Screening for pancreatic lipase inhibitors: evaluating assay conditions using p-nitrophenyl palmitate as substrate

References

• Abdul Rahman H, Saari N, Abas F, Ismail A, Mumtaz MW, Abdul Hamid A. 2017. Anti-obesity and antioxidant activities of selected medicinal plants and phytochemical profiling of bioactive compounds. Int J Food Prop. 20(11):2616–2629.
   Web of Science ®Google Scholar
• Bagi K, Simon LM, Szajani B. 1997. Immobilization and characterization of porcine pancreas lipase. Enzyme Microb Technol. 20(7):531–535.
   Web of Science ®Google Scholar
• Bai Y-X, Li Y-F, Yang Y, Yi L-X. 2006. Covalent immobilization of triacylglycerol lipase onto functionalized novel mesoporous silica supports. J Biotechnol. 125(4):574–582.
   PubMed Web of Science ®Google Scholar
• Bautista FM, Bravo MC, Campelo JM, García A, Luna D, Marinas JM, Romero AA. 1998. Covalent immobilization of porcine pancreatic lipase on amorphous AlPO4 and other inorganic supports. J Chem Technol Biotechnol. 72(3):249–254.
   Web of Science ®Google Scholar
• Bialecka-Florjanczyk E, Fabiszewska AU, Krzyczkowska J, Kurylowicz A. 2018. Synthetic and natural lipase inhibitors. Mini-Rev Med Chem. 18(8):672–683.
   PubMed Web of Science ®Google Scholar
• Birari RB, Bhutani KK. 2007. Pancreatic lipase inhibitors from natural sources: unexplored potential. Drug Discov Today. 12(19-20):879–889.
   PubMed Web of Science ®Google Scholar
• Bisswanger H. 2014. Enzyme assays. Perspect Sci. 1(1-6):41–55.
   Google Scholar
• Buchholz T, Melzig M. 2015. Polyphenolic compounds as pancreatic lipase inhibitors. Planta Med. 81(10):771–783.
   PubMed Web of Science ®Google Scholar
• Carrière F, Renou C, Lopez V, De Caro J, Ferrato F, Lengsfeld H, De Caro A, Laugier R, Verger R. 2000. The specific activities of human digestive lipases measured from the in vivo and in vitro lipolysis of test meals. Gastroenterology. 119(4):949–960.
   PubMed Web of Science ®Google Scholar
• Chauhan D, George G, Sridhar SNC, Bhatia R, Paul AT, Monga V. 2019. Design, synthesis, biological evaluation, and molecular modeling studies of rhodanine derivatives as pancreatic lipase inhibitors. Archiv der Pharmazie. 352(10):1900029.
   Web of Science ®Google Scholar
• Daneschvar HL, Aronson MD, Smetana GW. 2016. FDA-approved anti-obesity drugs in the United States. Am J Med. 129(8):e871–e879.
   Web of Science ®Google Scholar
• de la Garza AL, Milagro FI, Boque N, Campión J, Martínez JA. 2011. Natural inhibitors of pancreatic lipase as new players in obesity treatment. Planta Med. 77(8):773–785.
   PubMed Web of Science ®Google Scholar
• Di L, Kerns EH. 2006. Biological assay challenges from compound solubility: strategies for bioassay optimization. Drug Discov Today. 11(9-10):446–451.
   PubMed Web of Science ®Google Scholar
• Emimmal MES, Sankar V. 2019. Synthesis and pancreatic lipase inhibitory activity of phenacyl esters of n-aroyl amino acids. Curr Enzyme Inhib. 15(2):133–143.
   Google Scholar
• Freitas L, Bueno T, Perez VH, Santos JC, de Castro HF. 2007. Enzymatic hydrolysis of soybean oil using lipase from different sources to yield concentrated of polyunsaturated fatty acids. World J Microbiol Biotechnol. 23(12):1725–1731.
   PubMed Web of Science ®Google Scholar
• Gilham D, Lehner R. 2005. Techniques to measure lipase and esterase activity in vitro. Methods. 36(2):139–147.
