Prodigiosin: a promising biomolecule with many potential biomedical applications

References

• Koyande AK, Show PL, Guo R, et al. Bio-processing of algal bio-refinery: a review on current advances and future perspectives. Bioengineered. 2019;10(1):574–592.
   PubMed Web of Science ®Google Scholar
• Numan M, Bashir S, Mumtaz R, et al. Therapeutic applications of bacterial pigments: a review of current status and future opportunities. 3 Biotech. 2018;8(4).
   PubMedGoogle Scholar
• Venil C. An insightful overview on microbial pigment, prodigiosin. Electron J. 2009;5:49–61.
   Google Scholar
• Williamson NR, Fineran PC, Leeper FJ, et al. The biosynthesis and regulation of bacterial prodiginines. Nat Rev Microbiol. 2006;4(12):887–899.
   PubMed Web of Science ®Google Scholar
• Venil CK, Dufossé L, Renuka Devi P. Bacterial pigments: sustainable compounds with market potential for pharma and food industry, Front Sustain Food Syst. 2020;4(1):49–61
   Google Scholar
• Bernardes N, Seruca R, Chakrabarty AM, et al. Microbial-based therapy of cancer current progress and future prospects. Bioeng Bugs. 2010;1(3):178–190
   PubMedGoogle Scholar
• Durán N, Nakazato G, Durán M, et al. Multi-target drug with potential applications: violacein in the spotlight. World J Microbiol Biotechnol. 2021;37(9):1–20.
   Web of Science ®Google Scholar
• Han R, Xiang R, Li J, et al. High-level production of microbial prodigiosin: a review. J Bas Microbiol. 2021;61(6):506–523.
   PubMed Web of Science ®Google Scholar
• Darshan N, Manonmani HK. Prodigiosin and its potential applications. J Food Sci Technol. 2015;52(9):5393–5407.
   PubMed Web of Science ®Google Scholar
• Sudhakar C, Shobana C, Selvankumar T, et al. Prodigiosin production from Serratia marcescens strain CSK and their antioxidant, antibacterial, cytotoxic effect and in silico study of caspase-3 apoptotic protein. Biotechnol Appl Biochem. 2021. 10.1002/bab.2261
   PubMed Web of Science ®Google Scholar
• Suryawanshi RK, Patil CD, Koli SH, et al. Antimicrobial activity of prodigiosin is attributable to plasma-membrane damage. Nat Prod Res. 2017;31(5):572–577.
   PubMed Web of Science ®Google Scholar
• Yip CH, Mahalingam S, Wan KL, et al. Prodigiosin inhibits bacterial growth and virulence factors as a potential physiological response to interspecies competition. PLoS One. 2021;16(6):e0253445.
   PubMed Web of Science ®Google Scholar
• Kurz CL, Chauvet S, Andrès E, et al. Virulence factors of the human opportunistic pathogen Serratia marcescens identified by in vivo screening. EMBO J. 2003;22(7):1451–1460.
   PubMed Web of Science ®Google Scholar
• Wang SL, Wang SL, Nguyen VB, et al. Production and potential applications of bioconversion of chitin and protein-containing fishery byproducts into prodigiosin. Rev Mol. 2020;25.
   Google Scholar
• Paul T, Bandyopadhyay TK, Mondal A, et al. A comprehensive review on recent trends in production, purification, and applications of prodigiosin. Biomass Convers Bioref. 2022;12:1409–1431.
   Web of Science ®Google Scholar
• Jeong YJ, Kim HJ, Kim S, et al. Enhanced large-scale production of Hahella chejuensis -derived prodigiosin and evaluation of its bioactivity. J Microbiol Biotechnol. 2021;31(12):1624–1631.
   PubMed Web of Science ®Google Scholar
• Bennett JW, Bentley R. Seeing red: the story of prodigiosin. Adv Appl Microbiol. 2000;47:1–32.
   PubMed Web of Science ®Google Scholar
• Zang CZ, Yeh CW, Chang WF, et al. Identification and enhanced production of prodigiosin isoform pigment from Serratia marcescens N10612. J Taiwan Inst Chem Eng. 2014;45(4):1133–1139.
   Web of Science ®Google Scholar
• Gong J, Ren Y, Fu R, et al. pH-mediated antibacterial dyeing of cotton with prodigiosins nanomicelles produced by microbial fermentation. Polymers (Basel). 2017;9(12):468.
   PubMedGoogle Scholar
• Kulandaisamy Venil C, Lakshmanaperumalsamy P. An insightful overview on microbial pigment, prodigiosin. Electron J Biol. 2009;5:49–61.
   Google Scholar
• Brands S, Brass HUC, Klein AS, et al. KnowVolution of prodigiosin ligase pigc towards condensation of short-chain prodiginines. Catal Sci Technol. 2021;11(8):2805–2815.
   Web of Science ®Google Scholar
• Jia X, Liu F, Zhao K, et al. Identification of essential genes associated with prodigiosin production in Serratia marcescens FZSF02. Front Microbiol. 2021;12. 10.3389/fmicb.2021.705853
   Web of Science ®Google Scholar
• Yip CH, Yarkoni O, Ajioka J, et al. Recent advancements in high-level synthesis of the promising clinical drug, prodigiosin. Appl Microbiol Biotechnol. 2019;103(4):1667–1680.
   PubMed Web of Science ®Google Scholar
• Jehlička J, Edwards HGM, Oren A. Analysis of brown, violet and blue pigments of microorganisms by Raman spectroscopy. TrAC - Trends Anal Chem. 2022;146:116501.
   Web of Science ®Google Scholar
• Carbonell GV, Della Colleta HHM, Yano T, et al. Clinical relevance and virulence factors of pigmented Ssrratia marcescens. FEMS Immunol Med Microbiol. 2000;28:143–149.
   PubMed Web of Science ®Google Scholar
• Jissa G, Soorej M. Prodigiosin from marine bacterium production, characterization and application as dye in textile. Int J Biotechnol Biochem. 2011;7:155–191.
