Morphology, phylogenetic position, and mating behaviour of a new Mesobiotus (Tardigrada) species from a rock pool in the Socorro Box Canyon (New Mexico, USA)

References

• Bartels PJ Nelson DR. 2006. A large-scale, multihabitat inventory of the Phylum Tardigrada in the Great Smoky Mountains National Park, USA: A preliminary report. Hydrobiologia 558(1):111–118. DOI:10.1007/s10750-005-1405-9.
   Google Scholar
• Bartels PJ, Nelson DR Exline RP. 2011. Allometry and the removal of body size effects in the morphometric analysis of tardigrades. Journal of Zoological Systematics and Evolutionary Research 49(s1):17–25. DOI:10.1111/j.1439-0469.2010.00593.x.
   Google Scholar
• Beasley CW. 1988. Altitudinal distribution of Tardigrada of new Mexico with the description of a new species. The American Midland Naturalist 120(20):436–440. DOI:10.2307/2426016.
   Google Scholar
• Bertolani R. 1971. Contributo alla cariologia dei Tardigradi. Osservazioni su Macrobiotus hufelandii. Atti Della Accademia Nazionale dei Lincei: Classe di Scienze Fisiche, Matematiche e Naturali: Rendiconti 50(6):772–775.
   Google Scholar
• Bertolani R. 1981. A new genus and five new species of Italian freshwater tardigrades. Bollettino del Museo Civico di Storia Naturale di Verona 8:249–254.
   Google Scholar
• Bertolani R, Guidetti R, Marchioro T, Altiero T, Rebecchi L Cesari M. 2014. Phylogeny of Eutardigrada: New molecular data and their morphological support lead to the identification of new evolutionary lineages. Molecular Phylogenetics and Evolution 76(1):110–126. DOI:10.1016/j.ympev.2014.03.006.
   PubMedGoogle Scholar
• Binda MG Pilato G. 1994. Macrobiotus mottai, nuova specie di eutardigrado dell’Antartide. Animalia 21:53–56.
   Google Scholar
• Biserov VI. 1997/98. Tardigrades of the Caucasus with a taxonomic analysis of the genus Ramazzottius (Parachela: Hypsibiidae). Zoologischer Anzeiger 236:139–159.
   Web of Science ®Google Scholar
• Boix D, Kneitel J, Robson BJ, Duchet C, Zúñiga L, Day J, Gascón S, Sala J, Quintana XD Blaustein L. 2016. Invertebrates of freshwater temporary ponds in Mediterranean climates. In: Invertebrates in freshwater wetlands. Springer International Publishing. pp. 141–189. DOI:10.1007/978-3-319-24978-0_5.
   Google Scholar
• Brown PD, Schröder T, Ríos-Arana JV, Rico-Martinez R, Silva-Briano M, Wallace RL Walsh EJ. 2020. Patterns of Rotifer diversity in the Chihuahuan desert. Diversity 12(10):393. DOI:10.3390/d12100393.
   Google Scholar
• Casquet J, Thebaud C Gillespie RG. 2012. Chelex without boiling, a rapid and easy technique to obtain stable amplifiable DNA from small amounts of ethanol‐stored spiders. Molecular Ecology Resources 12(1):136–141. DOI:10.1111/j.1755-0998.2011.03073.x.
   PubMed Web of Science ®Google Scholar
• Coughlan K Stec D. 2019. Two new species of the Macrobiotus hufelandi complex (Tardigrada: Eutardigrada: Macrobiotidae) from Australia and India, with notes on their phylogenetic position. European Journal of Taxonomy 573:Article 573. DOI:10.5852/ejt.2019.573.
   Web of Science ®Google Scholar
• Darby BJ Neher DA. 2012. Stable isotope composition of microfauna supports the occurrence of biologically fixed nitrogen from cyanobacteria in desert soil food webs. Journal of Arid Environments 85:76–78. DOI: 10.1016/j.jaridenv.2012.06.006.
