Rough Lipopolysaccharide of Brucella abortus RB51 as a Common Antigen for Serological Detection of B. ovis, B. canis, and B. abortus RB51 Exposure Using Indirect Enzyme Immunoassay and Fluorescence Polarization Assay

References

• 2000 . “ Bovine brucellosis ” . In OIE Manual of Standards for Diagnostic Tests and Vaccines 467 – 474 . Paris : OIE .
   Google Scholar
• Searson , J. E. 1982 . Sensitivity and specificity of two microtitre complement fixation tests for the diagnosis of Brucella ovis infection in rams . Aust. Vet. J. , 58 : 5 – 7 . [PUBMED] [INFOTRIEVE]
   PubMed Web of Science ®Google Scholar
• Marin , C. M. , Jimenez de Bagues , M. P. , Blasco , J. M. , Gamazo , C. , Moriyon , I. and Diaz , R. 1989 . Comparison of serological tests for Brucella ovis infection in rams using different antigenic extracts . Vet. Rec. , 125 : 504 – 508 . [PUBMED] [INFOTRIEVE]
   PubMed Web of Science ®Google Scholar
• Robles , C. A. 1998 . Evaluacion de una tecnica de doble difusion en gel de agar el diagnostico de la infeccion per Brucella ovis en carneros . Vet. Argent. , 15 : 119 – 124 .
   Google Scholar
• Cerri , D. , Ebani , V. V. , Pedrini , A. , Bassi , S. , Bey , R. F. , Andreani , E. and Farina , R. 2000 . Evaluation of tests employed in the serological diagnosis of brucellosis caused by Brucella ovis . New Microbiol. , 23 : 281 – 288 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMedGoogle Scholar
• Riis , D. R. , Hamel , K. L. and Long , D. L. 1984 . Comparison of and enzyme‐linked immunospecific assay (ELISA) with the cold complement fixation test for the serodiagnosis of Brucella ovis infection . NZ Vet. J. , 32 : 18 – 20 . [CSA]
   PubMed Web of Science ®Google Scholar
• Spencer , T. L. and Burgess , G. W. 1984 . Enzyme linked immunosorbent assay for Brucella ovis specific antibody in ram sera . Res. Vet. Sci. , 36 : 194 – 198 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Worthington , R. W. , Weddell , W. and Penrose , M. E. 1984 . A comparison of three serological tests for the diagnosis of Brucella ovis infection in rams . NZ Vet. J. , 36 : 8 – 60 . [CSA]
   Google Scholar
• Worthington , R. W. , Stevenson , B. J. and de Lisle , G. W. 1985 . Serology and semen culture for the diagnosis of Brucella ovis infection in chronically infected rams . NZ Vet. J. , 33 : 84 – 86 . [CSA]
   PubMed Web of Science ®Google Scholar
• Ficapal , A. , Alonso‐Urmeneta , B. , Velasco , J. , Moriyon , I. and Blasco , J. M. 1995 . Diagnosis of Brucella ovis infection of rams with an ELISA using protein G as conjugate . Vet. Rec. , 137 : 145 – 147 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Vigliocco , A. M. , Silva Paulo , P. S. , Mestre , J. , Briones , G. C. , Draghi , G. , Tossi , M. and Nielsen , K. 1997 . Development and validation of an indirect enzyme immunoassay for detection of ovine antibody to Brucella ovis . Vet. Microbiol. , 54 : 357 – 368 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Santos , J. M. , Verstreate , D. R. , Perera , V. Y. and Winter , A. J. 1984 . Outer membrane proteins for rough strains of four Brucella species . Infect. Immunity , 46 : 88 – 194 .