   PubMed Web of Science ®Google Scholar
• Gupta N, Rathi P, Gupta R. 2002. Simplified para-nitrophenyl palmitate assay for lipases and esterases. Anal Biochem. 311(1):98–99.
   PubMed Web of Science ®Google Scholar
• Jeong JY, Jo YH, Kim SB, Liu Q, Lee JW, Mo EJ, Lee KY, Hwang BY, Lee MK. 2015. Pancreatic lipase inhibitory constituents from morus alba leaves and optimization for extraction conditions. Bioorg Med Chem Lett. 25(11):2269–2274.
   PubMed Web of Science ®Google Scholar
• Kordel M, Hofmann B, Schomburg D, Schmid RD. 1991. Extracellular lipase of Pseudomonas sp. strain ATCC 21808: purification, characterization, crystallization, and preliminary X-ray diffraction data. J Bacteriol. 173(15):4836–4841.
   PubMed Web of Science ®Google Scholar
• Kwon C-S, Sohn HY, Kim SH, Kim JH, Son KH, Lee JS, Lim JK, Kim J-S. 2003. Anti-obesity effect of Dioscorea nipponica makino with lipase-inhibitory activity in rodents. Biosci Biotechnol Biochem. 67(7):1451–1456.
   PubMed Web of Science ®Google Scholar
• Lerner H, Mehler L. 2019. FDA approval and surveillance process for anti-obesity drugs and devices. In: Morton JM, Brethauer SA, DeMaria EJ, Kahan S, Hutter MM, editors. Quality in obesity treatment. Cham: Springer International Publishing; p. 375–383.
   Google Scholar
• Lewis DR, Liu DJ. 2012. Direct measurement of lipase inhibition by orlistat using a dissolution linked in vitro assay. Clin Pharmacol Biopharma. 1. doi:10.4172/2167-065X.1000103.
   PubMedGoogle Scholar
• Li Y, Hu M, McClements DJ. 2011. Factors affecting lipase digestibility of emulsified lipids using an in vitro digestion model: proposal for a standardised pH-stat method. Food Chem. 126(2):498–505.
   Web of Science ®Google Scholar
• Lowe ME. 1999. Assays for pancreatic triglyceride lipase and colipase. Methods Mol Biol. 109:59–70.
   PubMedGoogle Scholar
• Mateos-Díaz E, Rodríguez JA, de los Ángeles Camacho-Ruiz M, Mateos-Díaz JC. 2012. High-throughput screening method for lipases/esterases. In: Sandoval G, editor. Lipases and phospholipases: Methods and protocols. Totowa, NJ: Humana Press; p. 89–100.
   Google Scholar
• Mendes AA, Oliveira PC, de Castro HF. 2012. Properties and biotechnological applications of porcine pancreatic lipase. J Mol Catal B: Enzym. 78:119–134.
   Web of Science ®Google Scholar
• Menteşe E, Karaali N, Akyüz G, Yılmaz F, Ülker S, Kahveci B. 2016. Synthesis and evaluation of α-glucosidase and pancreatic lipase inhibition by quinazolinone-coumarin hybrids. Chem Heterocycl Compd. 52(12):1017–1024.
   Web of Science ®Google Scholar
• Naka Y, Nakamura T. 1994. Effects of colipase, bile salts, Na+, and Ca2+ on pancreatic lipase activity. Biosci Biotechnol Biochem. 58(11):2121–2122.
   PubMed Web of Science ®Google Scholar
• Newman DJ, Cragg GM. 2012. Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod. 75(3):311–335.
   PubMed Web of Science ®Google Scholar
• Ninomiya K, Matsuda H, Shimoda H, Nishida N, Kasajima N, Yoshino T, Morikawa T, Yoshikawa M. 2004. Carnosic acid, a new class of lipid absorption inhibitor from sage. Bioorg Med Chem Lett. 14(8):1943–1946.
   PubMed Web of Science ®Google Scholar
• Obesity and Overweight [Internet]: World Health Organization (WHO) [posted June 9, 2021]; [cited Jun 15, 2021]. https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight.