   Web of Science ®Google Scholar
• Davis H. 1873. A new Callidina: With the result of experiments on the desiccation of rotifers. The Monthly Microscopical Journal 9(5):201–209. DOI:10.1111/j.1365-2818.1873.tb02268.x.
   Google Scholar
• De Vries CH. 1996. Invertebrate community structure and dynamics in Korannaberg rock pools. Bloemfontein, South Africa: University of the Orange Free State.
   Google Scholar
• Degma P, and Guidetti R. 2023. Actual checklist of Tardigrada species (2009-2023, 42 th Edition: 09-01-2023). Modena, Italy: Università di Modena e Reggio Emilia.
   Google Scholar
• Doyère L. 1840. Mémoire sur les Tardigrades. Annales des Sciences Naturelles 14:269–361.
   Google Scholar
• Edgar RC. 2004. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32(5):1792–1797. DOI:10.1093/nar/gkh340.
   PubMed Web of Science ®Google Scholar
• Esri. 2017. Topographic [basemap]. Scale not given. “World topographic Map”. Oct 26. Available: https://esri.maps.arcgis.com/home/item.html?id=7dc6cea0b1764a1f9af2e679f642f0f5. Accessed Mar 2023 9.
   Google Scholar
• Friard O Gamba M. 2016. BORIS: A free, versatile open‐source event‐logging software for video/audio coding and live observations. Methods in Ecology and Evolution 7(11):1325–1330. DOI:10.1111/2041-210X.12584.
   Web of Science ®Google Scholar
• GIMP Development Team. 2019. GIMP. Available: https://www.gimp.org.
   Google Scholar
• Guidetti R, Massa E, Bertolani R, Rebecchi L Cesari M. 2019. Increasing knowledge of Antarctic biodiversity: New endemic taxa of tardigrades (Eutardigrada; Ramazzottiidae) and their evolutionary relationships. Systematics and Biodiversity 17(6):573–593. DOI:10.1080/14772000.2019.1649737.
   Web of Science ®Google Scholar
• Guidetti R, Schill RO, Bertolani R, Dandekar T Wolf M. 2009. New molecular data for tardigrade phylogeny, with the erection of Paramacrobiotus gen. nov. Journal of Zoological Systematics and Evolutionary Research 47(4):315–321. DOI:10.1111/j.1439-0469.2009.00526.x.
   Web of Science ®Google Scholar
• Guidi A Rebecchi L. 1996. Spermatozoan morphology as a character for tardigrade systematics: Comparison with sclerified parts of animals and eggs in eutardigrades. Zoological Journal of the Linnean Society 116(1–2):101–113. DOI:10.1111/j.1096-3642.1996.tb02336.x.
   Web of Science ®Google Scholar
• Hengherr S Schill RO. 2018. Environmental adaptations: Cryobiosis. In: Schill R, editor. Water bears: The biology of Tardigrades. Zoological monographs. Vol. 2. Cham: Springer International Publishing. pp. 295–310. DOI:10.1007/978-3-319-95702-9_11.
   Google Scholar
• Hortal J, de Bello F, Diniz-Filho JAF, Lewinsohn TM, Lobo JM Ladle RJ. 2015. Seven shortfalls that beset large-scale knowledge of biodiversity. Annual Review of Ecology, Evolution, and Systematics 46(1):523–549. DOI:10.1146/annurev-ecolsys-112414-054400.
   Web of Science ®Google Scholar
• Inkscape Project. 2020. Inkscape. Available: https://inkscape.org.
   Google Scholar
• Itang LAM, Stec D, Mapalo MA, Mirano-Bascos D Michalczyk Ł. 2020. An integrative description of Mesobiotus dilimanensis, a new tardigrade species from the Philippines (Eutardigrada: Macrobiotidae: Furciger group). The Raffles Bulletin of Zoology 68:19–31.