   Google Scholar
• Choi SY, Lim S, Hye YK, et al. Biotechnological activities and applications of bacterial pigments violacein and prodigiosin. J Biol Eng. 2021;15(1):1–16.
   PubMed Web of Science ®Google Scholar
• Gerber NN, Lechevalier MP. Prodiginine (prodigiosin like) pigments from streptomyces and other aerobic actinomycetes. Can J Microbiol. 1976;22:658–667.
   PubMed Web of Science ®Google Scholar
• Elahian F, Moghimi B, Dinmohammadi F, et al. The anticancer agent prodigiosin is not a multidrug resistance protein substrate. DNA Cell Biol. 2013;32(3):90–97.
   PubMed Web of Science ®Google Scholar
• Rastegari B, Karbalaei-Heidari HR. Sulfate as a pivotal factor in regulation of Serratia sp. strain S2B pigment biosynthesis. Res Microbiol. 2016;167(8):638–646.
   PubMed Web of Science ®Google Scholar
• Song MJ, Bae J, Lee DS, et al. Purification and characterization of prodigiosin produced by integrated bioreactor from Serratia sp KH-95. J Biosci Bioeng. 2006;101(2):157–161.
   PubMed Web of Science ®Google Scholar
• Elmenshawey A, Abdelrazak A, Mowafy AM, et al. Optimization of bioreactor cultivation parameters by taguchi orthogonal array design for enhanced prodigiosin production. Iran J Chem Chem Eng.2022 May 5; 39:319–330.
   Google Scholar
• Gondil VS, Asif M, Bhalla TC. Optimization of physicochemical parameters influencing the production of prodigiosin from Serratia nematodiphila RL2 and exploring its antibacterial activity. 3 Biotech. 2022 May 5; 7(5). 10.1007/s13205-017-0979-z
   Google Scholar
• Abdul Manas NH, Chong LY, Tesfamariam YM, et al. Effects of oil substrate supplementation on production of prodigiosin by Serratia nematodiphila for dye-sensitized solar cell. J Biotechnol. 2022 May 5; 317:16–26.
   Web of Science ®Google Scholar
• Metwally RA, El-Sersy NA, El Sikaily A, et al. Statistical optimization and characterization of prodigiosin from a marine Serratia rubidaea RAM-Alex. J Pure Appl Microbiol. 2017;11(3):1259–1266.
   Google Scholar
• El-Bondkly AMA, Mervat MMM, Bassyouni RH. Overproduction and biological activity of prodigiosin-like pigments from recombinant fusant of endophytic marine Streptomyces species. Antonie van leeuwenhoek. Int J Gen Mol Microbiol. 2022 May 5; 102:719–734.
   Google Scholar
• Stankovic N, Radulovic V, Petkovic M, et al. Nikodinovic-Runic J. Streptomyces sp. JS520 produces exceptionally high quantities of undecylprodigiosin with antibacterial, antioxidative, and UV-protective properties. Appl Microbiol Biotechnol. 2022 May 5; 96:1217–1231.
   Google Scholar
• Liu P, Zhu H, Zheng G, et al. Metabolic engineering of Streptomyces coelicolor for enhanced prodigiosins (RED) production. Sci China Life Sci. 2022 May 5; 60(9):948–957.
   Google Scholar
• Erandapurathukadumana Sreedharan H, Harilal CC, Pradeep S. Response surface optimization of prodigiosin production by phthalate degrading Achromobacter denitrificans SP1 and exploring its antibacterial activity. Prep Biochem Biotechnol. 2022 May 5; 50(6):564–571.
   Web of Science ®Google Scholar
• Lee JS, Kim YS, Park S, et al. Exceptional production of both prodigiosin and cycloprodigiosin as major metabolic constituents by a novel marine bacterium, Zooshikella rubidus S1-1. Appl Environ Microbiol. 2022 May 5; 77:4967–4973.
   Google Scholar
• Haddix PL, Shanks RMQ. Prodigiosin pigment of Serratia marcescens is associated with increased biomass production. Arch Microbiol. 2018;200(7):989–999.
   PubMed Web of Science ®Google Scholar
• Wang F, Luo H, Song G, et al. Prodigiosin found in Serratia marcescens y2 initiates phototoxicity in the cytomembrane. Electron J Biotechnol. 2013;16(4
   Web of Science ®Google Scholar
• Andreeva IN, Ogorodnikova TI. Pigmentation of Serratia marcescens and spectral properties of prodigiosin. Mikrobiologiia. 2015;84(1):43–49.
   PubMedGoogle Scholar
• Kurbanoglu EB, Ozdal M, Ozdal OG, et al. Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone. Brazilian J Microbiol. 2015;46(2):631–637.
   PubMed Web of Science ®Google Scholar
• Wei YH, Chen WC. Enhanced production of prodigiosin-like pigment from Serratia marcescens by medium improvement and oil-supplementation strategies. J Biosci Bioeng. 2005;99(6):616–622.
   PubMed Web of Science ®Google Scholar
• Sezonov G, Joseleau-Petit D, D’Ari R. Escherichia coli physiology in Luria-bertani broth. J Bacteriol. 2022 May 5; 189(23):8746–8749.
   Google Scholar
• Williams RP. Biosynthesis of prodigiosin, a secondary metabolite of Serratia marcescens. Appl Microbiol. 1973;25(3):396–402.
   PubMedGoogle Scholar
• Faraag AH A, El-Batal I, El-Hendawy HH. Characterization of prodigiosin produced by Serratia marcescens strain isolated from irrigation water in Egypt. Nat Sci. 2017;15(5). Available from. http://www.sciencepub.net/naturehttp://www.sciencepub.net/nature.8.doi:10.7537/marsnsj150517.08
   Google Scholar
• Tarafdar A, Gaur VK, Rawat N, et al. Advances in biomaterial production from animal derived waste. Bioengineered. 2021;12(1):8247–8258.
   PubMed Web of Science ®Google Scholar
• Aruldass CA, Venil CK, Zakaria ZA, et al. Brown sugar as a low-cost medium for the production of prodigiosin by locally isolated Serratia marcescens UTM1. Int Biodeterior Biodegrad. 2014;95:19–24.
