by degrading the pulmonary surfactant protein-A. PLoS One 2011; 6:e27091; PMID:22069491; http://dx.doi.org/10.1371/journal.pone.0027091
   PubMed Web of Science ®Google Scholar
• Caballero AR, Moreau JM, Engel LS, Marquart ME, Hill JM, O'Callaghan RJ. Pseudomonas aeruginosa protease IV enzyme assays and comparison to other Pseudomonas proteases. Anal Biochem 2001; 290:330-7; PMID:11237336; http://dx.doi.org/10.1006/abio.2001.4999
   PubMed Web of Science ®Google Scholar
• Berka RM, Vasil ML. Phospholipase C (heat-labile hemolysin) of Pseudomonas aeruginosa: purification and preliminary characterization. J Bacteriol 1982; 152:239-45; PMID:6811552
   PubMed Web of Science ®Google Scholar
• Rossignol G, Merieau A, Guerillon J, Veron W, Lesouhaitier O, Feuilloley MG, Orange N. Involvement of a phospholipase C in the hemolytic activity of a clinical strain of Pseudomonas fluorescens. BMC Microbiol 2008; 8:189; PMID:18973676; http://dx.doi.org/10.1186/1471-2180-8-189
   PubMed Web of Science ®Google Scholar
• Bush K, Courvalin P, Dantas G, Davies J, Eisenstein B, Huovinen P, Jacoby GA, Kishony R, Kreiswirth BN, Kutter E, et al. Tackling antibiotic resistance. Nat Rev Microbiol 2011; 9:894-6; PMID:22048738; http://dx.doi.org/10.1038/nrmicro2693
   PubMed Web of Science ®Google Scholar
• Dai T, Tegos GP, Zhiyentayev T, Mylonakis E, Hamblin MR. Photodynamic therapy for methicillin-resistant Staphylococcus aureus infection in a mouse skin abrasion model. Lasers Surg Med 2010; 42:38-44; PMID:20077489; http://dx.doi.org/10.1002/lsm.20887
   PubMed Web of Science ®Google Scholar
• Dai T, Tegos GP, Lu Z, Huang L, Zhiyentayev T, Franklin MJ, Baer DG, Hamblin MR. Photodynamic therapy for Acinetobacter baumannii burn infections in mice. Antimicrob Agents Chemother 2009; 53:3929-34; PMID:19564369; http://dx.doi.org/10.1128/AAC.00027-09
   PubMed Web of Science ®Google Scholar
• Dai T, Garcia B, Murray CK, Vrahas MS, Hamblin MR. UVC light prophylaxis for cutaneous wound infections in mice. Antimicrob Agents Chemother 2012; 56:3841-8; PMID:22564833; http://dx.doi.org/10.1128/AAC.00161-12
   PubMed Web of Science ®Google Scholar
• Dai T, Kharkwal GB, Zhao J, St Denis TG, Wu Q, Xia Y, Huang L, Sharma SK, d'Enfert C, Hamblin MR. Ultraviolet-C light for treatment of Candida albicans burn infection in mice. Photochem Photobiol 2011; 87:342-9; PMID:21208209; http://dx.doi.org/10.1111/j.1751-1097.2011.00886.x
   PubMed Web of Science ®Google Scholar
• Waldman G. Introduction to light : the physics of light, vision, and color. 193. Mineola: Dover Publications, 2002
   Google Scholar
• Grinholc M, Rapacka-Zdonczyk A, Rybak B, Szabados F, Bielawski KP. Multiresistant Strains Are as Susceptible to Photodynamic Inactivation as Their Naive Counterparts: Protoporphyrin IX-Mediated Photoinactivation Reveals Differences Between Methicillin-Resistant and Methicillin-Sensitive Staphylococcus aureus Strains. Photomed Laser Surg 2014; 32:121-9; PMID:24527879; http://dx.doi.org/10.1089/pho.2013.3663
   PubMed Web of Science ®Google Scholar
• Dai T, Vrahas MS, Murray CK, Hamblin MR. Ultraviolet C irradiation: an alternative antimicrobial approach to localized infections? Expert Rev Anti Infect Ther 2012; 10:185-95; PMID:22339192; http://dx.doi.org/10.1586/eri.11.166
   PubMed Web of Science ®Google Scholar