   Web of Science ®Google Scholar
• Jocqué M, Graham T Brendonck L. 2007. Local structuring factors of invertebrate communities in ephemeral freshwater rock pools and the influence of more permanent water bodies in the region. Hydrobiologia 592(1):271–280. DOI:10.1007/s10750-007-0766-7.
   Google Scholar
• Jornada Basin Spatial Data Laboratory, NSF Jornada Basin LTER/USDA ARS Jornada Experimental Range. 2006 Mar. Chihuahuan desert boundary. Available: http://jornada-www.nmsu.edu/gis/giscat.php. Accessed Apr 2022 21.
   Google Scholar
• Kaczmarek Ł, Bartylak T, Stec D, Kulpa A, Kepel M, Kepel A Roszkowska M. 2020. Revisiting the genus Mesobiotus (Eutardigrada, Macrobiotidae)–remarks, updated dichotomous key and an integrative description of new species from Madagascar. Zoologischer Anzeiger 287:121–146. DOI:10.1016/j.jcz.2020.05.003.
   Web of Science ®Google Scholar
• Kaczmarek Ł, Michalczyk Ł McInnes SJ. 2016. Annotated zoogeography of non-marine Tardigrada. Part III: North America and Greenland. Zootaxa 4203(1):1–249. DOI:10.11646/zootaxa.4203.1.1.
   Web of Science ®Google Scholar
• Kaczmarek Ł, Zawierucha K, Buda J, Stec D, Gawlak M, Michalczyk Ł, Roszkowska M, and Rubal M. 2018. An integrative redescription of the nominal taxon for the Mesobiotus harmsworthi group (Tardigrada: Macrobiotidae) leads to descriptions of two new Mesobiotus species from Arctic. PLoS One 13(10):e0204756. DOI:10.1371/journal.pone.0204756.
   PubMed Web of Science ®Google Scholar
• Katoh K. 2002. MAFFT: A novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30(14):3059–3066. DOI:10.1093/nar/gkf436.
   PubMed Web of Science ®Google Scholar
• Katoh K Toh H. 2008. Recent developments in the MAFFT multiple sequence alignment program. Briefings in Bioinformatics 9(4):286–298. DOI:10.1093/bib/bbn013.
   PubMed Web of Science ®Google Scholar
• Kayastha P, Roszkowska M, Mioduchowska M, Gawlak M, and Kaczmarek Ł. 2021. Integrative descriptions of two new tardigrade species along with the new record of Mesobiotus skorackii Kaczmarek et al., 2018 from Canada. Diversity 13(8):394. DOI:10.3390/d13080394.
   Google Scholar
• Koste W. 1996. On soil Rotatoria from a lithotelma near Halali Lodge in Etosha National Park in N-Namibia, South Africa. Internationale Revue Der Gesamten Hydrobiologie Und Hydrographie 81(3):353–365. DOI:10.1002/iroh.19960810305.
   Google Scholar
• Lanfear R, Frandsen PB, Wright AM, Senfeld T, and Calcott B. 2016. PartitionFinder 2: New Methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution msw260. DOI: 10.1093/molbev/msw260.
   Web of Science ®Google Scholar
• Mapalo MA, Stec D, Mirano-Bascos D Michalczyk Ł. 2016. Mesobiotus philippinicus sp. nov., the first limnoterrestrial tardigrade from the Philippines. Zootaxa 4126(3):Article 3. DOI:10.11646/zootaxa.4126.3.6.
   Web of Science ®Google Scholar
• Mapalo MA, Stec D, Mirano-Bascos D, and Michalczyk Ł. 2017. An integrative description of a limnoterrestrial tardigrade from the Philippines, Mesobiotus insanis, new species (Eutardigrada: Macrobiotidae: Harmsworthi group). The Raffles Bulletin of Zoology 65:440–454.
   Web of Science ®Google Scholar
• Marley NJ, Mcinnes SJ Sands CJ. 2011. Phylum Tardigrada: A re-evaluation of the Parachela. Zootaxa 64(2819):Article 2819. DOI:10.11646/zootaxa.2819.1.2.
