Determination of apomixis by flow cytometry in two species of Lachemilla (Rosaceae) in Ecuador

References

• Gaviria J, Sinópsis del género Lachemilla (Focke) Rydberg (Rosaceae) para Venezuela. [Synopsis of the genus Lachemilla (Focke) Rydberg (Rosaceae) for Venezuela]. Plantula [Internet]. 1996 [cited 2013 Nov 2];1(3):189–212. Available from: http://www.paramo.org/files/recursos/sinopsis_Lachemilla.pdf
   Google Scholar
• Romoleroux K, The genus Lachemilla (Rosaceae) in the northern Andes of South America. Lyonia [Internet]. 2004 [cited 2013 Nov 2];7(1):21–32. Available from: http://www.lyonia.org/articles/rbussmann/article_232/pdf/article.pdf
   Google Scholar
• Sklenář P, Luteyn JL, Ulloa Ulloa C, et al. Flora genérica de los Páramos: guía ilustrada de las plantas vasculares. [Generic flora of the Páramos: illustrated guide to vascular plants]. The New York Botanical Garden Press; 2005.
   Google Scholar
• Gehrke B, Bräuchler C, Romoleroux K, et al., Molecular phylogenetics of Alchemilla, Aphanes and Lachemilla (Rosaceae) inferred from plastid and nuclear intron and spacer DNA sequences, with comments on generic classification. Mol Phylogenet Evol [Internet]. 2008 [cited 2013 Nov 2];47(3):1030–1044. Available from: http://www.sciencedirect.com/science/article/pii/S1055790308001000
   PubMed Web of Science ®Google Scholar
• Romoleroux K. Rosaceae 79. In: Harling G, Andersson L, editors. Flora of Ecuador vol 56. Stockholm: Publishing House of the Swedish Research Council; 1996. p. 89–132.
   Google Scholar
• Sklenář P, Jørgensen PM. Distribution patterns of páramo plants in Ecuador. J Biogeogr [Internet]. 1999;26(4):681–691.
   Web of Science ®Google Scholar
• Luteyn JL. Páramos: a checklist of plant diversity, geographical distribution, and botanical literature. New York: New York Botanical Garden Press; 1999. p. 124–125.
   Google Scholar
• Morales-Briones DF Relaciones filogenéticas de Lachemilla (Focke) Rydb. (Rosaceae) inferidas a partir de secuencias de ADN nuclear y cloroplástico, con énfasis en las especies del norte de Sudamérica. [Phylogenetic relationships of Lachemilla (Focke) Rydb. (Rosaceae) inferred from nuclear and chloroplastic DNA sequences, with emphasis on northern species of South America] [Thesis] Pontificia Universidad Católica del Ecuador; 2009.
   Google Scholar
• Romoleroux K, Morales-Briones DF. Lachemilla jaramilloi and L. talamanquensis spp. nov. (Rosaceae) from Ecuador and Costa Rica. Nord J Bot [Internet]. Blackwell Publishing Ltd. 2012;30(6):732–736.
   Web of Science ®Google Scholar
• Judd WS, Campbell CS, Kellogg EA, et al. Plant Systematics: a phylogenetic approach. Third ed. Sunderland (MA):  Sinauer Associates, Inc.; 2008.
   Google Scholar
• Campbell CS, Greene CW, Dickinson TA. Reproductive biology in Subfam. Maloideae (Rosaceae). Syst Bot [Internet]. American Society of Plant Taxonomists. 1991;16(2):333–349. Available from: http://www.jstor.org/stable/2419284
   Web of Science ®Google Scholar
• Bolmgren K, Eriksson O, Linder HP. Contrasting flowering phenology and species richness in abiotically and biotically pollinated angiosperms. Evolution (N Y) [Internet]. 2003;57(9):2001–2011.
   PubMed Web of Science ®Google Scholar
• Brown AO, McNeil JN, Pollination ecology of the high latitude, dioecious cloudberry (Rubus chamaemorus; Rosaceae). Am J Bot [Internet]. 2009 [cited 2013 Nov 2];96(6):1096–1107. Available from: http://www.amjbot.org/content/96/6/1096.short
   PubMed Web of Science ®Google Scholar
• Domic AI, Mamani E, Camilo G, Fenología reproductiva de la kewiña (Polylepis tomentella, Rosaceae) en la puna semihúmeda de Chuquisaca (Bolivia). [Reproductive phenology of the Kewiña (Polylepis tomentella, Rosaceae) in the semi-humid puna of Chuquisaca (Bolivia)]. Ecol En Boliv [Internet]. 2013 [cited 2013 Nov 2];48(1):31–45. Available from: http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=&lng=es&nrm=iso&tlng=
   Google Scholar
• Pont AC. Observations on anthophilous Muscidae and other Diptera (Insecta) in Abisko National Park, Sweden. J Nat Hist [Internet]. Taylor & Francis. 1993 [cited 2013 Nov 1];27(3):631–643.
