41
Views
3
CrossRef citations to date
0
Altmetric
Original Articles

Purification of Glucosyltransferase from Streptococcus sobrinus Cell Culture Medium by Combined Use of Batch Extraction and Countercurrent Chromatography with a Polymer Phase System

, , , &
Pages 2217-2234 | Received 14 Mar 2004, Accepted 31 Mar 2004, Published online: 06 Feb 2007

References

  • Hamada , S. and Slade , H. D. 1980 . Biology, immunology, and cariogenicity of Streptococcus mutans . Microbiol. Rev. , 44 ( 2 ) : 331 – 384 .
  • Loesche , W. L. 1986 . Role of Streptococcus mutans in human dental decay . Microbiol. Rev. , 50 ( 4 ) : 353 – 380 .
  • Koga , T. , Akasaka , H. , Okahashi , N. and Hamada , S. 1986 . Sucrose‐dependent cell adherence and cariogenicity of serotype c Streptococcus mutans . J. Gen. Microbiol. , 132 : 2873 – 2883 .
  • Fukushima , K. , Ikeda , T. and Kuramitsu , H. K. 1992 . Expression of Streptococcus mutans gtf genes in Streptococcus milleri . Infect. Immun. , 60 ( 7 ) : 2815 – 2822 .
  • Shimamura , A. , Tumori , H. and Musaka , H. 1982 . Purification and properties of Streptococcus mutans extracellular glucosyltransferase . Biochim. Biophys. Acta , 702 ( 1 ) : 72 – 80 .
  • Kaseda , K. , Komada , T. , Fukui , K. and Hirose , K. 2001 . A novel approach for purification of recombinant proteins using the dextran‐binding domain . FEBS Lett. , 500 : 141 – 144 .
  • Albertsson , P. A. 1986 . Partition of Cell Particles and Macromolecules New York : Wiley Interscience .
  • Albertsson , P. A. 1958 . Partition of proteins in liquid polymer–polymer two‐phase systems . Nature , 182 : 709 – 711 .
  • Albertsson , P. A. and Nyns , E. J. 1959 . Counter‐current distribution of proteins in aqueous polymer phase systems . Nature (London) , 184 : 1466 – 1468 .
  • Albertsson , P. A. , Sasakawa , H. and Walter , H. 1970 . Cross partition and isoelectric points of proteins . Nature , 228 : 1329 – 1330 .
  • Albertsson , P. A. 1970 . Partition of cell particles and macromolecules in polymer two‐phase systems . Adv. Protein Chem. , 24 : 309 – 341 .
  • Ito , Y. 1988 . Countercurrent Chromatography: Theory and Practice New York : Marcel Dekker .
  • Kendall , D. , Booth , A. J. , Levy , M. S. and Lye , G. J. 2001 . Separation of supercoiled and open‐circular plasmid DNA by liquid‐liquid counter‐current chromatograhy . Biotechnol. Lett. , 23 ( 8 ) : 613 – 619 .
  • Ito , Y. , Yang , F. , Fitze , P. E. and Sullivan , J. V. 2003 . Spiral disk assembly for HSCCC: column design and basic studies on chromatographic resolution and stationary phase retention . J. Liq. Chromatogr. Rel. Technol. , 26 ( 9&19 ) : 1355 – 1372 .
  • Ito , Y. 1987 . Cross‐axis synchronous flow‐through coil planet centrifuge free of rotary seals for preparative countercurrent chromatography. Part I. Apparatus and analysis of acceleration . Sep. Sci. Technol. , 22 ( 8&9 ) : 1971 – 1987 .
  • Ito , Y. 1987 . Cross‐axis synchronous flow‐through coil planet centrifuge free of rotary seals for preparative countercurrent chromatography. Part II. Studies on phase distribution and partition efficiency in coaxial coils . Sep. Sci. Technol. , 22 ( 8&9 ) : 1989 – 2009 .
  • Ito , Y. , Oka , H. and Slemp , J. L. 1989 . Improved cross‐axis synchronous flow‐through coil planet centrifuge for performing counter‐current chromatography. I. Design of the apparatus and analysis of acceleration . J. Chromatogr. , 463 ( 2 ) : 305 – 316 .
  • Shinomiya , K. , Menet , J.‐M. , Fales , H. M. and Ito , Y. 1993 . Studies on a new cross‐axis coil planet centrifuge for performing counter‐current chromatography. I. Design of the apparatus, retention of the stationary phase, and efficiency in the separation of proteins with polymer phase systems . J. Chromatogr. , 644 ( 2 ) : 215 – 229 .
