Advanced search
Publication Cover
Virulence Volume 11, 2020 - Issue 1
Submit an article Journal homepage
3,073
Views
7
CrossRef citations to date
0
Altmetric
Research paper

Development of a fully automated high throughput PCR for the detection of SARS-CoV-2: The need for speed

Florian J. Mayera Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, AustriaView further author information
,
Franz Ratzingerb Ihr Labor, Medical Diagnostic Laboratories, Vienna, AustriaView further author information
,
Ralf L.J. Schmidta Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, AustriaView further author information
,
Georg Greinera Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, AustriaORCID Iconhttps://orcid.org/0000-0002-0917-4117View further author information
ORCID Icon,
Olfert Landtc Tib-Molbiol, Berlin, GermanyView further author information
,
Alexander Am Endec Tib-Molbiol, Berlin, GermanyView further author information
,
Victor M. Cormand Charité – Universitätsmedizin Berlin Institute of Virology, Berlin, Germany and German Centre for Infection Research (DZIF), Berlin, GermanyORCID Iconhttps://orcid.org/0000-0002-3605-0136View further author information
ORCID Icon,
Nicole Perkmann-Nagelea Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, AustriaView further author information
,
Thomas Watkins-Riedela Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, AustriaView further author information
,
Dagmar Petermanna Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, AustriaView further author information
,
Karoline Abadire QIAGEN GmbH, Vienna, AustriaView further author information
,
Josef Zweimüller-Mayere QIAGEN GmbH, Vienna, AustriaView further author information
&
Robert Strassla Divison of Clinical Virology, Department of Laboratory Medicine, Medical University of Vienna, Vienna, AustriaCorrespondence[email protected]
View further author information
 show all
Pages 964-967 | Received 24 Mar 2020, Accepted 30 Jun 2020, Published online: 29 Jul 2020

References

  • Zhu N, Zhang D, Wang W, et al. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382(8):727–733.
  • Corman VM, Landt O, Kaiser M, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25:3.
  • Chu DKW, Pan Y, Cheng SMS, et al. Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia. Clin Chem. 2020;66(4):549–555.
  • Reusken C, Broberg EK, Haagmans B, et al. Laboratory readiness and response for novel coronavirus (2019-nCoV) in expert laboratories in 30 EU/EEA countries, January 2020. Euro Surveill. 2020;25:6.
  • Bai Y, Yao L, Wei T, et al. Presumed asymptomatic carrier transmission of COVID-19. JAMA. 2020;323(14):1406.
  • Zou L, Ruan F, Huang M, et al. SARS-CoV-2 viral load in upper respiratory specimens of infected patients. N Engl J Med. 2020;382(12):1177–1179.
  • Wilke WW, Jones RN, Sutton LD. Automation of polymerase chain reaction tests. Reduction of human errors leading to contamination. Diagn Microbiol Infect Dis. 1995;21(4):181–185.
  • MacLean AR, Gunson R. Automation and standardisation of clinical molecular testing using PCR.Ai – A comparative performance study. J Clin Virol. 2019;120:51–56.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.