   Web of Science ®Google Scholar
• Elkenawy NM, Yassin AS, Elhifnawy HN, et al. Optimization of prodigiosin production by Serratia marcescens using crude glycerol and enhancing production using gamma radiation. Biotechnol Reports. 2022 May 5; 14:47–53.
   Google Scholar
• Arslan NP. Use of wool protein hydrolysate as nitrogen source in production of microbial pigments. J Food Process Preserv. 2021;45(7):e15660.
   Web of Science ®Google Scholar
• Wang SL, Wang CY, Yen YH, et al. Enhanced production of insecticidal prodigiosin from Serratia marcescens TKU011 in media containing squid pen. Process Biochem. 2012;47(11):1684–1690.
   Web of Science ®Google Scholar
• Giri AV, Anandkumar N, Muthukumaran G, et al. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol. 2004;4(1):1–10.
   PubMedGoogle Scholar
• Nguyen TH, Wang SL, Doan MD, et al. Utilization of by-product of groundnut oil processing for production of prodigiosin by microbial fermentation and its novel potent anti-nematodes effect. Agronomy. 2022;12(1):41.
   Google Scholar
• De Araújo HWC, Fukushima K, Takaki GMC. Prodigiosin production by Serratia marcescens UCP 1549 using renewable-resources as a low cost substrate. Molecules. 2010;15(10):6931–6940.
   PubMed Web of Science ®Google Scholar
• Tran LT, Techato K, Nguyen VB, et al. Utilization of cassava wastewater for low-cost production of prodigiosin via Serratia marcescens tnu01 fermentation and its novel potent α-glucosidase inhibitory effect. Molecules. 2021;26(20):6270.
   PubMed Web of Science ®Google Scholar
• Dos Santos RA, Rodríguez DM, da Silva LAR, et al. Enhanced production of prodigiosin by Serratia marcescens UCP 1549 using agrosubstrates in solid-state fermentation. Arch Microbiol. 2021;203(7):4091–4100.
   PubMed Web of Science ®Google Scholar
• Chen WC, Yu WJ, Chang CC, et al. Enhancing production of prodigiosin from Serratia marcescens C3 by statistical experimental design and porous carrier addition strategy. Biochem Eng J. 2013;78:93–100.
   Web of Science ®Google Scholar
• Panesar R, Kaur S, Panesar PS. Production of microbial pigments utilizing agro-industrial waste: a review. Curr Opin Food Sci. 2022 May 5; 1:70–76.
   Google Scholar
• Lopes FC, Ligabue-Braun R. Agro-industrial residues: eco-friendly and inexpensive substrates for microbial pigments production. Front Sustain Food Syst. 2021;5:65.
   Google Scholar
• Williams RP, Gott CL, Qadri SM, et al. Influence of temperature of incubation and type of growth medium on pigmentation in Serratia marcescens. J Bacteriol. 1971;106(2):438–443.
   PubMed Web of Science ®Google Scholar
• Khanam B, Chandra R. Comparative analysis of prodigiosin isolated from endophyte Serratia marcescens. Lett Appl Microbiol. 2018;66(3):194–201.
   PubMed Web of Science ®Google Scholar
• Lin C, Jia X, Fang Y, et al. Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets. Electron J Biotechnol. 2019;40:58–64.
   Web of Science ®Google Scholar
• Ren Y, Gong J, Fu R, et al. Dyeing and functional properties of polyester fabric dyed with prodigiosins nanomicelles produced by microbial fermentation. J Clean Prod. 2017;148:375–385.
   Web of Science ®Google Scholar
• Chávez-Castilla LR, Aguilar O. An integrated process for the in situ recovery of prodigiosin using micellar ATPS from a culture of Serratia marcescens. J Chem Technol Biotechnol. 2016;91(11):2896–2903.
   Web of Science ®Google Scholar
• Luong VT, Thanh NS L, Tuyen DT, et al. Prodigiosin purification from Serratia marcescens M10 and its antitumor activities. Vietnam J Biotechnol. 2021;19(2):289–299
   Google Scholar
• Hu W, Zheng R, Liao Y, et al. Evaluating the biological potential of prodigiosin from Serratia marcescens kh-001 against asian citrus psyllid. J Econ Entomol. 2021;114(3):1219–1225.
   PubMed Web of Science ®Google Scholar
• Yang Q, Gao Y, Ke J, et al. Antibiotics: an overview on the environmental occurrence, toxicity, degradation, and removal methods. Bioengineered. 2021;12(1):7376–7416.
   PubMed Web of Science ®Google Scholar
• Lapenda JC, Silva PA, Vicalvi MC, et al. Antimicrobial activity of prodigiosin isolated from Serratia marcescens UFPEDA 398. World J Microbiol Biotechnol. 2015;31(2):399–406.
   PubMed Web of Science ®Google Scholar
• Arivizhivendhan KV, Mahesh M, Boopathy R, et al. Antioxidant and antimicrobial activity of bioactive prodigiosin produces from Serratia marcescens using agricultural waste as a substrate. J Food Sci Technol. 2018;55(7):2661–2670.
   PubMed Web of Science ®Google Scholar
• Lin SR, Chen YH, Tseng FJ, et al. The production and bioactivity of prodigiosin: quo vadis? Drug Discov Today. 2020;25(5):828–836.
   PubMed Web of Science ®Google Scholar
• Darshan N, Manonmani HK. Prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death. AMB Express. 2016;6(1). DOI:10.1186/s13568-016-0222-z
   PubMedGoogle Scholar
• Ravindran A, Anishetty S, Pennathur G. Molecular dynamics of the membrane interaction and localisation of prodigiosin. J Mol Graph Model. 2020;98:107614.
   PubMed Web of Science ®Google Scholar
• Danevčič T, Vezjak MB, Zorec M, et al. Prodigiosin - A multifaceted Escherichia coli antimicrobial agent. PLoS One. 2016;11(9):e0162412.
   PubMed Web of Science ®Google Scholar
• Montaner B, Castillo-Ávila W, Martinell M, et al. DNA interaction and dual topoisomerase I and II inhibition properties of the anti-tumor drug prodigiosin. Toxicol Sci. 2005;85(2):870–879.