  • Shibusawa , Y. 1996 . “ Separation of proteins by high‐speed countercurrent chromatography ” . In High‐Speed Countercurrent Chromatography Edited by: Ito , Y. and Conway , W. D. Vol. 132 , 385 – 414 . New York : Wiley‐Interscience .
  • Shinomiya , K. , Muto , M. , Kabasawa , Y. , Fales , H. M. and Ito , Y. 1996 . Protein separation by improved cross‐axis coil planet centrifuge with eccentric coil assemblies . J. Liq. Chromatogr. , 19 ( 3 ) : 415 – 425 .
  • Shibusawa , Y. and Ito , Y. 1992 . Countercurrent chromatography of proteins with polyethylene glycol–dextran polymer phase systems using type‐XLLL cross‐axis coil planet centrifuge . J. Liq. Chromatogr. , 15 ( 15–16 ) : 2782 – 2800 .
  • Lee , Y. W. , Shibusawa , Y. , Chen , F. T. , Myers , J. , Schooler , J. M. and Ito , Y. 1992 . Purification of uridine phosphorylase from crude extracts of Escherichia coli employing high‐speed countercurrent chromatography with an aqueous two‐phase solvent system . J. Chromatogr. , 15 ( 15&16 ) : 2831 – 2841 .
  • Shibusawa , Y. 1997 . Lipoproteins: comparison of different separation strategies . J. Chromatogr. B , 699 ( 1&2 ) : 419 – 437 .
  • Shibusawa , Y. , Misu , N. , Shindo , H. and Ito , Y. 2001 . Purification of lactic acid dehydrogenase from crude bovine heart extract by pH‐peak focusing counter‐current chromatography . J. Chromatogr. B , 776 ( 2 ) : 183 – 189 .
  • Shibusawa , Y. , Kihira , S. and Ito , Y. 1998 . One‐step purification of proteins from chicken egg white using counter‐current chromatography . J. Chromatogr. B , 709 ( 2 ) : 301 – 305 .
  • Shibusawa , Y. , Hosojima , T. , Nakata , M. , Shindo , H. and Ito , Y. 2001 . One‐step purification of cholinesterase from human serum by CCC . J. Liq. Chromatogr. Rel. Technol. , 24 ( 11&12 ) : 1733 – 1744 .
  • Shibusawa , Y. , Ino , Y. , Kinebuchi , T. , Shimizu , M. , Shindo , H. and Ito , Y. 2003 . Purification of single‐strand DNA binding protein from an Escherichia coli lysate using counter‐current chromatography, partition and precipitation . J. Chromatogr. , 793 ( 2 ) : 275 – 279 .
  • Yanagida , A. , Isozaki , M. , Shibusawa , Y. , Shindo , H. and Ito , Y. 2004 . Purification of glucosyltransferase from cell‐lysate of Streptococcus mutans by counter‐current chromatography using aqueous polymer two‐phase system . J. Chromatogr. B , 805 ( 2 ) : 155 – 160 .
  • Bhatnagar , M. , Oka , H. and Ito , Y. 1989 . Improved cross‐axis synchronous flow‐through coil planet centrifuge for performing counter‐current chromatography. II. Studies on retention of stationary phase in short coils and preparative separations in multilayer coils . J. Chromatogr. , 463 ( 2 ) : 317 – 328 .
  • Hamada , S. and Torii , M. 1978 . Effect of sucrose in culture media on the location of glucosyltransferase of Streptococcus mutans and cell adherence to glass surface . Infect. Immun. , 20 ( 3 ) : 592 – 599 .
  • Yanagida , A. , Kanda , T. , Tanabe , M. , Matsudaira , F. and Cordeiro , J. G.O. 2000 . Inhibitory effects of apple polyphenols and related compounds on cariogenic factor of mutans streptococci . J. Agric. Food Chem. , 48 : 5666 – 5671 .
  • Tagashira , M. , Uchiyama , K. , Yoshimura , M. , Shirota , M. and Uemitsu , N. 1997 . Inhibition by hop bract polyphenols of cellular adherence and water‐insoluble glucan synthesis of mutans streptococci . Biosci. Biotechnol. Biochem. , 61 ( 2 ) : 332 – 335 .
  • Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature , 227 ( 259 ) : 680 – 685 .
  • Bradford , M. M. 1976 . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein‐dye binding . Anal. Biochem. , 72 : 248 – 254 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.