Wza gene knockout decreases Acinetobacter baumannii virulence and affects Wzy-dependent capsular polysaccharide synthesis

References

• Higgins PG, Dammhayn C, Hackel M, et al. Global spread of carbapenem-resistant Acinetobacter baumannii. J Antimicrob Chemother. 2010;65:233–238.
   PubMed Web of Science ®Google Scholar
• Boucher HW, Talbot GH, Bradley JS, et al. Bad bugs, no drugs: no ESKAPE! An update from the infectious diseases Society of America. Clin Infect Dis. 2009;48:1–12.
   PubMed Web of Science ®Google Scholar
• Jacobs AC, Thompson MG, Black CC, et al. AB5075, a highly virulent isolate of Acinetobacter baumannii, as a model strain for the evaluation of pathogenesis and antimicrobial treatments. MBio. 2014 May 27;5(3):e01076–14.
   PubMed Web of Science ®Google Scholar
• Harris G, Kuo Lee R, Lam CK, et al. A mouse model of Acinetobacter baumannii-associated pneumonia using a clinically isolated hypervirulent strain. Antimicrob Agents Chemother. 2013;57:3601–3613.
   PubMed Web of Science ®Google Scholar
• Jones CL, Clancy M, Honnold C, et al. Fatal outbreak of an emerging clone of extensively drug-resistant Acinetobacter baumannii with enhanced virulence. Clin Infect Dis. 2015;61:145–154.
   PubMed Web of Science ®Google Scholar
• Liu D, Liu ZS, Hu P, et al. Characterization of surface antigen protein 1 (SurA1) from Acinetobacter baumannii and its role in virulence and fitness. Vet Microbiol. 2016;186:126–138.
   PubMed Web of Science ®Google Scholar
• Choi CH, Lee JS, Lee YC, et al. Acinetobacter baumannii invades epithelial cells and outer membrane protein A mediates interactions with epithelial cells. BMC Microbiol. 2008;8:216.
   PubMed Web of Science ®Google Scholar
• Lee JS, Choi CH, Kim JW, et al. Acinetobacter baumannii outer membrane protein A induces dendritic cell death through mitochondrial targeting. J Microbiol. 2010 Jun;48(3):387–392.
   PubMed Web of Science ®Google Scholar
• Jacobs AC, Hood I, Boyd KL, et al. Inactivation of phospholipase D diminishes Acinetobacter baumannii pathogenesis. Infect Immun. 2010 May;78(5):1952–1962.
   PubMed Web of Science ®Google Scholar
• Geisinger E, Isberg RR. Antibiotic modulation of capsular exopolysaccharide and virulence in Acinetobacter baumannii. PLoS Pathog. 2015;11:e1004691.
   PubMed Web of Science ®Google Scholar
• Brossard KA, Campagnari AA. The Acinetobacter baumannii biofilm-associated protein plays a role in adherence to human epithelial cells. Infect Immun. 2012;80:228–233.
   PubMed Web of Science ®Google Scholar
• Iwashkiw JA, Seper A, Weber BS, et al. Identification of a general O-linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation. PLoS Pathog. 2012;8:e1002758.
   PubMed Web of Science ®Google Scholar
• Bentancor LV, Camacho-Peiro A, Cagla Bozkurt-Guzel GB, et al. Identification of Ata, a multifunctional trimeric autotransporter of Acinetobacter baumannii. J Bacteriol. 2012;194:3950–3960.
   PubMed Web of Science ®Google Scholar
• Tomaras AP, Dorsey CW, Edelmann RE, et al. Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chaperone-usher pili assembly system. Microbiology. 2003;149:3473–3484.
   PubMed Web of Science ®Google Scholar
• Gaddy JA, Arivett BA, McConnell MJ, et al. Role of acinetobactin-mediated iron acquisition functions in the interaction of Acinetobacter baumannii strain ATCC 19606T with human lung epithelial cells, Galleria mellonella caterpillars, and mice. Infect Immun. 2012;80(3):1015–1024.