   Google Scholar
• Maucci W. 1991. Tre nuove specie di Eutardigradi della Groenlandia Meridionale. Bollettino del Museo Civico di Storia Naturale di Verona 15:279–286.
   Google Scholar
• Maucci W Durante-Pasa M. 1984. Macrobiotus lusitanicus sp. nov., nuova pecie di Eutardigrado del Portogallo nord-occidentale (Tardigrada, Macrobiotidae). Bollettino del Museo Civico di Storia Naturale di Verona 11:319–326.
   Google Scholar
• McDaniel J. 2022. Chihuahuan desert rock pool community assemblages: Patterns of taxonomic diversity. El Paso, Texas, USA: University of Texas at El Paso.
   Google Scholar
• Mehlen RH. 1969. New Tardigrada from Texas. The American Midland Naturalist 81(2):395–404. DOI:10.2307/2423979.
   Web of Science ®Google Scholar
• Meyer HA. 2013. Terrestrial and freshwater Tardigrada of the Americas. Zootaxa 3747(1):1–71. DOI:10.11646/zootaxa.3747.1.1.
   PubMed Web of Science ®Google Scholar
• Meyer HA Hinton JG. 2010. Milnesium zsalakoae and M. jacobi, two new species of Tardigrada (Eutardigrada: Apochela: Milnesiidae) from the southwestern United States. Proceedings of the Biological Society of Washington 123(2):113–120. DOI:10.2988/09-29.1.
   Web of Science ®Google Scholar
• Murray J. 1907. XXV.—Arctic Tardigrada, collected by Wm. S. Bruce. Earth and Environmental Science Transactions of the Royal Society of Edinburgh 45(3):669–681. DOI:10.1017/S0080456800011789.
   Google Scholar
• Murray J. 1910. Tardigrada. In: British Antarctic expedition 1907—1909: Reports on the scientific Investigations: Vol. I: Biology, Part V. pp. 115–256.
   Google Scholar
• Mutterer J Zinck E. 2013. Quick-and-clean article figures with FigureJ. Journal of Microscopy 252(1):89–91. DOI:10.1111/jmi.12069.
   PubMed Web of Science ®Google Scholar
• Nelson DR, Bartels PJ Guil N. 2018. Tardigrade Ecology. In: Schill R, editor. Water bears: The biology of Tardigrades. Zoological monographs. Vol. 2. Cham: Springer International Publishing. pp. 163–210. DOI:10.1007/978-3-319-95702-9_7.
   Google Scholar
• Nowak B Stec D. 2018. An integrative description of Macrobiotus hannae sp. nov. (Tardigrada: Eutardigrada: Macrobiotidae: Hufelandi group) from Poland. Turkish Journal of Zoology 42(3):Article 3. DOI:10.3906/zoo-1712-31.
   Web of Science ®Google Scholar
• Pilato G. 1981. Analisi di nuovi caratteri nello studio degli Eutardigradi. Animalia 8:51–57.
   Google Scholar
• Pilato G, Paulo F Lisi O. 2007. Remarks on the Echiniscus viridis group, with the description of a new species (Tardigrada, Echiniscidae). Journal of Limnology 66:33–39. DOI:10.4081/jlimnol.2007.s1.33.
   Web of Science ®Google Scholar
• Rambaut A. 2007. FigTree, a graphical viewer of phylogenetic trees. http://tree.bio.ed.ac.uk/software/figtree/.
   Google Scholar
• Rambaut A, Drummond AJ, Xie D, Baele G, Suchard MA Susko E. 2018. Posterior summarization in bayesian phylogenetics using Tracer 1.7. Systematic Biology 67(5):901–904. DOI:10.1093/sysbio/syy032.
   PubMed Web of Science ®Google Scholar
• Rebecchi L. 1997. Ultrastructural study of spermiogenesis and the testicular and spermathecal spermatozoon of the gonochoristic tardigrade Xerobiotus pseudohufelandi (Eutardigrada, Macrobiotidae). Journal of Morphology 234(1):11–24. DOI:10.1002/(SICI)1097-4687(199710)234:1<11:AID-JMOR2>3.0.CO;2-Q.
