Mitochondrial transformation occurs in cultured adipocytes, but fails to increase adipose tissue metabolic activity in mice in vivo

References

• Cinti S. The adipose organ. Prostaglandins Leukot Essent Fatty Acids. 2005;73:9–15.
   PubMed Web of Science ®Google Scholar
• Cannon B, Nedergaard J. Brown adipose tissue: function and physiological significance. Physiol Rev. 2004;84:277–359.
   PubMed Web of Science ®Google Scholar
• Frontini A, et al. White-to-brown transdifferentiation of omental adipocytes in patients affected by pheochromocytoma. Biochim Biophys Acta. 2013;1831:950–959.
   PubMed Web of Science ®Google Scholar
• Wu J, et al. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell. 2012;150:366–376.
   PubMed Web of Science ®Google Scholar
• Rosenwald M, Perdikari A, Rülicke T, et al. Bi-directional interconversion of brite and white adipocytes. Nat Cell Bio. 2013;15:659–667.
   PubMed Web of Science ®Google Scholar
• Rosenwald M, Wolfrum C. The origin and definition of brite versus white and classical brown adipocytes. Adipocyte. 2014;3:4–9.
   PubMedGoogle Scholar
• Calvo SE, Mootha VK. The mitochondrial proteome and human disease. Annu Rev Genomics Hum Genet. 2010;11:25–44.
   PubMed Web of Science ®Google Scholar
• Kim T-Y, et al. Metabolic labeling reveals proteome dynamics of mouse mitochondria. Mol Cell Proteomics MCP. 2012;11:1586–1594.
   PubMed Web of Science ®Google Scholar
• Choo H-J, et al. Mitochondria are impaired in the adipocytes of type 2 diabetic mice. Diabetologia. 2006;49:784–791.
   PubMed Web of Science ®Google Scholar
• Boden G, et al. Thiazolidinediones upregulate fatty acid uptake and oxidation in adipose tissue of diabetic patients. Diabetes. 2005;54:880–885.
   PubMed Web of Science ®Google Scholar
• Kesner EE, Saada-Reich A, Lorberboum-Galski H. Characteristics of mitochondrial transformation into human cells. Sci Rep. 2016;6:26057.
   PubMed Web of Science ®Google Scholar
• Masuzawa A, et al. Transplantation of autologously derived mitochondria protects the heart from ischemia-reperfusion injury. Am J Physiol Heart Circ Physiol. 2013;304:H966–982.
   PubMed Web of Science ®Google Scholar
• Kaza AK, et al. Myocardial rescue with autologous mitochondrial transplantation in a porcine model of ischemia/reperfusion. J Thorac Cardiovasc Surg. 2017;153:934–943.
   PubMed Web of Science ®Google Scholar
• Puigserver P, et al. Induction and degradation of the uncoupling protein thermogenin in brown adipocytes in vitro and in vivo. Evidence for a rapidly degradable pool. Biochem J. 1992;284(Pt 2):393–398.
   PubMedGoogle Scholar
• Clark MA, Shay JW. Mitochondrial transformation of mammalian cells. Nature. 1982;295:605–607.
   PubMed Web of Science ®Google Scholar
• Emani SM, Piekarski BL, Harrild D, et al. Autologous mitochondrial transplantation for dysfunction after ischemia-reperfusion injury. J Thorac Cardiovasc Surg. 2017;154:286–289.
   PubMed Web of Science ®Google Scholar
• Hammel JM. Mitochondrial autotransplantation: a ‘shot’ in the dark? J Thorac Cardiovasc Surg. 2017;154:290.
   PubMed Web of Science ®Google Scholar
• Bertero E, Maack C, O’Rourke B. Mitochondrial transplantation in humans: “magical” cure or cause for concern? J Clin Invest. 2018;128:5191–5194.
   PubMed Web of Science ®Google Scholar
• Katrangi E, et al. Xenogenic transfer of isolated murine mitochondria into human rho0 cells can improve respiratory function. Rejuvenation Res. 2007;10:561–570.
   PubMed Web of Science ®Google Scholar
• Pacak CA, et al. Actin-dependent mitochondrial internalization in cardiomyocytes: evidence for rescue of mitochondrial function. Biol Open. 2015;4:622–626.
   PubMed Web of Science ®Google Scholar
• Miwa S, Lawless C, von Zglinicki T. Mitochondrial turnover in liver is fast in vivo and is accelerated by dietary restriction: application of a simple dynamic model. Aging Cell. 2008;7:920–923.
   PubMed Web of Science ®Google Scholar
• Menzies RA, Gold PH. The turnover of mitochondria in a variety of tissues of young adult and aged rats. J Biol Chem. 1971;246:2425–2429.
   PubMed Web of Science ®Google Scholar
• Gunter TE, Pfeiffer DR. Mechanisms by which mitochondria transport calcium. Am J Physiol. 1990;258:C755–786.
   PubMed Web of Science ®Google Scholar
• Preble JM, et al. Rapid isolation and purification of mitochondria for transplantation by tissue dissociation and differential filtration. J Vis Exp. 2014;e51682. DOI:10.3791/51682.
   Google Scholar
• McCully JD, et al. Injection of isolated mitochondria during early reperfusion for cardioprotection. Am J Physiol Heart Circ Physiol. 2009;296:H94–H105.
   PubMed Web of Science ®Google Scholar
• Cowan DB, et al. Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection. PloS One. 2016;11:e0160889.
   PubMed Web of Science ®Google Scholar
• Elabd C, et al. Human multipotent adipose-derived stem cells differentiate into functional brown adipocytes. Stem Cells Dayt Ohio. 2009;27:2753–2760.
   PubMed Web of Science ®Google Scholar
• Klein J, Fasshauer M, Klein HH, et al. Novel adipocyte lines from brown fat: a model system for the study of differentiation, energy metabolism, and insulin action. BioEssays News Rev Mol Cell Dev Biol. 2002;24:382–388.
   Google Scholar