Genetically distinct pestiviruses pave the way to improved classical swine fever marker vaccine candidates based on the chimeric pestivirus concept

References

• Postel A, Austermann-Busch S, Petrov A, et al. Epidemiology, diagnosis and control of classical swine fever: Recent developments and future challenges. Transbound Emerg Dis. 2017 Aug. doi:10.1111/tbed.12676.
   Web of Science ®Google Scholar
• Moennig V, Becher P. Pestivirus control programs: how far have we come and where are we going? Anim Health Res Rev. 2015 Jun;16(1):83–87.
   PubMed Web of Science ®Google Scholar
• Holinka LG, Fernandez-Sainz I, O'Donnell V, et al. Development of a live attenuated antigenic marker classical swine fever vaccine. Virology. 2009 Feb;384(1):106–113.
   PubMed Web of Science ®Google Scholar
• Holinka LG, Fernandez-Sainz I, Sanford B, et al. Development of an improved live attenuated antigenic marker CSF vaccine strain candidate with an increased genetic stability. Virology. 2014 Dec:471–473:13–18.
   PubMed Web of Science ®Google Scholar
• Holinka LG, O'Donnell V, Risatti GR, et al. Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G. PLoS One. 2017;12(5):e0177433.
   PubMed Web of Science ®Google Scholar
• Reimann I, Depner K, Trapp S, et al. An avirulent chimeric pestivirus with altered cell tropism protects pigs against lethal infection with classical swine fever virus. Virology. 2004 Apr;322(1):143–157.
   PubMed Web of Science ®Google Scholar
• Blome S, Wernike K, Reimann I, et al. A decade of research into classical swine fever marker vaccine CP7_E2alf (Suvaxyn® CSF marker): a review of vaccine properties. Vet Res. 2017 Sep;48(1):51.
   PubMed Web of Science ®Google Scholar
• Meyer D, Fritsche S, Luo Y, et al. The double-antigen ELISA concept for early detection of Erns -specific classical swine fever virus antibodies and application as an accompanying test for differentiation of infected from marker vaccinated animals. Transbound Emerg Dis. 2017 Feb:2013–2022.
   PubMed Web of Science ®Google Scholar
• Meyer D, Loeffen W, Postel A, et al. Reduced specificity of Erns antibody ELISAs for samples from piglets with maternally derived antibodies induced by vaccination of sows with classical swine fever marker vaccine CP7_E2alf. Transbound Emerg Dis. 2018 Apr;65(2):e505–e5e8.
   PubMed Web of Science ®Google Scholar
• Xia H, Harimoorthy R, Vijayaraghavan B, et al. Differentiation of classical swine fever virus infection from CP7_E2alf marker vaccination by a multiplex microsphere immunoassay. Clin Vaccine Immunol. 2015 Jan;22(1):65–71.
   PubMedGoogle Scholar
• Lim SI, Choe S, Kim KS, et al. Assessment of the efficacy of an attenuated live marker classical swine fever vaccine (Flc-LOM-BE(rns)) in pregnant sows. Vaccine. 2019 Jun;37(27):3598–3604.
   PubMed Web of Science ®Google Scholar
• Choe S, Cha RM, Yu D-S, et al. Rapid spread of classical swine fever virus among South Korean wild boars in areas near the border with North Korea. Pathogens. 2020 Mar;9(4):244.
   PubMed Web of Science ®Google Scholar
• Gong W, Li J, Wang Z, et al. Commercial E2 subunit vaccine provides full protection to pigs against lethal challenge with 4 strains of classical swine fever virus genotype 2. Vet Microbiol. 2019 Oct;237:108403.
   PubMed Web of Science ®Google Scholar
• Meyers G, Thiel HJ, Rumenapf T. Classical swine fever virus: recovery of infectious viruses from cDNA constructs and generation of recombinant cytopathogenic defective interfering particles. J Virol. 1996 Mar;70(3):1588–1595.
   PubMed Web of Science ®Google Scholar
• Gallei A, Rumenapf T, Thiel H-J, et al. Characterization of helper virus-independent cytopathogenic classical swine fever virus generated by an in vivo RNA recombination system. J Virol. 2005 Feb;79(4):2440–2448.