   PubMed Web of Science ®Google Scholar
• Berlanga M, Ruiz N, Hernandez-Borrell J, et al. Role of the outer membrane in the accumulation of quinolones by Serratia marcescens. Can J Microbiol. 2000;46(8):716–722.
   PubMed Web of Science ®Google Scholar
• Suryavanshi MV, Waghmode SR, Bharti N, et al. Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia marcescens OX_R strain. J Appl Pharm Sci. 2016;6:52–58.
   Google Scholar
• Suryawanshi RK, Patil CD, Borase HP, et al. Studies on production and biological potential of prodigiosin by Serratia marcescens. Appl Biochem Biotechnol. 2014;173(5):1209–1221.
   PubMed Web of Science ®Google Scholar
• John Jimtha C, Jishma P, Sreelekha S, et al. Antifungal properties of prodigiosin producing rhizospheric Serratia sp. Rhizosphere. 2017;3:105–108.
   Web of Science ®Google Scholar
• Gutiérrez-Román MI, Holguín-Meléndez F, Dunn MF, et al. Antifungal activity of Serratia marcescens CFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerella fijiensis, causal agent of black Sigatoka in banana (Musa spp.). BioControl. 2015;60(4):565–572.
   Web of Science ®Google Scholar
• Someya N, Nakajima M, Hirayae K, et al. Synergistic antifungal activity of chitinolytic enzymes and prodigiosin produced by biocontrol bacterium, Serratia marcescens strain b2 against gray mold pathogen, botrytis cinerea. J Gen Plant Pathol. 2001;67(4):312–317.
   Google Scholar
• Zhang H, Peng Y, Zhang S, et al. Algicidal effects of prodigiosin on the harmful algae Phaeocystis globosa. Front Microbiol. 2016;7:602.
   PubMed Web of Science ®Google Scholar
• Herráez R, Quesada R, Dahdah N, et al. Tambjamines and prodiginines: biocidal activity against Trypanosoma cruzi. Pharmaceutics. 2021;13(5):705 .
   PubMed Web of Science ®Google Scholar
• Da Silva Melo P, Durán N, Haun M. Cytotoxicity of prodigiosin and benznidazole on V79 cells. Toxicol Lett. 2000;116(3):237–242.
   PubMed Web of Science ®Google Scholar
• Ehrenkaufer G, Li P, Stebbins EE, et al. Identification of anisomycin, prodigiosin and obatoclax as compounds with broad-spectrum anti-parasitic activity. PLoS Negl Trop Dis. 2020;14(3):e0008150.
   PubMed Web of Science ®Google Scholar
• Genes C, Baquero E, Echeverri F, et al. Mitochondrial dysfunction in Trypanosoma cruzi: the role of serratia marcescens prodigiosin in the alternative treatment of Chagas disease. Parasites Vectors. 2011;4(1):66.
   PubMedGoogle Scholar
• Rahul S, Chandrashekhar P, Hemant B, et al. In vitro antiparasitic activity of microbial pigments and their combination with phytosynthesized metal nanoparticles. Parasitol Int. 2015;64(5):353–356.
   PubMed Web of Science ®Google Scholar
• Balamuth W, Brent MM. Biological studies on Entamoeba histolytica. IV. direct action of the antibiotic, prodigiosin. Proc Soc Exp Biol Med. 1950;75:374–378.
   PubMed Web of Science ®Google Scholar
• Ehrenkaufer GM, Suresh S, Solow-Cordero D, et al. High-throughput screening of Entamoeba identifies compounds which target both life cycle stages and which are effective against metronidazole resistant parasites. Front Cell Infect Microbiol. 2018;8. 10.3389/fcimb.2018.00276
   PubMed Web of Science ®Google Scholar
• Davidson DE, Johnsen DO, Tanticharoenyos P, et al. Evaluating new antimalarial drugs against trophozoite induced Plasmodium cynomolgi malaria in rhesus monkeys. Am J Trop Med Hyg. 1976;25(1):26–33.
   PubMed Web of Science ®Google Scholar
• Isaka M, Jaturapat A, Kramyu J, et al. potent in vitro antimalarial activity of metacycloprodigiosin isolated from Streptomyces spectabilis BCC 4785. Antimicrob Agents Chemother. 2002;46(4):1112–1113.
   PubMed Web of Science ®Google Scholar
• Lazaro JEH, Nitcheu J, Predicala RZ, et al. Heptyl prodigiosin, a bacterial, metabolite, is antimalarial in vivo and non-mutagenic in vitro. J Nat Toxins. 2002;11(4):367–377.
   PubMedGoogle Scholar
• Papireddy K, Smilkstein M, Kelly JX, et al. Antimalarial activity of natural and synthetic prodiginines. J Med Chem. 2011;54(15):5296–5306.
   PubMed Web of Science ®Google Scholar
• Patil CD, Patil SV, Salunke BK, et al. Prodigiosin produced by Serratia marcescens NMCC46 as a mosquito larvicidal agent against Aedes aegypti and anopheles stephensi. Parasitol Res. 2011;109(4):1179–1187.
   PubMed Web of Science ®Google Scholar
• Suryawanshi RK, Patil CD, Borase HP, et al. Mosquito larvicidal and pupaecidal potential of prodigiosin from Serratia marcescens and understanding its mechanism of action. Pestic Biochem Physiol. 2015;123:49–55.
   PubMed Web of Science ®Google Scholar
• Pérez-Tomás R, Montaner B, Llagostera E, et al. The prodigiosins, proapoptotic drugs with anticancer properties. Biochem Pharmacol. 2003;66(8):1447–1452.
   PubMed Web of Science ®Google Scholar
• Soto-Cerrato V, Llagostera E, Montaner B, et al. Mitochondria-mediated apoptosis operating irrespective of multidrug resistance in breast cancer cells by the anticancer agent prodigiosin. Biochem Pharmacol. 2004;68(7):1345–1352.
