An optimized method for Oil Red O staining with the salicylic acid ethanol solution

References

• Bharati S, Anjaly K, Thoidingjam S, et al. Oil Red O based method for exosome labelling and detection. Biochem Biophys Res Commun. 2022 Jun;611(611):179–10.
   PubMedGoogle Scholar
• Ramirez-Zacarias JL, Castro-Munozledo F, Kuri-Harcuch W. Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry. 1992 Jul;97(6):493–497.
   PubMedGoogle Scholar
• Mehlem A, Hagberg CE, Muhl L, et al. Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease. Nat Protoc. 2013 Jun;8(6):1149–1154.
   PubMed Web of Science ®Google Scholar
• Koopman R, Schaart G, Hesselink MK. Optimisation of oil red O staining permits combination with immunofluorescence and automated quantification of lipids. Histochem Cell Biol. 2001 Jul;116(1):63–68.
   PubMed Web of Science ®Google Scholar
• Levene AP, Kudo H, Armstrong MJ, et al. Quantifying hepatic steatosis - more than meets the eye. Histopathology. 2012 May;60(6):971–981.
   PubMed Web of Science ®Google Scholar
• Catta-Preta M, Mendonca LS, Fraulob-Aquino J, et al. A critical analysis of three quantitative methods of assessment of hepatic steatosis in liver biopsies. Virchows Arch. 2011 Nov;459(5):477–485.
   PubMed Web of Science ®Google Scholar
• Fukumoto S, Fujimoto T. Deformation of lipid droplets in fixed samples. Histochem Cell Biol. 2002 Nov;118(5):423–428.
   PubMed Web of Science ®Google Scholar
• Bumbrah GS, Sodhi GS, Oil Red KJ. O (ORO) reagent for detection of latent fingermarks: a review. Egypt J Forensic Sci. 2019;9:1.
   Web of Science ®Google Scholar
• Spangenburg EE, Pratt SJP, Wohlers LM, et al. Use of BODIPY (493/503) to visualize intramuscular lipid droplets in skeletal muscle. J Biomed Biotechnol. 2011;2011:598358.
   PubMedGoogle Scholar
• Kinkel AD, Fernyhough ME, Helterline DL, et al. Oil red-O stains non-adipogenic cells: a precautionary note. Cytotechnology. 2004 Sep;46(1):49–56.
   PubMed Web of Science ®Google Scholar
• Kapp, Jr RW, Bevan C, Gardiner TH, et al. Isopropanol: summary of TSCA test rule studies and relevance to hazard identification. Regul Toxicol Pharmacol. 1996;23(3):183–192.
   PubMed Web of Science ®Google Scholar
• Slaughter RJ, Mason RW, Beasley DM, et al. Isopropanol poisoning. Clin Toxicol (Phila). 2014 Jun;52(5):470–478.
   PubMed Web of Science ®Google Scholar
• Chang T-S. An updated review of tyrosinase inhibitors. Int J Mol Sci. 2009 May 26;10(6):2440–2475.
   PubMed Web of Science ®Google Scholar
• Bandara M, Sankaridurg P, Zhu H, et al. Effect of salicylic acid on the membrane proteome and virulence of Pseudomonas aeruginosa. Invest Ophthalmol Vis Sci. 2016 Mar;57(3):1213–1220.
   PubMed Web of Science ®Google Scholar
• Lee KC, Wambier CG, Soon SL, et al. Basic chemical peeling: superficial and medium-depth peels. J Am Acad Dermatol. 2019 Aug;81(2):313–324.
   PubMed Web of Science ®Google Scholar
• Kraus NA, Ehebauer F, Zapp B, et al. Quantitative assessment of adipocyte differentiation in cell culture. Adipocyte. 2016;5(4):351–358.
   PubMed Web of Science ®Google Scholar
• Wang QM, Huang XY, Guan WQ. Expressions of Interleukin-27 in oral lichen planus, oral leukoplakia, and oral squamous cell carcinoma. Inflammation. 2022 Jun;45(3):1023–1038.
   PubMed Web of Science ®Google Scholar
• Jun I, Kim BR, Park SY, et al. Interleukin-4 stimulates lipogenesis in meibocytes by activating the STAT6/PPARgamma signaling pathway. Ocul Surf. 2020 Oct;18(4):575–582.