   PubMed Web of Science ®Google Scholar
• King LB, Pangburn MK, McDaniel LS. Serine protease PKF of Acinetobacter baumannii results in serum resistance and suppression of biofilm formation. J Infect Dis. 2013 Apr;207(7):1128–1134.
   PubMed Web of Science ®Google Scholar
• Harding CM, Hennon SW, Feldman MF. Uncovering the mechanisms of Acinetobacter baumannii virulence. Nat Rev Microbiol. 2018;16:91–102.
   PubMed Web of Science ®Google Scholar
• Russo TA, Luke NR, Beanan JM, et al. The K1 capsular polysaccharide of Acinetobacter baumannii strain 307-0294 is a major virulence factor. Infect Immun. 2010;78:3993–4000.
   PubMed Web of Science ®Google Scholar
• Kenyon JJ, Hall RM. Variation in the complex carbohydrate biosynthesis loci of Acinetobacter baumannii genomes. PLoS One. 2013 Apr 16;8(4):e62160.
   PubMed Web of Science ®Google Scholar
• Hu D, Liu B, Dijkshoorn L, et al. Diversity in the major polysaccharide antigen of Acinetobacter baumannii assessed by DNA sequencing, and development of a molecular serotyping scheme. PLoS One. 2013;8(7):e70329.
   PubMed Web of Science ®Google Scholar
• Dong C, Beis K, Jutta Nesper AL, et al. The structure of Wza, the translocon for group 1 capsular polysaccharides in Escherichia coli, identifies a new class of outer membrane protein. Nature. 2006;444:226–229.
   PubMed Web of Science ®Google Scholar
• Drummelsmith J, Whitfield C. Translocation of group 1 capsular polysaccharide to the surface of Escherichia coli requires a multimeric complex in the outer membrane. Embo J. 2000;19:57–66.
   PubMed Web of Science ®Google Scholar
• Lin CL, Chen FH, Huang LY, et al. Effect in virulence of switching conserved homologous capsular polysaccharide genes from Klebsiella pneumoniae serotype K1 into K20. Virulence. 2017;8:487–493.
   PubMed Web of Science ®Google Scholar
• Yi H, Yuan B, Liu J, et al. Identification of a wza-like gene involved in capsule biosynthesis, pathogenicity and biofilm formation in Riemerella anatipestifer. Microb Pathog. 2017;107:442–450.
   PubMed Web of Science ®Google Scholar
• Hernández-Robles MF, Álvarez-Contreras AK, Juárez-García P, et al. Virulence factors and antimicrobial resistance in environmental strains of Vibrio alginolyticus. Int Microbiol. 2016;19:191–198.
   PubMed Web of Science ®Google Scholar
• Skerniškytė J, Krasauskas R, Péchoux C, et al. Surface-related features and virulence among Acinetobacter baumannii clinical isolates belonging to international clones I and II. Front Microbiol. 2019;9:3116.
   PubMed Web of Science ®Google Scholar
• Zhou K, Tang X, Wang LX, et al. An emerging clone (ST457) of Acinetobacter baumannii Clonal Complex 92 with enhanced virulence and increasing endemicity in South China. Clinl Infect Dis. 2018;67:S179–S188.
   PubMed Web of Science ®Google Scholar
• Liu X, Ji L, Wang X, et al. Role of RpoS in stress resistance, quorum sensing and spoilage potential of Pseudomonas fluorescens. Int J Food Microbiol. 2018 Apr 2;270:31–38.
   PubMed Web of Science ®Google Scholar
• Jie MIN, Kaiyu WANG, Tao LIU, et al. Construction and identification of invF gene deleted Yersinia ruckeri and its biological characteristics. J Fish China. 2017;41(12):1858–1866.
   Google Scholar
• Chen Z, Wang L, Zhang Y, et al. Establishment of a method for gene complementation in Vibrio parahaemolyticus. Nan Fang Yi Ke Da Xue Xue Bao. 2014 Jan;34(1):70–74.