• Dai T, Gupta A, Huang YY, Yin R, Murray CK, Vrahas MS, Sherwood ME, Tegos GP, Hamblin MR. Blue light rescues mice from potentially fatal Pseudomonas aeruginosa burn infection: efficacy, safety, and mechanism of action. Antimicrob Agents Chemother 2013; 57:1238-45; PMID:23262998; http://dx.doi.org/10.1128/AAC.01652-12
   PubMed Web of Science ®Google Scholar
• Enwemeka CS, Williams D, Hollosi S, Yens D, Enwemeka SK. Visible 405 nm SLD light photo-destroys methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Lasers Surg Med 2008; 40:734-7; PMID:19065556; http://dx.doi.org/10.1002/lsm.20724
   PubMed Web of Science ®Google Scholar
• Wheeland RG, Dhawan S. Evaluation of self-treatment of mild-to-moderate facial acne with a blue light treatment system. J Drugs Dermatol 2011; 10:596-602; PMID:21637900
   PubMed Web of Science ®Google Scholar
• Halstead FD, Thwaite JE, Burt R, Laws TR, Raguse M, Moeller R, Webber MA, Oppenheim BA. The antibacterial activity of blue light against nosocomial wound pathogens growing planktonically and as mature biofilms. Appl Environ Microbiol 2016; PMID:27129967
   PubMed Web of Science ®Google Scholar
• Dai T, Gupta A, Murray CK, Vrahas MS, Tegos GP, Hamblin MR. Blue light for infectious diseases: Propionibacterium acnes, Helicobacter pylori, and beyond? Drug Resist Updat 2012; 15:223-36; PMID:22846406; http://dx.doi.org/10.1016/j.drup.2012.07.001
   PubMed Web of Science ®Google Scholar
• Grinholc M, Rodziewicz A, Forys K, Rapacka-Zdonczyk A, Kawiak A, Domachowska A, Golunski G, Wolz C, Mesak L, Becker K, et al. Fine-tuning recA expression in Staphylococcus aureus for antimicrobial photoinactivation: importance of photo-induced DNA damage in the photoinactivation mechanism. Appl Microbiol Biotechnol 2015; 99:9161-76; PMID:26252968; http://dx.doi.org/10.1007/s00253-015-6863-z
   PubMed Web of Science ®Google Scholar
• Dadras S, Mohajerani E, Eftekhar F, Hosseini M. Different photoresponses of Staphylococcus aureus and Pseudomonas aeruginosa to 514, 532, and 633 nm low level lasers in vitro. Curr Microbiol 2006; 53:282-6; PMID:16941244; http://dx.doi.org/10.1007/s00284-005-0490-3
   PubMed Web of Science ®Google Scholar
• Kim S, Kim J, Lim W, Jeon S, Kim O, Koh JT, Kim CS, Choi H, Kim O. In vitro bactericidal effects of 625, 525, and 425 nm wavelength (red, green, and blue) light-emitting diode irradiation. Photomed Laser Surg 2013; 31:554-62; PMID:24138193; http://dx.doi.org/10.1089/pho.2012.3343
   PubMed Web of Science ®Google Scholar
• Cieplik F, Spath A, Leibl C, Gollmer A, Regensburger J, Tabenski L, Hiller KA, Maisch T, Schmalz G. Blue light kills Aggregatibacter actinomycetemcomitans due to its endogenous photosensitizers. Clin Oral Investig 2014; 18:1763-9; PMID:24297656; http://dx.doi.org/10.1007/s00784-013-1151-8
   PubMed Web of Science ®Google Scholar
• Soukos NS, Som S, Abernethy AD, Ruggiero K, Dunham J, Lee C, Doukas AG, Goodson JM. Phototargeting oral black-pigmented bacteria. Antimicrob Agents Chemother 2005; 49:1391-6; PMID:15793117; http://dx.doi.org/10.1128/AAC.49.4.1391-1396.2005
   PubMed Web of Science ®Google Scholar
• Maclean M, McKenzie K, Anderson JG, Gettinby G, MacGregor SJ. 405 nm light technology for the inactivation of pathogens and its potential role for environmental disinfection and infection control. J Hosp Infect 2014; 88:1-11; PMID:25066049; http://dx.doi.org/10.1016/j.jhin.2014.06.004