   Web of Science ®Google Scholar
• Matzk F, Meister A, Schubert I. An efficient screen for reproductive pathways using mature seeds of monocots and dicots. Plant J [Internet]. Blackwell Science Ltd. 2000;21(1):97–108.
   Google Scholar
• Bicknell RA, Koltunow AM, Understanding apomixis: recent advances and remaining conundrums. Plant Cell [Internet]. 2004 [cited 2013 Nov 1];16(Suppl(suppl_1)):S228–45. Available from: http://www.plantcell.org/content/16/suppl_1/S228
   PubMedGoogle Scholar
• Richards AJ, Apomixis in flowering plants: an overview. Philos Trans R Soc Lond B Biol Sci [Internet]. 2003 [cited 2013 Nov 2];358(1434):1085–1093. Available from: http://rstb.royalsocietypublishing.org/content/358/1434/1085.short
   PubMed Web of Science ®Google Scholar
• Matzk F. Reproduction mode screening. Flow Cytometry with Plant Cells [Internet]. Wiley-VCH Verlag GmbH & Co. KGaA. 2007;131–152. DOI:10.1002/9783527610921.ch6
   Google Scholar
• Pihu S, Hõimra J, Köster E, et al. Environmentally dependent morphological variability in seven apomictic microspecies from Alchemilla L. (Rosaceae). Folia Geobot [Internet]. 2009 [cited 2013 Nov 2];44(2):159–176.
   Web of Science ®Google Scholar
• Carneiro V, Dusi D, Ortiz J. Apomixis: occurrence, applications and improvements. Floric Ornam Plant Biotechnol [Internet]. 2006 [cited 2013 Nov 2];564–571. Available from: http://www17.plala.or.jp/gsbjapan/images/Vol1_sample.pdf
   Google Scholar
• Czapik R. Problems of apomictic reproduction in the families Compositae and Rosaceae. Folia Geobot [Internet]. 1996;31(3):381–387.
   Web of Science ®Google Scholar
• Heenan PB, Molloy BPJ, Bicknell RA, et al. Levels of apomictic and amphimictic seed formation in a natural population of Coprosma robusta (Rubiaceae) in Riccarton Bush, Christchurch, New Zealand. New Zeal J Bot [Internet]. Taylor & Francis. 2003;41(2):287–291.
   Web of Science ®Google Scholar
• Hörandl E, Dobeš C, Suda J, et al. Apomixis is not prevalent in subnival to nival plants of the European Alps. Ann Bot [Internet]. 2011 [cited 2013 Nov 2]; 108(2):381–390. Available from: http://aob.oxfordjournals.org/content/108/2/381.short
   PubMed Web of Science ®Google Scholar
• Hörandl E, Cosendai A-C, Temsch EM. Understanding the geographic distributions of apomictic plants: a case for a pluralistic approach. Plant Ecol Divers [Internet]. Taylor & Francis. 2008 [cited 2013 Oct 30];1(2):309–320.
   PubMed Web of Science ®Google Scholar
• Arroyo MTK, Primack R, Armesto J. Community studies in pollination ecology in the high temperate Andes of Central Chile. I. Pollination mechanisms and altitudinal variation. Am J Bot [Internet]. Botanical Society of America. 1982;69(1):82–97. Available from: http://www.jstor.org/stable/2442833
   Web of Science ®Google Scholar
• Pendleton BK, Pendleton RL. Pollination Biology of Coleogyne ramosissima (Rosaceae). Southwest Nat [Internet]. Southwestern Association of Naturalists. 1998;43(3):376–380. Available from: http://www.jstor.org/stable/30055383
   Web of Science ®Google Scholar
• Sakai S, Thrips pollination of androdioecious Castilla elastica (Moraceae) in a seasonal tropical forest. Am J Bot [Internet]. 2001 [cited 2013 Nov 2];88(9):1527–1534. Available from: http://www.amjbot.org/content/88/9/1527.short
   PubMed Web of Science ®Google Scholar
• Pías B, Guitián P, Breeding system and pollen limitation in the masting tree Sorbus aucuparia L. (Rosaceae) in the NW Iberian Peninsula. Acta Oecologica [Internet]. 2006 [cited 2013 Nov 2];29(1):97–103. Available from: http://www.sciencedirect.com/science/article/pii/S1146609X05000949
   Web of Science ®Google Scholar
• Fagua JC, Gonzalez VH. Growth rates, reproductive phenology, and pollination ecology of Espeletia grandiflora (Asteraceae), a giant andean caulescent rosette. Plant Biol [Internet]. Blackwell Publishing Ltd. 2007;9(1):127–135.