   PubMed Web of Science ®Google Scholar
• Rebecchi L, Altiero T Guidetti R. 2007. Anhydrobiosis: The extreme limit of desiccation tolerance. Invertebrate Survival Journal 4(2):65–81.
   Web of Science ®Google Scholar
• Rebecchi L, Altiero L Guidi A. 2011. The ultrastructure of the tardigrade spermatozoon: A comparison between Paramacrobiotus and Macrobiotus species (Eutardigrada). Invertebrate Zoology 8(1):63–67. DOI:10.15298/invertzool.08.1.08.
   Google Scholar
• Rebecchi L Bertolani R. 1994. Maturative pattern of ovary and testis in eutardigrades of freshwater and terrestrial habitats. Invertebrate Reproduction & Development 26(2):107–117. DOI:10.1080/07924259.1994.9672407.
   Web of Science ®Google Scholar
• Rebecchi L Guidi A. 1991. First SEM studies on tardigrade spermatozoa. Invertebrate Reproduction & Development 19(2):151–156. DOI:10.1080/07924259.1991.9672169.
   Web of Science ®Google Scholar
• Rebecchi L, Guidi A, and Bertolani R. 2000. Tardigrada. In: Adiyodi KG, Adiyodi RG, editors. Reproductive biology of invertebrates. Progress in male gamete ultrastructure and phylogenyOxford & IBH Publishing CO PVT LTD. Vol. 9. pp. 267–291.
   Google Scholar
• Richters F. 1926. Tardigrada. In: Kükenthal W, and Krumbach, editors. Handbuch der Zoologie. Vol. 3. Berlin, Germany: T. Walter de Gruyter & Co. pp. 58–61.
   Google Scholar
• Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA Huelsenbeck JP. 2012. MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3):539–542. DOI:10.1093/sysbio/sys029.
   PubMed Web of Science ®Google Scholar
• Roszkowska M, Stec D, Gawlak M Kaczmarek Ł. 2018. An integrative description of a new tardigrade species Mesobiotus romani sp. nov. (Macrobiotidae: Harmsworthi group) from the Ecuadorian Pacific coast. Zootaxa 4450(5):Article 5. DOI:10.11646/zootaxa.4450.5.2.
   Web of Science ®Google Scholar
• Schill RO Hengherr S. 2018. Environmental adaptations: Desiccation tolerance. In: Schill, R., editor. Water bears: The biology of Tardigrades. Zoological monographs. Vol. 2. Cham: Springer International Publishing. pp. 273–293. DOI:10.1007/978-3-319-95702-9_10.
   Google Scholar
• Schneider CA, Rasband WS Eliceiri KW. 2012. NIH image to ImageJ: 25 years of image analysis. Nature Methods 9(7):671–675. DOI:10.1038/nmeth.2089.
   PubMed Web of Science ®Google Scholar
• Schröder T, Howard S, Arroyo ML Walsh EJ. 2007. Sexual reproduction and diapause of Hexarthra sp. (Rotifera) in short-lived ponds in the Chihuahuan desert. Freshwater Biology 52(6):1033–1042. DOI:10.1111/j.1365-2427.2007.01751.x.
   Web of Science ®Google Scholar
• Schuster RO, Nelson DR, Grigarick AA Christenberry D. 1980. Systematic criteria of the Eutardigrada. Transactions of the American Microscopical Society 99(3):284. DOI:10.2307/3226004.
   Google Scholar
• Short KA, Sands CJ, McInnes SJ, Pisani D, Stevens MI Convey P. 2022. An ancient, antarctic-specific species complex: Large divergences between multiple antarctic lineages of the tardigrade genus Mesobiotus. Molecular Phylogenetics and Evolution 170:107429. DOI:10.1016/j.ympev.2022.107429.