   PubMed Web of Science ®Google Scholar
• Kleinpenning MM, Smits T, Frunt MH, van Erp PE, van de Kerkhof PC, Gerritsen RM. Clinical and histological effects of blue light on normal skin. Photodermatol Photoimmunol Photomed 2010; 26:16-21; PMID:20070834; http://dx.doi.org/10.1111/j.1600-0781.2009.00474.x
   PubMed Web of Science ®Google Scholar
• Amin RM, Bhayana B, Hamblin MR, Dai T. Antimicrobial blue light inactivation of Pseudomonas aeruginosa by photo-excitation of endogenous porphyrins: In vitro and in vivo studies. Lasers Surg Med 2016; PMID:26891084
   PubMed Web of Science ®Google Scholar
• Dodd CER, Sharman RL, Bloomfield SF, Booth IR, Stewart GSAB. Inimical processes: Bacterial self-destruction and sub-lethal injury. Trends Food Sci Technol 1997; 8:238-41; http://dx.doi.org/10.1016/S0924-2244(97)01043-1
   Web of Science ®Google Scholar
• ASM. Antimicrobial Agents and Chemotherapy, Instructions to Authors. http://aac.asm.org/site/misc/2016AprilAACITA.pdf. 2016
   Google Scholar
• Lau GW, Ran H, Kong F, Hassett DJ, Mavrodi D. Pseudomonas aeruginosa pyocyanin is critical for lung infection in mice. Infect Immun 2004; 72:4275-8; PMID:15213173; http://dx.doi.org/10.1128/IAI.72.7.4275-4278.2004
   PubMed Web of Science ®Google Scholar
• Reszka KJ, Bilski PJ, Britigan BE. Quenching of singlet oxygen by pyocyanin and related phenazines. Photochem Photobiol 2010; 86:742-6; PMID:20408986; http://dx.doi.org/10.1111/j.1751-1097.2010.00728.x
   PubMed Web of Science ®Google Scholar
• Hawdon NA, Aval PS, Barnes RJ, Gravelle SK, Rosengren J, Khan S, Ciofu O, Johansen HK, Hoiby N, Ulanova M. Cellular responses of A549 alveolar epithelial cells to serially collected Pseudomonas aeruginosa from cystic fibrosis patients at different stages of pulmonary infection. FEMS Immunol Med Microbiol 2010; 59:207-20; PMID:20528926; http://dx.doi.org/10.1111/j.1574-695X.2010.00693.x
   PubMed Web of Science ®Google Scholar
• Griese M, Kappler M, Eismann C, Ballmann M, Junge S, Rietschel E, van Koningsbruggen-Rietschel S, Staab D, Rolinck-Werninghaus C, Mellies U, et al. Inhalation treatment with glutathione in patients with cystic fibrosis. A randomized clinical trial. Am J Respir Crit Care Med 2013; 188:83-9; PMID:23631796; http://dx.doi.org/10.1164/rccm.201303-0427OC
   PubMed Web of Science ®Google Scholar
• Kipnis E, Sawa T, Wiener-Kronish J. Targeting mechanisms of Pseudomonas aeruginosa pathogenesis. Med Mal Infect 2006; 36:78-91; PMID:16427231; http://dx.doi.org/10.1016/j.medmal.2005.10.007
   PubMed Web of Science ®Google Scholar
• Lau GW, Hassett DJ, Ran H, Kong F. The role of pyocyanin in Pseudomonas aeruginosa infection. Trends Mol Med 2004; 10:599-606; PMID:15567330; http://dx.doi.org/10.1016/j.molmed.2004.10.002
   PubMed Web of Science ®Google Scholar
• Das T, Kutty SK, Kumar N, Manefield M. Pyocyanin facilitates extracellular DNA binding to Pseudomonas aeruginosa influencing cell surface properties and aggregation. PLoS One 2013; 8:e58299; PMID:23505483; http://dx.doi.org/10.1371/journal.pone.0058299
   PubMed Web of Science ®Google Scholar
• Das MC, Sandhu P, Gupta P, Rudrapaul P, De UC, Tribedi P, Akhter Y, Bhattacharjee S. Attenuation of Pseudomonas aeruginosa biofilm formation by Vitexin: A combinatorial study with azithromycin and gentamicin. Sci Rep 2016; 6:23347; PMID:27000525; http://dx.doi.org/10.1038/srep23347
