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Research Article

DEVELOPMENT AND UTILIZATION OF PRIMARY HEPATOCYTE CULTURE SYSTEMS TO EVALUATE METABOLISM, DNA BINDING, AND DNA REPAIR OF XENOBIOTICS*

Pages 1-13 | Published online: 15 Apr 2001

REFERENCES

  • Bonney R. J., Walker P. R., Potter V. R. Isozyme patterns in parenchymal and non-parenchymal cells isolated from regenerating and regenerated rat liver. Biochem. J. 1974; 32: 947–954
  • Bonney R. J., Becker J. E., Walker P. R., Potter V. R. Adult liver parenchymal cells in primary culture characteristics and standards. In Vitro 1974; 10: 130–142
  • Seglen P. O. Preparation of isolated rat liver cells. Methods Cell Biol. 1976; 13: 29–83
  • Casciano D. A. Use of isolated rodent hepatocytes to evaluate potential premutagens and precarcinogens. Banbury Report 2, A. W. Hsie, J. P. O'Neill, V. K. McElheny. Cold Spring Harbor Press, Cold Spring Harbor, NY 1979; 125–130
  • Williams G. M. Carcinogen-induced DNA repair in primary rat liver cell cultures; a possible screen for chemical carcinogens. Cancer Lett. 1976; 1: 231–235
  • Casciano D. A., Farr J. A., Oldham J. W., Cave M. D. 2-Acetylaminofluorene-induced unscheduled DNA synthesis in hepatocytes isolated from 3-methylcholanthrene treated rats. Cancer Lett. 1978; 5: 173–178
  • Michalopoulos G., Sattler G., O'Connor L., Pitot H. C. Unscheduled DNA synthesis induced by procarcinogens in suspensions and primary cultures of hepatocytes on collagen membranes. Cancer Res. 1978; 38: 1866–1871
  • Yager J. D., Miller J. A. DNA repair in primary cultures of rat hepatocyes. Cancer Res. 1978; 38: 4385–4394
  • Oldham J. W., Casciano D. A., Cave M. D. Comparative induction of unscheduled DNA synthesis by physical and chemical agents in non-proliferating primary cultures of rat hepatocytes. Chem.-Biol. Interact. 1980; 20: 303–314
  • Hass B. S., Heflich R. H., Shaddock J. G., Casciano D. A. Evidence for a preinduction period of less than 5 days for aroclor pretreatment of rat liver in hepatocyte mediation of benzo[a]pyrene and 2-aminofluorene mutagenicity in Salmonella strain TA98. Environ. Mutagen. 1985; 7: 381–403
  • Hass B. S., Heflich R. H., Chou M. W., White G. L., Fu P. P., Casciano D. A. Hepatocyte-mediated mutagenicity of mononitrobenzo[a]pyrenes in Salmonella typhimurium. Mutat. Res. 1986; 171: 123
  • Olson M. J., Casciano D. A., Pounds J. G. Potentiation of dimethylnitrosamine genotoxicity in rat hepatocytes following ethanol treatment in vivo. Chem.-Biol. Interact. 1984; 50: 313–326
  • McMillan D. C., Shaddock J. G., Heflich R. H., Casciano D. A., Hinson J. A. Evaluation of propanil and its N-oxidized derivatives in Salmonella typhimurium reversion, Chinese hamster ovary/hypoxanthine guanine phosphoribosyl transferase, and hepatocyte/DNA repair assays. Fundam. Appl. Toxicol. 1988; 11: 429–439
  • Shaddock J. G., Robinson B. Y., Casciano D. A. Effect of pretreatment with hepatic mixed function oxidase inducers on the genotoxicity of four rat carcinogens in the hepatocyte/DNA repair assay. Mutagenesis 1990; 5: 387–391
  • Casciano D. A., Gaylor D. W. Statistical criteria for evaluating chemicals as positive or negative in the hepatocyte/DNA repair assay. Mutat. Res. 1983; 122: 81–86
  • Williams G. M. Detection of chemical carcinogens by unscheduled DNA synthesis in rat liver primary cell cultures. Cancer Res. 1977; 37: 1845–1851
  • Shaddock J. G., Feuers R. J., Chou M. W., Pegram R. A., Casciano D. A. Effects of aging and caloric restriction on genotoxicity of four carcinogens in the in vitro rat hepatocyte/DNA repair assay. Mutat. Res. 1993; 295: 19–30
  • Shaddock J. G., Feuers R. J., Chou M. W., Casciano D. A. Evidence that DNA repair may not be modified by age or caloric restriction. Mutat. Res. 1993; 301: 261–266
  • Shaddock J. G., Feuers R. J., Casciano D. A. Aging and enzyme induction: Effect on chemically-induced DNA repair in non-proliferating Fischer 344 hepatocytes. Age 1993; 18: 105–111