   Google Scholar
• Chin , J. C. , Pang , B. and Carrigan , M. 1991 . Comparison of seroreactivity of rams with brucellosis in a complement fixation test, whole cell ELISA and by immunoblotting . Vet. Microbiol. , 26 : 291 – 299 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Goldbaum , F. A. , Leoni , J. , Wallach , J. C. and Fosatti , C. A. 1993 . Characterization of an 18‐kiloDalton Brucella cytoplasmic protein which appears to be a serological marker for active infection of both human and bovine brucellosis . J. Clin. Microbiol. , 31 : 2141 – 2145 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Estein , S. M. , Baldi , P. C. and Bowden , R. A. 2002 . Comparison of serological tests based on outer membrane or internal antigens for detecting antibodies to Brucella ovis in infected flocks . J. Vet. Diagn. Invest. , 14 : 407 – 411 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Kittelberger , R. , Hilbink , F. , Hansen , M. F. , Ross , G. P. , de Lisle , G. W. , Cloeckaert , A. and de Bruyn , J. 1995 . Identification and characterization of immunodominant antigens during the course of infection with Brucella ovis . J. Vet. Diagn. Invest. , 7 : 210 – 218 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Kittelberger , R. , Hansen , M. , Ross , G. P. and Hilbink , F. 1994 . A sensitive immunoblotting technique for the serodiagnosis of Brucella ovis infections . J. Vet. Diagn. Invest. , 6 : 188 – 194 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Ebani , V. V. , Cerri , D. , Bey , R. F. , Andreani , E. and Farina , R. 2000 . An immunoblotting technique for the serodiagnosis of brucellosis by Brucella ovis . New Microbiol. , 23 : 55 – 62 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMedGoogle Scholar
• Bowden , R. A. , Verger , J. M. , Crayon , M. and Cloeckaert , A. 1997 . Rapid identification of rough Brucella isolates by a latex coagglutination assay with the 25‐kilodalton outer membrane protein and rough lipopolysaccharide specific monoclonal antibodies . Clin. Diagn. Lab. Immunol. , 4 : 611 – 614 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMedGoogle Scholar
• Galanos , C. , Luderitz , O. and Westphal , O. 1969 . A new method for extraction of R lipopolysaccharide . Eur. J. Biochem. , 9 : 245 – 249 . [PUBMED] [INFOTRIEVE]
   PubMedGoogle Scholar
• Schoonjans , F. , Zalata , A. , Depuydt , C. and Comhaire , F. 1995 . MedCalc: a new computer program for medical statistics . Comp. Meth. Progr. Biomed. , 48 : 257 – 267 . [CSA]
   PubMed Web of Science ®Google Scholar
• Carmichael , L. E. and Joubert , J. C. 1987 . A rapid slide agglutination test for the serodiagnosis of Brucella canis infection that employs a variant (M‐) organism as antigen . Cornell Vet. , 77 : 3 – 12 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMedGoogle Scholar
• Myers , D. M. , Jones , L. M. and Varela‐Diaz , V. 1972 . Studies of antigens for complement fixation and gel diffusion tests in the diagnosis of infections caused by Brucella ovis and other Brucella . Appl. Microbiol. , 23 : 894 – 902 . [PUBMED] [INFOTRIEVE]
   PubMedGoogle Scholar
• Chin , J. C. , Plant , J. W. and Claxton , P. D. 1983 . Evaluation of surface components of Brucella ovis as antigens for the detection of precipitin antibody in serums from artificially exposed rams . Aust. Vet. J. , 60 : 264 – 267 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Muncz , F. , Kormendy , B. and Mocsy , M. 1979 . Comparison of agar gel precipitation and complement fixation test for the detection of Brucella ovis infection . Acta Microbiol. Acad. Sci. Hung. , 26 : 11 – 14 . [PUBMED] [INFOTRIEVE]