   Google Scholar
• Pinsirodom P, Parkin KL. 2001. Lipase assays. Curr Protoc Food Anal Chem. 00(1):C3.1.1–C3.1.13.
   Google Scholar
• Plou FJ, Ferrer M, Nuero OM, Calvo MV, Alcalde M, Reyes F, Ballesteros A. 1998. Analysis of tween 80 as an esterase/lipase substrate for lipolytic activity assay. Biotechnol Tech. 12(3):183–186.
   Google Scholar
• Pohanka M. 2019. Biosensors and bioassays based on lipases, principles and applications, a review. Molecules. 24(3):616.
   PubMed Web of Science ®Google Scholar
• Popkin BM, Du S, Green WD, Beck MA, Algaith T, Herbst CH, Alsukait RF, Alluhidan M, Alazemi N, Shekar M. 2020. Individuals with obesity and COVID-19: A global perspective on the epidemiology and biological relationships. Obes Rev. 21(11):e13128.
   PubMed Web of Science ®Google Scholar
• Rajan L, Palaniswamy D, Mohankumar SK. 2020. Targeting obesity with plant-derived pancreatic lipase inhibitors: A comprehensive review. Pharmacol Res. 155:104681.
   PubMed Web of Science ®Google Scholar
• Schrauwen P, Westerterp KR. 2000. The role of high-fat diets and physical activity in the regulation of body weight. Br J Nutr. 84(4):417–427.
   PubMed Web of Science ®Google Scholar
• Scopes RK. 1993. Protein purification: principles and practice. 2nd ed. New York: Springer Science & Business Media. Chapter 9: Measurement of enzyme activity: 253-254.
   Google Scholar
• Sergent T, Vanderstraeten J, Winand J, Beguin P, Schneider Y-J. 2012. Phenolic compounds and plant extracts as potential natural anti-obesity substances. Food Chem. 135(1):68–73.
   Web of Science ®Google Scholar
• Sridhar S, Bhurta D, Kantiwal D, George G, Monga V, Paul AT. 2017. Design, synthesis, biological evaluation and molecular modelling studies of novel diaryl substituted pyrazolyl thiazolidinediones as potent pancreatic lipase inhibitors. Bioorg Med Chem Lett. 27(16):3749–3754.
   PubMed Web of Science ®Google Scholar
• Sridhar S, Ginson G, Reddy PV, Tantak MP, Kumar D, Paul AT. 2017. Synthesis, evaluation and molecular modelling studies of 2-(carbazol-3-yl)-2-oxoacetamide analogues as a new class of potential pancreatic lipase inhibitors. Bioorg Med Chem. 25(2):609–620.
   PubMed Web of Science ®Google Scholar
• Sridhar SNC, George G, Verma A, Paul AT. 2019. Natural products-based pancreatic lipase inhibitors for obesity treatment. In: Akhtar MS, Swamy MK, Sinniah UR, editors. Natural bio-active compounds. Vol. 1: Production and applications. Singapore: Springer Singapore. p. 149–191.
   Google Scholar
• Vasudevan PT, López-Cortés N, Caswell H, Reyes-Duarte D, Plou FJ, Ballesteros A, Como K, Thomson T. 2004. A novel hydrophilic support, cofoam, for enzyme immobilization. Biotechnol Lett. 26(6):473–477.
   PubMed Web of Science ®Google Scholar
• Winkler F, d'Arcy A, Hunziker W. 1990. Structure of human pancreatic lipase. Nature. 343:771–774.
   PubMed Web of Science ®Google Scholar
• Winkler UK, Stuckmann M. 1979. Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by serratia marcescens. J Bacteriol. 138(3):663–670.
   PubMed Web of Science ®Google Scholar
• Zou B, Hu Y, Yu D, Xia J, Tang S, Liu W, Huang H. 2010. Immobilization of porcine pancreatic lipase onto ionic liquid modified mesoporous silica sba-15. Biochem Eng J. 53(1):150–153.
   Web of Science ®Google Scholar