   PubMedGoogle Scholar
• Hua Y, Sun Q, Wang X, et al. Construction of enterohemorrhagic Escherichia coli O157: h7strains with espF gene deletion and complementation. Nan Fang Yi Ke Da Xue Xue Bao. 2015 Nov;35(11):1546–1551.
   PubMedGoogle Scholar
• Yu DJ, Yu YS, Fang X, et al. Establishment of mice pneumonla model with imipenem-resistant Acinetobacter baumannii. China J Lab Med. 2010;33:771–775.
   Google Scholar
• Chang Y, Luan G, Ying X, et al. Characterization of carbapenem-resistant Acinetobacter baumannii isolates in a Chinese teaching hospital. Front Microbiol. 2015;6:910.
   PubMed Web of Science ®Google Scholar
• Ali HM, Salem MZM, El-Shikh MS, et al. Investigation of the virulence factors and molecular characterization of the clonal relations of multidrug-resistant acinetobacter baumannii isolates. J AOAC Int. 2017;100:152–158.
   PubMed Web of Science ®Google Scholar
• Giannouli M, Antunes LC, Marchetti V, et al. Virulence-related traits of epidemic Acinetobacter baumannii strains belonging to the international clonal lineages I-III and to the emerging genotypes ST25 and ST78. BMC Infect Dis. 2013;13:282.
   PubMed Web of Science ®Google Scholar
• Brazeau C, Gottschalk M, Vincelette S, et al. In vitro phagocytosis and survival of Streptococcus suis capsular type 2 inside murine macrophages[J]. Microbiology. 1996;142:1231–1237.
   PubMed Web of Science ®Google Scholar
• Costerton JW, Cheng KJ, Geesey GG, et al. Bacterial biofilms in nature and disease. Annu Rev Microbiol. 1987;41:435–464.
   PubMed Web of Science ®Google Scholar
• Tayabali AF, Nguyen KC, Shwed PS, et al. Comparison of the virulence potential of Acinetobacter strains from clinical and environmental sources. PLoS One. 2012;7:e37024.
   PubMed Web of Science ®Google Scholar
• Dertli E, Mayer MJ, Colquhoun IJ, et al. EpsA is an essential gene in exopolysaccharide production in Lactobacillus johnsonii FI9785. Microb Biotechnol. 2016;9:496–501.
   PubMed Web of Science ®Google Scholar
• Liu J, He D, Li XZ, et al. Gamma-polyglutamic acid (gamma-PGA) produced by Bacillus amyloliquefaciens C06 promoting its colonization on fruit surface. Int J Food Microbiol. 2010;142:190–197.
   PubMed Web of Science ®Google Scholar
• Islam ST, Lam JS. Synthesis of bacterial polysaccharides via the Wzx/Wzy-dependent pathway. Can J Microbiol. 2014;60(11):697–716.
   PubMed Web of Science ®Google Scholar
• Chakraborty AK, Friebolin H, Stirm S. Primary structure of the Escherichia coli serotype K30 capsular polysaccharide. J Bacteriol. 1980;141:971–972.
   PubMed Web of Science ®Google Scholar
• Collins RF, Konstantinos Beis BR, Clarke RC, et al. Periplasmic protein-protein contacts in the inner membrane protein Wzc form a tetrameric complex required for the assembly of Escherichia coli group 1 capsules. J Biol Chem. 2006;281:2144–2150.
   PubMed Web of Science ®Google Scholar
• Wugeditsch T, Paiment A, Hocking J, et al. Phosphorylation of Wzc, a tyrosine autokinase, is essential for assembly of group 1 capsular polysaccharides in Escherichia coli. J Biol Chem. 2001;276:2361–2371.
   PubMed Web of Science ®Google Scholar
• Paiment A, Hocking J, Whitfield C. Impact of phosphorylation of specific residues in the tyrosine autokinase, Wzc, on its activity in assembly of group 1 capsules in Escherichia coli. J Bacteriol. 2002;184:6437–6447.