   PubMed Web of Science ®Google Scholar
• Comolli JC, Waite LL, Mostov KE, Engel JN. Pili binding to asialo-GM1 on epithelial cells can mediate cytotoxicity or bacterial internalization by Pseudomonas aeruginosa. Infect Immun 1999; 67:3207-14; PMID:10377092
   PubMed Web of Science ®Google Scholar
• de BS, Roger P, Dupuit F, Bajolet-Laudinat O, Fuchey C, Plotkowski MC, Puchelle E. Asialo GM1 is a receptor for Pseudomonas aeruginosa adherence to regenerating respiratory epithelial cells. Infect Immun 1996; 64:1582-8; PMID:8613364
   PubMed Web of Science ®Google Scholar
• Rio-Alvarez I, Rodriguez-Herva JJ, Martinez PM, Gonzalez-Melendi P, Garcia-Casado G, Rodriguez-Palenzuela P, Lopez-Solanilla E. Light regulates motility, attachment and virulence in the plant pathogen Pseudomonas syringae pv tomato DC3000. Environ Microbiol 2014; 16:2072-85; PMID:24033935; http://dx.doi.org/10.1111/1462-2920.12240
   PubMed Web of Science ®Google Scholar
• Komerik N, Wilson M, Poole S. The effect of photodynamic action on two virulence factors of gram-negative bacteria. Photochem Photobiol 2000; 72:676-80; PMID:11107854; http://dx.doi.org/10.1562/0031-8655(2000)072%3c0676:TEOPAO%3e2.0.CO;2
   PubMed Web of Science ®Google Scholar
• Sharma M, Bansal H, Gupta PK. Virulence of Pseudomonas aeruginosa cells surviving photodynamic treatment with toluidine blue. Curr Microbiol 2005; 50:277-80; PMID:15886909; http://dx.doi.org/10.1007/s00284-005-4473-1
   PubMed Web of Science ®Google Scholar
• Tubby S, Wilson M, Nair SP. Inactivation of staphylococcal virulence factors using a light-activated antimicrobial agent. BMC Microbiol 2009; 9:211; PMID:19804627; http://dx.doi.org/10.1186/1471-2180-9-211
   PubMed Web of Science ®Google Scholar
• Bartolomeu M, Rocha S, Cunha A, Neves MG, Faustino MA, Almeida A. Effect of Photodynamic Therapy on the Virulence Factors of Staphylococcus aureus. Front Microbiol 2016; 7:267; PMID:27014198; http://dx.doi.org/10.3389/fmicb.2016.00267
   PubMed Web of Science ®Google Scholar
• Kato IT, Prates RA, Sabino CP, Fuchs BB, Tegos GP, Mylonakis E, Hamblin MR, Ribeiro MS. Antimicrobial photodynamic inactivation inhibits Candida albicans virulence factors and reduces in vivo pathogenicity. Antimicrob Agents Chemother 2013; 57:445-51; PMID:23129051; http://dx.doi.org/10.1128/AAC.01451-12
   PubMed Web of Science ®Google Scholar
• Kadurugamuwa JL, Beveridge TJ. Virulence factors are released from Pseudomonas aeruginosa in association with membrane vesicles during normal growth and exposure to gentamicin: a novel mechanism of enzyme secretion. J Bacteriol 1995; 177:3998-4008; PMID:7608073
   PubMed Web of Science ®Google Scholar
• Kadurugamuwa JL, Beveridge TJ. Natural release of virulence factors in membrane vesicles by Pseudomonas aeruginosa and the effect of aminoglycoside antibiotics on their release. J Antimicrob Chemother 1997; 40:615-21; PMID:9421308; http://dx.doi.org/10.1093/jac/40.5.615
   PubMed Web of Science ®Google Scholar
• Crosby HA, Bion JF, Penn CW, Elliott TS. Antibiotic-induced release of endotoxin from bacteria in vitro. J Med Microbiol 1994; 40:23-30; PMID:8289210; http://dx.doi.org/10.1099/00222615-40-1-23
   PubMed Web of Science ®Google Scholar