   PubMed Web of Science ®Google Scholar
• Llagostera E, Soto-Cerrato V, Montaner B, et al. Prodigiosin induces apoptosis by acting on mitochondria in human lung cancer cells. Ann N Y Acad Sci. 2003;1010(1):178–181.
   PubMedGoogle Scholar
• Francisco R, Pérez-Tomás R, Gimènez-Bonafé P, et al. Mechanisms of prodigiosin cytotoxicity in human neuroblastoma cell lines. Eur J Pharmacol. 2007;572(2–3):111–119.
   PubMed Web of Science ®Google Scholar
• Park G, Tomlinson JT, Melvin MS, et al. Zinc and copper complexes of prodigiosin: implications for copper-mediated double-strand DNA cleavage. Org Lett. 2003;5(2):113–116.
   PubMed Web of Science ®Google Scholar
• Díaz-Ruiz C, Montaner B, Pérez-Tomás R. Prodigiosin induces cell death and morphological changes indicative of apoptosis in gastric cancer cell line HGT-1. Histol Histopathol. 2001;16(2):415–421.
   PubMed Web of Science ®Google Scholar
• Chiu W-J, Lin S-R, Chen Y-H, et al. Prodigiosin-emerged PI3K/Beclin-1-independent pathway elicits autophagic cell death in doxorubicin-sensitive and -resistant lung cancer. J Clin Med. 2018;7(10):321.
   PubMed Web of Science ®Google Scholar
• Wang Z, Li B, Zhou L, et al. Prodigiosin inhibits Wnt/β-catenin signaling and exerts anticancer activity in breast cancer cells. Proc Natl Acad Sci U S A. 2016;113(46):13150–13155.
   PubMed Web of Science ®Google Scholar
• Nguyen VB, Chen SP, Nguyen TH, et al. Novel efficient bioprocessing of marine chitins into active anticancer prodigiosin. Mar Drugs. 2020;18(1):15.
   Web of Science ®Google Scholar
• Li D, Liu J, Wang X, et al. Biological potential and mechanism of prodigiosin from Serratia marcescens subsp. lawsoniana in human choriocarcinoma and prostate cancer cell lines. Int J Mol Sci. 2018;19(11):3465.
   PubMed Web of Science ®Google Scholar
• Llagostera E, Soto-Cerrato V, Joshi R, et al. High cytotoxic sensitivity of the human small cell lung doxorubicin-resistant carcinoma (GLC4/ADR) cell line to prodigiosin through apoptosis activation. Anticancer Drugs. 2005;16(4):393–399.
   PubMed Web of Science ®Google Scholar
• Anwar MM, Shalaby M, Embaby AM, et al. Prodigiosin/PU-H71 as a novel potential combined therapy for triple negative breast cancer (TNBC): preclinical insights. Sci Rep. 2020;10(1):14706.
   PubMed Web of Science ®Google Scholar
• Goard CA, Schimmer AD. An evidence-based review of obatoclax mesylate in the treatment of hematological malignancies. Core Evid. 2013;8:15–26.
   PubMedGoogle Scholar
• Lim B, Greer Y, Lipkowitz S, et al. Novel apoptosis-inducing agents for the treatment of cancer, a new arsenal in the toolbox. Cancers (Basel). 2019;11(8):1087.
   PubMed Web of Science ®Google Scholar
• Schimmer AD, Raza A, Carter TH, et al. A multicenter phase l/ll study of obatoclax mesylate administered as a 3- Or 24-hour infusion in older patients with previously untreated acute myeloid leukemia. PLoS One. 2014;9(10):e108694.
   PubMed Web of Science ®Google Scholar
• Goy A, Hernandez-Ilzaliturri FJ, Kahl B, et al. A phase I/II study of the pan Bcl-2 inhibitor obatoclax mesylate plus bortezomib for relapsed or refractory mantle cell lymphoma. Leuk Lymphoma. 2014;55(12):2761–2768.
   PubMed Web of Science ®Google Scholar
• Chen S, Ren Y, Duan P. Biomimetic nanoparticle loading obatoclax mesylate for the treatment of non-small-cell lung cancer (NSCLC) through suppressing Bcl-2 signaling. Biomed Pharmacother. 2020;129:110371.
   PubMed Web of Science ®Google Scholar
• Islan GA, Cacicedo ML, Bosio VE, et al. Advances in smart nanopreparations for oral drug delivery. Sma Pharm Nano. 2016: 479–521
   Google Scholar
• Su JC, Chen KF, Chen WL, et al. Synthesis and biological activity of obatoclax derivatives as novel and potent SHP-1 agonists. Eur J Med Chem. 2012;56:127–133.
   PubMed Web of Science ®Google Scholar
• Zheng D, Chen S, Cai K, et al.Prodigiosin inhibits cholangiocarcinoma cell proliferation and induces apoptosis via suppressing SNAREs-dependent autophagy.Cancer Cell Int.2021;21(1):1–12.
   PubMed Web of Science ®Google Scholar
• Williamson NR, Fineran PC, Gristwood T, et al. Anticancer and immunosuppressive properties of bacterial prodiginines. Future Microbiol. 2007;2(6):605–618.
   PubMed Web of Science ®Google Scholar
• Davey NE, Travé G, Gibson TJ. How viruses hijack cell regulation. Trends Biochem Sci. 2011;36(3):159–169.
   PubMed Web of Science ®Google Scholar
• Suba K, Stalin A, Girija A, et al. Homology modeling and docking analysis of prodigiosin from Serratia marcescens. Biotechnology. 2013;55:12897–12902.
   Google Scholar
• Zhou W, Zeng C, Liu RH, et al. Antiviral activity and specific modes of action of bacterial prodigiosin against Bombyx mori nucleopolyhedrovirus in vitro. Appl Microbiol Biotechnol. 2016;100(9):3979–3988.