   PubMed Web of Science ®Google Scholar
• Drummelsmith J, Whitfield C. Translocation of group 1 capsular polysaccharide to the surface of Escherichia coli requires a multimeric complex in the outer membrane. EMBO J. 2000;19:57–66.
   PubMed Web of Science ®Google Scholar
• Nesper J, Hill CM, Paiment A, et al. Translocation of group 1 capsular polysaccharide in Escherichia coli serotype K30. Structural and functional analysis of the outer membrane lipoprotein Wza. J Biol Chem. 2003;278:49763–49772.
   PubMed Web of Science ®Google Scholar
• Beis K, Collins RF, Ford RC, et al. Three-dimensional structure of Wza, the protein required for translocation of group 1 capsular polysaccharide across the outer membrane of Escherichia coli. J Biol Chem. 2004;279:28227–28232.
   PubMed Web of Science ®Google Scholar
• Hagelueken G, Huang H, Mainprize IL, et al. Crystal structures of Wzb of Escherichia coli and CpsB of Streptococcus pneumoniae, representatives of two families of tyrosine phosphatases that regulate capsule assembly. J Mol Biol. 2009;392:678–688.
   PubMed Web of Science ®Google Scholar
• Wugeditsch T, Paiment A, Hocking J, et al. Phosphorylation of Wzc, a tyrosine autokinase, is essential for assembly of group 1 capsular polysaccharides in Escherichia coli. J Biol Chem. 2001;276:2361–2371.
   PubMed Web of Science ®Google Scholar
• Collins RF, Beis K, Dong C, et al. The 3D structure of a periplasm-spanning platform required for assembly of group 1 capsular polysaccharides in Escherichia coli. Proc Natl Acad Sci U S A. 2007;104:2390–2395.
   PubMed Web of Science ®Google Scholar
• Reid AN, Whitfield C. Functional analysis of conserved gene products involved in assembly of Escherichia coli capsules and exopolysaccharides: evidence for molecular recognition between Wza and Wzc for colanic acid biosynthesis. J Bacteriol. 2005;187:5470–5481.
   PubMed Web of Science ®Google Scholar
• Bushell SR, Mainprize IL, Wear MA, et al. Wzi is an outer membrane lectin that underpins group 1 capsule assembly in Escherichia coli. Structure. 2013;21:844–853.
   PubMed Web of Science ®Google Scholar
• Dou H, Jiang M, Peng H, et al. pH-dependent self-assembly: micellization and micelle-hollow-sphere transition of cellulose-based copolymers. Angew Chem Int Ed Engl. 2003;42:1516–1519.
   PubMed Web of Science ®Google Scholar
• Long LX, Yuan XB, Chang J, et al. Self-assembly of polylactic acid and cholesterol-modified dextran into hollow nanocapsules. Carbohydr Polym. 2012;87:2630–2637.
   Web of Science ®Google Scholar
• Demarre G, Guérout AM, Matsumoto-Mashimo C, et al. A new family of mobilizable suicide plasmids based on broad host range R388 plasmid (IncW) and RP4 plasmid (IncPα) conjugative machineries and their cognate Escherichia coli host strains. Res Microbiol. 2005;156:245–255.
   PubMed Web of Science ®Google Scholar
• Luo P, He X, Liu Q, et al. Developing universal genetic tools for rapid and efficient deletion mutation in vibrio species based on suicide T-vectors carrying a novel counterselectable marker, vmi480. PLoS One. 2015;10:e0144465.
   PubMed Web of Science ®Google Scholar
• Hua Y, Sun Q, Wang X, et al. Construction of enterohemorrhagic Escherichia coli O157: h7strains with espF gene deletion and complementation. Nan Fang Yi Ke Da Xue Xue Bao. 2015;35:1546–1551.
   PubMedGoogle Scholar