• Prins JM, van Deventer SJ, Kuijper EJ, Speelman P. Clinical relevance of antibiotic-induced endotoxin release. Antimicrob Agents Chemother 1994; 38:1211-8; PMID:8092816; http://dx.doi.org/10.1128/AAC.38.6.1211
   PubMed Web of Science ®Google Scholar
• Cassidy CM, Donnelly RF, Elborn JS, Magee ND, Tunney MM. Photodynamic Antimicrobial Chemotherapy (PACT) in combination with antibiotics for treatment of Burkholderia cepacia complex infection. J Photochem Photobiol B 2012; 106:95-100; PMID:22079165; http://dx.doi.org/10.1016/j.jphotobiol.2011.10.010
   PubMed Web of Science ®Google Scholar
• Dastgheyb SS, Eckmann DM, Composto RJ, Hickok NJ. Photo-activated porphyrin in combination with antibiotics: therapies against Staphylococci. J Photochem Photobiol B 2013; 129:27-35; PMID:24148969; http://dx.doi.org/10.1016/j.jphotobiol.2013.09.006
   PubMed Web of Science ®Google Scholar
• Ronqui MR, de Aguiar Coletti TM, de Freitas LM, Miranda ET, Fontana CR. Synergistic antimicrobial effect of photodynamic therapy and ciprofloxacin. J Photochem Photobiol B 2016; 158:122-9; PMID:26971277; http://dx.doi.org/10.1016/j.jphotobiol.2016.02.036
   PubMed Web of Science ®Google Scholar
• Chibebe JJ, Fuchs BB, Sabino CP, Junqueira JC, Jorge AO, Ribeiro MS, Gilmore MS, Rice LB, Tegos GP, Hamblin MR, et al. Photodynamic and antibiotic therapy impair the pathogenesis of Enterococcus faecium in a whole animal insect model. PLoS One 2013; 8:e55926; PMID:23457486; http://dx.doi.org/10.1371/journal.pone.0055926
   PubMed Web of Science ®Google Scholar
• Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, Harbarth S, Hindler JF, Kahlmeter G, Olsson-Liljequist B, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 2012; 18:268-281; PMID:21793988; http://dx.doi.org/10.1111/j.1469-0691.2011.03570.x
   PubMed Web of Science ®Google Scholar
• CLSI. Performance standards for antimicrobial susceptibility testing. CLSI approved standard M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute, 2012
   Google Scholar
• Jett BD, Hatter KL, Huycke MM, Gilmore MS. Simplified agar plate method for quantifying viable bacteria. Biotechniques 1997; 23:648-50; PMID:9343684
   PubMed Web of Science ®Google Scholar
• Barry AL, Lasner RA. In-vitro methods for determining minimal lethal concentrations of antimicrobial agents. Am J Clin Pathol 1979; 71:88-92; PMID:105628; http://dx.doi.org/10.1093/ajcp/71.1.88
   PubMed Web of Science ®Google Scholar
• Kohanski MA, DePristo MA, Collins JJ. Sublethal antibiotic treatment leads to multidrug resistance via radical-induced mutagenesis. Mol Cell 2010; 37:311-20; PMID:20159551; http://dx.doi.org/10.1016/j.molcel.2010.01.003
   PubMed Web of Science ®Google Scholar
• Latimer J, Forbes S, McBain AJ. Attenuated virulence and biofilm formation in Staphylococcus aureus following sublethal exposure to triclosan. Antimicrob Agents Chemother 2012; 56:3092-100; PMID:22430975; http://dx.doi.org/10.1128/AAC.05904-11
   PubMed Web of Science ®Google Scholar
• Andersson DI, Hughes D. Microbiological effects of sublethal levels of antibiotics. Nat Rev Microbiol 2014; 12:465-78; PMID:24861036; http://dx.doi.org/10.1038/nrmicro3270
   PubMed Web of Science ®Google Scholar
• El-Mowafy SA, bd El Galil KH, El-Messery SM, Shaaban MI. Aspirin is an efficient inhibitor of quorum sensing, virulence and toxins in Pseudomonas aeruginosa. Microb Pathog 2014; 74:25-32; PMID:25088031; http://dx.doi.org/10.1016/j.micpath.2014.07.008