   PubMed Web of Science ®Google Scholar
• Herrera J. Pollination relationships in southern spanish mediterranean shrublands. J Ecol [Internet]. British Ecological Society. 1988;76(1):274–287. Available from: http://www.jstor.org/stable/2260469
   Google Scholar
• Blanco MA, Barboza G, Pseudocopulatory pollination in Lepanthes (Orchidaceae: pleurothallidinae) by fungus gnats. Ann Bot [Internet]. 2005 [cited 2013 Nov 2];95(5):763–772. Available from: http://aob.oxfordjournals.org/content/95/5/763.short
   PubMed Web of Science ®Google Scholar
• Triplehorn CA, Johnson NF. Borror and Delong’s introduction to the study of insects. Seventh ed. Belmont (CA): Thomson Brooks/ Cole; 2005.
   Google Scholar
• Hanson PE, Gauld ID. Hymenoptera de la región neotropical. [Hymenoptera of the neotropical region]. Vol. 77. Madison, WI: Memoirs of the American Entomological Institute; 2006.
   Google Scholar
• Etcheverry AV, Alemán MM, Fleming TF, Flower morphology, pollination biology and mating system of the complex flower of Vigna caracalla (Fabaceae: papilionoideae). Ann Bot [Internet]. 2008 [cited 2013 Nov 2];102(3):305–316. Available from: http://aob.oxfordjournals.org/content/102/3/305.short
   PubMed Web of Science ®Google Scholar
• Ellis AG, Johnson SD, The evolution of floral variation without pollinator shifts in Gorteria diffusa (Asteraceae). Am J Bot [Internet]. 2009 [cited 2013 Oct 31];96(4):793–801. Available from: http://www.amjbot.org/content/96/4/793.short
   PubMed Web of Science ®Google Scholar
• Ollerton J, Masinde S, Meve U, et al., Fly pollination in Ceropegia (Apocynaceae: asclepiadoideae): biogeographic and phylogenetic perspectives. Ann Bot [Internet]. 2009 [cited 2013 Nov 2];103(9):1501–1514. Available from: http://aob.oxfordjournals.org/content/103/9/1501.short
   PubMed Web of Science ®Google Scholar
• Wodehouse RP. Preparation of pollen for microscopic examination. Bull Torrey Bot Club [Internet]. Torrey Botanical Society. 1933;60(6):417–421. Available from: http://www.jstor.org/stable/2480494
   Google Scholar
• Doležell J, Greilhuber J, Suda J. Estimation of nuclear DNA content in plants using flow cytometry. Nat Protoc [Internet]. 2007;2(9):2233–2244. Available from: http://www.ncbi.nlm.nih.gov/pubmed/17853881
   PubMed Web of Science ®Google Scholar
• Otto F. DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. Methods Cell Biol. 1990;33:105–110.
   PubMedGoogle Scholar
• Doležell J, Greilhuber J, Meister A, et al. Plant genome size estimation by flow cytometry: inter-laboratory comparison. Ann Bot. 1998;82:17–26.