   PubMed Web of Science ®Google Scholar
• Meyer D, Schmeiser S, Postel A, et al. Transfection of RNA from organ samples of infected animals represents a highly sensitive method for virus detection and recovery of classical swine fever virus. PLoS One. 2015;10(5):e0126806.
   PubMed Web of Science ®Google Scholar
• Peters W, Greiser-Wilke I, Moennig V, et al. Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. Vet Microbiol. 1986 Sep;12(3):195–200.
   PubMed Web of Science ®Google Scholar
• Becher P, Schmeiser S, Oguzoglu TC, et al. Complete genome sequence of a novel pestivirus from sheep. J Virol. 2012 Oct;86(20):11412.
   PubMed Web of Science ®Google Scholar
• Anonymous. EU diagnostic manual for classical swine fever (CSF) diagnosis: Technical Part (Fourth Draft March 2020). EU and OIE Reference Laboratory for CSF, Homepage; 2020 [cited 2020 Jul 1]. Available from: https://www.tiho-hannover.de/fileadmin/user_upload/tiho_hannover/kliniken_institute/21_virologie/eurl/_Technical_Annex_Draft_2020.pdf.
   Google Scholar
• Mittelholzer C, Moser C, Tratschin J-D, et al. Analysis of classical swine fever virus replication kinetics allows differentiation of highly virulent from avirulent strains. Vet Microbiol. 2000 Jun;74(4):293–308.
   PubMed Web of Science ®Google Scholar
• Hoffmann B, Beer M, Schelp C, et al. Validation of a real-time RT-PCR assay for sensitive and specific detection of classical swine fever. J Virol Methods. 2005 Dec;130(1-2):36–44.
   PubMed Web of Science ®Google Scholar
• Koenig P, Lange E, Reimann I, et al. CP7_E2alf: a safe and efficient marker vaccine strain for oral immunisation of wild boar against classical swine fever virus (CSFV). Vaccine. 2007 Apr;25(17):3391–3399.
   PubMed Web of Science ®Google Scholar
• Renson P, Le Dimna M, Keranflech A, et al. CP7_E2alf oral vaccination confers partial protection against early classical swine fever virus challenge and interferes with pathogeny-related cytokine responses. Vet Res. 2013 Feb;44(9).
   PubMedGoogle Scholar
• Pannhorst K, Frohlich A, Staubach C, et al. Evaluation of an Erns-based enzyme-linked immunosorbent assay to distinguish classical swine fever virus–infected pigs from pigs vaccinated with CP7_E2alf. J Vet Diagn Invest. 2015 Jul;27(4):449–460.
   PubMed Web of Science ®Google Scholar
• Schroeder S, von Rosen T, Blome S, et al. Evaluation of classical swine fever virus antibody detection assays with an emphasis on the differentiation of infected from vaccinated animals. Rev Sci Tech. 2012 Dec;31(3):997–1010.
   PubMed Web of Science ®Google Scholar
• Blome S, Moss C, Reimann I, et al. Classical swine fever vaccines-state-of-the-art. Vet Microbiol. 2017 July;206:10–20.
   PubMed Web of Science ®Google Scholar
• Luo Y, Yuan Y, Ankenbauer RG, et al. Construction of chimeric bovine viral diarrhea viruses containing glycoprotein E rns of heterologous pestiviruses and evaluation of the chimeras as potential marker vaccines against BVDV. Vaccine. 2012 Jun;30(26):3843–3848.
   PubMed Web of Science ®Google Scholar
• Hulst MM, van Gennip HGP, Moormann RJM. Passage of classical swine fever virus in cultured swine kidney cells Selects virus variants that bind to heparan sulfate due to a single amino acid change in Envelope protein Erns. J Virol. 2000 Oct;74(20):9553–9561.
   PubMed Web of Science ®Google Scholar
• Koenig P, Hoffmann B, Depner KR, et al. Detection of classical swine fever vaccine virus in blood and tissue samples of pigs vaccinated either with a conventional C-strain vaccine or a modified live marker vaccine. Vet Microbiol. 2007 Mar;120(3-4):343–351.
   PubMed Web of Science ®Google Scholar
• Eble PL, Geurts Y, Quak S, et al. Efficacy of chimeric pestivirus vaccine candidates against classical swine fever: protection and DIVA characteristics. Vet Microbiol. 2013 Mar;162(2-4):437–446.
   PubMed Web of Science ®Google Scholar