   PubMedGoogle Scholar
• Webb , R. F. , Quinn , C. A. , Cockram , F. A. and Husband , A. J. 1980 . Evaluation of procedures for the serodiagnosis of Brucella ovis infection in rams . Aust. Vet. J. , 56 : 172 – 175 . [PUBMED] [INFOTRIEVE]
   PubMed Web of Science ®Google Scholar
• Burgess , G. W. and Norris , M. J. 1982 . Evaluation of the cold complement fixation test for diagnosis of ovine brucellosis . Aust. Vet. J. , 59 : 23 – 25 . [PUBMED] [INFOTRIEVE]
   PubMed Web of Science ®Google Scholar
• Myers , D. M. and Varela‐Diaz , V. 1979 . Serodiagnosis of ram epididymitis by counterimmunoelectrophoresis, using Brucella ovis surface R antigen . J. Clin. Microbiol. , 10 : 451 – 453 . [PUBMED] [INFOTRIEVE]
   PubMed Web of Science ®Google Scholar
• Suarez , C. E. , Pancheco , G. A. and Vigliocco , A. M. 1991 . Immunochemical studies on a Brucella ovis specific protein antigen . Vet. Microbiol. , 27 : 125 – 131 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Ajai , C. O. , Cook , J. E. and Dennis , S. M. 1980 . Diagnosing ovine epididymitis by immunofluorescence . Vet. Rec. , 107 : 421 – 424 . [PUBMED] [INFOTRIEVE]
   PubMed Web of Science ®Google Scholar
• Adone , R. and Ciuchini , F. 2001 . Brucella abortus RB51 and hot saline extract from Brucella ovis as antigens in a complement fixation test used to detect sheep vaccinated with Brucella abortus RB51 . Clin. Diagn. Lab. Immunol. , 8 : 119 – 122 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMedGoogle Scholar
• Adone , R. , Ciuchini , F. , Pistoia , C. and Pasquali , P. 2002 . Combined S99/RB51 antigen for complement fixation test for serological diagnosis of brucellosis in cattle and sheep . J. Appl. Microbiol. , 93 : 872 – 876 . [PUBMED] [INFOTRIEVE]
   PubMed Web of Science ®Google Scholar
• Chin , J. C. 1983 . Comparison of different antigenic preparations for the detection of ovine serum antibodies against Brucella ovis by ELISA . Aust. Vet. J. , 60 : 261 – 264 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Rahaley , R. S. , Dennis , S. M. and Smeltzer , M. S. 1983 . Comparison of the enzyme‐linked immunosorbent assay and complement fixation test for detecting Brucella ovis antibodies in sheep . Vet. Rec. , 113 : 467 – 470 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Afzal , M. , Tengerdy , R. P. , Squire , P. G. and Ellis , R. P. 1984 . Characterization of Brucella ovis lipopolysaccharide and its use for diagnosis of ram epididymitis by enzyme‐linked immunosorbent assay . J. Clin. Microbiol. , 20 : 1159 – 1164 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Lee , K. , Cargill , C. and Atkinson , H. 1985 . Evaluation of an enzyme‐linked immunosorbent assay for the diagnosis of Brucella ovis infection in rams . Aust. Vet. J. , 62 : 91 – 93 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Walker , R. L. , LeaMaster , B. R. , Stellflug , J. N. and Biberstein , E. L. 1985 . Use of enzyme‐linked immunosorbent assay for detection of antibodies to Brucella ovis in sheep: field trial . Am. J. Vet. Res. , 46 : 1642 – 1646 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Cardenas , A. L. and Maki , L. R. 1986 . Detection of antibody in rams with contagious epididymitis, using the enzyme‐linked immunosorbent assay . Am. J. Vet. Res. , 47 : 738 – 739 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Riezu‐Boj , J. I. , Moriyon , I. , Blasco , J. M. , Marin , C. M. and Diaz , R. 1986 . Comparison of lipopolysaccharide and outer membrane protein‐lipopolysaccharide extracts in an enzyme‐linked immunosorbent assay for the diagnosis of Brucella ovis infection . J. Clin. Microbiol. , 23 : 938 – 942 . [CSA]
