Three neutralizing mAbs induced by MPXV A29L protein recognizing different epitopes act synergistically against orthopoxvirus

References

• Petersen E, Kantele A, Koopmans M, et al. Human monkeypox: epidemiologic and Clinical characteristics, diagnosis, and prevention. Infect Dis Clin North Am. 2019;33(4):1027–1043.
   PubMed Web of Science ®Google Scholar
• Krupovic M, Cvirkaite-Krupovic V, Bamford DH. Protein A33 responsible for antibody-resistant spread of vaccinia virus is homologous to C-type lectin-like proteins. Virus Res 2010;151(1):97–101.
   PubMed Web of Science ®Google Scholar
• Reynolds MG, McCollum AM, Nguete B, et al. Improving the care and treatment of monkeypox patients in low-resource settings: applying evidence from contemporary biomedical and smallpox biodefense research. Viruses. 2017;9(12).
   PubMed Web of Science ®Google Scholar
• Lum F-M, Torres-Ruesta A, Tay MZ, et al. Monkeypox: disease epidemiology, host immunity and clinical interventions. Nat Rev Immunol. 2022;22(10):597–613.
   PubMed Web of Science ®Google Scholar
• Bunge EM, Hoet B, Chen L, et al. The changing epidemiology of human monkeypox-A potential threat? a systematic review. PLoS Negl Trop Dis. 2022;16(2):e0010141.
   PubMed Web of Science ®Google Scholar
• Kirtane AR, Verma M, Karandikar P, et al. Nanotechnology approaches for global infectious diseases. Nat Nanotechnol. 2021;16(4):369–384.
   PubMed Web of Science ®Google Scholar
• Zhai L, Zhang L, Jiang Y, et al. Broadly neutralizing antibodies recognizing different antigenic epitopes act synergistically against the influenza B virus. J Med Virol 2023;95(1):e28106.
   PubMed Web of Science ®Google Scholar
• Wu Y, Wang F, Shen C, et al. A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2. Science. 2020;368(6496):1274–1278.
   PubMed Web of Science ®Google Scholar
• Shen C, Chen J, Li R, et al. A multimechanistic antibody targeting the receptor binding site potently cross-protects against influenza B viruses. Sci Transl Med. 2017;9(412):eaam5752.
   PubMed Web of Science ®Google Scholar
• Berhanu A, Wilson Rebecca L, Kirkwood-Watts Dana L, et al. Vaccination of BALB/c mice with escherichia coli-expressed vaccinia virus proteins A27L, B5R, and D8L protects mice from lethal vaccinia virus challenge. J Virol. 2008;82(7):3517–3529.
   PubMed Web of Science ®Google Scholar
• Gilchuk I, Gilchuk P, Sapparapu G, et al. Cross-neutralizing and protective human antibody specificities to poxvirus infections. Cell. 2016;167(3):684–94.e9.
   PubMed Web of Science ®Google Scholar
• Benhnia Mohammed R-E-I, McCausland Megan M, Moyron J, et al. Vaccinia virus extracellular enveloped virion neutralization In vitro and protection In vivo depend on complement. J Virol 2009;83(3):1201–1215.
   PubMed Web of Science ®Google Scholar
• Chen Z, Earl P, Americo J, et al. Chimpanzee/human mAbs to vaccinia virus B5 protein neutralize vaccinia and smallpox viruses and protect mice against vaccinia virus. Proc Natl Acad Sci USA. 2006;103(6):1882–1887.
   PubMed Web of Science ®Google Scholar
• Matho MH, Schlossman A, Meng X, et al. Structural and functional characterization of anti-A33 antibodies reveal a potent cross-species orthopoxviruses neutralizer. PLoS Pathog 2015;11(9):e1005148.
   PubMed Web of Science ®Google Scholar
• Chen Z, Earl P, Americo J, et al. Characterization of chimpanzee/human monoclonal antibodies to vaccinia virus A33 glycoprotein and Its variola virus homolog In vitro and in a vaccinia virus mouse protection model. J Virol 2007;81(17):8989–8995.
   PubMed Web of Science ®Google Scholar
• Gu X, Zhang Y, Jiang W, et al. Protective human anti-poxvirus monoclonal antibodies are generated from rare memory B cells isolated by multicolor antigen tetramers. Vaccines (Basel). 2022;10(7).
   Web of Science ®Google Scholar
• Gong SC, Lai CF, Esteban M. Vaccinia virus induces cell fusion at acid pH and this activity is mediated by the N-terminus of the 14-kDa virus envelope protein. Virology. 1990;178(1):81–91.
   PubMed Web of Science ®Google Scholar
• Chang TH, Chang SJ, Hsieh FL, et al. Crystal structure of vaccinia viral A27 protein reveals a novel structure critical for its function and complex formation with A26 protein. PLoS Pathog 2013;9(8):e1003563.