   PubMed Web of Science ®Google Scholar
• Alatraktchi FA, Andersen SB, Johansen HK, Molin S, Svendsen WE. Fast Selective Detection of Pyocyanin Using Cyclic Voltammetry. Sensors (Basel) 2016; 16; PMID:27007376; http://dx.doi.org/10.3390/s16030408
   PubMed Web of Science ®Google Scholar
• Kessler E, Safrin M. Elastinolytic and proteolytic enzymes. Filloux A, and Ramos J. L. 135-69. 2014. Humana Press. Pseudomonas. Methods and Protocols
   Google Scholar
• Ohman DE, Cryz SJ, Iglewski BH. Isolation and characterization of Pseudomonas aeruginosa PAO mutant that produces altered elastase. J Bacteriol 1980; 142:836-42; PMID:6769912
   PubMed Web of Science ®Google Scholar
• Kessler E, Safrin M, Abrams WR, Rosenbloom J, Ohman DE. Inhibitors and specificity of Pseudomonas aeruginosa LasA. J Biol Chem 1997; 272:9884-9; PMID:9092525; http://dx.doi.org/10.1074/jbc.272.15.9884
   PubMed Web of Science ®Google Scholar
• Hamood AN, Griswold J, Colmer J. Characterization of elastase-deficient clinical isolates of Pseudomonas aeruginosa. Infect Immun 1996; 64:3154-60; PMID:8757847
   PubMed Web of Science ®Google Scholar
• Kashid SG, Ghosh JS. Production, Isolation and Characterization of Exotoxin Produced by Bacillus cereus NCIM-2156 and Bacillus licheniformis NCIM-5343. Br J Pharmacol Toxicol 2010; 1:50-5
   Google Scholar
• Ha D, Kuchma SL, O'Toole GA. Plate-Based assay for swimming motility in Pseudomonas aeruginosa. Filloux A, and Ramos J. L. 59-65. 2014. Humana Press. Pseudomonas. Methods and Protocols
   Google Scholar
• Ha D, Kuchma SL, O'Toole GA. Plate-Based assay for swarming motility in Pseudomonas aeruginosa. Filloux A, and Ramos J. L. 67-72. 2014. Humana Press. Pseudomonas. Methods and Protocols
   Google Scholar
• Turnbull L, Whitchurch CB. Motility assay: twitching motility. Filloux A, and Ramos J. L. 73-86. 2014. Humana Press. Pseudomonas. Methods and Protocols
   Google Scholar
• Thornsberry C. Antimicrobial susceptibility testing of anaerobic bacteria: review, comments, and opinions. Ann Otol Rhinol Laryngol Suppl 1991; 154:7-10; PMID:1952686
   PubMedGoogle Scholar
• Cumming G, Fidler F, Vaux DL. Error bars in experimental biology. J Cell Biol 2007; 177:7-11; PMID:17420288; http://dx.doi.org/10.1083/jcb.200611141
   PubMed Web of Science ®Google Scholar
• Bellin DL, Sakhtah H, Rosenstein JK, Levine PM, Thimot J, Emmett K, Dietrich LE, Shepard KL. Integrated circuit-based electrochemical sensor for spatially resolved detection of redox-active metabolites in biofilms. Nat Commun 2014; 5:3256; PMID:24510163; http://dx.doi.org/10.1038/ncomms4256
   PubMed Web of Science ®Google Scholar
• Bucior I, Pielage JF, Engel JN. Pseudomonas aeruginosa pili and flagella mediate distinct binding and signaling events at the apical and basolateral surface of airway epithelium. PLoS Pathog 2012; 8:e1002616; PMID:22496644; http://dx.doi.org/10.1371/journal.ppat.1002616
   PubMed Web of Science ®Google Scholar
• Ramphal R, Balloy V, Jyot J, Verma A, Si-Tahar M, Chignard M. Control of Pseudomonas aeruginosa in the lung requires the recognition of either lipopolysaccharide or flagellin. J Immunol 2008; 181:586-92; PMID:18566425; http://dx.doi.org/10.4049/jimmunol.181.1.586
   PubMed Web of Science ®Google Scholar