   PubMedGoogle Scholar
• Spencer M, Blaustein L, Schwartz S Cohen J. 1999. Species richness and the proportion of predatory animal species in temporary freshwater pools: Relationships with habitat size and permanence. Ecology Letters 2:157–166. DOI:10.1046/j.1461-0248.1999.00062.x.
   Web of Science ®Google Scholar
• Stec D. 2019. Mesobiotus datanlanicus sp. nov., a new tardigrade species (Macrobiotidae: Mesobiotus harmsworthi group) from Lâm ồng Province in Vietnam. Zootaxa 4679(1):Article 1. DOI:10.11646/zootaxa.4679.1.10.
   Web of Science ®Google Scholar
• Stec D. 2021. Integrative descriptions of two new Mesobiotus species (Tardigrada, Eutardigrada, Macrobiotidae) from Vietnam. Diversity 13(11):605. DOI:10.3390/d13110605.
   Google Scholar
• Stec D. 2022. Integrative descriptions of two new Mesobiotus species (Tardigrada, Eutardigrada, Macrobiotidae) from Vietnam. Zoological Studies 61:605. DOI:10.6620/ZS.2022.61-85.
   Google Scholar
• Stec D Kristensen RM. 2017. An integrative description of Mesobiotus ethiopicus sp. nov.(Tardigrada: Eutardigrada: Parachela: Macrobiotidae: Harmsworthi group) from the Northern Afrotropic region. Turkish Journal of Zoology 41(5):Article 5. DOI:10.3906/zoo-1701-47.
   Web of Science ®Google Scholar
• Stec D, Kristensen RM, and Michalczyk Ł. 2020. An integrative description of minibiotus ioculator sp. nov. from the Republic of South Africa with notes on Minibiotus pentannulatus Londoño et al., 2017 (Tardigrada: Macrobiotidae). Zoologischer Anzeiger 286:117–134. DOI:10.1016/j.jcz.2020.03.007.
   Web of Science ®Google Scholar
• Stec D, and Morek W. 2022. Reaching the monophyly: Re-evaluation of the enigmatic species Tenuibiotus hyperonyx (Maucci, 1983) and the genus Tenuibiotus (Eutardigrada). Animals 12(3):404. DOI:10.3390/ani12030404.
   Web of Science ®Google Scholar
• Stec D, Roszkowska M, Kaczmarek Ł Michalczyk Ł. 2018. An integrative description of a population of Mesobiotus radiatus (Pilato, Binda & Catanzaro, 1991) from Kenya. Turkish Journal of Zoology 42(5):Article 5. DOI:10.3906/zoo-1802-43.
   Web of Science ®Google Scholar
• Stec D, Vecchi M, Calhim S Michalczyk Ł. 2021. New multilocus phylogeny reorganises the family Macrobiotidae (Eutardigrada) and unveils complex morphological evolution of the Macrobiotus hufelandi group. Molecular Phylogenetics and Evolution 160:160. DOI:10.1016/j.ympev.2020.106987.
   Web of Science ®Google Scholar
• Stec D, Vončina K, Møbjerg Kristensen R Michalczyk Ł. 2022. The Macrobiotus ariekammensis species complex provides evidence for parallel evolution of claw elongation in macrobiotid tardigrades. Zoological Journal of the Linnean Society 195(4):1067–1099. DOI:10.1093/zoolinnean/zlab101.
   Web of Science ®Google Scholar
• Sugiura K Matsumoto M. 2021a. Reproduction of Mesobiotus: Comparison of morphology and behavior in the family Macrobiotidae (Tardigrada: Eutardigrada). Zoological Science 38(5). DOI:10.2108/zs210045.
   PubMed Web of Science ®Google Scholar
• Sugiura K Matsumoto M. 2021b. Sexual reproductive behaviours of tardigrades: A review. Invertebrate Reproduction & Development 65(4):279–287. DOI:10.1080/07924259.2021.1990142.