   PubMed Web of Science ®Google Scholar
• Chin , J. C. and Scully , C. 1986 . Identification of immunoreactive membrane antigens of Brucella ovis by ELISA profiling . Res. Vet. Sci. , 41 : 1 – 6 . [PUBMED] [INFOTRIEVE]
   PubMed Web of Science ®Google Scholar
• Cho , H. J. and Niilo , L. 1987 . Diagnostic sensitivity and specificity of an enzyme‐linked immunosorbent assay for the diagnosis of Brucella ovis infection in rams . Can. J. Vet. Res. , 51 : 99 – 103 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Chin , J. C. and Planr , J. W. 1989 . Temporal ELISA response of rams to Brucella ovis following experimental infection or vaccination . Res. Vet. Sci. , 46 : 73 – 78 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Plackett , P. , Corner , L. A. , Fifis , T. , Radford , A. J. , Ripper , J. L. , Chen , N. C. and Liu , Y. M. 1989 . Discrimination between sheep antibodies to Brucella melitensis and to Brucella ovis . Vet. Microbiol. , 20 : 339 – 348 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Nunez‐Torres , E. D. , Diaz‐Aparicio , E. , Hernandez‐Andrade , L. , Trigo‐Tavera , F. J. and Suarez‐Guemes , F. 1997 . Sensitivity and specificity of an ELISA as a screening test for the diagnosis of Brucella ovis in sheep . Rev. Latinoam. Microbiol. , 39 : 123 – 128 .
   PubMedGoogle Scholar
• Kittelberger , R. , Diack , D. S. , Vizcaino , N. , Zygmunt , M. S. and Cloeckaert , A. 1998 . Characterization of an immuno‐dominant antigen in Brucella ovis and evaluation of its use in an enzyme‐linked immunosorbent assay . Vet. Microbiol. , 59 : 213 – 227 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Marin , C. M. , Alonso‐Urmeneta , B. , Moriyon , I. , Perez‐Gomez , S. and Blasco , J. M. 1998 . Comparison of polyclonal, monoclonal and protein G peroxidase conjugates in an enzyme‐linked immunosorbent assay for the diagnosis of Brucella ovis in sheep . Vet. Rec. , 134 : 390 – 394 .
   Google Scholar
• Gall , D. , Nielsen , K. , Vigliocco , A. M. , Smith , P. , Perez , B. , Rojas , X. and Robles , C. 2003 . Evaluation of indirect enzyme immunoassay for presumptive serodiagnosis of Brucella ovis in sheep . Small Rum Res. , 48 : 173 – 179 .
   Web of Science ®Google Scholar
• Velasco , J. , Diaz , R. , Grillo , M. J. , Barberan , M. , Marin , C. , Blasco , J. M. and Moriyon , I. 1997 . Antibody and delayed type hypersensitivity responses to Ochrobactrum anthropi cytosolic and outer membrane antigens in infections by smooth and rough Brucella spp . Clin. Diagn. Lab. Immunol. , 4 : 279 – 284 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMedGoogle Scholar
• Suarez , C. E. , Pacheco , G. A. and Vigliocco , A. M. 1988 . Characterization of Brucella ovis surface antigens . Vet. Microbiol. , 18 : 349 – 356 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Suarez , C. E. , Pacheco , G. A. and Vigliocco , A. M. 1990 . Immunochemical studies of oligosaccharides obtained from the lipopolysaccharide of Brucella ovis . Vet. Microbiol. , 22 : 329 – 334 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Lucero , N. E. , Escobar , G. I. , Ayala , S. , Silva Paulo , P. and Nielsen , K. 2003 . Fluorescence polarization assay for diagnosis of human brucellosis . J. Med. Microbiol. , 52 : 883 – 887 . [PUBMED] [INFOTRIEVE] [CSA]
   PubMed Web of Science ®Google Scholar
• Conde , S. , Nielsen , K. , Capellino , F. , Sierra , V. and Samartino , L. 2002 . 18th Congreso Panamericano de Ciencias Veterinarias . November 2002 , Havana , Cuba. Diagnosis of SRB51 Vaccinated Cattle by Fluorescence Polarization Assay in Serum , pp. 120 (Abstract)
   Google Scholar