   PubMed Web of Science ®Google Scholar
• Ward BM, Weisberg AS, Moss B. Mapping and functional analysis of interaction sites within the cytoplasmic domains of the vaccinia virus A33R and A36R envelope proteins. J Virol 2003;77(7):4113–4126.
   PubMed Web of Science ®Google Scholar
• Law M, Hollinshead R, Smith GL. Antibody-sensitive and antibody-resistant cell-to-cell spread by vaccinia virus: role of the A33R protein in antibody-resistant spread. J Gen Virol 2002;83(Pt 1):209–222.
   PubMedGoogle Scholar
• Li M, Wang Y, Li C, et al. Development of monoclonal antibody-based antigens detection assays for orthopoxvirus and monkeypox virus. J Infect. 2022;85(6):702–769.
   Web of Science ®Google Scholar
• Li GM, Chiu C, Wrammert J, et al. Pandemic H1N1 influenza vaccine induces a recall response in humans that favors broadly cross-reactive memory B cells. Proc Natl Acad Sci USA 2012;109(23):9047–9052.
   PubMed Web of Science ®Google Scholar
• Mirdita M, Schütze K, Moriwaki Y, et al. ColabFold: making protein folding accessible to all. Nat Methods. 2022;19(6):679–682.
   PubMed Web of Science ®Google Scholar
• Brennan A, Wing Ki W, Fergus B, et al. Immunebuilder: deep-learning models for predicting the structures of immune proteins. bioRxiv. 2022:2022.11.04.514231.
   Google Scholar
• Pierce BG, Wiehe K, Hwang H, et al. ZDOCK server: interactive docking prediction of protein-protein complexes and symmetric multimers. Bioinformatics. 2014;30(12):1771–1773.
   PubMed Web of Science ®Google Scholar
• Desta IT, Porter KA, Xia B, et al. Performance and its limits in rigid body protein-protein docking. Structure. 2020;28(9):1071–81.e3.
   PubMed Web of Science ®Google Scholar
• Vajda S, Yueh C, Beglov D, et al. New additions to the ClusPro server motivated by CAPRI. Proteins. 2017;85(3):435–444.
   PubMed Web of Science ®Google Scholar
• Kozakov D, Hall DR, Xia B, et al. The ClusPro web server for protein–protein docking. Nat Protoc. 2017;12(2):255–278.
   PubMed Web of Science ®Google Scholar
• Kozakov D, Beglov D, Bohnuud T, et al. How good is automated protein docking? Proteins. 2013;81(12):2159–2166.
   PubMed Web of Science ®Google Scholar
• DeLano WL. The PyMOL molecular graphic system (Delano Scientific, SanCarlos, CA). 1.7.0.1. 2002.
   Google Scholar
• Ku Z, Xie X, Davidson E, et al. Molecular determinants and mechanism for antibody cocktail preventing SARS-CoV-2 escape. Nat Commun. 2021;12(1):469.
   PubMed Web of Science ®Google Scholar
• Weinreich DM, Sivapalasingam S, Norton T, et al. REGN-COV2, a neutralizing antibody cocktail, in outpatients with COVID-19. N Engl J Med 2021;384(3):238–251.
   PubMed Web of Science ®Google Scholar
• Gilchuk P, Murin CD, Milligan JC, et al. Analysis of a therapeutic antibody cocktail reveals determinants for cooperative and broad ebolavirus neutralization. Immunity. 2020;52(2):388–403.e12.
   PubMed Web of Science ®Google Scholar
• Monticelli SR, Earley AK, Stone R, et al. Vaccinia virus glycoproteins A33, A34, and B5 form a complex for efficient endoplasmic reticulum to trans-Golgi network transport. J Virol 2020;94(7).
   Web of Science ®Google Scholar
• Chen Z, Earl P, Americo J, et al. Characterization of chimpanzee/human monoclonal antibodies to vaccinia virus A33 glycoprotein and its variola virus homolog in vitro and in a vaccinia virus mouse protection model. J Virol 2007;81(17):8989–8995.
   PubMed Web of Science ®Google Scholar
• Paran N, Lustig S. Complement-bound human antibodies to vaccinia virus B5 antigen protect mice from virus challenge. Expert Rev Vaccines. 2010;9(3):255–259.
   PubMed Web of Science ®Google Scholar
• Bisht H, Weisberg AS, Moss B. Vaccinia virus l1 protein is required for cell entry and membrane fusion. J Virol 2008;82(17):8687–8694.
   PubMed Web of Science ®Google Scholar
• Alkhalil A, Strand S, Mucker E, et al. Inhibition of monkeypox virus replication by RNA interference. Virol J. 2009;6:188.
   PubMed Web of Science ®Google Scholar