   PubMed Web of Science ®Google Scholar
• Suryawanshi RK, Koujah L, Patil CD, et al. Bacterial pigment prodigiosin demonstrates a unique antiherpesvirus activity that is mediated through inhibition of prosurvival signal transducers. J Virol. 2020;94(13). DOI:10.1128/JVI.00251-20
   Web of Science ®Google Scholar
• Varghese FS, Rausalu K, Hakanen M, et al. Obatoclax inhibits alphavirus membrane fusion by neutralizing the acidic environment of endocytic compartments. Antimicrob Agents Chemother. 2017;61(3). DOI:10.1128/AAC.02227-16
   PubMed Web of Science ®Google Scholar
• Varghese FS, van Woudenbergh E, Overheul GJ, et al. Berberine and obatoclax inhibit sars-cov-2 replication in primary human nasal epithelial cells in vitro. Viruses. 2021;13(2):282.
   PubMed Web of Science ®Google Scholar
• Mao B, Le-Trilling VTK, Wang K, et al. Obatoclax inhibits SARS-CoV-2 entry by altered endosomal acidification and impaired cathepsin and furin activity in vitro. Emerg Microbes Infect. 2022;11(1):483–497.
   PubMed Web of Science ®Google Scholar
• Patil CD, Suryawanshi RK, Koujah L, et al. Antiviral efficacy of prodigiosin against corneal herpes simplex virus infection. Invest Ophthalmol Vis Sci. 2020;61:2982.
   Web of Science ®Google Scholar
• Guryanov ID, Karamova NS, Yusupova DV, et al. Bacterial pigment prodigiosin and its genotoxic effect. Russ J Bioorganic Chem. 2013;39(1):106–111.
   Web of Science ®Google Scholar
• Han SB, Kim HM, Kim YH, et al. T-cell specific immunosuppression by prodigiosin isolated from Serratia marcescens. Int J Immunopharmacol. 1998;20(1–3):1–13.
   PubMed Web of Science ®Google Scholar
• Li X, Tan X, Chen Q, et al. Prodigiosin of Serratia marcescens zpg19 alters the gut microbiota composition of Kunming mice. Molecules. 2021;26(8):2156.
   PubMed Web of Science ®Google Scholar
• Desai N. Challenges in development of nanoparticle-based therapeutics. AAPS J. 2022 Apr 19;14(2):282–295.
   Google Scholar
• Dozie-Nwachukwu SO, Danyuo Y, Obayemi JD, et al. Extraction and encapsulation of prodigiosin in chitosan microspheres for targeted drug delivery. Mater Sci Eng C. 2017;71:268–278.
   PubMed Web of Science ®Google Scholar
• Obayemi JD, Danyuo Y, Dozie-Nwachukwu S, et al. PLGA-based microparticles loaded with bacterial- synthesized prodigiosin for anticancer drug release: effects of particle size on drug release kinetics and cell viability. Mater Sci Eng C. 2016;66:51–65.
   PubMed Web of Science ®Google Scholar
• El-Batal AI, El-Hendawy HH, Faraag AHI. In silicoandIn vitrocytotoxic effect of prodigiosin-conjugated silver nanoparticles on liver cancer cells (HepG2). Biotechnologia. 2017;98(3):225–243.
   Google Scholar
• Dozie-Nwachukwu SO, Obayemi JD, Danyuo Y, et al. A comparative study of the adhesion of biosynthesized gold and conjugated gold/prodigiosin nanoparticles to triple negative breast cancer cells. J Mater Sci Mater Med. 2017;28(9). DOI:10.1007/s10856-017-5943-2
   Web of Science ®Google Scholar
• Zhao K, Li D, Cheng G, et al. Targeted delivery prodigiosin to choriocarcinoma by peptide-guided dendrigraft poly-l-lysines nanoparticles. Int J Mol Sci. 2019;20(21):5458.
   PubMed Web of Science ®Google Scholar
• Arivizhivendhan KV, Mahesh M, Murali R, et al. Prodigiosin-iron-oxide-carbon matrix for efficient antibiotic-resistant bacterial disinfection of contaminated water. ACS Sustain Chem Eng. 2019;7(3):3164–3175.
   Web of Science ®Google Scholar
• Rastegari B, Karbalaei-Heidari HR, Zeinali S, et al. The enzyme-sensitive release of prodigiosin grafted β-cyclodextrin and chitosan magnetic nanoparticles as an anticancer drug delivery system: synthesis, characterization and cytotoxicity studies. Colloids Surf B Biointerfaces. 2017;158:589–601.
   PubMed Web of Science ®Google Scholar
• Ren Y, Gong J, Fu R, et al. Antibacterial dyeing of silk with prodigiosins suspension produced by liquid fermentation. J Clean Prod. 2018;201:648–656.
   Web of Science ®Google Scholar
• Namazkar S, Ahmad WA. Spray-dried prodigiosin from Serratia marcescens as a colorant. Biosci Biotechnol Res Asia. 2013;10(1):69–76.
   Google Scholar
• Guryanov I, Naumenko E, Akhatova F, et al. Selective cytotoxic activity of prodigiosin@halloysite nanoformulation. Front Bioeng Biotechnol. 2020;8. DOI:10.3389/fbioe.2020.00424
   PubMed Web of Science ®Google Scholar
• Mokhtarian F, Rastegari B, Zeinali S, et al. Theranostic effect of folic acid functionalized MIL-100(Fe) for delivery of prodigiosin and simultaneous tracking-combating breast cancer. J Nanomater. 2022;2022:1–16.
   Web of Science ®Google Scholar
• Danyuo Y, Dozie-Nwachukwu S, Obayemi JD, et al. Swelling of poly(N-isopropylacrylamide) P(NIPA)-based hydrogels with bacterial-synthesized prodigiosin for localized cancer drug delivery. Mater Sci Eng C. 2016;59:19–29.
   PubMed Web of Science ®Google Scholar
• Danyuo Y, Ani CJ, Salifu AA, et al. anomalous release kinetics of prodigiosin from poly-N-Isopropyl-acrylamide based hydrogels for the treatment of triple negative breast cancer. Sci Rep. 2019;9(1): DOI:10.1038/s41598-019-39578-4.
   PubMedGoogle Scholar
• Arivizhivendhan KV, Boopathy R, Maharaja P, et al. Bioactive prodigiosin-impregnated cellulose matrix for the removal of pathogenic bacteria from aqueous solution. RSC Adv. 2015;5(84):68621–68631.