  • Howard P. C., Casciano D. A., Beland F. A. The binding of N-hydroxy-2-acetylaminofluorene to DNA and repair of the adducts in primary rat hepatocyte cultures. Carcinogenesis 1981; 2: 97–102
  • Beland F. A., Kadlubar F. F. Formation and persistence of arylamine DNA adducts in vivo. Environ. Perspect. 1985; 62: 19–30
  • Delclos K. B., Miller D. W., Lay J. O., Casciano D. A., Walker R. P., Fu P. P., Kadlubar F. F. Identification of N2- and C8-modified deoxyguanosine and C8-modified deoxyadenosine as major products of the in vitro reaction of N-hydroxy-6-aminochrysene with DNA and the formation of these adducts in rat hepatocytes treated with 6-nitrochrysene and 6-aminochrysene. Carcinogenesis 1987; 8: 1703–1709
  • Delclos K. B., El-Bayoumy K., Casciano D. A., Walker R. P., Kadlubar F. F., Hecht S. S., Shivapurkar N., Stoner G. D. Metabolic activation of 6-nitrochrysene in explants of human bronchus and in isolated rat hepatocytes. Cancer Res. 1989; 49: 2909–2913
  • Kaderlik K. R., Mulder G. R., Shaddock J. G., Casciano D. A., Teitel C. H., Kadlubar F. F. Effect of glutathione depletion and inhibition of glucuronidation and sulfation on PhIP metabolism, PhIP-DNA adduct formation, and unscheduled DNA synthesis in primary rat hepatocytes. Carcinogenesis 1994; 15: 1711–1716
  • Harris A. J., Shaddock J. G., Manjanatha M. G., Lisenbey J. A., Casciano D. A. Induction of differentially expressed genes in aflatoxin B1-treated Fischer 344 rats and cultured primary rat hepatocytes. Carcinogenesis 1998; 19: 1451–1458
  • McMillan J. M., Shaddock J. G., Casciano D. A., Arlotto M. P., Leakey J. E. A. Effects of dexamethasone on drug metabolizing enzyme activities in primary rat hepatocyte cultures. Mutat. Res. 1991; 249: 81–92
  • Kelly D. W., Holder C. L., Korfmacher W. A., Getek T., Lay J. O., Casciano D. A., Shaddock J. G., Duhart H. M., Slikker W. Metabolism of methapyrilene by Fischer 344 rat and B6C3F1 mouse hepatocytes. Xenobiotica 1992; 22: 1367–1382
  • Feuers R. J., Casciano D. A. Insulin and glucagon's reciprical mediation of a dual regulation mechanism for pyruvate kinase. Biochem. Biophys. Res. Commun. 1984; 124: 651–656
  • Budroe J. D., Shaddock J. G., Casciano D. A. Modulation of ethyl methanesulfonate and 7, 12-dimethylbenz[a]anthracene-induced unscheduled DNA synthesis in the primary rat hepatocyte. Environ. Mutagen. 1987; 10: 129–139
  • Casciano D. A., Schol H. M. Methapyrilene is inactive in the hepatocyte-mediated Chinese hamster ovary/hypoxanthine phosphoribosyl transferase mutational assay. Cancer Lett. 1984; 21: 337–341
  • Pounds J. G., Wright R., Morrison D., Casciano D. A. Effect of lead on cellular calcium homeostasis in the isolated rat hepatocyte. Toxicol. Appl. Pharmacol. 1982; 63: 389–401
  • Pounds J. G., Morrison D., Wright R., Casciano D. A. The effect of lead on calcium-mediated function in the isolated rat hepatocyte. Toxicol. Appl. Pharmacol. 1982; 63: 402–410
  • Shaddock J. G., Chou M. W., Casciano D. A. Effects of age and caloric restriction on cell proliferation in hepatocyte cultures from control and hepatectomized Fischer 344 rats. Mutagenesis 1996; 11: 281–284
  • Shaddock J. G., Casciano D. A. Cryopreservation: A reliable method for long term storage and culture of isolated non-proliferating rat hepatocytes. J. Tissue Culture Methods 1993; 15: 49–52
  • Shaddock J. G., Snawder J. E., Casciano D. A. Cryopreservation and long-term storage of primary rat hepatocytes: effects on substrate-specific cytochrome P450-dependent activities and unscheduled DNA synthesis. Cell Biol. Toxicol. 1993; 9: 345–357
  • Guillouzo A. Liver cell models in in vitro toxicology. Environ. Health Perspect. 1998; 106: 511–532
  • This paper was refereed by J. A. Hinson, University of Arkansas for Medical Sciences, Little Rock, AR 72205.

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