   Web of Science ®Google Scholar
• Schranz ME, Dobes C, Koch MA, et al., Sexual reproduction, hybridization, apomixis, and polyploidization in the genus Boechera (Brassicaceae). Am J Bot [Internet]. 2005 [cited 2013 Nov 4];92(11):1797–1810. Available from: http://www.amjbot.org/content/92/11/1797.short
   PubMed Web of Science ®Google Scholar
• Dobeš C, Lückl A, Hülber K, et al., Prospects and limits of the flow cytometric seed screen-insights from Potentilla sensu lato (Potentilleae, Rosaceae). New Phytol [Internet]. 2013 [cited 2013 Nov 1];198(2):605–616. Available from: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=3618378&tool=pmcentrez&rendertype=abstract
   PubMed Web of Science ®Google Scholar
• Loureiro J, Suda J, Doležel J, et al. FLOWER: A Plant DNA Flow Cytometry Database. In: Flow Cytometry with Plant Cells [Internet]. Wiley-VCH Verlag GmbH & Co. KGaA; 2007. p. 423–438. DOI:10.1002/9783527610921.ch18
   Google Scholar
• Grey J, Kelly A, Ward S, et al. Seasonal changes in the stable isotope values of lake-dwelling chironomid larvae in relation to feeding and life cycle variability. Freshw Biol [Internet]. Blackwell Science Ltd. 2004;49(6):681–689.
   Web of Science ®Google Scholar
• Stephenson AG. Flower and fruit abortion: proximate causes and ultimate functions. Annu Rev Ecol Syst [Internet]. Annual Reviews. 1981;12:253–279. Available from: http://www.jstor.org/stable/2097112
   Web of Science ®Google Scholar
• Smithers P, Ramsay PM, Bond AN, et al. Macro-arthropod communities of the giant rosette plant, Espeletia pycnophylla subsp. angelensis. In: Pm R, editor. The ecology of Volcán Chiles: high-altitude ecosystems on the Ecuador-Colombia border. Plymouth: Pebble & Shell; 2001. p. 169–175.
   Google Scholar
• Kevan PG, Baker HG. Insects as flower visitors and pollinators. Annu Rev Entomol [Internet]. Annual Reviews. 1983;28(1):407–453.
   Google Scholar
• Larson BMH, Kevan PG, Inouye DW. Flies and flowers: taxonomic diversity of anthophiles and pollinators. Can Entomol. Cambridge Journals Online. 2001;133(04):439–465.
   Google Scholar
• Campbell J, Hanula JL. Efficiency of malaise traps and colored pan traps for collecting flower visiting insects from three forested ecosystems. J Insect Conserv [Internet]. Springer Netherlands. 2007;11(4):399–408.
   Web of Science ®Google Scholar
• Ssymank A, Ca K, Pape T, et al. Pollinating Flies (Diptera): A major contribution to plant diversity and agricultural production. Biodiversity [Internet]. Taylor & Francis. 2008;9(1–2):86–89.
   Google Scholar
• Dupont YL, Hansen DM, Olesen JM. Structure of a plant–flower-visitor network in the high-altitude sub-alpine desert of Tenerife, Canary Islands. Ecography (Cop) [Internet]. Munksgaard International Publishers. 2003;26(3):301–310.
   Web of Science ®Google Scholar
• Sakai S. Aristolochia spp. (Aristolochiaceae) pollinated by flies breeding on decomposing flowers in Panama. Am J Bot [Internet]. 2002;89(3):527–534. Available from: http://www.amjbot.org/content/89/3/527.abstract
   PubMed Web of Science ®Google Scholar
• Vinkenoog R, Scott R. Autonomous endosperm development in flowering plants: how to overcome the imprinting problem? Sex Plant Reprod [Internet]. Springer-Verlag. 2001;14(4):189–194.
   PubMedGoogle Scholar
• Morales-Briones DF, Romoleroux K, Kollar F, et al. Phylogeny and evolution of the neotropical radiation of Lachemilla (Rosaceae): uncovering a history of reticulate evolution and implications for infrageneric classification. Systematic Botany. 2018;43(1):17–34.
   Web of Science ®Google Scholar
• Dickinson TA, Lo E, Talent N. Polyploidy, reproductive biology, and Rosaceae: understanding evolution and making classifications. Plant Syst Evol [Internet]. 2007;266(1–2):59–78.
   Web of Science ®Google Scholar
• Sharbel TF, Mitchell-Olds T. Recurrent polyploid origins and chloroplast phylogeography in the Arabis holboellii complex (Brassicaceae). Heredity (Edinb) [Internet]. The Genetical Society of Great Britain. 2001;87(1):59–68.
   PubMedGoogle Scholar
• Burgess MB, Cushman KR, Doucette ET, et al. Effects of apomixis and polyploidy on diversification and geographic distribution in Amelanchier (Rosaceae). Am J Bot. 2014;101(8):1375–1387.
   PubMed Web of Science ®Google Scholar