   Web of Science ®Google Scholar
• Amorim LFA, Mouro C, Riool M, et al. Antimicrobial food packaging based on prodigiosin-Incorporated double-layered bacterial cellulose and chitosan composites. Polymers (Basel). 2022;14(2):315.
   PubMed Web of Science ®Google Scholar
• Akpan UM, Pellegrini M, Obayemi JD, et al. Prodigiosin-loaded electrospun nanofibers scaffold for localized treatment of triple negative breast cancer. Mater Sci Eng C. 2020;114:110976.
   PubMed Web of Science ®Google Scholar
• Danyuo Y, Obayemi JD, Dozie-Nwachukwu S, et al. Prodigiosin release from an implantable biomedical device: kinetics of localized cancer drug release. Mater Sci Eng C. 2014;42:734–745.
   PubMed Web of Science ®Google Scholar
• Danyuo Y, Ani CJ, Obayemi JD, et al. Prodigiosin release from an implantable biomedical device: effect on cell viability. Adv Mater Res. 2015;1132:3–18.
   Google Scholar
• Qian Z, Wang K, Liu S, et al. Quantitative prediction of paravalvular leak in transcatheter aortic valve replacement based on tissue-mimicking 3D printing. JACC Cardiovasc Imaging. 2017;10(7):719–731.
   PubMed Web of Science ®Google Scholar
• Wang K, Ho CC, Zhang C, et al. A review on the 3D printing of functional structures for medical phantoms and regenerated tissue and organ applications. Engineering. 2017;3(5):653–662.
   Web of Science ®Google Scholar
• Mathew E, Pitzanti G, Larrañeta E, et al. Three-dimensional printing of pharmaceuticals and drug delivery devices. Pharmaceutics. 2020;12(3):266.
   PubMed Web of Science ®Google Scholar
• Vithani K, Goyanes A, Jannin V, et al. An overview of 3D printing technologies for soft materials and potential opportunities for lipid-based drug delivery systems. Pharm Res. 2019;36(1). 10.1007/s11095-018-2531-1
   PubMed Web of Science ®Google Scholar
• Kobayashi N, Ichikawa Y. A protein associated with prodigiosin formation in Serratia marcescens. Microbiol Immunol. 1989;33(4):257–263.
   PubMed Web of Science ®Google Scholar
• Rastegari B, Karbalaei-Heidari HR, Yousefi R, et al. Interaction of prodigiosin with HSA and β-Lg: spectroscopic and molecular docking studies. Bioorg Med Chem. 2016;24(7):1504–1512.
   PubMed Web of Science ®Google Scholar
• Krishna PS, Vani K, Prasad MR, et al. In -silico molecular docking analysis of prodigiosin and cycloprodigiosin as COX-2 inhibitors. Springerplus. 2013;2(1). 10.1186/2193-1801-2-172
   PubMedGoogle Scholar
• Chang TM, Sinharay S, Astashkin AV, et al. Prodigiosin analogue designed for metal coordination: stable zinc and copper pyrrolyldipyrrins. Inorg Chem. 2014;53(14):7518–7526.
   PubMed Web of Science ®Google Scholar
• Mohammed SJ, Luti KJK. A kinetic model for prodigiosin production by Serratia marcescens as a bio-colorant in bioreactor. AIP Conf Proc. 2022 May 5; 2213:020027.
   Google Scholar
• Sumathi C, Mohanapriya D, Swarnalatha S, et al. Production of prodigiosin using tannery fleshing and evaluating its pharmacological effects. Sci World J. 2014;2014:1–8.
   Google Scholar
• Naik C, Srisevita JM, Shushma KN, et al. Peanut oil cake: a novel substrate for enhanced cell growth and prodigiosin production from Serratia marcescens CF-53. J Res Biol [Internet] 2012 cited 2022 May 5; 2:549–557. [Internet]. http://jresearchbiology.com/
   Google Scholar
• Song MJ, Bae J, Lee DS, et al. Purification and characterization of prodigiosin produced by integrated bioreactor from serratia sp. KH-95. J Biosci Bioeng [Internet] 2006 cited 2022 May 5; 101:157–161. Available from.;(2):. https://pubmed.ncbi.nlm.nih.gov/16569612/
   PubMed Web of Science ®Google Scholar
• Liu X, Wang Y, Sun S, et al. Mutant breeding of Serratia marcescens strain for enhancing prodigiosin production and application to textiles. Prep Biochem Biotechnol. May 5; 43(3):271–284.
   PubMed Web of Science ®Google Scholar
• A KV, Mahesh M, Boopathy R, et al. A novel method for the extraction of prodigiosin from bacterial fermenter integrated with sequential batch extraction reactor using magnetic iron oxide. Process Biochem. 2016;51(10):1731–1737.
   Web of Science ®Google Scholar
• Mahesh MAK, Sekaran GRMR. Bioactive prodigiosin isolated from Serratia marcescens using solid state fermenter and its bactericidal activity compared with conventional antibiotics. J Microb Biochem Technol. 2015;07(5):5.
   Google Scholar
• Dos Santos RA, Rodríguez DM, da Silva LAR, et al. Enhanced production of prodigiosin by Serratia marcescens UCP 1549 using agrosubstrates in solid-state fer-mentation. Arch Microbiol. 2022 May 5; 203:4091–4100.
   Web of Science ®Google Scholar
• Li D, Liu J, Wang X, et al. Biological potential and mechanism of prodigiosin from Serratia marcescens subsp. lawsoniana in human choriocarcinoma and prostate cancer cell lines. Int J Mol Sci. 2022 May 5;19(11):3465.
   Google Scholar
• Dasgupta Mandal D, Majumdar S, Dey S, et al., Utilization of low-cost fatty acid sources by bacterial isolate for improved production of valuable prodigiosin. Lect Notes Bioeng. 2021;5(1):21–27.
   Google Scholar
• Parani K, Saha BK. Optimization of prodigiosin production from a strain of Serratia marcescens SR 1 and screening for antifungal activity. J Biol Control 2022 May 5; 22:73–79.