   Web of Science ®Google Scholar
• Sugiura K Matsumoto M. 2021c. Spermatozoa morphology changes during reproduction and first observation of acrosomal contact in two dioecious species of Macrobiotidae (Tardigrada: Eutardigrada). Zygote 29(1):42–48. DOI:10.1017/S0967199420000490.
   PubMed Web of Science ®Google Scholar
• Thulin G. 1928. Über die Phylogenie und das System der Tardigraden. Hereditas 11(2–3):207–266. DOI:10.1111/j.1601-5223.1928.tb02488.x.
   Google Scholar
• Tumanov DV. 2005. Two new species of Macrobiotus (Eutardigrada, Macrobiotidae) from Tien Shan (Kirghizia), with notes on Macrobiotus tenuis group. Zootaxa 46(1043):Article 1043. DOI:10.11646/zootaxa.1043.1.3.
   Google Scholar
• Tumanov DV. 2020. Integrative description of Mesobiotus anastasiae sp. nov. (Eutardigrada, Macrobiotoidea) and first record of Lobohalacarus (Chelicerata, Trombidiformes) from the Republic of South Africa. European Journal of Taxonomy 726. DOI: 10.5852/ejt.2020.726.1179.
   Web of Science ®Google Scholar
• Vecchi M Bruneaux M. 2021. Concatipede: An R package to concatenate fasta sequences easily. DOI:10.5281/zenodo.5130604.
   Google Scholar
• Vecchi M, Cesari M, Bertolani R, Ingemar Jönsson K, Rebecchi L Guidetti R. 2016. Integrative systematic studies on tardigrades from Antarctica identify new genera and new species within Macrobiotoidea and Echiniscoidea. Invertebrate Systematics 30(4):303. DOI:10.1071/IS15033.
   Web of Science ®Google Scholar
• Vecchi M, Ferrari C, Stec D Calhim S. 2022. Desiccation risk favours prevalence and diversity of tardigrade communities and influences their trophic structure in alpine ephemeral rock pools. Hydrobiologia 849(9):1995–2007. DOI:10.1007/s10750-022-04820-0.
   Web of Science ®Google Scholar
• Vecchi M Stec D. 2021. Integrative descriptions of two new Macrobiotus species (Tardigrada, Eutardigrada, Macrobiotidae) from Mississippi (USA) and Crete (Greece). Zoosystematics and Evolution 97(1):381–306. DOI:10.3897/zse.97.65280.
   Web of Science ®Google Scholar
• Vecchi M, Tsvetkova A, Stec D, Ferrari C, Calhim S Tumanov D. 2023. Expanding Acutuncus: Phylogenetics and morphological analyses reveal a considerably wider distribution for this tardigrade genus. Molecular Phylogenetics and Evolution 180:107707. DOI:10.1016/j.ympev.2023.107707.
   PubMed Web of Science ®Google Scholar
• Velasco-González I, Sanchez-Jimenez A, Singer D, Murciano A, Díez-Hermano S, Lara E Martín-Cereceda M. 2020. Rain-fed granite rock basins accumulate a high diversity of dormant microbial eukaryotes. Microbial Ecology 79(4):882–897. DOI:10.1007/s00248-019-01463-y.
   PubMed Web of Science ®Google Scholar
• Zawierucha K, Ostrowska M, Vonnahme TR, Devetter M, Nawrot AP, Lehmann S Kolicka M. 2016. Diversity and distribution of Tardigrada in Arctic cryoconite holes. Journal of Limnology 75(3). DOI:10.4081/jlimnol.2016.1453.
   Web of Science ®Google Scholar
• Zawierucha K, Stec D, Lachowska-Cierlik D, Takeuchi N, Li Z Michalczyk Ł. 2018. High mitochondrial Diversity in a new water bear species (Tardigrada: Eutardigrada) from Mountain Glaciers in Central Asia, with the erection of a new genus Cryoconicus. Annales Zoologici 68(1):179–201. DOI:10.3161/00034541anz2018.68.1.007.
   Web of Science ®Google Scholar