   PubMed Web of Science ®Google Scholar
• Fowler SD, Greenspan P. Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with oil red O. J Histochem Cytochem. 1985;33(8):833–836.
   PubMed Web of Science ®Google Scholar
• Patel F, Parwani K, Patel D, et al. Metformin and probiotics interplay in amelioration of ethanol-induced oxidative stress and inflammatory response in an in vitro and in vivo model of hepatic injury. Mediators Inflamm. 2021;2021:6636152.
   PubMed Web of Science ®Google Scholar
• Salama J, Donovan A, Lennard C, et al. Evaluation of the fingermark reagent oil red O as a possible replacement for physical developer. J Forensic Identif. 2008;58:2.
   Google Scholar
• Madan RK, Levitt J. A review of toxicity from topical salicylic acid preparations. J Am Acad Dermatol. 2014 Apr;70(4):788–792.
   PubMed Web of Science ®Google Scholar
• Arif T. Salicylic acid as a peeling agent: a comprehensive review. Clin Cosmet Investig Dermatol. 2015;8:455–461.
   PubMed Web of Science ®Google Scholar
• Chiara C, Grazia CM, Elena C, et al. Antiinflammatory action of salicylates: aspirin is not a prodrug for salicylate against rat carrageenin pleurisy. Eur J Pharmacol. 1989;159:3.
   Web of Science ®Google Scholar
• Dainichi T, Ueda S, Imayama S, et al. Excellent clinical results with a new preparation for chemical peeling in acne: 30% salicylic acid in polyethylene glycol vehicle. Dermatol Surg. 2008 Jul;34(7):891–899. discussion 899
   PubMed Web of Science ®Google Scholar
• Bae BG, Park CO, Shin H, et al. Salicylic acid peels versus Jessner’s solution for acne vulgaris: a comparative study. Dermatol Surg. 2013 Feb;39(2):248–253.
   PubMed Web of Science ®Google Scholar
• Lu J, Cong T, Wen X, et al. Salicylic acid treats acne vulgaris by suppressing AMPK/SREBP1 pathway in sebocytes. Exp Dermatol. 2019 Jul;28(7):786–794.
   PubMed Web of Science ®Google Scholar
• Zhu G, Xia Y, Zhao Z, et al. LncRNA XIST from the bone marrow mesenchymal stem cell derived exosome promotes osteosarcoma growth and metastasis through miR-655/ACLY signal. Cancer Cell Int. 2022 Oct 29;22(1):330.
   PubMed Web of Science ®Google Scholar
• Diascro DD Jr., Vogel RL, Johnson TE, et al. High fatty acid content in rabbit serum is responsible for the differentiation of osteoblasts into adipocyte-like cells. J Bone Miner Res. 1998 Jan;13(1):96–106.
   PubMed Web of Science ®Google Scholar
• Huang H, Liu Y, Wang L, et al. Age-related macular degeneration phenotypes are associated with increased tumor necrosis-alpha and subretinal immune cells in aged Cxcr5 knockout mice. PLoS One. 2017;12(3):e0173716.
   PubMed Web of Science ®Google Scholar
• Hachana S, Pouliot M, Couture R, et al. Diabetes-induced inflammation and vascular alterations in the goto-kakizaki rat retina. Curr Eye Res. 2020 Aug;45(8):965–974.
   PubMed Web of Science ®Google Scholar
• Luo X, Li H, Ma L, et al. Expression of STING is increased in liver tissues from patients with NAFLD and promotes macrophage-mediated hepatic inflammation and fibrosis in mice. Gastroenterology. 2018 Dec;155(6):1971–1984 e4.
   PubMed Web of Science ®Google Scholar
• Dias HB, Krause GC, Squizani ED, et al. Fructose-1,6-bisphosphate reverts iron-induced phenotype of hepatic stellate cells by chelating ferrous ions. Biometals. 2017 Aug;30(4):549–558.
   PubMed Web of Science ®Google Scholar
• Song Z, Cheng J, Yang H, et al. Differentiation of 3T3-L1 preadipocytes is inhibited under a modified ceiling culture. Cell Biol Int. 2015 May;39(5):638–645.
   PubMed Web of Science ®Google Scholar
• Du J, Cao X, Diao J, et al. Neonatal overfeeding in mice aggravates the development of methionine and choline-deficient diet-induced steatohepatitis in adulthood. Genes Dis. 2019 Mar;6(1):68–77.
   PubMed Web of Science ®Google Scholar