   Google Scholar
• Arslan NP. Use of wool protein hydrolysate as nitrogen source in production of microbial pigments. J Food Process Preserv. [Internet] 2021 cited 2022 May 5; 45:e15660. Available from.;(7):. https://onlinelibrary.wiley.com/doi/full/10.1111/jfpp.15660
   Web of Science ®Google Scholar
• Giri AV, Anandkumar N, Muthukumaran G, et al. A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiol. 2022 May 5; 4(1):1–10.
   Google Scholar
• Shahitha S, Poornima K. Enhanced production of prodigiosin production in Serratia marcescens. J Appl Pharm Sci. 2012;2:138–140.
   Google Scholar
• Lin C, Jia X, Fang Y, et al. Enhanced production of prodigiosin by Serratia marcescens FZSF02 in the form of pigment pellets. Electron J Biotechnol. 2019;40:58–64.
   Web of Science ®Google Scholar
• Nguyen VB, Chen SP, Nguyen TH, et al. Novel efficient bioprocessing of marine chitins into active anticancer prodigiosin. Mar Drugs. 2022 May 5; 18.
   Web of Science ®Google Scholar
• Wang SL, Wang CY, Yen YH, et al. Enhanced production of insecticidal prodigiosin from Serratia marcescens TKU011 in media containing squid pen. Process Biochem. 2012;47(11):1684–1690.
   Web of Science ®Google Scholar
• Wei YH, Chen WC. Enhanced production of prodigiosin-like pigment from Serratia marcescens SMΔR by medium improvement and oil-supplementation strategies. J Biosci Bioeng. 2022 May 5; 99(6):616–622.
   Google Scholar
• Kurbanoglu EB, Ozdal M, Ozdal OG, et al. Enhanced production of prodigiosin by Serratia marcescens MO-1 using ram horn peptone. Brazilian J Microbiol. 2022 May 5; 46(2):631–637.
   Google Scholar
• Liu W, Yang J, Tian Y, et al. An in situ extractive fermentation strategy for enhancing prodigiosin production from Serratia marcescens bwl1001 and its application to inhibiting the growth of Microcystis aeruginosa. Biochem Eng J. 2021;166:107836.
   Web of Science ®Google Scholar
• Cang S, Sanada M, Johdo O, et al. High production of prodigiosin by Serratia marcescens grown on ethanol. Biotechnol Lett. 2022 May 5; 22(22):1761–1765.
   Google Scholar
• Xia S, Veony E, Yang Q. Kitchen waste as a novel available substrate for prodigiosin production by Serratia marcescens. IOP Conf Ser Earth Environ Sci. 2022 May 5; 171:012037.
   Google Scholar
• Picha P, Kale D, Dave I, et al. Comparative studies on prodigiosin production by Serratia marcescens using various crude fatty acid sources-its characterization and applications. IntJCurrMicrobiolAppSci. 2022 May 5; 2:254–267.
   Google Scholar
• Pan X, Sun C, Tang M, et al. Loss of serine-type D-Ala-D-Ala carboxypeptidase daca enhances prodigiosin production in Serratia marcescens. Front Bioeng Biotechnol. 2022 May 5;7.
   Google Scholar
• Suryawanshi RK, Patil CD, Borase HP, et al. Studies on production and biological potential of prodigiosin by Serratia marcescens. Appl Biochem Biotechnol. [Internet] 2014 cited 2022 May 5; 173:1209–1221. [Internet].;(5):. https://pubmed.ncbi.nlm.nih.gov/24781979/
   PubMed Web of Science ®Google Scholar
• Sun Y, Wang L, Pan X, et al. Improved Prodigiosin production by relieving CpxR temperature-sensitive inhibition. Front Bioeng Biotechnol [Internet] 2020 cited 2022 May 5; 8:344. Available from.
   PubMed Web of Science ®Google Scholar
• Chen WC, Tsai MJ, Soo PC, et al. Construction and co-cultivation of two mutant strains harboring key precursor genes to produce prodigiosin. J Biosci Bioeng. 2018;126(6):783–789.
   PubMed Web of Science ®Google Scholar
• De Araújo HWC, Fukushima K, Takaki GMC. Prodigiosin production by Serratia marcescens UCP 1549 using renewable-resources as a low cost substrate. Molecules. 2022 May 5; 15:6931–6940.
   Google Scholar
• Bhagwat A, Padalia U. Optimization of prodigiosin biosynthesis by Serratia marcescens using unconventional bioresources. J Genet Eng Biotechnol. 2022 May 5; 18(1).
   Web of Science ®Google Scholar
• Danevčič T, Borić Vezjak M, Zorec M, et al. Prodigiosin-A multifaceted Escherichia coli antimicrobial agent. PLoS One. 2016;11(9):e0162412.
   PubMed Web of Science ®Google Scholar
• Tomás RP, Ruir CD, Montaner B. Prodigiosin induces cell death and morphological changes indicative of apoptosis in gastric cancer cell line HGT-1. Histol Histopathol. 2001;16(2):415–421.
   PubMed Web of Science ®Google Scholar
• Yamamoto D, Kiyozuka Y, Uemura Y, et al. Cycloprodigiosin hydrochloride, a H+/Cl− symporter, induces apoptosis in human breast cancer cell lines. J Cancer Res Clin Oncol. 2000;126(4):191–197.
   PubMed Web of Science ®Google Scholar
• Montaner B, Pérez-Tomás R. Prodigiosin-induced apoptosis in human colon cancer cells. Life Sci. 2001;68(17):2025–2036.
   PubMed Web of Science ®Google Scholar
• Montaner B, Navarro S, Piqué M, et al. Prodigiosin from the supernatant of Serratia marcescens induces apoptosis in haematopoietic cancer cell lines. Br J Pharmacol. 2000;131(3):585–593.
   PubMed Web of Science ®Google Scholar
• Campas C, Dalmau M, Montaner B, et al. Prodigiosin induces apoptosis of B and T cells from B-cell chronic lymphocytic leukemia. Leukemia. 2003;17(4):746–750.
   PubMed Web of Science ®Google Scholar